Cycas had been established and applied to discuss the relationships among C.hainanensis,C.taiwaniana,C.guangdongensis C.diannanensis and C.fairylakea. The results showed that RAPD was affected by the concentrations of four components i.e.Mg²⁺,dNTP,Taq DNA polymerase and primers. The optimal reaction system was as follows:20 μL reaction volume,including 10 mmol/L Tris-HCl(pH 8.3),50 mmol/L KCl,2.0 mmol/L MgCl₂,200 μmol/L dNTP,0.3 μmol/L primers,50 ng template DNA,1.0 U Taq DNA polymerase. The reflection of RAPD cluster to the genetic relationship of all the species showed that RAPD was suitable for analysis of interspecific genetic relationship in Cycas. Genetic relationship analysis by RAPD markers showed that five defined species had far genetic relationships each other,combining with the analysis of morphological traits,it's concluded that they were five independent genetic units at species level.]]> 2016/1/15 21:07:57 1,5*, NONG Bao-Xuan^2,3, LIU Chi², ZHONG Xiao-Qing^4,5, WEI Li-Ping⁶]]> HUANG Yu-Yuan^1,5*, NONG Bao-Xuan^2,3, LIU Chi², ZHONG Xiao-Qing^4,5, WEI Li-Ping⁶ 27true Rhododendron seniavinii var.shangyounicum is a new variety,which belongs to the family Ericaceae and genus Rhododendron. As to make classification of this new variety more true,many evidences of ITS has been studied in this paper and the results are as follows:(1)Based on the strict consensus and most parsimonious tree of ITS sequences,Rh.seniavinii var.shangyounicum has not been clustered with Rh.seniavinii var.seniavinii;(2)G+C content at second codon positions(G+C2)is more different Rh.seniavinii var.shangyounicum and Rh.seniavinii var.seniavinii,and G+C content at coding positions(G+Cc)also is different between them;(3)morphologic characters between this two taxa is clearly different by some intermittent characters;(4)Codon Bias Index CBI shows features of a new variety Rh.seniavinii var.shangyounicum which is in evolving. According to the evidences above,it is reasonable that Rh.seniavinii var.shangyounicum should be treated as a variety.]]> 2016/1/15 21:07:57 LIU Ren-Lin, CAO Li-Min, XIAO Hong 26true Paraisometrum W.T.Wang was firstly collected from western Guangxi,this genus is similar to Isometrum Craib,but differs by having corolla with a 4-lobed upper lip and an undivided lower lip,stamens that are adnata to above the middle of the corolla tube,and the pistil with one stigma. Paraisometrum is a monotypic genus,with only one species,namely P.mileense W.T.Wang,which at present is only found in southeastern Yunnan and western Guangxi. There is only one distrbution spot in Guangxi. P.mileense is reported for the first time from Guangxi,the voucher specimens are stored in Herbarium of Guangxi Institute of Botany(IBK).]]> 2016/1/15 21:07:57 1, PAN Bo¹, HUANG Yu-Song^1,2, YE Xiao-Xia^1,2, LIU Yan^1*]]> XU Wei-Bin¹, PAN Bo¹, HUANG Yu-Song^1,2, YE Xiao-Xia^1,2, LIU Yan^1* 25true Wrightoporia Pouzar,and reported a new Chinese record of wood-rotting fungus,Wrightoporia japonica Núñez & Ryvarden. The species was collected on rotten angiosperm wood from Guangxi Zhuang Autonomous Region,and its illustrated description was given according to the Chinese material.]]> 2016/1/15 21:07:57 1,2, YUAN Hai-Sheng¹, DAI Yu-Cheng^3*]]> ZHOU Xu-Shen^1,2, YUAN Hai-Sheng¹, DAI Yu-Cheng^3* 24true Lycium barbarum were studied by using TTC,benzidine-hydrogen peroxide,pollen-ovule ratio,out-crossing index and bagging experiment. The results showed that:the ratio of pollen quantity and ovule of L.barbarum is more than 4 000,and hybrid index is between 3 and 4. The combination of artificial pollination and bagging experiments determined that among these 13 participants,Maye(Ningqi 1,Ningqi 2,Damaye,Xiaomaye)performanced partly as self-compatible,while other materials(Baihua,Baitiao,Ningqi 3,Jiantouyuanguo,Yuantouyuanguo,Mengqi 1,Bianguo,0701 and 0616 )showed self-incompatible,flower with different strains,different strains of different flower hardly setting,and under natural conditions only through mixing with other materials can they achieve replanting seed. Because of the extremely low level self-compatibility,the breeding system of L.barbarum should belong to obligate xenogamy(except the Department of Maye).]]> 2016/1/15 21:07:57 1, WANG Bing², JIAO En-Ning¹,]]> QIN Ken¹, WANG Bing², JIAO En-Ning¹, 23true Ardisia from China. The chromosome numbers 2n=46,karyotype 2n=46=42m+2sm+2st of A.crenata and the karyotype 2n=92=58m+24sm+10st of A.japonica were reported here for the first time. The karyotype formula of A.mamillata were 2n=44=40m+2sm+2st. The karyotype analysis showed that there were aneuploid variation of chromosome number and polyploidization evolution in Ardisia.]]> 2016/1/15 21:07:57 1, WANG Jun²]]> LI Yun-Xiang¹, WANG Jun² 22true Orchidantha chinensis and O.chinensis var.longisepala were studied for the first time in this paper. The results indicated that metaphase chromosomes of O.chinensis were ranged in length from 5.00 to 7.78 μm,while those of O.chinensis var.longisepala were ranged in length from 5.00 to 7.92 μm. The karyotype of the former was formulated as 2n=6x=54=23m+3sm(1sec)+1st(sec),that of the latter was formulated as 2n=6x=54=22m+4sm(2sec)+1st(sec). Both belong to Stebbins' 2A type. The conclusion that O.chinensis var. longisepala was considered as a variety was supported in light of the karyomorphological data in this paper.]]> 2016/1/15 21:07:57 1,2, SONG Juan-Juan¹, LIAO Jing-Ping¹, CHENG Zhong-Yi¹, TANG Yuan-Jiang^1*]]> MIN Ling-Li^1,2, SONG Juan-Juan¹, LIAO Jing-Ping¹, CHENG Zhong-Yi¹, TANG Yuan-Jiang^1* 21true Solanum photeinocarpum and S.nigrum var.suaveolens were studied by using DAPI banding technique. Somatic chromosome numbers of S.photeinocarpum are 2n 24(diploid),the karyotype formulas being K(2n)=24=6sm+18m; however,the chromosome numbers of S.nigrum var.suaveolens are 4n 48(tetraplont),the karyotype formulas being K(4n)=48=48m,and karyotype idiograms were established of the two species. The DAPI banded pattern and distribution of heterochromatic were also discussed that to improve the systematic classification and evolutionary trends research,breeding and exploiting the resources of S.nigrum in China.]]> 2016/1/15 21:07:57 *, XU Xiu-Fang]]> LAN Wei-Zhen, ZHANG Hai-Yang^*, XU Xiu-Fang 20true Plantago major and P.depressa in salinized soil by light microscope and paraffin section. The results show that there are some difference of anatomical structures between the two species:phellem layers of P.depressa is thinner than that of P.major,and phelloderm of the former is less than that of the latter; epidermis cuticle on the leaf of P.depressa is thiner,and substomatic chamber and parenchyma cells around the vascular bundle sheath are also smaller than that of P.major; the vascular bundle sheath of P.depressa consist of two layers of cell. Both of them have characteristic adapt to salt circumstances:rich aerenchyma in vegetative organs,developed phellem and parenchyma in secondary structure of roots,thick cuticula in leaf cortex.]]> 2016/1/15 21:07:57 WANG Yu-Liang, ZHENG Yu-Hua, ABDUSALERK·Nurbay 19true Dioscorea polystachya,eliminate the confusion of its cultivars and provide theoretical base for breeding,the micro-morphology of leaf abaxial epidermis of 33 germplasms of D.polystachya under optical microscope were studied. The results showed that the shapes of the leaf abaxial epidermis cells were all irregular and their varieties of sizes were remarkable. The anticlinal walls are straight,sinuolate,sinuous or sinuate. All of the stomatal apparatuses are anomocytic types and their shapes are elliptic and long-elliptic. Among the 33 different germplasms,the variation of stomatal size and index was small but the stomatal density was large. The‘Cell Form Coefficient',which was the area percent of a cell's periclinal wall to a circle which the perimeter is the same with the cell's anticlinal wall's,was tentatively suggested. From 19.31% to 70.60%,the value of Cell Form Coefficient can reflect the irregular level of epidermis cells quantificationally and four types were recognized according to the leaf abaxial epidermis micro-morphological characters of the 33 germplasms.]]> 2016/1/15 21:07:57 1, XU Zeng-Lai^1*, CHENG Shu-Bing², LI Bi-Yuan¹, WANG Qiong¹, SHI Yun-Yun¹]]> JIA Cheng-Sen¹, XU Zeng-Lai^1*, CHENG Shu-Bing², LI Bi-Yuan¹, WANG Qiong¹, SHI Yun-Yun¹ 18true Ranunculus(Ranunculaceae)is described from Yunnan, China.]]> 2016/1/15 21:07:42 1, LIAO Liang²]]> WANG Wen-Tsai¹, LIAO Liang² 17true Ornithochilus,Acanthephippium and Chiloschista,and nine species,Ornithochilus difformis(Lindl.)Schltr.,Acanthephippium sylhetense Lindl.,Chiloschista yunnanensis Schltr.,Bulbophyllum violaceolabellum Seidenf.,Hemipilia kwangsiensis T.Tang et F.T.Wang ex K.Y.Lang,Oberonia kwangsiensis Seidenf.,Cleisostoma menghaiense Z.H.Tsi,Pholidota longipes S.C.Chen et Z.H.Tsi,Calanthe sylvatica(Thou.)Lindl.are newly recorded from Guizhou Province. Voucher specimens are preserved in Herbarium of Maolan Reserve(HML).]]> 2016/1/15 21:07:42 WEI Lu-Ming, YU Deng-Li 16true Pinus yunnanensis and its closely related species,sampled from the south-east,mid-part and north-west of Yunnan Plateau,were extensively investigated,observed and analyzed. The shoot apex morphological characteristics of P.yunnanensis and its closely related species presented polymorphism in Yunnan plateau. The length and arrangement tightness of bud scales increased with the altitude heightening. The“anisomerous”needle primordia differentiated the different needle leaves per needle bundle. The morphological and structural polymorphism of P.yunnanensis. and its closely related species was the joint action of ecological environment complexity and ecological factor combination diversity.]]> 2016/1/15 21:07:42 1, WANG Jin^1,2, JIANG Han-Qiao²]]> WANG Chang-Ming¹, WANG Jin^1,2, JIANG Han-Qiao² 15true Pelargonium zonal ‘Ms.Pollock' were investigated for study on it's mechanism of variegation changes. The results showed that the changes of the types were not caused by new cell mutation,but by translocation of the different cell types of variegation. The changes of division direction of white cells and green cells resulted that some of the green epidermal cells translocated into the second layer and it pushed furthermore the origin white cells of the second layer into the third layer,so that the variegation altered.]]> 2016/1/15 21:07:42 LI Ming-Yin 14true 2016/1/15 21:07:42 1, LI Yan-Qun², MAI Shi-Qiu¹, MEI Zheng-Min¹, ZHANG She-Nan¹, HUANG Rong-Shao²]]> OU Shan-Han¹, LI Yan-Qun², MAI Shi-Qiu¹, MEI Zheng-Min¹, ZHANG She-Nan¹, HUANG Rong-Shao² 13true Taenitis blechnoides are observed under light microscope for the first time. The rationality of that Taenitidaece divided from Lindsaeaceae is discussed,as well as the systematic significance in the shape of prothallal border cell,the sensibility of chloroplasts to light,the shape of rhizoids and the formation and differentiation of the antheridium.]]> 2016/1/15 21:07:42 *]]> TAN Long-Yan, LIU Bao-Dong^* 12true Taiwania cryptomerioides,which distributed in Xingdoushan National Natural Reserve of Hubei Province. 13 primers were selected from the tested 60 random primers and 155 DNA fragments were scored. The percentage of polymorphic loci of the wild and cultivated populations were 65.81% and 59.35%,respectively. Nei's gene diversity and Shannon's information measure(H)of the wild population were 0.2001 and 0.3120,respectively; while the corresponding values of the cultivated one were 0.1911 and 0.2946,respectively. The genetic diversity of the wild population was higher than that of the cultivated one. The results showed that the cultivated population had a certain extent genetic recession. In the UPGMA dendrogram based on Nei's genetic distance,the individuals from the wild and cultivated populations clustered respectively. Preliminary study showed that T.cryptomerioides maintained a relatively high level of genetic diversity,though there were nearly 40 wild trees distributing fragmently in Hubei Province. This meant that the main cause threatening its natural population was not the low genetic diversity. The endangered status of this taxon was mainly attributed to the habitat deterioration and disturbance,and the poor natural regeneration ability caused by its own biological and ecological characteristics.]]> 2016/1/15 21:07:42 *]]> YANG Qin-Jun, CHEN Guang-Fu, LIU Xiu-Qun, CHEN Long-Qing^* 11true Sarcandra glabra. Based on the genomic DNA, some essential factors that affect the result of RAPD were compared and the optimal RAPD system for S.glabra was established. The results showed that the optimal concentration of five important components such as Mg²⁺,Taq DNA polymerase,primer,template DNA,and dNTP in 25 μL RAPD reaction system were 2.5 mmol/L,1.0U,0.4 μmol/L,60 ng,0.16 mmol/L respectively. Modified thermal profile consisted of an initial denaturation step at 94 ℃ for 2 mins,followed by 40 cycles of 94 ℃ for 30s,37 ℃ for 30 s,72 ℃ for 80 s,and a final exposure to 72 ℃ for 10mins.Then stored in 4 ℃ for 10 mins.]]> 2016/1/15 21:07:42 1, HE Ping^2,3]]> ZHANG Zhi-Yong¹, HE Ping^2,3 10true 2015/12/16 11:37:28 WANGWen-Tsai 9true 2015/12/16 11:37:28 1,2,HUZong-Gang³,MALü-Yi^1*,FUDe-Zhi²]]> SUNYing-Bao^1,2,HUZong-Gang³,MALü-Yi^1*,FUDe-Zhi² 8true 2015/12/16 11:37:28 1,QINHai-Ning^2*,XUEDa-Yuan^1,3,ZHOUKe-Xin¹]]> WUJian-Yong¹,QINHai-Ning^2*,XUEDa-Yuan^1,3,ZHOUKe-Xin¹ 7true 2015/12/16 11:37:28 CHENJian-Hui,WANGHou-Lin 6true 2015/12/16 11:37:28 DENGTao,ZHANGDai-Gui,CHENGong-Xi*,ZHUGui-Yu,HEHai-Sheng 5true 2015/12/16 11:37:28 LIHe,LIUSong-Mei,SUNYan,RUANCheng-Jiang 4true 2015/12/16 11:37:28 1,YANMu-Ting¹,YEChuang-Xing¹*,LIUSu-Qing²*]]> YEZi-Wei¹,YANMu-Ting¹,YEChuang-Xing¹*,LIUSu-Qing²* 3true 2015/12/16 11:37:28 1,WANGYu-Liang²,ZHENGYu-Hua²,KANGJian²]]> CHAIRui-Juan¹,WANGYu-Liang²,ZHENGYu-Hua²,KANGJian² 2true 2015/12/16 11:37:28 1,2,ZHAOQi¹,CHENYan-Ping¹,MENGZhen-Gui¹,LIRong-Chun^1*]]> ZHANGLing^1,2,ZHAOQi¹,CHENYan-Ping¹,MENGZhen-Gui¹,LIRong-Chun^1* 1true