Rhododendron calophytum belonged to Ericaceae family, Rhododendron genus. It is an endemic evergreen plant with beautiful flowers, and has high ornamental value and breeding value, which is also beneficial to maintain the stability of the ecosystem in Qinling Mountains. Recently, R. calophytum germplasm was endangered due to human being's excessive excavation activities. The genetic diversity of five natural populations of R. calophytum in Qinling Mountain was assessed using inter-simple sequence repeat( ISSR )markers. And 78 bands were amplified by 8 informative and reliable primers, of which 65 were polymorphic loci, the percentage of polymorphic bands was 83%. As analyzed by the software POPGENE, the genetic diversity difference among populations was high with PPL of 82.71%-90.25%, Nei's gene diversity 0.3044-0.4122 and Shannon's Information Index 0.4161-0.5867. Analysis of molecular variance( AMOVA )revealed that among and within population genetic variations accounted for 8.78% and 91.22% of the total genetic variation,respectively. UPGMA cluster analysis based on Nei and Li genetic similarity did not form clades corresponding to geographic distance. Therefore, it is suggested that all the original habitat conditions of R. calophytum populations should be protected as much as possible in order to protect its genetic variation. In addition, we should take some measures of in-situ and ex-situ conservation to protect R. calophytum populations in Zhen'an and Zhashui because these two populations have much higher genetic diversity.]]> 2015/12/14 13:25:17 *, ZHENG Xi-Zi, LI Hou-Hua]]> ZHAO Bing^*, ZHENG Xi-Zi, LI Hou-Hua 9true 2015/12/14 13:25:18 1, GE Ya-Ying², DIAO Huai-Qing³, CAO Li-Ping⁴, YANG Jin-Feng⁴, XIE Yuan-Yuan¹,YANG Jin-Yulu¹, LAI Qi-Xian^4*]]> WU Ling¹, GE Ya-Ying², DIAO Huai-Qing³, CAO Li-Ping⁴, YANG Jin-Feng⁴, XIE Yuan-Yuan¹,YANG Jin-Yulu¹, LAI Qi-Xian^4* 8true Dicranopteris linearis, Woodwardia japonica, Athyrium yokoscense, Stenoloma chusanum, Lygodium japonicum, Asplenium sarelii and Pteris multifida. And we analyzed the diversity of endophytic fungi, using the method of tissue isolation, morphological identification. The results showed that 377 strains of endophytic fungi were isolated from 7 species of plants, and the separation rate ranged from 2.50% to 4.52%. All of 377 strains belong to 25 genera, and among them, Alternaria, Aspergillus, Cladosporium and Verticillium,which are found in all these 7 fern species, indicated that they were dominant, but there were obvious differences in the distribution in each fern species. The total separation rate and the colonization rate of entophytic fungi from leaves are generally greater than that from the rhizomes, but there are some differences in the total separation rate and the colonization rate from different parts of each fern species. The diversity indexes of endophytic fungi range from 0.502 to 0.867 in the seven species of ferns. It indicated that there was high diversity of endophytic fungi in potassium mines and the low similarity of endophytic fungi in different ferns in the same mine areas. The similarity coefficients of the endophytic fungi in the seven species of ferns were from 0.189 to 0.587. The results of this study would not only enrich germplasm resources of plant endophytic fungi, but also would offer basic data for further research on the mechanism that endophytic fungi could strengthen the adaptation of the host plant on mineral nutrition and stress physiology in potassium mine areas.]]> 2016/4/6 22:30:55 *, FAN You-Fu]]> QIAN Xu, GAN Hui-Yun, DU Yong-Tao, ZHANG Xian, CHEN Ye^*, FAN You-Fu 7true Prunus yunnannensis is a perennial deciduous plant in the family Rosaceae. It is an endemic germplasm resource with high ornamental value in Southwestern, China. The species has been widely implicated as an ornamental plant in city landscaping in Kunming, Yunnan Province. In order to reveal the variation degree and variation patterns of the phenotype in P. yunnannensis during resource application and provide scientific evidence for species' breeding and germplasm utilization, we investigated 16 phenotypic traits, including 12 flower characters and 4 foliage traits, of 120 individuals in 6 cultivated populations of P. yunnannensis from Kunming City. Furthermore, the phenotypic traits variations and relationships were also analyzed. In addition, UPGMA based cluster analysis was performed using the phenotypic data. The broad heritability of these phenotypic traits was also calculated in the present study. The results showed that there were significant differences in the phenotypic variation among and within populations. All phenotypic traits had significant differences among the populations except for the phenotypic index of pistil length. Specifically, the coefficient of variation of these phenotypic traits varied from 7.73% to 94.04%, while it varied from 20.74% to 28.22% within populations. The variation of the phenotypic traits was lower within populations than that among populations. The broad heritability of 16 phenotypic traits ranged between 0.54 and 0.97. The leaf length has the highest value, while the pistil length has the lowest value of broad heritability, with the ordering of LL>LW>PETL>PLEF>PW>FBL>FI>FD>PEL>VN>SN>PN>FN>PL>SL>PIL. The correlation analysis showed that there were significant correlation among most phenotypic traits. UPGMA cluster analysis based on phenotypic traits showed that the 6 cultivated populations could be divided into two groups. The reasons that caused the phenotypic traits variation of P. yunnannensis were also discussed.]]> 2016/4/6 22:30:55 1, ZHANG Xue¹, SHEN Shi-Kang^1*, WU Fu-Qin¹, YANG Guan-Song¹, HE Shu-Zhuang², WANG Yue-Hua¹]]> DONG Wen-Jing¹, ZHANG Xue¹, SHEN Shi-Kang^1*, WU Fu-Qin¹, YANG Guan-Song¹, HE Shu-Zhuang², WANG Yue-Hua¹ 6true Isatis indigotica field of Henan Province, five-point random sampling was used and the species and numbers of arthropods on plants and ground were investigated and recorded by net-trapping and visual methods. The results showed that the arthropod species were abundant. There were 2 613 arthropod individuals collected from the field belonging to two classes, ten orders, 39 families and 61 species. Among them, phytophagous group and predatory group all had 24 species(39.34%), four species(6.56%)were classified as parasitoids and nine species(14.75%)were classified as neutrals. The species number of Araneida species were the largest, accounting for 21.31%, which indicated that Araneida species were the most abundant. The individual of Lepidoptera was much larger than that of other orders, accounting for 43.78% of the total number of individuals. The relative abundances of Pieris rapae and Plutella xylostella were 0.184 8 and 0.162 6, respectively, which showed the two species were dominant pests in Isatis indigotica field of Henan Province. It was found that the evenness index of the predatory group was the highest. The dominance index of the parasitoid group was the highest, in which the number of Cotesia vestalis, main parasitic wasps of Plutella xylostella, was the most. It is first time that the arthropod community in Isatis indigotica field of Henan Province was investigated and reported.]]> 2018/6/26 11:36:16 1, LIANG Haiyan²]]> WANG Guochang¹, LIANG Haiyan² 5true Acer ginnala populations and its relationship with geographical ecological factors. We made a comparative study on twelve phenotypic traits of fruits and seeds in seven natural populations of Acer ginnala from seven provinces. The results showed that there were abundant variations in phenotypic traits of fruits and seeds among and within populations of A. ginnala. And there were eleven phenotypic traits except for seed length/seed width(SLW)occurring extremely significant differences among populations and within population. Coefficient of variance(CV)of the twelve phenotypic traits was 8.14%-32.08%, with an average of 13.90%. And the variance coefficient of seeds among populations(8.17%)was lower than that of wing fruits(15.63%), which indicated that the seeds had higher stability than the fruits. Principal component analysis(PCA)showed that the contribution of morphological characteristics of fruits to the phenotypic traits of A. ginnala populations was higher than that of seeds, which indicated that the main variation source of A. ginnala came from the fruits. Mean coefficient of phenotypic differentiation between populations was 35.47%, which was lower than that within populations(64.53%), indicating that the variance within population was the main source of the phenotypic variation for A. ginnala. Twelve phenotypic of seed and fruit traits of A. ginnala were less affected by the geographical ecological factors, which were mainly affected by their own genetic factors. All seven studied populations could be clustered into two groups by UPGMA, but were not strictly classified according to the geography distance, indicating the discontinuity in the variation of twelve phenotypic traits. A. ginnala possessed a high level of phenotypic diversity, which might result from its biological characteristics and large geographical distribution ranges. The multiple variations among and within populations would provide insights and guidelines for the genetic conservation and utilization of A. ginnala resources. At the same time, it is of great significance for further study of genetic diversity, conservation of germplasm resources, rational development of natural forests and cultivation of plantations.]]> 2018/6/26 11:36:16 *]]> WU Yanhong, FAN Zelu, LI Jia, GUO Jinhong, GUO Yakun, WANG Yiling^* 4true Aquilaria sinensis, famous of its fragrant wood, is listed as national Level Ⅱ protected wild plants. For sound conservation this precious population of A. sinensis in Wuguishan District of Zhongshan City, we conducted genetic diversity surveys on it. Because population history such as shrinking or expansion would definitely leave signatures in population genetic variations, based on the genetic diversity investigated in Wuguishan population of A. sinensis, we also made population history inferences for it. Among genetic diversity studies, microsatellite markers are the most suitable tools due to their high polymorphism. Therefore, using eighteen microsatellites obtained by restriction site-associated DNA sequencing(RAD-seq), we examined the genetic diversities of 83 A. sinensis individuals collected from Wuguishan District, and estimated genetic diversity parameters by observed-heterozygosity, expected-heterozygosity and inbreeding index. Because the genetic relationship between individuals could reflect whether Wuguishan population of A. sinensis was derived from a few ancestors, pairwise genetic relatedness was calculated and four types of relationship(parent-offspring, full-siblings, half-siblings and unrelated)between individuals were determined. The population history was inferred for population shrinking(bottleneck)or expansion. Overall, compared to the genetic variation of the species in the same genus and the species with similar life history traits, the genetic diversity in Wuguishan population was slightly low with the observed-heterozygosity and expected-heterozygosity being 0.523 and 0.522, respectively. The value of -0.002 for inbreeding index, which was close to zero, indicated that the whole population was under random mating. Bottleneck analysis indicated bottleneck effects did not happened recently in Wuguishan population, which meant no population shrinking. Pairwise relationships between individuals confirmed this by showing most of individual pairs(>81.810%)were unrelated. Finally, we detected the evidence of population expansion on the basis of allele size distributions. Accordingly, these results indicated that Wuguishan population of A. sinensis is generally genetically healthy. Such information provides the reference for the conservation and the reasonable uses of A. sinensis in the future.]]> 2018/6/26 11:36:16 1, TAN Zongjian¹, LIAO Haobin¹, LIU Panpan¹, SUN Hongmei¹, WANG Ruijiang², CAO Honglin², HUANG Xiangxu², Lai Siru², Liang Dan², WANG Zhengfeng^2*]]> JIANG Qiancai¹, TAN Zongjian¹, LIAO Haobin¹, LIU Panpan¹, SUN Hongmei¹, WANG Ruijiang², CAO Honglin², HUANG Xiangxu², Lai Siru², Liang Dan², WANG Zhengfeng^2* 3true Pinus elliottii × P. caribaea var. hondurensis full-sib families using inter-sequence simple repeat(ISSR)markers. A total of 480 individuals were analyzed using 10 ISSR primers. Nei's unbiased gene diversity in the families ranged from 0.015 2 to 0.087 2. Shannon genetic diversity index values ranged from 0.021 6 to 0.129 4. Only a small proportion(25.65%)of genetic variation resided within families, whereas the majority of genetic variation(74.35%)accounted for the inter-family genetic differentiation index of G_st=0.743 5. On the basis of estimated genetic distance and UPGMA clustering analysis, the genetic differentiation among the eight families was indicated to be relatively high with low gene flow(N_m=0.172 5). The low inter-family gene flow may be related to the high genetic heterozygosity of slash pine and Caribbean pine. These findings are expected to provide a foundation for genetic breeding of Pinus elliottii × P. caribaea var. hondurensis hybrids.]]> 2018/6/26 0:00:00 1, 2*, ZHAO Fencheng^{1, 2}, LI Fuming³, ZHONG Suiying³, TAN Zhiqiang³, WU Huishan^{1, 2}, GUO Wenbing^{1, 2}, LIAO Fangyan^{1, 2}]]> LI Yiliang^{1, 2*}, ZHAO Fencheng^{1, 2}, LI Fuming³, ZHONG Suiying³, TAN Zhiqiang³, WU Huishan^{1, 2}, GUO Wenbing^{1, 2}, LIAO Fangyan^{1, 2} 2true Campanula L. were investigated by inter-simple sequence repeat molecular markers(ISSR). The results showed that 28 of 40 primers can be amplified to clear bands. All the 28 ISSR primers amplified 164 bands, and the proportion of polymorphic fragment was 98.8%. The value of genetic similarity(GS)indexes of seven species based on the ISSR data varied from 0.421 to 0.945, and the highest genetic similarity was 0.945 between C. aristata and C. modesta, which indicated that they had closely relative relationship. Cluster analysis showed that all the species could be divided into four groups: C. colorata, C. cana and C. calcicola in the first group, C. calcicola in the second group, C. crenulata in the third group, and C. aristata and C. modesta in the last group. This study provides reference for the molecular marker assisted identification, resource conservation, flower variety development and breeding of Campanula.]]> 2018/6/26 11:36:16 1,3, QU Jipeng², YANG Zaijun^1*, LU Lu⁴, LIAO Mingli¹, ZHOU Mengxia¹, PENG Zhengsong^1,2]]> BAO Zhiyuan^1,3, QU Jipeng², YANG Zaijun^1*, LU Lu⁴, LIAO Mingli¹, ZHOU Mengxia¹, PENG Zhengsong^1,2 1true