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Elatostema duyunense,a new species of Urticaceae from Guizhou,China

2016/1/19 17:51:31

A new species of Elatostema(Urticaceae)are described from Guizhou, China.

Pollen characteristics of twenty-eight species of Saussurea in alpine meadow of the eastern fringe of the Qinghai-tibetan Plateau

2016/1/19 17:51:31

Pollen morphology of 28 species of Saussurea in alpine meadow of the eastern fringe of the Qinghai-Tibetan Plateau was observed by scanning electron microscope and compared. Results show that the pollen morphology in this genus is highly unanimous. The pollen grains are sub-spherical or prolat with 3 colpora,3 lobate circular in polar view. By the ornamentation,the pollen grains of the genus can be divided into 4 types,fovedate,granules,reticulate-granules and reticulate. Some interspecific differences exist in exine ornamentation,colpus width,spinulate size,distribution density and pollen size. Based on cluster analysis,the pollen morphology of this genus could be divided into five groups belonging to subgen.Eriocoryne,subgen.Amphilaena,subgen.Theodorea and subgen.Saussurea respectively.

Observation on the double fertilization in rice by using confocal microscopy

DAI Xi-Mei, HUANG Qun-Ce, QIN Guang-Yong — 2016/1/19 17:51:31

Nucleus fluorescent staining and whole clearing technique was first used in this study to observe the process of the double fertilization in rice by using Laser Scanning Confocal Microscopy(LSCM). LSCM is a powerful tool in studying the structure of tissues and cells because of the function of“CT Scanning”. When the sample,which was stained with nucleus fluorescent and cleared with whole clearing technique,was excited by 488 nm laser,the nucleus and nucleolus of the cells in it excited bright fluorescent color and the profile of the nucleus was very clear. And the LSCM image showed perfectly the structure and special position of the cells in the embryo sac. Not only the images of small embryo sacs before fertilization, but also the images of big embryo sacs during and after fertilization were perfect. The results suggested that the LSCM was an ideal tool to study the process of double fertilization compared to traditional photics microscope.

Megasporogenesis and microsporogenesis as well as male and female gametophyte development of Pittosporum tobira

2016/1/19 17:51:31

This paper describes megasporogenesis and microsporogenesis as well as male and female gametophyte development of Pittosporum tobira(Thunb.)Ait. by using nomal paraffin method. The results are as follows:(1)cytokinesis in the process of meiotic division of microsporocyte is successive type,the main arrangement of microspores tetrad is tetrahedral, and wall of some microspores are shriveled at the last stage of microspores tetrad;(2)the anther wall is composed of 4 layers,which are epidermis,endothecium,middle layer and tapetum;(3)an ovary locules has many ovules,which are unitegmic,tenuinucellate and anatropous. Four megaspores divided from megasporocyte arrange in line during tetrad period,and some in decussate,the chalazal megaspore is functional;(4)the development of embryo sac was polygonum type which belongs to the bisporic embryo sac.Most mature embryo sac are 5-nucleated embryo sac which the 5 nuclei belong to 4 cells.

Spermodermal micro-morphology of Bauhinia ( Leguminosae: Caesalpinioideae )

2016/1/19 17:51:31

The seeds of 21 species(including infraspecific taxa)in Bauhinia were studied by LM and SEM. The colors,shapes and the ornamentations of these seeds are various. Some association was found in hilum position,aril-lobes,pseudolens and the ornamentation of seed surface,viz.,in the species without aril-lobes,pseudolens is indistinct in the surface and the ornamentation is reticulate,the position of hilum is subapical rather than apical. And the ornamentations of most seeds in subgenus Bauhinia are rugulose while in subgenus Phanera being reticulate.

Study on the vessel elements and tylosis of secondary xylem in Averrhoa carambola

TANG Wei-Ping, CHEN Shu-Si — 2016/1/19 17:51:31

The vessel elements of secondary xylem in Averrhoa carambola are observed by bio-microscope image analysis system and micrography. Three types of vessel elements were found,a tail on each side,a tail on one of the side and no tail. Two types of perforation plates were present as follows: both ends of the vessel element are simple perforation plates; one end simple perforation,the other multiple perforation,in which there are two simple perforations. The 48 percent of vessel elements have vesicular tylosis. The intervascular pitting is alternate pitting.

Mosses flora of Red River in Guizhou Province

2016/1/19 17:51:31

The Red River Region has not been investigated systematically. In 2003 and 2004,the author and companions made three bryophytic surveies in this region,and about 500 specimens of bryophytes were collected there. From all these specimens,36 families,78 generas and 204 species(including 8 subspecies,4 varieties and 1 form)are identified,including 5 newly recorded species in Guizhou. According to“The areal-types of Chinese genera of seed plants”by Wu Zheng-yi,13 type of the bryoflora in this region are recognized. Tropical elements is the most important ones. For considering the relationships between the mossflora of low mountain valley monsoon forest and other mountains, the author selected nine regions. Comparing the similarity coefficient of moss genera and species between this region and other mountains, this region is very close to the Mt.Maolan. While the similarity coefficient between this region and Mt.Changbai is the lowest,so the relation between them is very far.

Study on floristic phytogeography of seed plants in the Pamirs of China

2016/1/19 17:51:31

The flora of Pamirs consist of 963 species of seed plants belonging to 59 families and 303 genera. Based on the analysis of the floristic elements at family,genera and species level,there are some conclusions as follows:The floristic elements of the Pamirs of China are complex. Almost all kinds of distribution types are more or less represented here. Among the genera distribution patterns,the floristic elements with 83.8% temperate genera and 4.3% tropical ones reveal dominant temperate property. And in the species distribution patterns,the flostric element with 75.1% temperate species and 1.8% tropical ones indicate that the flora is temperate in nature flora. From the level of families and genera,predominant families are minor-genera family and one-genera family. There are only three numerous-genera family which is more than 20 genera,but the number of genera and species auounts for 35.9% and 38.6% respectively to the total,so phenomenon of the flora is obviously; single-species or minor-species genera is more,the ratio of genera and species is high,so composition of floristic is complex.

Effects of dwarf bamboo on the regeneration of dominant tree species in gaps of subalpine dark coniferous forest

2016/1/19 17:51:31

The effects of gap size and dwarf bamboo coverage on the regeneration of subalpine dark coniferous forest were examined in Wolong Giant Panda Nature Reserve,Sichuang Province. The gaps(≤50 m²,50-150 m² and>150 m²)were selected, there are 3 dwarf bamboo coverage(20%,20%-50% and>50%)in each gap area type,and three contrasting understory bamboo cover conditions(≤20%,20%-50% and>50%)were contained in the same sized gaps,as well. The results showed that:(1)no matter what gap area,the number of tree seedlings in gaps was more than that in understory,gap regeneration is the primary approach of the community regeneration of Abies faxoniana;(2)the gaps which we selected were all at early phase,so the specific composition and quantity of seedlings among every gap are obviously different. The seedlings and saplings abundance of Betula utilis increased with gap enlarged,and this demonstrated that it needed larger gaps to regenerate than Abies faxoniana;(3)there were significant differences in number of seedlings among the gaps with different bamboo coverage,and dense bamboo evidently impeded the forest regeneration process;(4)with the increasing of gap size,the degree of dispersion of Fargesia nitida decreased,and average height and basal diameter tended to increase.

Species diversity change pattern in gap gradient in karst forest in Maolan Nature Reserve, Guizhou Province

LONG Cui-Ling — 2016/1/19 17:51:31

To research the species diversity change pattern in gap gradients. The Margalef index,Shannon-Wiener index and Pielou evenness index were computed with the data from gaps investigation in karst forest in Maolan Nature Reserve. The results showed that species composition was different greatly in different gap area from gap center to non-gap. Some light-demanding species such as Platycarya longipes was much in gap center area,shade-tolerant species such as Quercus glarca was much in non-gap area and moderate shade-tolerant species such as Lindera communis and Ilex latifolia was much in near gap center and gap border area. Species richness index gradually increased from non-gap to the gap center area,such that:Non-gapMiddle phase gap>Late phase gap and Large size gap>Middle size gap>Small size gap. Early phase gap and large size gap had remarkable edge effect.

Preliminary study on population stucture and its dynamic development of Pentaphylax euryoides in the hilly country of Northeast Guangxi

2016/1/19 17:51:32

Based on the data of representative sampling in four representative sample plots in Pentaphylax euryoides population distribution area,the structure,distribution patterns and dynamic development of P.euryoides population were studied by using mathematical analysis method. The result indicated that the importance value of P.euryoides was far higher than other species in these communities,its dominance degree was outstanding,and its distributing pattern was aggregated distribution. This was mainly decided by effect of environmental factors. The number of young trees was large,and the age structure of P.euryoides was developing.

Liana diversity and abundance of a tropical montane rainforest in Mengsong, Southern Yunnan, China

CHEN Ya-Jun, WEN Bin — 2016/1/19 17:51:32

Species richness, abundance, size-class distribution, climbing mode and the percentage of trees(DBH≥5 cm)infested by lianas were investigated in valley and ridge plots in a tropical montane rain forest in Mengsong,southern Yunnan. A total of 64 liana species representing 30 families were recorded. There were averages of 95.7 and 57 lianas with dbh≥1 cm in the valley and in the ridge per 0.1 hm²,respectively. A greater proportion of lianas was twiners(57% of density)while 3% of all were tendril climbers,reflecting the late successional stage of the forest. Approximately 43.7% and 28.6% of trees(dbh≥5 cm)were liana-infested in the valley and ridge plots. Compared to other tropical Asian tropical forests,the density of lianas in Mengsong are very high though the diversity of species is relatively low. There are also some huge lianas in some plots,which is the most conspicuous characteristics of a mature tropical rain forest.

Community characteristics of Rhododendron simiarum in Shimentai Nature Reserve, Guangdong

2016/1/19 17:51:32

Community characteristics of Rhododendron simiarum in Chuandiding,Shimentai Nature Reserve were studied. The results show that 106 vascular plant species belong to 46 families and 75 genera in a sampling area of 2 500 m². The dominant families of community are Rosaceae,Ericaceae,Fagaceae,Lauraceae,Aquifoliaceae and Theaceae,etc. The dominant species of the community are Rhododendron simiarum,Pinus kwangtungensis,Schima remotiserrata,Ilex lohfauensis,Dendropanax chevalieri,Podocarpus brevifolius,Olea dioica,Tsuga techekiangensis and Pentaphylax euryoides,etc. The species indexes of Simpson and Shannon-Wiener in the community are 0.941 and 4.977,respectively. Most of the floristic elements of family for seed plants are Pan-tropical and North Temperate distributing type,and main genera are Pan-Tropical and tropical-Asia areal-types,which show the characteristic of transition of south subtropical and mid-subtropical elements. Analysis on the age structure indicated that the population of Rhododendron simiaru has an increasing trend,but Pinus kwangtungensis expresses a decreasing stage. Pseudotaxus chienii and Podocarpus brevifolius will develop a big population because of the large amount of seedling in the generation layer.

Study on intraspecific and interspecific competition of Pinus taiwanensis

2016/1/19 17:51:32

Pinus taiwanensis is a common species,and it is one of the dominant species in subtropical area. The intraspecific and interspecific competitions of P.taiwanensis were analyzed quantitatively by using the improved competition index. The results show that with the increase of the size of objective tree and the distance between plants,the intraspecific competition intensity decreases,because of the population density regulation during self-thinning. In the community,there are many other species,but their diameter at breast height is less than that of P.taiwanensis,as a result,the competition intensity of intraspecific was more excessive than that of interspecific. The order of competition intensity is P.taiwanensis—P.taiwanensis>Cunninghamia lanceolata—P.taiwanensis>Quercus glandulifera—P.taiwanensis>other species—P.taiwanensis. The relation between competition intensity and individual growth of objective tree follows closely the following equation CI=AD^-B,and the changing of competition intensity is very small when the diameter of the objective tree grows to 20 cm. The model can simulate and predict the intraspecific and interspecific competition efficiently. The study provides a new approach to measure the competition intensity and to ascertain the neighborhood zone on studying the plant competition.

Dynamic distributes of endophytic fungi from Camellia sinensis

YOU Jian-Ming — 2016/1/19 17:51:32

143 strains of endophytic fungi belonging to 14 genera were isolated and morphologically identified from the roots, stems and leaves of tea. Among which 62 strains of 11 genera were isolated from roots, 55 strains of 9 genera from stems, and 26 strains of 6 genera from leaves. The results showed that the quantity, population, and distribution of the endophytic fungi varied in different part of the plant Camellia sinensis.

Phenophase observation and introduction and cultivation of ornamental ferns

ZENG Han-Yuan, QIU Kun, ZHANG Qing-Zheng, XIAO Hong — 2016/1/19 17:51:32

The introduction and culture of 30 ferns are researched. They are Isoetes sinensis,Angiopteris fokiensis,Osmunda vachellii,Pteris wallichiana,Asplenium ensiforme,Athyrium caudiforme,Matteuccia orientalis,Dryopteris uniformis,Polystichum dielsii,etc. The results showed:(a)the introduced ornamental ferns have very high survival rate;(b)they can be supervise easily;(c)most of them are evergreen and graceful,and have higher ornamental value. They can overwinter safely in Huaihua city so long as the temperature below zero in winter which never last 1 week or more. Hence,these ferns are worth exploiting in subtropical zone;(d)nine ferns(Osmunda vachellii,Alsophila metteniana,Athyrium otophorum,Athyrium iseanum,Athyrium caudiforme,Ctenitopsis sinii,Dryopteris uniformis,Polystichum dielsii,Cyrtomium balansae)yield sporangium as soon as they expand leaf;(e)the sporangium of most of the ferns becomes ripe after 30-40 days'development;(f)the periods of foliage leaf germination and expansion will be postponed half a month also if they are introduced and cultivated after germinating. But the appearance time and ripeness time of sorus remains changeless;(g)the sorusperiods of different species are different. Nevertheless,they are relative stable in the same species and therefore can be used as one of the evidences of differentiation. We propose that the ferns sorus periods should be put in the revisal Flora Reipublicae Popularis Sinicae and local floras.

Effect of STS on biological and physiological changes of the tree peony

2016/1/19 17:51:32

The tree peony(zhu sha lei)in green period was treated with different concentrations of STS(0,0.5,1.0,1.5 mmol/L). The changes of SOD activity,the producing velocity of superoxide anion,soluble protein content,respiratory rate and MDA content were measured,the effects of the florescence with different treatments were observed and recorded. The results indicated that the different concentrations of STS can increase SOD activity and soluble protein content in the petals,reduce the producing velocity of superoxide anion,respiratory rate and MDA content,and the florescence was prolonged obviously with different concentrations of STS. The 1.0 mmol/L is the best.

Study on preservation in vitro of Morinda officinalis

LI Feng, FU Chuan-Ming, HUANG Ning-Zhen, ZHAO Zhi-Guo, TANG Feng-Luan — 2016/1/19 17:51:32

Effects of the mineral nutrition,sucrose and plant regulator(CCC,PP₃₃₃,ABA,MH)in different concentrations on preservation in vitro of Morinda officinalis were examined. The results showed that tube plantlets of Morinda officinalis can be preserved for 360 d on MS,1/2MS and 1/4MS medium,with survival rate of 90%. When the concentration of sucrose in the medium was 20～40 g/L,the plantlets grown well and could still be longly preserved. Their lateral buds were induced while the growth of top buds and leaves were inhibited by these regulators. Medium with PP₃₃₃ was good for producing intact vigorous plantlets. On PP₃₃₃0.5～1.0 mg/L+1/2MS,the tube plantlets could be conserved for 480 d with 100% survival rate.

Growth response and threshold concentration of Agrostis stolonifera to Cu²⁺, Zn²⁺,Cd²⁺or Pb²⁺ stress

2016/1/19 17:51:32

By sand cultural method,the experiment was conducted to investigate growth response and threshold concentration of Agrostis stolonifera to Cu²⁺,Zn²⁺,Cd²⁺ or Pb²⁺ stress. It was found that rate of seed germination decreased with increasing supply of Cu²⁺,Zn²⁺,Cd²⁺ or Pb²⁺. Heavy metal supply at concentration ≤100 mg/L increased plant height of A.stolonifera,whereas Cu²⁺,Zn²⁺,Cd²⁺or Pb²⁺supply higher than 100 mg/L decreased plant height. Cu²⁺ inhibited root growth significantly,and root length decreased with increasing Cu²⁺ supply. Compared with the control,root length of A.stolonifera was lowered by 93.75% at Cu²⁺ concentration of 600 mg/L. Aboveground biomass increased at Cu²⁺,Zn²⁺ or Pb²⁺ concentration ≤200 mg/L,but decreased with increasing concentrations when their concentrations were higher than 200 mg/L. Chlorophyll content increased at Cu²⁺,Zn²⁺ concentration ≤100 mg/L or Cd²⁺,Pb²⁺ concentration ≤200 mg/L,but tended to decrease at Cu²⁺,Zn²⁺ concentration ≥100 mg/L or Cd²⁺,Pb²⁺ concentration ≥200 mg/L. Only Cd²⁺ significantly lowered chlorophyll content of A.stolonifera by 43.55% at concentration of 600 mg/L. Synthetical effect of heavy metal on A.stolonifera growth indicated that A.stolonifera was most sensitive to Cu²⁺ stress with low threshold,but the effect of Zn²⁺ stress on A.stolonifera growth was the least.

Construction of pepper CaNPR1-RNAi expression vector and its transformation to pepper

2016/1/19 17:51:32

Full-length cDNA of CaNPR1,a homologous gene of the positive regulatory gene NPR1 in systemic acquired resistance of Arabidopsis thaliana,was cloned from pepper(Capsicum annuum). CaNPR1 and NPR1 share 62.9% homology at mRNA level and their encoded products have 49.7% identity and 65.7% similarity. In order to identify the function of CaNPR1 in SAR of pepper, an expression vector pCaNPR1-RNAi for silencing CaNPR1 in pepper was constructed. Pepper cultivar Guiyan5 was transformed with pCaNPR1-RNAi by Agrobacterium tumefaciens-mediated transformation. Six kanamycin-resistant transgenic seedlings were obtained. Southern analysis confirmed that all the six seedlings carried the introduced transgene.

Histological study on the somatic embryogenesis and development of Saposhnikovia divaricata

2016/1/19 17:51:32

Paraffin slice and semithin section are utilized to study the characteristics of morphogenesis and anatomical structure during the occurrence and development of somatic embryo in tissue culture of Saposhnikovia divaricata. The results show:(1)there are lots of starch in the embryogenetic cell,and gradually decreased accompanying the cell fission and differentiation;(2)the somatic embryogenesis and development experience all the stages of zygote embryo;(3)the multicellular embryo is developed from periclinal division primarily,and accordantly grows regularly;(4)there is distinct boundary between the different stages of somatic embryo and the cells around;(5)the suspensor isn't obvious;(6)the abnormal joined embryo is caused by the high density of embryonic cells, and the subordinate embryo is caused by the new occurrence center produced by the original embryo.

Study on the pathogenicity of Cryphonectria parasitica by 2-DE

2016/1/19 17:51:32

Comprehensive protein extraction from Cryphonectria parasitica is a key step to achieve high quality protein separation in two dimensional electrophoresis(2-DE). Two routine cellular total protein extraction methods were compared in order to determine an optimal one for filamentous fungi. Then the extracted total proteins were subjected to 2-DE,and the better extraction method was determined by the indexes of protein distribution and abundance on corresponding silver-stained gel. Data showed that most proteins were distributed in the pH ranging from 4 to 7; Buffer-TCA-Acetone got much more whole protein,which was treated with cleanup kit could be separated very well by 2-DE.But TCA-Acetone method precipitated total protein which is less although which shows fine differentiation among protein spots. So we consider that Buffer-TCA-Acetone is appropriate protocol for C.parasitica. Several silver -stained protein spots were picked for MALDI-TOF MS/MS to identify and high-quality PMF and MS/MS spectras were obtained. Buffer-TCA-Acetone is suitable sample preparation protocol for C.parasitica proteomics.

Tetraploid induction of in vitro Taxus chinensis cell

2016/1/19 17:51:32

Large-scale culture of Taxus cell is considered to be one of the most promising alternative methods to produce taxol. Heavy producing cell line can cut down the cost of taxol production and promote the process of industrialization. Compared with diploid,multiploid medicinal plant has the characteristics of giantism,which express in roots,stems,leaves,flowers and fruits,strong antireversion force and high contents of secondary metabolites. This research established tetraploid T.chinensis cell line by the methods of synchronization culture and inducing treatment of colchicine. Through 7 cycles subcultures,stability was demonstrated. Results showed that treating the T.chinensis cells with 750 mg/L colchicine could get stable tetraploid cell line whose tetraploid cell rate maintained in 62.5%. Further to detect taxol,its secondary metabolite,tetraploid cell line had stronger ability to produce taxol compared with diploid cell line.

Acclimation of photosynthesis and growth to different growth light conditions for seedlings of Cyclobalanopsis glaucoides

2016/1/19 17:51:32

The response of net photosynthetic rate(Pn)to photon flux density(PFD)and the growth condition for seedlings of Cyclobalanopsis glaucoides grown under three irradiance levels(80%,20% and 2% full-light)were examined in this paper. The results showed that,there was no significant differences of the light saturation point(LSP)and the Max. net photosynthetic rate among three irradiance levels,the light compensation point(LCP)in seedlings grown under 20% and 2% full-light were decreased significantly compared with 80%. The LSP were all about 400～450 μmol·m^-2·s^-1 and the LCP were bout 6～10 μmol·m^-2·s^-1 in seedlings grown in three light levels,exhibited a characteristic of shade plant. Seedlings of C.glaucoides had a strong ability of shade-tolerance,but this adaptability is at the cost of its'reduction of growth rate and dry mass accumulation. The leaf mass per unit area(LMA)decreased and the chlorophyll content increased significantly in seedlings grown under 2% full-light,showed a great capability to taking use of high irradiance,they could grow quickly when canopy formed.

Effects of the low latitude and high elevation region's UV-B radiation on the content of MDA and soluble protein of the Primula

2016/1/19 17:51:32

Utilize the UV-B radiation strength datum of five-test place in Yunnan province,which located in different latitude and different elevation but similarly in the same longitude. At the same time plant primula as experimental material,then determine the content of MDA and soluble protein in plant leaves,analysis the effects of the low latitude and high elevation region's UV-B radiation on the content of MDA and soluble protein. The results shows that from February to May the UV-B radiation strength was increased,the maximum appeared in Lijiang(12.90 w·m^-2 in May); the low latitude and high elevation region's UV-B radiation was much higher than high latitude and low elevation region's; the primula leaves content of MDA was much higher during Match and April,but the higher content of soluble protein appeared in February and April. The result shows that:with the increase of the UV-B radiation strength,the content on MDA increased at first and then; in higher UV-B radiation region the content of MDA was also higher; during different physiology time the content of MDA showing difference,the higher content emerge in flowering and fruiting duration; but with the variation of UV-B radiation strength the change of the soluble protein showing diversity in test place; during different physiology time the content of soluble protein showing variant,the higher content emerge in flowering period.

Chemical constituents of the volatile oil from Peristrophe baphica

XIE Yun-Chang, JIANG Xiao-Hua, ZHANG Mian — 2016/1/19 17:51:32

The volatile oil of Peristrophe baphica,obtained by hydrodistillation from the aerial part of the herb grown at Yizhou of Guangxi in China,were analysed by coupled gas chromatography-mass spectrometry(GC-MS). A total of 31 compounds were identified,which comprising 99.42% of the oil. The major components are:coumarin(53.66%),coumaran(9.18%),1-octen-3-ol(10.00%),trans-3-hexen-1-ol(5.85%),3-octanol(3.86%),benzyl alcohol(1.69%),linalool(1.22%),o-tolualdehyde(5.37%),p-vinylguaiacol(3.96%).

Chemical constituents in Blumea balsamifera(Ⅰ)

2016/1/19 17:51:32

Three constituents were isolated from Blumea balsamifera,and their structures were elucidated by spectroscopic evidence. They were xanthoxylin(Ⅰ),blumeatin(Ⅱ)and dihydroquercetin-7,4'-dimethylether(Ⅲ). Compound Ⅰ is obtained from genus Blumea for the first time.

Phylogenetic relationship of ramie and its wild relatives based on SRAP markers

2016/1/15 3:05:32

It was considered that there was disputation on the systematics of the genus Boehmeria,as well as the relationship among different sections in this genus. The phylogenetic relationship of 17 species and 9 varieties of Boehmeria was investigated using SRAP analysis based on a total of 27 samples. 368 bands were produced by SRAP technique using 26 primer pairs,of which 363(98.64%)bands were polymorphic.It was also indicated by clustering analysis that:(1)Boehmeria malabarica var.malabarica(sect.Boehmeria),B.nivea(sect.Tilocnide)and B.nivea var.nipononivea(sect.Tilocnide)were clustered into one branch and near the root of the phylogenetic tree,indicating that sect.Tilocnide originated from sect.Boehmeria;(2)B.nivea and B.nivea var.nipononivea were clustered into one branch,indicating that B.nivea originated directly from B.nivea var.nipononivea;(3)B.nivea var.viridula(sect.Tilocnide)and B.nivea var.tenacissima(sect.Tilocnide)were clustered together with the polyploid of sect.Duretia,which might have resulted from interspecific hybridization.

Maximum likelihood, Bayesian inference and likelihood ratio tests in the molecular phylogeny of Alismatales

2016/1/15 3:05:32

Maximum likelihood,Bayesian inference,Neighbor Joining and Hierarchical likelihood ratio tests were employed to study the phylogeny of Alismatales. 52 rbcL sequences were used in the analysis to represent 14 families 46 genera,and 6 related genera as outgroup. Hierarchical likelihood ratio tests showed that GTR+I+G could be the best evolutionary model for rbcL dataset,and the topology of maximum likelihood tree was similar to those of Bayesian tree and Neighbor Joining tree,but Bayesian tree giving higher support values; Alismatales could be composed of two major clades and further divided into five clades. The phylogenetic relationship of “families of Alismatales”,“Hydrocharitaceae + Najadaceae”,“Alismataceae + Butomataceae + Limnocharitaceae”,and “Cymodoeaceae complex” were discussed based on the molecular phylogenetic trees. The result also showed that more researches were needed to explain phylogeny of Alismatales.

Micro-structure and ultra-structure of the lipids during the seed development in Jatropha curcas

2016/1/15 3:05:32

The micro-structure and ultra-structure of the lipids during the seed development in Jatropha curcas were studied by paraffin sectioning,semi-thin sectioning and ultra-thin sectioning. The results showed that the lipids were mainly stored in endosperm. The endosperm was rich in lipids at maturity. Seed coats were abundant in lipids during seed development. The outer coat was sclerified and the inner coat was rich in lipids at maturity. The embryo had a few lipids at maturity. Organells including endoplasmic reticulum,mitochondria,plastids and dictyosome were abundant in the cell when lipids were rich. It was the most appropriate time to harvest and process when the seeds were fully matured.

Zoning of sugarcane stem tip protomeristem

2016/1/15 3:05:32

All organization of sugarcane stem and leaf origined from stem tip protomeristem. The stem tip protomeristem of six sugarcane cuctivars at four different developing stages was investigated using microstructural and ultrastructural techniques. The results showed that sugarcane stem tip protomeristem had four zones:tunica-primary cell zone,corpus-primary cell zone,peripheral meristem zone and pith zone. The tunica-primary cell zone had only one layer of tunica cells and many of plasmodesmus crossed between the tunica-primary cells,but few plasmodesmus crossed between the tunica-primary cells and the corpus-primary cells. Most of tunica-primary cell were anticlinal division in order to enlarge protomeristem superficial area. The corpus zone located in the central region of the meristem below the tunica,where the cell divisions were in all directions to increase both the width and length of the apex. The corpus-primary cell zone appeared to be a sphere. The peripheral meristem zone located the two sides under the tunica-primary and the corpus,which cells were very active and generate phyllopodium and protocambium cells. The pith zone was at the inner side of peripheral meristem under the corpus,and the cell division was transverse and end-to-end arrangement to elongate the stem.

Some new combinations in the Chinese Chrysanthemum

2016/1/15 3:05:32

Plants of the genus Chrysanthemum are valuable in horticulture and traditional Chinese Medicine. The application of the their generic name,however was confusing. This condition was partly changed recently. In this paper,new combinations are provided for three taxa in the Chinese Chrysanthemum; a list of all the Chinese native species of Chrysanthemum and their geographical distribution are provided.

New records of mosses growing on bones in China

2016/1/15 3:05:32

Four acrocarpous moss species growing on the bones of Bos grunniens L. were collected in alpine meadow of Mabian Dafengding National Nature Reserve,Sichuan,China. They were Bryum argenteum Hedw.of Bryaceae,Bryum lonchocaulon C.Muell.of Bryaceae,Weisia semipallida C.Muell.of Pottiaceae,Funaria sinensis Dix. of Funariaceae. This is the first report of the bryophytes growing on the bones in China.

Additions to the bryophyte flora of Guangdong, China

2016/1/15 3:05:32

A series of extensive field investigations were carried out in Nanling National Nature Reserve,Guangdong from August 2008 to July 2009.Thirty moss species and three genera are reported as new records from Guangdong. The newly recorded genera are Sakuria,Rhabdoweisia and Ceratodon.

A new species, Fordiophyton damingshanense (Melastomataceae)from Guangxi

2016/1/15 3:05:32

This paper describes a new species,Fordiophyton danmingshanense,from Guangxi. The new species is similar to F.faberi Stapf from Guangxi,Fujian,etc.,but differs in its leaves nerves more 7-9; pleiochasium 2.5-3 long,6-7 cm wide; longer stamens ca.2.2 cm long,anthers linear,1.1-1.3 cm long,base lengthened,slightly enlargement and forming a vesicle,free,connective enlargement,lengthened and forming a short spur,shorter stamens 1.4-1.6 cm long,anthers linear,ca.4 mm long,connective enlargement,lengthened and forming a short spur.

New records of Phalaenopsis(Orchidaceae) from Guangxi, China

2016/1/15 3:05:32

Phalaenopsis lobbii(Rchb.f.)H.R.Sweet,a newly recorded species of Orchidaceae from China. P.mannii Rchb.f.is reported as a new record to Guangxi. According to the two kinds of the current resources of Phalaenopsis, the conservation policy was put forward.

Interspecific association analysis of dominant species in shrub layer of Armandii community in Xiaolongshan Mountain of Gansu Province

2016/1/15 3:05:32

Through lots of field investigations in Xiaolongshan Mountain of Gansu Province,researchers selected 20 common species in 55 quadrates by important value in Armandi communities. In order to analyze the 20 common species interspecific association,researchers took some analysis methods,including multi-species complex associated index(VR),χ²-test; Relevance measure,the Pearson correlation coefficient,as well as the Spearman rank correlation coefficient. The results were as follows:(1)Multi-species relevance(VR)analysis showed that the association between the main components members in Armandii communities was positively correlated,this demonstrated the natures of Armandii communities and the characteristics of their living environment were adaptable;(2)In pairs of the 20 common species,the pairs of Armandii-Quercus alioena,Armandii-Quercus variabilis,Armandii-Quercus liaotungensis etc.were positively correlated; Quercus alioena-Quercus variabilis,Quercus alioena-Rhus chinensis etc.were negatively correlated; but the independence of Smilax scobinicaulis,Cerasus polytricha etc.demonstrated the different nature of communities,that was a performance of the difference of environment within community,and was cased by environmental heterogeneity within Armandii communities.(3)Armandii communities based on the different nature of the relationship between the compositions of communities in 20 common species were grouped into three ecological species groups,they are positively correlated group,the negative association group and independent group.(4)The results of the study on interspecific association had a certain practical guide of Armandii community habitat monitoring,forest management,and recycling of forest roads in the landscape species selection and configuration.

Comparison of phenology among tropical plant populations from different source regions

2016/1/15 3:05:32

Some species usually cover wide regions with different circumstances,so it was expected that the species would demonstrate various phenotypes due to different selection pressures. In this study,the phenologcial differences of 2 tropical species,e.g.,Samanea saman and Glirdia sepium planted in Xishuangbanna Tropical Botanical Garden were explored. Each species has individuals form two or three different continents and shared a same phenological observation period. Although all the plant individuals showed similar phenological pattern,there were still obvious differentiation in phenology among different populations. The synchronous index of phenology for the two species was low,and the flushing commencement dates were significantly different among the populations. Relationship analysis showed that the differentiation in phenology was related to climatic variables,especially those in the dry season. The results suggested that the different climate characters in primary habitats have resulted in phenological variation in the two species,which would influence their response to climate change in the future.

Age estimation and age structure of Osmunda japonica population

2016/1/15 3:05:32

Based on the structural characteristics of Osmunda japonica,including the length of subterranean rhizoid,vestige of leaves,diameter of basilar petiolar,a method for age estimation of O.japonica population in Guangxi Yuecheng Ling Region was developed,and the age of each individual was calculated. The approaches of age structure diagram were used to study the age structure of the population. Results revealed that age estimation of O.japonica would focus on its subterranean rhizoid,as its overground part was stable after reaching fixed age; age estimation of ferns would referring to their length of subterranean rhizoid,for they are lack of vascular cambium. O.japonica population in Guangxi Yuecheng Ling Region is aging,Natural regeneration and population structure of O.japonica are tremendously destroyed by excess picking and transplanting.

Influence of plant diversity by Amomum tsao-ko(Zingiberaceae)plantation

2016/1/15 3:05:32

Species number,individual number,coverage of trees and biodiversity index of plants in forest planting with Amomum tsao-ko and natural forest were investigated and compared. The result showed that:(1)the coverage of overstorey canopy(including arbor and shrub crown)was fluctuated between 20.0 and 50.0% in different growing phases of A.tsao-ko plantation;(2)with aging A.tsao-ko plantation,individual number of arbors and shrubs was decreased gradually,the species number also inclined to single,Alnus nepalensis was maintained as a great shading species in the A.tsao-ko plantation;(3)the herbaceous species in the natural forest was 13,and then decreased into 8 when the plantation time arrived 80 years; individual number of herbaceous plants was varied between 48 and 285 after the plantation of A.tsao-ko exceeded 5a,which was lower than the number in the beginning time of A.tsao-ko plantation;(4)the Shannon-Wiener index of arbors and shrubs was reduced to 0 at the plantation time of 30a,the Simpson index of herbaceous species was varied 0.2266-0.3399 between the plantation time from 0.5a to 1a. Although this index became stable at 0.4351-0.8062 after 5a plantation,they were all lower than the natural forest(0.7681). Therefore,in order to maintain the sustainability and stability of natural forests,A.tsao-ko was suggested to plant under the artificial forests.

Study on moss flora of Maoershan Nature Reserve, Guangxi

2016/1/15 3:05:32

Moss flora of Maoershan Nature Reserve in Guangxi Zhuang Autonomous Region is reported for the first time,based on the specimens collected through field survey and careful identification. There are 265 species and infraspecific taxa belonging to 109 genera of 39 families in Maoershan Nature Reserve,among which 6 genera and 47 species are new records to Guangxi. The moss flora of Maoershan Nature Reserve is characterized by subtropical elements. The total elements are ahead by East Asia type representing 36.06% of the entire species,followed by Tropical Asia element and North Temperate element. Gleason Index on biodiversity of mosses of Maoershan Nature Reserve is lower than that of Emei Mountains and Daweishan Nature Reserve. The values of Kroeber index on similarity of genera and species between Maoershan Nature Reserve and Daweishan Nature Reserve are higher than that between Maoershan Nature Reserve and Emei Mountains. The floristic spectrum of Maoershan Nature Reserve shows the highest percentages of elements of East Asia type and Sino-Japan sub-type,comparing with that of the other two mountains.

Effect of CaCl₂ on photosynthetic characteristic of Spinacia oleracea under enhanced UV-B radiation

2016/1/15 3:05:32

In order to reveal the effect of CaCl₂ on photosynthetic characteristic of Spinacia oleracea under the enhanced UV-B radiation,the photosynthetic pigments,chlorophyll fluorescence,Hill reaction activity and MDA content were determined. The results showed that the treatments could decrease the content of chlorophyll a,chlorophyll b,total chlorophyll,potential activity of PSII(Fv/Fo),photochemical quenching(qP)and non-photochemical quenching(qN),light quantum yield of PSII(ΦPSⅡ),efficiency of primary conversion of light energy(Fv/Fm)on thylakoid membrane,and the activity of Hill reaction,whereas,the treatments could increase chl/car,and the content of MDA. The damage of spinach leaves caused by UV-B was relieved by CaCl₂.With different treatments the change of the activity of SOD,POD,CAT was compensatory effect.The relationship between the UV-B intensity and the damaged degree of PSII function of spinach leaves was positive correlation. It appeared that the most primary action by CaCl₂ radiation occured at the chlb content in total photosyntheic pigments,the light quantum yield and the activity of POD.Under the high UV-B stress,CaCl₂ caused the chlorophyll b increase,which was the most important reason that efficiency of PSII capture light could raise.

RAPD mapping of a brown planthopper(Nilaparvata lugens)resistance gene from Oryza officinalis

2016/1/15 3:05:32

B₃F₄,a near-isogenic line(NIL)with Bph,was screened by distant hybridization of Oryza officinals 1665 and Oryza sativa Gui99,three molecular markers,S229₇₀₃,S403₇₈₃ and S1159₁₄₀₈,linked to Bph,were obtained through RAPD analysis of B₃F₄ with random primers. The result of MAPMAKER/EXP. Version 3.0 analysis showed that these three molecular markers were belonged to the same linkage group with Bph,the covered range was 18.1cM and the average distance between markers was 4.53cM. This result will facillitate the futher accurate localization and cloning the Bph gene.

Isolation and identification of antagonistic bacteria and their mechanisms against Phytophthora parasitica var.nicotianae in karst area of Guangxi

2016/1/15 3:05:32

Phytophthora parasitica var.nicotianae and soil bacteria were isolated from Jingxi county,a karst area in southwest of Guangxi province where produced high grade tabacco. Antagonistic bacteria to P.parasitica var.nicotianae were screened out according to their antagonistic action and anti-disease in vitro and field experiment. The well antagonistic effect strains were identified by combining the results of morphological observation,physiological and biochemical characteristics analyse,and 16S rDNA sequence test. And pilot study of the mechanism of anti-disease of P.parasitica var.nicotianae was carried out at the same time. Results showed that a total of 340 strains of bacteria were isolated from 8 samples of soil. And three strians with well antagonistic effect which marked as 8-23,6-70,and 13 were screened out. They were the bacteria belonged to Bacillus,Lysobacter,and Pseudomonas respectively. The mechanism of the three strains against P.parasitica var.nicotianae was to excrete some protein enzymes or other substance which could dissolve the cell wall or membrane of pathogeny and killed them finally. The active substance of strain 8-23 and 13 were mainly protein. But for the strain 6-70,there were some other factors to work besides the protein compounds.

Purification and enzymatic characterization of phospholipase A₂ from latex of Hevea brasiliensis

2016/1/15 3:05:32

A phospholipase A₂(PLA₂)was purified from the latex C-serum of Hevea brasiliensis by acetone precipitation,DEAE-Cellulose ion exchange chromatography,Sephadex G-100 gel filtration. SDS-PAGE was used to determine the Mr of the subunit of PLA₂. Then the K_m,V_max,the optimum temperature and pH of PLA₂ were tested. The effect of Ca²⁺ and La³⁺ on PLA₂ activity were assayed. A 49.47-fold purification was obtained with the recovery of 5.12% activity. The results showed that a single band on SDS-PAGE with a subunit Mr of 43 kDa. Latex C-serum PLA₂ had an optimum temperature at 37 ℃,and an optimum pH at 8.2. The K_m was 0.44 mmol·L^-1. The V_max was 7.22 μmol·(mL·min)^-1. The Ca²⁺ ion optimum concentration for activity was determined to be 50 μmol·L^-1. The activity of PLA₂ was inhibited by La³⁺. The inhibited effect of La³⁺ was palliated after added calcium ion. There are differences of Ca²⁺ dependence between latex C-serum and other plant PLA₂. The results provide a research basis for catalytic mechanism,regulation,and physiology function of PLA₂ in latex.

Breeding of superior Siraitia grosvenorii cultivars producing glycosides-Ⅴ

2016/1/15 3:05:32

To breed superior cultivars of Siraitia grosvenorii producing glycosides-V,6 important characters including glycosides-Ⅴ content,yields,fruit pulp content,water soluble matter content,total sugar content and total glycosides content of 19 kinds S.grosvenorii cultivars were analyzed and assessed comprehensively. As a result,the comprehensive indexs of F018,F014 and F020 are higher. So three superior cultivars of S.grosvenorii producing glycosides-V,which can lay the foundation for the studies on breeding of new S.grosvenorii cultivars.

Chemical constituents from the leaf of Helicia tsaii

2016/1/15 3:05:32

In order to search for the bioactive leading compounds from Helicia tsaii,six compounds were isolated from the leaf of this plant.They were identified as arbutin(1),robustaside B(2),robustaside(3),β-sitosterol(4),helicide(STHZ 5)and(E)-methyl 3-(4-hydroxyphenyl)acrylate(6).All the compounds were isolated from this plant for the first time.

Chemical constituents of Picria fel-terrae

2016/1/15 3:05:32

The chemical constituents of traditional medicinal plant Picria fel-terrae were studied,the compounds were isolated by column chromatographys and the structures of the isolated compounds were elucidated by means of spectral analysis. Seven compounds were isolated and identified as apigenin(1),apigenin-7-O-β-D-glucuronide(2),apigenin-7-O-α-L- rhamnopyranosyl(1→2)-β-D-glucuronide(3),rosmarinic acid(4),picfeltarraegenin Ⅳ(5),picfeltarraegenin X(6),and acteoside(7). The compounds 2,3 and 4 were obtained from this plant for the first time.

Determination of two nor- triterpenoid glycosides in the roots of Siraitia grosvenorii by HPLC method

2016/1/15 3:05:32

A HPLC method was developed for determining the contents of Siraitic acid ⅡB and Siraitic acid ⅡA,which were novel 29-norcucurbitane triterepene glycosides in the roots of Siraitia grosvenorii. The chromatographic analysis was carried out on a ZORBAX SB-C₁₈ column(4.6 mm×150 mm,5 μm),and the mobile phase was acetonitrile(A)-water(gradient elution:0→4 min:0→35%A,4→16 min:35%→ 45%A). The flow rate was maintained at 0.5 mL/min. The detection wavelength was set at 230 nm,and the column temperature was controlled at 25 ℃. The sample injection volume was 10 μL. The results indicated that Siraitic acid ⅡB had a linear relationship at the range of 0.05-0.3 mg/mL,and Siraitic acid ⅡA had a linear relationship at the range of 0.05-0.25 mg/mL. This method was accurate,stable and reproducible,while the preparation of samples was simple and detected time was short.

Analysis of volatile constituents of different types of Eucalyptus leaf by GC-MS

2016/1/15 3:05:32

The volatile constituents from the leaves of different Eucalyptus species were analyzed by gas chromatography-mass spectrometry(GC-MS). They were extracted by steam distillation and identified by GC-MS method. In the identified components,Eucalyptol and p-menth-1-en-8-ol are common in the leaves of these five Eucalyptus species:Eucalyptus exserta,E.urophylla&#215;E.grandis,E.pellita,E.urophylla,E.robsta. Rhodinal and critronelly acetate are the main components in the leaves of E.citriodora. The results indicated that volatile constituents and their relative content in the leaves of different Eucalyptus species are different.

Information sharing and integration of plant specimens——a case in IBK

2016/1/15 3:05:33

This article took plant specimen resources sharing and the conformity as a master line,seeked and explored the reason of“the information isolated island”phenomenon,further manifested the value of sharing conformity. Through thorough investigation and research,the authors discovered the existing questions and the causes,realized the highly effective use of plant specimen information resources by adopting the nimble diverse conformity way and the sharing pattern.

^Consideration and preliminary discussion on the internationalization development of the sci-tech academic journal

2016/1/15 3:05:33

Combining with the journal running practice,the following measures and suggestions were proposed:(1)clarifying reader positioning and building up new channels for excellent manuscripts;(2)fully embodying the innovation of knowledge by topic scheming,setting special columns,stuffing journal columns with science and practicability,optimizing the journal content by some approaches such as adding more informations and raising the information density in the avialable papers so as to construct a special journal;(3)winning more excellent manuscripts by the enhancement of policy orientation and the establishment of a reasonable mechanism,tracking the international forefront of sci-tech researches and mastering the development trends of academic teams,improving the academic quality of manuscripts by soliciting contributions on academic conferences and emphasizing the connection with introduced/dispatched visiting scholars in order to enhance the core comparativeness of the journal;(4)exchanging the publications with international editorial offices,raising the self-value of the journal;(5)closely connecting with main international retrieval systems,joining into some expertise database;(6)expediting the informationization and promoting the external publicity of the journal. Attributing to these countermeasures,the international impact of the journal is uplifted and the internationalization is enhanced.

eview on thirty-year-development of Guihaia

2016/1/15 3:05:33

This paper introduces the founding and publishing situation of Guihaia,and carries on the review analysis to 30-year-development from eight aspects,e.g.the obtaining of identification codes; the publication objective and the policy,the specialized labor division scopes,the readers; the rewarded,the collection,kinds of database abstracted and entered; statistical result of indexes; the publication of new plant taxa; international distribution and exchange; publication and talent cultivation; academic influence and so on. The results show that:Guihaia made obvious progress in all aspects for 30 years,with great unceasing improvement of publishing quality,and made proper contribution to discovering and fostering talents in the field of botany. Looking forward to the future,under the coexistence of difficulty,challenge, opportunity and hope,the editorial department should continuously aim at improving the journal quality and realizing the journal digitalization and network,integrate the journal in the big circulation of world academic information and finally exhibit it to the world.

Effects of phosphogypsum extracts on seed physiology and seedlings growth of pea

2016/1/20 14:07:29

The activities of amylase and indole acetic acid oxidase,soluble sugar,seed vitality,indole acetic acid in pea seed and germination rate,seedlings height and fresh weight were examined after the treatment of pea seed using different concentrations of aqueous phosphogypsum extracts. The results showed that the contents of soluble sugar and indole acid in the pea seed were increased by 6.7%-43.3% and 9.4%-40.8% respectively compared to the control. The activities of α-amylase and IAA oxidase were 8%-64% and 15.2%-30.9% higher than CK. Germination rate,seedlings height and fresh weight of the pea were 10% more than CK. This indicated that phosphogypsum could promote the pea seed vitality and germination.

Determination of huperzine A,B and C in Huperzia serrata from western Hunan by HPLC

2016/1/20 14:07:29

For determining the contents of huperzine A,B and C in the whole herb and different parts of Huperzia serrata from western Hunan,total alkaloids of samples were extracted by ultrasound with 2% tartaric acid and then analyzed by HPLC,in which chromatographic column was Diamonsil C₁₈(250 mm×4.6 mm,5 μm),mobile phase was methanol-0.08 mol/L CH₃COONH₄,flow speed 1 mL/min,column temperature 25 ℃ and detection wavelength 308 nm. The results showed that the contents of huperzine A,huperzine B and huperzine C in H. serrata from 4 sample plots were similar,and up to 0.5‰,0.3‰ and 0.04‰ respectively. The contents of huperzine A,B and C in the different parts of H. serrata had significant differences,in which the distribution of huperzine A and B was rootstem and leaf. In conclusion,there are superior resources of H. serrata for huperzine production in western Hunan,the distribution of huperzine in different parts of H. serrata had high selectivity,and the HPLC method to measure three kinds of huperzine simultaneously was simple,accurate and reliable.

Basic biology characteristics and feeding value of Vigna minina ——Comparative study with V. angularis and V. radiata

2016/1/20 14:07:29

In order to know the basic biology characteristics and its feeding value of Vigna minina,a comparative study with V. angularis and V. radiata were carried out, especially the V. minina's wild growth situation, germination rate,traits of germination,traits of anti-intimidating and the methods of breaking its dormancy and so on were all studied in laboratory. At the same time,the nutrient ingredients of V. minina's seed and young seedling were measured. The results were as follows:V. minina's vitality was very strong in wild,growth periods was long,more pods were produced,if its seedling came out later,the moment of procreation was earlier. Under the experimental conditions,V. minina's germination rate was very low without order; in the period of germination,V. minina put up the ability of enduring the feebleness acid and was fond of the stimulation of low salinity; using 98% oil of vitriol to manage the seed of V. minina 30 min and using knife to lacerate the seed capsule were the best method to break its dormancy. The V. minina's coarse fat and solubility sugar were more than that of V. angularis and V. radiata,but its coarse protein was a bit low. In young seedling,solubility sugar in V. minina was the most,solubility protein in V. minina was more than V. radiata,lower than V. angularis,the dissociative amino acid in V. minina was the lowest.Comparing with the general feedstuff,like corn,barley,ryegrass and alfalfa,the V. minina had more evidence predominance. In conclusion, serving as crop of feedstuff,aiming at V. minina's exploitation and using would have better foreground.

Inter-sexual difference of growth characteristic for Populus cathayana natural forests in the Xiaowutai Mountains

2016/1/20 14:07:29

It was investigated that whether the gender differences existed in sexual maturation conditions and morphological characteristics for this dioecious plant,by the field research for the natural population of Populus cathayana in the Xiaowutai Mountains,and using the allometric models for DBH(diameter at breast height)and plant height to analyze the growth characteristics between the male and female groups. The results were as follows:(1)In the growth process of Populus cathayana,the DBH increased exponentially with the growth of the age,while its plant height was logarithmic growth;(2)Sexual maturation conditions were different between male and female plants. The minimum age of sexual maturity stage and the DBH for female plants were both less than males;(3)The gender differences presented in the growth process. The allometric exponent for female plants was significantly higher than males(P=0.024). It was shown that female plants matured generally earlier in the Populus cathayana natural population,and the vegetative growth emphasized mainly thickening of DBH; while male plants matured later,and emphasized plant height. Compared with the male plants,the female ones had a higher trunk mechanical strength.

Succession analysis of forest communities in Wulu Mountain National Nature Reserve

2016/1/20 14:07:29

Succession analysis of plant community has great significance on vegetation restoration and forest management. It was analyzed by the method of space instead of time,collecting data and using the quantitative classification method(TWINSPAN)and the ordination technique(DCA). The results of the succession series of forest communities in Wulu Mountain were as follows:form. Zizyphus jujube var. spinosa→form. Vitex negundo var. heteophylla→form. Rosa xanthina→form. Pinus bungeana+Platycladus orientalis→form. Pinus bungeana→form. Pinus tabulaeformis→form. Quercus liaotungensis; form. Ostryopsis davidiana→form. Acer ginnala→form. Larix principis-rupprechtii→form. Betla platyphylla. That could be used as plant community succession model of Wulu Mountain. Species structure,composition and life-forms changed significantly in the succession process. The succession of species heterogeneity,evenness and richness change were analyzed with six indexes. As succession proceeds,species heterogeneity and richness are increasing,but the evenness is reducing gradually.

Antimicrobial activity of the extracts from Citrullus vulgaris Schrad vine

2016/1/20 14:07:29

Agar dilution method was applied to detect the antimicrobial effects of Citrullus vulgaris Schrad vine extract in vitro,and the mice celiac injection of staphylococcus aureus method was applied to detect the antimicrobial effect of C. vulgaris Schrad of 80% alcohol extract in vitro,in order to study antimicrobial activity in vitro of C. vulgaris Schrad vine extract. C. vulgaris Schrad vine extract had different inhibition effects on staphylococcus aureus,escherichia coli,bacillus aeruginosus,salmonella typh,bacillus subtilis and klebsiella pneumoniae,but it showed weak inhibition effect on chain coccus. Effects of 80% alcohol extract and ethyl acetate extract were better than others on staphylococcus aureus. The minimum inhibition concentration(MIC),was 4.2 mg·mL^-1,8.4 mg·mL^-1 respectively. C. vulgaris Schrad vine extract had antibacterial action,and would provide a basis to exploit new antimicro-bic drug.

New records of naturalized plants from the Chinese Mainland

2016/1/20 14:07:29

Anthriscus caucalis M. Bieb. and Tagetes minuta L. are reported as the newly naturalized plants of Chinese Mainland. Their morphological characters,photographs and specimens are also provided for identification.

Effect study on a biological prevention method applying mix-culturing of saltmarsh and Avicennia marina mangrove trees to reduce marine fouling fauna damage

2015/12/15 11:20:39

In Beilun estuarine area of Guangxi,China,the intertidal flat were filled to an elevation of about 220 cm Yellow Sea Datum to mix-culture Avicennia marina(AM)seedlings with four species of saltmarsh,aimed at studying the effect of biological prevention method for reducing the fouling damage on mangrove trees. The results showed that Cyperus malaccensis(CM)and Zoysia matrella(ZM)were able to grow and spread faster,and more efficient to slow down water velocity and accelerate sedimentation than Scirpus validus var. laeviglumis(SV)and Phragmites australis(PA). 19 species of fouling fauna were found on saltmarsh and mangroves,among which Balanus littoralis,Euraphia withersi,Littorina melanostoma and L. scabra were major dominant species. Among four saltmarsh species,the fouled degrees on CM and ZM were significantly less than those on other two species. And the fouled degrees on four saltmarsh species were all significantly less than that on A. marina tree. The mix-culturing of saltmarsh CM or ZM with mangrove tree AM could efficiently reduce the fouling damage,resulting in better tree growth indexes i.e. tree height,leaf number,branch number,branch conservation and survival rate than those in mono-species treatment areas. In general,a saltmarsh-mangrove cooperating ecological conservation system could be a practical model for mangrove afforestation. In Beilun estuarine area of Guangxi,China,the intertidal flat were filled to an elevation of about 220 cm Yellow Sea Datum to mix-culture Avicennia marina(AM)seedlings with four species of saltmarsh,aimed at studying the effect of biological prevention method for reducing the fouling damage on mangrove trees. The results showed that Cyperus malaccensis(CM)and Zoysia matrella(ZM)were able to grow and spread faster,and more efficient to slow down water velocity and accelerate sedimentation than Scirpus validus var. laeviglumis(SV)and Phragmites australis(PA). 19 species of fouling fauna were found on saltmarsh and mangroves,among which Balanus littoralis,Euraphia withersi,Littorina melanostoma and L. scabra were major dominant species. Among four saltmarsh species,the fouled degrees on CM and ZM were significantly less than those on other two species. And the fouled degrees on four saltmarsh species were all significantly less than that on A. marina tree. The mix-culturing of saltmarsh CM or ZM with mangrove tree AM could efficiently reduce the fouling damage,resulting in better tree growth indexes i.e. tree height,leaf number,branch number,branch conservation and survival rate than those in mono-species treatment areas. In general,a saltmarsh-mangrove cooperating ecological conservation system could be a practical model for mangrove afforestation.

Monitoring of airborne heavy metals by using moss-bags in Shenzhen

2015/12/15 11:20:39

This present study investigated airborne trace heavy metals of V,Cr,Mn,Co,Ni,Cu,Zn,As,Cd and Pb in Shenzhen by using moss-bag technique plus ICP-AES analysis. Seventeen sites across four administrative regions,including Yantian,Luohu,Futian and Nanshan were selected for placing moss bags. The sesults showed that the trace heavy metals varied in these four districts. The pollution of Nanshan was less serious than that of the other three districts. The main reasons were probably related to the land and water traffic conditions,industrial development,population density and geographic location. Cluster analysis of heavy metal concentration contents showed in all monitored sites,the most polluted ones were of industrial and traffic heavy areas,and relative mild polluted were far from industry and highways to some extent. The above showed that the industry and highways played a crucial role in atmospheric pollution. The government must do more to alleviate the atmospheric heavy metal pollution in Shenzhen.

Effect of exogenous salicylic acid on physiological characteristics of Sabina seedlings under low temperature stress

2015/12/15 11:20:39

The effect of exogenous SA on membrane lipid peroxidation,osmotic regulation substances and antioxidant enzyme activities in the leaves of Sabina species under low temperature stress at -4 ℃ for 6 d was analyzed by determining the relative water content(RWC),relative electrical conductivity(REC),contents of malondialdehyde(MDA),soluble sugar(SS),soluble protein(SP),proline(Pro)and carotenoid(Car),activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),ascorbic acid peroxidase(APX)and glutathione reductase(GR)in the leaves of 3-year-old seedlings of S. przewalskii and S. chinensis pretreated with different concentrations of SA to provide a theoretical basis for fostering more anti-freeze evergreen varieties. The results indicated that certain concentrations of SA could maintain the stability of membrane system and increase the contents of osmotic regulators and antioxidant enzyme activities,enhance freezing tolerance of plant,the optimum SA concentration was found to be 200 mg/L for S. przewalskii and 30 mg/L for S. chinensis. The chilling tolerance of S. chinensis was stronger than that of S. przewalskii in the pretreatments with SA. Those results suggested that appropriate concentration of SA could help to increase the chilling resistance of Sabina species.The effect of exogenous SA on membrane lipid peroxidation,osmotic regulation substances and antioxidant enzyme activities in the leaves of Sabina species under low temperature stress at -4 ℃ for 6 d was analyzed by determining the relative water content(RWC),relative electrical conductivity(REC),contents of malondialdehyde(MDA),soluble sugar(SS),soluble protein(SP),proline(Pro)and carotenoid(Car),activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),ascorbic acid peroxidase(APX)and glutathione reductase(GR)in the leaves of 3-year-old seedlings of S. przewalskii and S. chinensis pretreated with different concentrations of SA to provide a theoretical basis for fostering more anti-freeze evergreen varieties. The results indicated that certain concentrations of SA could maintain the stability of membrane system and increase the contents of osmotic regulators and antioxidant enzyme activities,enhance freezing tolerance of plant,the optimum SA concentration was found to be 200 mg/L for S. przewalskii and 30 mg/L for S. chinensis. The chilling tolerance of S. chinensis was stronger than that of S. przewalskii in the pretreatments with SA. Those results suggested that appropriate concentration of SA could help to increase the chilling resistance of Sabina species.

Effects of hormone types and concentrations on cutting propagation of Rhododendron latoucheae and its evaluation

2015/12/15 11:20:40

Using cuttings of annual lignified branches of Rhododendron latoucheae as the material and using sand and soil(3:1,V/V)as the substrate,the effects of hormone types and concentrations and their combinations on 7 rooting traits and 5 above ground growth traits of R. latoucheae cutting propagation were studied,adopting a completely randomized design(CRD)with two factor of 5 hormone types and 4 concentration levels. And on this basis,the effects of different treatments combinations on cutting propagation were comprehensively evaluated by principal component analysis. The results showed that:Two main factors had significant influences on most cutting propagation traits,and hormone types had greater effect than concentration levels. Among 5 hormone types,GA₃ had the best effect on callusing rate,rotting rate,rooting rate,retention rate of old leaf,retention no of old leaf,and new shoot rate. And IBA had the best effect on number of adventitious root,length of longest adventitious root,number of total roots,diameter of root system,number of new shoot,and length of new shoot. Both of GA₃ and IBA were ideal hormones for R. latoucheae cutting propagation,followed by IAA,NAA and 6-BA had worse effect on propagation traits which should not be used for R. latoucheae cutting propagation. Among 4 concentration levels,low concentration(B₁)had the best effect for callusing rate,rotting rate and retention rate of old leaf,as the concentration became higher,it was less effective. Middle concentration(B₃)had the best effect for the other 9 propagation traits,and these traits decreased when treated with high concentration(B₄). The interaction effect of hormone types and concentrations had very significant influence on number of total roots,and it had significant influence on callusing rate and diameter of root system,and the best concentration level depended on hormone type. The results of comprehensive evaluation using principal component analysis showed that 50 mg·L^-1 GA₃ was the best treatment combination,followed by 200 mg·L^-1 IBA and 100 mg·

Cloning and bioinformatics analysis of squalene synthase gene(SQS)from Hevea brasiliensis

2015/12/15 11:20:40

Squalene synthase(SQS)is a key enzyme in plant terpenoid biosynthetic pathway. The total RNA were extracted from Hevea brasiliensis relax,and the cDNA of squalene synthase was cloned using RT-PCR and RACE strategy. The results showed that the cDNA(named as HbSQS)contained a 1 239 bp open reading frame and encoded a predicted protein of 413 amino acids. SYBR Green I Real Time RT-PCR analysis indicated that the HbSQS was more highly expressed in bark than in leaf. The transcription of HbSQS in latex was induced by ethylene.

Bioinformatics analysis of NRT gene sequences in Rhododendron fortunei

2015/12/15 11:20:40

We used next generation sequencing technology to investigate the transcriptomes between inoculated roots and uninoculated roots of the Rhododendron fortunei and obtained many differentially expressed genes. In this paper,the nucleic acid sequences and amino acid sequences of nitrate transporters from R. fortunei,were analyzed by bioinformatics tools. Several parameters of these sequences,including sequences composition,physicochemical property,leader peptide,topological structure of transmembrane regions,hydrophobicity or hydrophilicity,secondary structures,functional domains and protein structures,were predicted. Phylogenetic tree was reconstructed for the nitrate transporters protein family. Provide the bioinformatics foundation to understand NRT gene’s function in inoculated seedling roots.

Gene expression analysis of five OsWRKY transcription factors induced by abiotic and rice blast stresses

2015/12/15 11:20:40

OsWRKY transcription factors have vital function in respond to adverse environment and pathogens in rice. In order to elucidate the regulatory role of these genes,we used Realtime-PCR to investigate expression profiles of five OsWRKY transcription factors(OsWRKY7,OsWRKY11,OsWRKY30,OsWRKY70 and OsWRKY89)under abiotic stresses and pathogen challenges. The expression characteristics of hormone treatments were also investigated in order to study which hormone way they were functioning through. All five OsWRKY could be induced by pathogen inoculation and all these genes had different rate inductions under abiotic stress. In each hormone treatment,some were induced,others were suppressed,and still others unaffected. Five OsWRKY genes were all likely to be involved in the infection process of rice blast stress. OsWRKY7 and OsWRKY70 might participate in the resistance to rice blast by JA and SA in a mutually antagonistical manner; OsWRKY89 also might be involved in it,but not through the four hormone ways studied in this research. In the aspect of abiotic stress,OsWRKY7 might participate in drought,high salt and extreme temperature stresses through the ABA way; OsWRKY11 had the possibility to be involved in high salt stress; OsWRKY30 was possible to participate in high salt and hot stresses; OsWRKY70 might be involved in high salt,drought and extreme temperature stresses; OsWRKY89 might be involved in hot stress,but not through the four hormone ways studied in this research.OsWRKY transcription factors have vital function in respond to adverse environment and pathogens in rice. In order to elucidate the regulatory role of these genes,we used Realtime-PCR to investigate expression profiles of five OsWRKY transcription factors(OsWRKY7,OsWRKY11,OsWRKY30,OsWRKY70 and OsWRKY89)under abiotic stresses and pathogen challenges. The expression characteristics of hormone treatments were also investigated in order to study which hormone way they were functioning through. All five OsWRKY could be induced by pathogen inoculation and all these genes had different rate inductions under abiotic stress. In each hormone treatment,some were induced,others were suppressed,and still others unaffected. Five OsWRKY genes were all likely to be involved in the infection process of rice blast stress. OsWRKY7 and OsWRKY70 might participate in the resistance to rice blast by JA and SA in a mutually antagonistical manner; OsWRKY89 also might be involved in it,but not through the four hormone ways studied in this research. In the aspect of abiotic stress,OsWRKY7 might participate in drought,high salt and extreme temperature stresses through the ABA way; OsWRKY11 had the possibility to be involved in high salt stress; OsWRKY30 was possible to participate in high salt and hot stresses; OsWRKY70 might be involved in high salt,drought and extreme temperature stresses; OsWRKY89 might be involved in hot stress,but not through the four hormone ways studied in this research.

Isolation and identify of the rice OsGSTLc promoter

2015/12/15 11:20:40

The expression of OsGSTLc in rice roots was induced by chlorsulfuron through semi-quantitative RT-PCR analysis demonstrated. A 2 171 bp upstream sequence of the translation start codon ATG of OsGSTLc gene was isolated from the genomic DNA of rice,which contained a putative CAAT-box at position -86 bp upstream of ATG,but there wasn’t TATA-box between the CAAT-box and ATG. Therefore,OsGSTLc promoter was a TATA-less promoter. To define the core promoter sequence,a series of 5’ truncation derivatives of GST2171 were fused to a GUS reporter gene to construct GSTL2171::GUS,GSTL1761::GUS,GSTL962::GUS and GSTL525::GUS,respectively. The four fusion genes were introduced into rice plants by Agrobacterium-mediated transformation,all promoter fragments with 5’-deletion drived successfully the expression of GUS report gene. Quantitative fluorescence assays showed that the GUS activities in the roots of GSTL2171::GUS,GSTL1761::GUS and GSTL962::GUS transgenic rice seedlings were upregulated by chlorsulfuron. The transcriptional activation element of chlorsulfuron may be located between positions -962 and -525.The expression of OsGSTLc in rice roots was induced by chlorsulfuron through semi-quantitative RT-PCR analysis demonstrated. A 2 171 bp upstream sequence of the translation start codon ATG of OsGSTLc gene was isolated from the genomic DNA of rice,which contained a putative CAAT-box at position -86 bp upstream of ATG,but there wasn’t TATA-box between the CAAT-box and ATG. Therefore,OsGSTLc promoter was a TATA-less promoter. To define the core promoter sequence,a series of 5’ truncation derivatives of GST2171 were fused to a GUS reporter gene to construct GSTL2171::GUS,GSTL1761::GUS,GSTL962::GUS and GSTL525::GUS,respectively. The four fusion genes were introduced into rice plants by Agrobacterium-mediated transformation,all promoter fragments with 5’-deletion drived successfully the expression of GUS report gene. Quantitative fluorescence assays showed that the GUS activities in the roots of GSTL2171::GUS,GSTL1761::GUS and GSTL962::GUS transgenic rice seedlings were upregulated by chlorsulfuron. The transcriptional activation element of chlorsulfuron may be located between positions -962 and -525.

Anatomical structures and ecological adaptability research of nine species of psammophytes on beach

2015/12/15 11:20:40

The morphology and anatomy of vegetative organs of psammophytes of 9 species on Beidaihe beach was studied using traditional paraffin section. The results were as follows:(1)The leaves showed the features of xerophil,for instance,palisade tissues were very developed,while their spongy tissues degenerated in some plants; The leaves of the most plants showed very small and has more ramificate vein;(2)The axial organs had more developed epidermis and strong mechanical tissue,and abnormal structures appeared. These results suggested that vegetative organs of psammophytes on Beidaihe beach showed a series of xeric structures. These features of structure had a positive effect on reducing transpiration,boosting up photosynthesis,saving up water,enhancing strength of organs.

The climate change and alpine grassland on the Tibetan Plateau

2015/12/15 11:20:40

The paper systematically summarized the tendency of climate change and its potential effects on alpine grassland of Tibetan Plateau nearly past 50 years. The data showed that the magnitude of temperature increase on the Tibetan plateau was significantly greater than the average level of China even the world. Although the annual total precipitation tended to increase,the spatial and temporal variations were high from Southern to Northern and from Western to Eastern of the plateau. The climate changes significantly affected the alpine grassland of the Tibetan Plateau. The results from observations and simulations indicated that:(1)The climate zones and vegetation zones of Tibet Plateau could move to northward and westward,whereas the variation tendency of different vegetation areas depended on the different scenarios simulated by different models;(2)Rising temperatures advanced green-up and delayed yellow period from observations which prolonged growing seasons,but remote sensing data showed opposite results since the end of 1990s;(3)Species richness decreased and species composition changed;(4)the vegetation coverage and NPP enhanced from simulated scenarios,but opposite results were found from OTC experiments in the region;(5)Although prolong growing season may improve plant production,the risk of natural disasters could also increase for forages and crops;(6)The future climate change may has a little effect on soil carbon source/sink through simulated different scenarios. Based on the review,we found that generally there were inconsistent results about the climate change and its potential effects on alpine grassland so far on the Tibetan plateau. Therefore,it is necessary for us to research how observed temperature increase to affect structures and functions of the alpine system,and how to bring the generated changes into global change models or evaluation systems,in order to accurately evaluate the long-term effects of climate change in the region.The paper systematically summarized the tendency of climate change and its potential effects on alpine grassland of Tibetan Plateau nearly past 50 years. The data showed that the magnitude of temperature increase on the Tibetan plateau was significantly greater than the average level of China even the world. Although the annual total precipitation tended to increase,the spatial and temporal variations were high from Southern to Northern and from Western to Eastern of the plateau. The climate changes significantly affected the alpine grassland of the Tibetan Plateau. The results from observations and simulations indicated that

Neolitsea homilantha’s tree-ring characteristics and its relationship with environment factors in Shilin karst region, Yunnan Province

2015/12/15 11:20:40

Tree-ring records changes of climate and environmental factors in the process of growth. Some plants can survive over the rocky desertification process own to their sprouting ability. Tree ring of Neolitsea homilantha in Shilin karst region. The relationship between sprouter growth and environment factors were investigated in Yunnan Province. The results were as follows: Individuals of N. homilantha was significantly different from each other; The ring width and ring index of N. homilantha had large variations between different sample trees,and the ring width and ring index were increased in association with basal diameter increases; The ring index of N. homilantha was positively correlated with precipitation of the rain season,while it was negatively correlated with precipitation of the dry season,and it was also negatively correlated with temperature of May,June,November and December. But all of the relationships were not statistically significant. These results indicated that the sprouters of N. homilantha had high capability to adapt to the rain and temperature variation,and thus tenaciously survive in the rocky desertification area.

Altitudinal variation of leaf traits of Quercus baronii in Yunqiu Mountain

2015/12/15 11:20:40

Combining field investigation and indoor experiments of Quercus baronii in Yunqiu scenic spot of south Lüliang Mountain,some representative indicators such as specific leaf area(SLA),leaf area(LA),leaf dry matter content(LDMC),leaf aspect ratio(L/W),chlorophyll content(Chl),and leaf nitrogen content were performed and analyzed. The results were as follows:(1)As elevation gradient rises up,Q. baronii aspect ratio,Chlorophyll,SLA,LA,N_area,N_mass,saturated fresh and dry weight,all of their indicators had a first-increase-and-then-decrease trend and their peak value appeared at the altitude of 1 180 m; however,LDMC had a significant negative correlation(P<0.05)with the elevation gradient;(2)The LA of Q. baronii was significantly positively correlated with SLA and N_mass(P<0.01),negtively with leaf dry matter(P<0.05),but obviously negatively with N_area(P<0.01);(3)The leaf SLA of Q. baronii had a positive correlation with leaf length-width ratio(P<0.01)and an obvious positive correlation with N_mass(P<0.01),while significantly positively correlated with LDMC and N_mass(P<0.05);(4)LDMC of Q. baronii was significantly positively correlated with N_area(P<0.05); This paper studied the correlation between leaf character factor of Q. baronii and the altitudinal as well as the most suitable growth condition for Q. baronii by observing the variation regulations of Q. baronii with different elevations in Yunqiu Mountain scenic spot. Accordingly,this result could provide a theoretical support for protecting and cultivating Q. baronii and a basis for exploiting and utilizing Q. baronii resource scientifically.

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Elatostema duyunense,a new species of Urticaceae from Guizhou,China

Pollen characteristics of twenty-eight species of Saussurea in alpine meadow of the eastern fringe of the Qinghai-tibetan Plateau

Observation on the double fertilization in rice by using confocal microscopy

Megasporogenesis and microsporogenesis as well as male and female gametophyte development of Pittosporum tobira

Spermodermal micro-morphology of Bauhinia ( Leguminosae: Caesalpinioideae )

Study on the vessel elements and tylosis of secondary xylem in Averrhoa carambola

Mosses flora of Red River in Guizhou Province

Study on floristic phytogeography of seed plants in the Pamirs of China

Effects of dwarf bamboo on the regeneration of dominant tree species in gaps of subalpine dark coniferous forest

Species diversity change pattern in gap gradient in karst forest in Maolan Nature Reserve, Guizhou Province

Preliminary study on population stucture and its dynamic development of Pentaphylax euryoides in the hilly country of Northeast Guangxi

Liana diversity and abundance of a tropical montane rainforest in Mengsong, Southern Yunnan, China

Community characteristics of Rhododendron simiarum in Shimentai Nature Reserve, Guangdong

Study on intraspecific and interspecific competition of Pinus taiwanensis

Dynamic distributes of endophytic fungi from Camellia sinensis

Phenophase observation and introduction and cultivation of ornamental ferns

Effect of STS on biological and physiological changes of the tree peony

Study on preservation in vitro of Morinda officinalis

Growth response and threshold concentration of Agrostis stolonifera to Cu2+, Zn2+,Cd2+or Pb2+ stress

Construction of pepper CaNPR1-RNAi expression vector and its transformation to pepper

Histological study on the somatic embryogenesis and development of Saposhnikovia divaricata

Study on the pathogenicity of Cryphonectria parasitica by 2-DE

Tetraploid induction of in vitro Taxus chinensis cell

Acclimation of photosynthesis and growth to different growth light conditions for seedlings of Cyclobalanopsis glaucoides

Effects of the low latitude and high elevation region's UV-B radiation on the content of MDA and soluble protein of the Primula

Chemical constituents of the volatile oil from Peristrophe baphica

Chemical constituents in Blumea balsamifera(Ⅰ)

Phylogenetic relationship of ramie and its wild relatives based on SRAP markers

Maximum likelihood, Bayesian inference and likelihood ratio tests in the molecular phylogeny of Alismatales

Micro-structure and ultra-structure of the lipids during the seed development in Jatropha curcas

Zoning of sugarcane stem tip protomeristem

Some new combinations in the Chinese Chrysanthemum

New records of mosses growing on bones in China

Additions to the bryophyte flora of Guangdong, China

A new species, Fordiophyton damingshanense (Melastomataceae)from Guangxi

New records of Phalaenopsis(Orchidaceae) from Guangxi, China

Interspecific association analysis of dominant species in shrub layer of Armandii community in Xiaolongshan Mountain of Gansu Province

Comparison of phenology among tropical plant populations from different source regions

Age estimation and age structure of Osmunda japonica population

Influence of plant diversity by Amomum tsao-ko(Zingiberaceae)plantation

Study on moss flora of Maoershan Nature Reserve, Guangxi

Effect of CaCl2 on photosynthetic characteristic of Spinacia oleracea under enhanced UV-B radiation

RAPD mapping of a brown planthopper(Nilaparvata lugens)resistance gene from Oryza officinalis

Isolation and identification of antagonistic bacteria and their mechanisms against Phytophthora parasitica var.nicotianae in karst area of Guangxi

Purification and enzymatic characterization of phospholipase A2 from latex of Hevea brasiliensis

Breeding of superior Siraitia grosvenorii cultivars producing glycosides-Ⅴ

Chemical constituents from the leaf of Helicia tsaii

Chemical constituents of Picria fel-terrae

Determination of two nor- triterpenoid glycosides in the roots of Siraitia grosvenorii by HPLC method

Analysis of volatile constituents of different types of Eucalyptus leaf by GC-MS

Information sharing and integration of plant specimens——a case in IBK

Consideration and preliminary discussion on the internationalization development of the sci-tech academic journal

eview on thirty-year-development of Guihaia

Effects of phosphogypsum extracts on seed physiology and seedlings growth of pea

Determination of huperzine A,B and C in Huperzia serrata from western Hunan by HPLC

Basic biology characteristics and feeding value of Vigna minina ——Comparative study with V. angularis and V. radiata

Inter-sexual difference of growth characteristic for Populus cathayana natural forests in the Xiaowutai Mountains

Succession analysis of forest communities in Wulu Mountain National Nature Reserve

Antimicrobial activity of the extracts from Citrullus vulgaris Schrad vine

New records of naturalized plants from the Chinese Mainland

Effect study on a biological prevention method applying mix-culturing of saltmarsh and Avicennia marina mangrove trees to reduce marine fouling fauna damage

Monitoring of airborne heavy metals by using moss-bags in Shenzhen

Effect of exogenous salicylic acid on physiological characteristics of Sabina seedlings under low temperature stress

Effects of hormone types and concentrations on cutting propagation of Rhododendron latoucheae and its evaluation

Cloning and bioinformatics analysis of squalene synthase gene(SQS)from Hevea brasiliensis

Bioinformatics analysis of NRT gene sequences in Rhododendron fortunei

Gene expression analysis of five OsWRKY transcription factors induced by abiotic and rice blast stresses

Isolation and identify of the rice OsGSTLc promoter

Anatomical structures and ecological adaptability research of nine species of psammophytes on beach

The climate change and alpine grassland on the Tibetan Plateau

Neolitsea homilantha’s tree-ring characteristics and its relationship with environment factors in Shilin karst region, Yunnan Province

Altitudinal variation of leaf traits of Quercus baronii in Yunqiu Mountain

Growth response and threshold concentration of Agrostis stolonifera to Cu²⁺, Zn²⁺,Cd²⁺or Pb²⁺ stress

Effect of CaCl₂ on photosynthetic characteristic of Spinacia oleracea under enhanced UV-B radiation

Purification and enzymatic characterization of phospholipase A₂ from latex of Hevea brasiliensis

^Consideration and preliminary discussion on the internationalization development of the sci-tech academic journal