2015/12/15 11:54:15 32true 2015/12/15 11:54:15 31true 2015/12/15 11:54:15 30true 2015/12/15 11:54:15 29true 2015/12/15 11:54:15 28true 2015/12/15 11:54:15 27true 2015/12/15 11:54:15 26true Ranunculus cantoniensis polyploid complex and its allied species was complicated,and hybridization and polyploidization occurred simultaneously in this complex. It was necessary to explore the speciation and dispersal processes of high-ploid taxa. First cloned partial sequence of GBBSI gene,then phylogenetic tree and network were constructed using the introns of GBBSI gene,and proved that the introns of GBBSI gene were suited to study phylogenetic relationship of Ranunculus. The results showed that R. repens closely associated with polyploid complex,participating in the origin and evolution of polyploid complex; R. cantoniensis originated from hybridization of R. chinensis and R. silerifolius var. silerfolius,R. sieboldii originated from hybridization of R. chinensis and R. repens,R. repens might originated from hybridization of R. chinensis and R. silerifolius var. dolicathus; R. chinensis was a key species in this complex,which may play an important role as a pivotal genome.]]> 2015/12/15 11:20:20 *]]> WANG Qun, LI Tong-Jian, HAN Xing-Jie, LIAO Liang, XU Ling-Ling^* 25true ex-situ conservation rare and endangered plant of Kolkwitzia amabilis were studied. The main results were as follows:the anthesis of K. amabilis was about 7 days,the population flowering was about 14 d. Pollen viability achieved the maximum at 2～3 h after flowering,about 90%. After 3 days,no pollen viability was detected. Stigma shown acceptance during the entire anthesis and achieved the maximum at the second day after flowering. The pollen ovule rate(P/O)was 398.1±63.7 and the outcrossing index(OCI)was 3,the results indicated that the breeding system was outcrossing with partial self-compatibility and sometimes pollinators were needed. More than 10 species of insects were observed to visit the inflorescences of K. amabilis. The most common floral visitors were bees and halictids of Hymenoptera,also a small amount of hoverflies of Diptera and a few of bumblebees and lepidopteron,among which Apidae insects were the effective pollinators. No seed was detected in K. amabilis in the ex-situ environment,further study of which is still needed.]]> 2015/12/15 11:20:20 *, ZHOU Ya-Fu, LI Si-Feng, ZHANG Ying]]> MAO Shao-Li^*, ZHOU Ya-Fu, LI Si-Feng, ZHANG Ying 24true Berchemia and Berchemiella (Rhamnaceae)were investigated under SEM and LM. The present study indicated that the drupe of Berchemiella is 1-loculed with one seed,and the drupes are 2-loculed,each locule with one seed in the most species of Berchemia. However,the drupe is 2-locular,but one locule big and with single seed,another locule small and without seed in B. polyphylla. This species may be a transitional taxon between Berchemiella and Berchemia. Seed shape of Berchemia and Berchemiella is usually irregularly long-ellipsoidal. Seed coat sculpture can be divided into three major types,i. e. smooth or almost smooth,indistinctly or sparsely striate with low striae,distinctly striate or sulcate. The differences in seed coat sculpture among species are of taxonomic significance for identifying some species in these two genera.]]> 2015/12/15 11:20:20 1,2, ZHANG Zhi-Yun³, CHEN Yi-Lin⁴, Joongku Lee^5*]]> WEI Ruo-Xun^1,2, ZHANG Zhi-Yun³, CHEN Yi-Lin⁴, Joongku Lee^5* 23true Abies fanjingshanensis and A. yuanbaoshanensis are critically endangered plants. We used chloroplast microsatellite marker to compare the genetic diversity of A. yuanbaoshanensis and A. fanjingshanensis with a widely distributed species A. fargesii var. faxoniana. A total of 21 alleles and 35 haplotypes was detected in 249 individuals from 3 pairs of primer(Pt63718,Pt30204 and Pt71936). The number of haplotypes(No),effective number of haplotypes(Ne)of A. fanjingshanensis(No=12,Ne=3.92)and A. yuanbaoshanensis(No=9,Ne=3.28)were lower than A. fargesii var. faxoniana. A. fanjingshanensis and A. yuanbaoshanensis had less rare haplotypes. The haplotype diversity(He)of A. fanjingshanensis(He=0.75)and A. yuanbaoshanensis(He=0.70)were also lower than A. fargesii var. faxoniana(He=0.97). A. fanjingshanensis and A. yuanbaoshanensis had a relatively low level of genetic diversity at chloroplast microsatellite.]]> 2015/12/15 11:20:20 1,2, ZHANG Guang-Rong¹, QIN Yong-Xian¹, LIUFU Yong-Qing¹, TANG Shao-Qing^1*]]> WEI Fan^1,2, ZHANG Guang-Rong¹, QIN Yong-Xian¹, LIUFU Yong-Qing¹, TANG Shao-Qing^1* 22true Grus nigricollis wintering in Caohai had increased from 223 in 1985 to 506 in 2005,and 1 000 in 2011. And the number of families and genera of the aquatic plant had significantly increased. There were 49 species subjected to 37 genera of 25 families in 2005,in which 11 species and 10 genera and 5 families were new record contrast with 1983. The Comm. of Scirpus yagara,S. tabernaemontani,Leersia hexandra,Juncellus serotinus and Juncus effusus became main dominant communities along the lakeside. Aquatic vegetation had been changing in the direction of marsh vegetation. The speed of Comm. of Alternanthera sessilis spread had been faster than anticipated,particularly seriously in the east,southeast and northeast of Caohai wetland. Interference is the main reason for the change of biodiversity in Caohai. Reducing human disturbance,maintaining a stable surrounding environment of Caohai are important measures to protect and increase the diversity of aquatic plants.]]> 2015/12/15 11:20:20 2, LI Wei-Jie², ZHOU Chuan-Yan^1,2*]]> TANG Jin-Gang², LI Wei-Jie², ZHOU Chuan-Yan^1,2* 21true Cycas rumphii,C. changjiangensis and C. hainanensis were cross-species amplified in genomic leaf DNA of C. fairylakea. The positive PCR productions were cloned and sequenced. Seven loci consistently yielded the predicted size ranges. Of these loci,three were polymorphic and confirmed as simple sequence repeat(SSR)loci in C. fairylakea. They were utilized to investigate the genetic diversity among four wild populations of C. fairylakea. The number of alleles per locus ranged from 2 to 5,and the observed and expected heterozygosities varied from 0.000 to 0.667 and from 0.000 to 0.610,respectively. Pairwise F_st varied from 0 to 0.382. In the whole,C. fairylakea has low levels of genetic variation within populations and high variation across populations. The STRUCTURE analysis indicated that the four C. fairylakea populations could be subdivided into three different hypothetical genetic clusters(K). The BOTTLENECK results suggested that no populations had faced recent significant bottleneck.]]> 2015/12/15 11:20:20 WANG Yun-Hua, LI Nan, CHEN Ting, DENG Huan-Xiang 20true 2015/12/15 11:20:20 1,2, LU Yi³, WANG Ji-Hong⁴, CHEN Xun^3*, LUO Shi-Qin², TANG Jin-Gang²]]> ZHOU Chuan-Yan^1,2, LU Yi³, WANG Ji-Hong⁴, CHEN Xun^3*, LUO Shi-Qin², TANG Jin-Gang² 19true Opisthopapus taihangensis were investigated. Ten ISSR primers were used to amplify 122 individuals of 11 natural populations. Using these primers,150 DNA fragments were produced,and 149 bands were polymorphic loci(PPL=99.33%). The result of POPGENE analysis indicated that genetic diversity of O. taihangensis was higher(I=0.2149,H=0.3455). Daxitian possessed the highest level of genetic diversity while Lingchuan population exhibited the lowest level of genetic diversity. A high level of genetic differentiation among 11 populations was detected based on Nei's genetic diversity analysis(Gst=0.2566,Nm=1.4488). The main factors responsible for the high degree of genetic differentiation among populations may result from habitat fragmentation and barriers of gene flow. Based on genetic information available for O. taihangensis,some conservation strategies proposed.]]> 2015/12/15 11:19:56 1*, ZHAO Li-Xin², LIU Ying¹]]> ZHANG An-Shi^1*, ZHAO Li-Xin², LIU Ying¹ 18true Eulophia zollingeri is one typical saprophytic species in Orchidaceae. Pollination biology of saprophytic orchid has been poorly studied. In order to reveal the significant pollination mechanism of E. zollingeri,broaden our knowledge on reproductive biology of saprophytic orchid,the floral biology and visitation of this plant were studied in Yachang Orchid National Nature Reserve,the west of Guangxi Province,China. E. zollingeri flowers were self-compatible but pollinators were required to achieve effective pollination; Nomia viridicinctula Cockerell was the only effective pollinator; Foraging in the flowers mainly occurs in three periods:8.6% in 9:00-11:30 AM,80.2% in 11:30 AM-14:00 PM,11.2% in 14:00-15:30 PM; The flowers volatilize sweet floral fragrance under strong sunshine in the midday. We considered E. zollingeri attracts pollinators into flowers for foraging by the dizzy floral fragrance and bright yellow color in labellum,then the matchable sizes of flowers and the pollinators in functional morphology provides dynamic support for effective pollination. The pollinators received no reward in the whole pollination procedure,it was food-deceptive pollination mechanism.]]> 2015/12/15 11:19:56 1,2,3, YANG Mei ^2,3, ZHAO Xiu-Hai¹, NI Shi-Dong², YANG Fei-Peng², CHEN Qiao-Qiao², HUANG Bo-Gao^2,3*]]> ZHANG Zi-Bin^1,2,3, YANG Mei ^2,3, ZHAO Xiu-Hai¹, NI Shi-Dong², YANG Fei-Peng², CHEN Qiao-Qiao², HUANG Bo-Gao^2,3* 17true 2015/12/15 11:19:56 *, LEI Xin-Nan]]> FU Cui-Na, XIAO Yuan-Hui, GAN Hai-Feng^*, LEI Xin-Nan 16true 2015/12/15 11:19:56 1, YANG Xue-Ying², NI Ping-Ya³, PEI Li^2*, ZHANG Ying², XU Xiao-Yu²]]> SONG Bing-Ke¹, YANG Xue-Ying², NI Ping-Ya³, PEI Li^2*, ZHANG Ying², XU Xiao-Yu² 15true Clematis(Ranunculaceae),C. dongchuanensis,is described as new from northeastern Yunnan. In having simple leaves,erect sepals and puberulous filaments and anthers of stamens this species is related to C. repens Finet & Gagnep.,and from the latter differs in its cordate-ovate and coarsely dentate leaves,dichotomous cymes,and obtuse,not apiculate apexes of anthers.]]> 2015/12/15 10:55:20 WANG Wen-cai 14true 2015/12/15 10:55:20 1,2, HAN Xing-Jie², LI Tong-Jian², XU Ling-Ling², LIAO Liang^2*]]> LIU Yu-Ling^1,2, HAN Xing-Jie², LI Tong-Jian², XU Ling-Ling², LIAO Liang^2* 13true Phormidium strains picked up from Suzhou districts and 12 other strains selected from Genebank as the materials to study the characteristics and the evolutionary relationships among different types of copy. The results showed that the PCR product of ITS of Phormidium had single band(i. e. ITS-IA type or ITS-I type)or two bands(i. e. ITS-IA and ITS-N type),and ITS-IA was the most popular type in the materials used in this study; the phylogeny analyzed results indicated that ITS-IA type and ITS-I type were distributed in different groups respectively,and the ITS-IA group appeared earlier than ITS-I group. Thus we proved that ITS-I type was evolved from a deletion of tRNA^Ala coding region of ITS-IA type,and ITS-IA type should be the basic structure of ITS copy of Phormidium.]]> 2015/12/15 10:55:20 *]]> JIAO Shu-Jing, HUANG Xian-En, GU Qing, SHI Quan-Liang^* 12true Nervilia fordii and its common adulterants,matK genes from N. fordii and its adulterants were amplified and sequenced using a pair of universal primes. The results revealed that matK genes acquired from N. fordii and its adulterants contained 587 base pairs,and average GC content was 32.8%. The intraspecific distances among N. fordii were 0,whereas the interspecific distance between N. fordii and its adulterants ranged from 0.016 to 0.375. The clustering tree according to Neighbor Joining method and Kimura 2-Parameter model discriminated N. fordii and its adulterants obviously. matK gene is suggested to be an effective biomarker to identify N. fordii and its common adulterants.]]> 2015/12/15 10:55:20 *]]> HUANG Qiong-Lin, LIANG Ling-Ling, HE Rui, ZHAN Ruo-Ting, CHEN Wei-Wen^* 11true 2O₂-CH₂COOH maceration. The results were as followed: the length of fiber in the lower side of the upright branches at the bottom was longer than the upper side significantly,the length of fiber in the upper side of the pendulous branches at the bottom was longer than the lower side significantly. There were no significant differences in other positions; the fiber diameter at different positions among four varieties indicated no significant differences separately.The results showed that the diferences of fiber size effects shoot development of Prunus mume.]]> 2015/12/15 10:55:20 *]]> WANG Fu-Ting, YANG Wei-Ru, HAO Rui-Jie, WANG Tao, ZHANG Qi-Xiang^* 10true Liriodendron chinense provenances as materials,the 16 anatomical traits of leaf epidermis,leaf cross section and main vein cross section were observed and variance analyzed. The results showed that there were abundant variances in leaves anatomical traits among/within L. chinense provenances. Highly significant differences existed in all anatomical traits among/within provenances except the lower epidermis density among provenances. The mean phenotypic differentiation coefficient was 27.5%; this indicated the variation of main leaves anatomical traits within provenances(72.5%)was obviously higher than that of traits among provenances. The correlations among the anatomical features,geography and climate were also analyzed. The results indicated that the variations among provenances had the gradient regularity,and there were negative correlations between all epidermis traits and longitude and mean annual temperature. Most of traits of leaves and main vein cross section there were positive correlations with longitude and latitude,however,most of them had negative correlations with mean annual precipitation and mean annual temperature. According to the UPGMA cluster analysis based on the Euclidean distance,the five L. chinense provenances could be divided into three groups.]]> 2015/12/15 10:55:20 *, ZHU Li-Qiong, HUANG Shou-Xian, WEN Xiang-Feng]]> ZHAO Li-Jun^*, ZHU Li-Qiong, HUANG Shou-Xian, WEN Xiang-Feng 9true Paris were evaluated. Applied researches in genetic diversity of plant in Paris were carried out based on SCOT markers. Genomic DNA polymorphic analysis in total forty individuls of nine species in Paris were performed by the approach of start codon targeted polymorphism(SCOT). The genetic diversity of the nine species in Paris was high in Sichuan Province. Clustering analysis could be used to study the classification of Paris,which indicated that forty individuls were clustered 4 classes. P. polyphylla var. stenophylla was clustered alonely. P. fargesii,P. delavayi var. petiolata,P. delavayi var. delavayi,P. polyphylla var. yunnaensis were clustered. P. thibetica and P. polyphylla var. chinesis were clustered. P. axiparis var. axialis and P. mairei were clustered. In conclusion,plant in Paris was identified accuracily in molecular by SCOT markers and molecular evidences were provided on the taxonomic status of species and interspecific. It is helpful to further study on gene mapping of P.]]> 2015/12/15 10:55:20 1, XIN Ben-Hua¹, YANG Hua², LIU Chen³, TIAN Meng-Liang^3*]]> LI Zhuang¹, XIN Ben-Hua¹, YANG Hua², LIU Chen³, TIAN Meng-Liang^3* 8true Pinus crassicorticea is a rare tree species has high economical value in northwestern Guangxi. As the habitat has been seriously damaged,P. crassicorticea is now regarded as an endangered species. To preserve the germplasm of this species,the genetic diversity of P. crassicorticea was quantified based on 7 SSR loci. The interspecies relationship between P. crassicorticea and two sympatric pine species,P. yunnanensis var. tenuifolia and P. massoniana,was further analyzed. The results showed that the mean number of effective alleles(Ne)per locus was 1.653,the average observed heterozygosity(Ho)was 0.577,the expected heterozygosity(He)was 0.374,Shannon's information index(I)was 0.540 and mean Nei's gene diversity(h)was 0.376,indicating that P. crassicorticea maintains high levels of genetic variation. The genetic identity(GD)was 0.0175 between P. crassicorticea and P. massoniana,while 0.0525 between P. crassicorticea and P. yunnanensis var. tenuifolia. The interspecies relationship coefficient was 0.094 within P. crassicorticea and P. massoniana,and 0.066 in P. crassicorticea and P. yunnanensis var. tenuifolia, respectively. Therefore,it was reasonable that P. crassicorticea was genetically closer to P. massoniana than P. yunnanensis var. tenuifoli. The strategy for the genetic resource conservation of P. crassicorticea was also discussed in this paper.]]> 2015/12/15 10:55:20 1,2, WU Dong-Shan³, WANG De-Jun¹, YANG Zhang-Qi², LI Huo-Gen^1*]]> FENG Yuan-Heng^1,2, WU Dong-Shan³, WANG De-Jun¹, YANG Zhang-Qi², LI Huo-Gen^1* 7true Pellionia(Urticaceae),P. mollissima,is described as new from southwestern Guangxi. This species is characterized by its lineolate and tuberculate achenes,and by this unique fruit character it may be distinguished from all the other species of Pellionia.]]> 2015/12/15 10:10:33 WANG Wen-Tsai 6true 2015/12/15 10:10:33 1*, WANG Yan-Ping^1,3, CHEN Qin², ZHOU Zhi-Qin³]]> ZHOU Jun-Hui^1*, WANG Yan-Ping^1,3, CHEN Qin², ZHOU Zhi-Qin³ 5true Pinus yunnanensis var. tenuifolia. The results showed that 13 sites and 37 alleles were detected. For every site,the average value of observation allele number(A)was 2.85,polymorphism rate was 100% and the average values of effective number of alleles(Ne)was 1.45. The average value of effective number of alleles was 1.447 within each population. P. yunnanensis var. tenuifolia had a low genetic diversity level among the three populations, the average observed heterozygosity(Ho)was 0.341 and the average expected gene heterozygosity(He)was 0.281. Nei's gene diversities(h)at population level were 0.256 and 0.297. Shannon's information indexes(I)at population level were 0.448 and 0.484. In addition, the three populations deviated from Hardy-Weinberge quilibrium. The average coefficient gene differentiation(Gst)was 0.089. Most of variation appeared inner population and there was not gene differentiations among populations. The range of gene flow of different gene polymorphism sites was 4.693-122.189,the average number was 11.17,which indicated that the habitat of P. yunnanensis var. tenuifolia was limited and resulted in the limited natural variation,but there were abundant gene exchanges between each population.]]> 2015/12/15 10:10:33 1, FENG Yuan-Heng¹, WU Dong-Shan²]]> YANG Zhang-Qi¹, FENG Yuan-Heng¹, WU Dong-Shan² 4true Glehnia littoralis F. Schmidt ex Miquel in China. Among them,seven populations were wild plants and one was cultivar plant. With eight SRAP primer combinations,168 loci were identified. Among them,118 were polymorphic and accounted for 70.23% of total amplified loci,showing a high polymorphism. The genetic similarity among these eight populations ranged from 0.830 6 to 0.983 6 and the genetic diversity was 0.0165-0.1856. Based on the results of cluster and principal coordinate analysis,the eight populations of G. littoralis were divided into three groups. The population from Dalian of Liaoling Province was confirmed as Group A, while populations from Shandong Province(excluding Rizhao)were clustered into Group B. Group C comprised populations from Rizhao of Shandong Province to Shenzhen,Guangzhou.]]> 2015/12/15 10:10:33 *, ZHOU Yi-Feng, WU Bao-Cheng, DONG Xiao-Yu]]> SONG Chun-Feng, LIU Qi-Xin^*, ZHOU Yi-Feng, WU Bao-Cheng, DONG Xiao-Yu 3true Wedelia trilobata,W. chinensis and their hybrid under shade and full light have been investigated using scanning electron microscope(SEM,JSM-6360LV)and transmission electron microscope(TEM,JEM-1010). In contrast to full light treatment,the primary fully expanded mature leaves of the three species under shade treatment had reduced stomatal density of both upper and lower epidermises,and smaller leaf thicknesses and thicknesses of upper and lower epidermises,palisade tissue and spongy tissue. Moreover,the chloroplast of the primary fully expanded mature leaves,the stacking of grana lamellae increased and the number of the starch grain increased. However,the effects of shade on the microstructure of the senescent leaves were different to that found in primary leaves. For hybrids,the thicknesses of the upper epidermis,the palisade tissue,the spongy tissue and the whole leaves increased. Also,the thickness of the lower epidermis of the invasive species(W. trilobata)and the thickness of the upper epidermis of the native species(W. chinensis)increased. Moreover,the ultrastructure of their chloroplasts had more severe impairment under shade treatment,e.g. the grana lamellae had edge-lysis. Conclusively,the response in leaf microstructure and chloroplast ultrastructure varied among different Wedelia species and different leaf developmental stages within the same species; the hybrid showed intermediate response between its parent species.]]> 2015/12/15 10:10:33 1,2, HU Xiao-Ying¹, WEI Xiao³, YE Wan-Hui¹, SHEN Hao^1*]]> YI Li^1,2, HU Xiao-Ying¹, WEI Xiao³, YE Wan-Hui¹, SHEN Hao^1* 2true Metasequoia glyptostrobides planted in Yaan,Sichuan were evaluated. The results were as follows:the growth ring width first increased from pith to 10-year old,then decreased,and the main wood formation period was 15 a. The tracheid length of late wood was longer than that of early wood,and the tracheid length increased with the growth age,then decreased after 13-year old. The determined values for tracheid width,cell wall thickness,and lumen diameter were 44.6,7.23,37.48 μm,respectively,and significant difference in these index were found between early and late wood. Length to width ration(L/W),cell wall thickness to lumen diameter(2T/D),and lumen diameter to width(L/W)was 95.40,0.27 and 0.82,respectively. The results obtained in this study indicated that M. glyptostrobides wood was available for pulping.]]> 2015/12/15 10:10:33 QI Jin-Qiu, HAO Jian-Feng, XIE Jiu-Long, WU Bing-Ling, LUO Hao 1true