2016/1/15 3:05:18 1,2, ZHAO Zhi-Guo¹, LU Feng-Lai¹, LIU Jin-Lei¹, CHEN Yue-Yuan¹, WEN Yong-Xin¹, LI Dian-Peng^1*, SUN Bu-Xiang³]]> LIANG Mei^1,2, ZHAO Zhi-Guo¹, LU Feng-Lai¹, LIU Jin-Lei¹, CHEN Yue-Yuan¹, WEN Yong-Xin¹, LI Dian-Peng^1*, SUN Bu-Xiang³ 38true Pyrrosia lingua were determined and analyzed. The results were as follows:(1)the functional composition content in the sporophyll was in a decreasing order of total flavonoides,polysaccharides and total saponins,and the content of total flavonoides differed significantly from the content of polysaccharides and total saponins(P<0.01),while the content of polysaccharides had no significant difference with the content of total saponins(P>0.05); the functional composition content in the foliage leaf was in a decreasing order of polysaccharides,total flavonoides and total saponins,and the differences of polysaccharides,total flavonoides and total saponins content were significant(P<0.01).(2)The contents of polysaccharides and total flavonoides were significantly different between the sporophyll and the foliage leaf(P<0.01),while the contents of total saponins in the sporophyll had no significant difference comparing with that in the foliage leaf(P>0.05),and the contents of polysaccharides in the foliage leaf were higher than that in the sporophyll,whereas the contents of total flavonoides in the sporophyll were higher than that in the foliage leaf.(3)There was significant negative correlation between the content of polysaccharides and total flavonoides in leaves of P.lingua(P<0.01),while the content of total saponins was not significantly related to the content of polysaccharides and total flavonoides(P>0.05).]]> 2016/1/15 3:05:18 1, YIN Xiao-Hong¹, HUANG Min¹, LI Yan-Qun¹, QIN Feng², HUANG Rong-Shao^1*]]> CHEN Chao-Jun¹, YIN Xiao-Hong¹, HUANG Min¹, LI Yan-Qun¹, QIN Feng², HUANG Rong-Shao^1* 37true Staphylococcus aureus and Bacillus subfilis. A moderate antibacterial activity was also observed against Escherichia coli. No antimicrobial activity against Lactobacillus Bulgaricus,Streptococcus thermophilus,Aapergillus sp.and Penicilllium sp.was observed. The autoclaved capsaicin had more antimicrobial activity though its effect had been weakened. The inhibitory effect was not changed with the culture's time. The minimum inhibitory concentration of capsaicin against B.subfilis was determined to be 50 mg/mL.]]> 2016/1/15 3:05:18 1*, TAO Neng-Guo¹, LI Jun-Li², LIU You-Xiu¹, LI Xiao-Ning¹]]> ZHANG Ji-Hong^1*, TAO Neng-Guo¹, LI Jun-Li², LIU You-Xiu¹, LI Xiao-Ning¹ 36true Houuuynia cordate assisted by ultrasound was researched,inhibition effects of flavonoid extraction on DPPH,hydroxyl radical,superoxide radical were also studied. The results showed that the flavonioid content in the leaves was the highest,and under the optimal conditions(the ratio of solid to liquid is 1:30,alcohol 85% at 65 ℃ for 40 min),the yield of flavonoid was 2.36%. H.cordate flavonoid possessed strong scavenging activity on DPPH,hydroxyl radical,superoxide radical with corresponding maximum scavenging rate 72.8%,70.8% and 69.8%,suggesting that it would be an effective natural free radical scavenger.]]> 2016/1/15 3:05:18 *, HUANG Yan]]> ZHANG Jian, ZENG Hong-Yan^*, HUANG Yan 35true Camellia nitidissima were analyzed by spectrophotometer, plasma atomic emission spectrometry, and automatic amino-acid analyzer. Main nutritional components in C. Nitidissima were carbohydrates, and the contents of water soluble sugar and crude fiber were rich. The content of fat, crude fiber and water soluble sugar of C. nitidissima increased gradually along with the formation of the bud to the full bloom stage, descended just at wither stage. The contents of flavonoids, saponins, catechins, VE in flower were higher than those in the bud and the just wither stage. Total amino acid of buds, full bloom and just wither stages were 7.44, 5.14, 5.00 g·100 g^-1. C. nitidissima contained rich amino acids, bud contained more amino acids. The flowers of C. nitidissima have a high value for exploitation and utilization. It provides the scientific basis for the development and utilization of C. nitidissima flowers in this study.]]> 2017/10/1 18:46:42 1, SHI Yan-Cai¹, LIAO Yu-Qiong³, FAN Jin-Shun¹, WEI Ji-Qing^1*, WEN Xiang-Ying², WEI Xiao¹]]> TANG Jian-Min¹, SHI Yan-Cai¹, LIAO Yu-Qiong³, FAN Jin-Shun¹, WEI Ji-Qing^1*, WEN Xiang-Ying², WEI Xiao¹ 34true Dendrobium crystallinum were isolated by silica gel, Sephadex LH-20 column chromatography and reversed-phase semi-HPLC. Their structures were elucidated by analyzing their spectral data and comparing with the previously reported literatures. Ten compounds were identified as gigantol(1), 3,4'-dihydroxy-5-methoxydihydrostilbene(2), 3,4',5- trihydroxy-3-methoxydihydrostilbene(3), dihydroresveratrol(4), angophorol(5), 3',5,7- trihydroxy-4'-methoxyflavanone(6), 4',5,7-trihydroxy-6-methoxyavanone(7), syringaresinol(8), β-sitosterol(9), β-daucosterol(10). All compounds were firstly isolated from this plant except for compounds 2 and 10.]]> 2017/10/1 18:46:42 1, CHENG Zhong-Quan¹, DING Zhong-Tao³, ZHOU Jun², HU Jiang-Miao^2*]]> YANG Dan¹, CHENG Zhong-Quan¹, DING Zhong-Tao³, ZHOU Jun², HU Jiang-Miao^2* 33true Ziziphus jujube cv. Lingwuchangzao at mature stage from different areas in Ningxia(Lingwu, Yinchuan, Zhongning, Qingtongxia)were used as the experimental materials. The water extraction and alcohol precipitation method were adopted to obtain polysaccharide, which was subsequently purified by DEAE-52 cellulose column and HW-55S gel column. Finally gas chromatography-mass spectrography(GC-MS)was used to analyze the monosaccharide composition from purifed polysaccharides. The results showed that the crude polysaccharides from Lingwu had the highest yield, 1.795%. After purifed, all polysaccharides were fractioned respectively to one neutral fraction,Ju-0, and three acid fractions,Ju-1, Ju-2, Ju-3,and Ju-2 had the highest contents. This indicated that the main components of polysaccharides in Z. jujube cv. Lingwuchangzao were acid fractions. The results from GC-MS showed that Z. jujube cv. Lingwuchangzao were composed of arabinose, rhamnose, ribose, fucose, xylose, mannose, galactose, glucose, glucuronic acid and galacturonic acid, but monosaccharide contents were different among different fractions. Arabinose, ribose, galactose, glucuronic acid and galacturonic acid were the main components, and the content of xylose was the lowest. Both Ju-0 and Ju-1 were mainly composed by arabinose, ribose, galactose, while Ju-2 and Ju-3 exist obvious differences between four areas. According to the comparision of the total contents of monosaccharides, the content of arabinose in polysaccharides from Lingwu reached the highest, accounting for 28.6% of the total contents of monosaccharides, but other areas only accounted for 13%-18.9%. Meanwhile, glucuronic acid reached 21%-26.5% in polysaccharides from other three areas, but it was only 2.6% in polysaccharides from Lingwu. This indicates that the key difference in monosaccharide composition of polysaccharides from different areas lies in the content of arabinose and glucuronic acid. The advantages of polysaccharides in Z. jujube cv. Lingwuchangzao from Lingwu mainly are high content of arabinose and low content of glucuronic acid, which is likely to be one of key factors of the difference in fruit quality. This study will provide basis and scientific support for further of development and utlization of the fruits of Z. jujube cv. Lingwuchangzao.]]> 2017/10/1 18:46:42 *]]> CHAI Ya-Hong, ZHANG Ying-Cai^* 32true Oxalis corniculata, and to provide comprehensive evaluation of their quality in different regions, the fingerprint of O. corniculata was established by UPLC. The similarity evaluation and cluster analysis were used to deal with the experimental data, in order to find out the similarities and differences among the 30 batches of O. corniculata from 15 different areas. The specific chromatogram of O. corniculata was obtained, and 24 common peaks were identified. Similarities of the 30 batches of samples were 0.839-0.987. The results of cluster analysis were the 27 batches of samples content of chemical components were closer, in addition to samples of Jianhe County, Yinjiang County of Guizhou Province and Tianzhu County of Guizhou Province in August. The establishment of UPLC fingerprint of O. corniculata can provide a more comprehensive and faster reference for the quality control of Oxalis. This method is simple, rapid and reliable. It has higher sensitivity and resolution than HPLC and greatly shortens the detection time.]]> 2017/10/1 18:46:42 1,2, FENG Hua³, WU Hong-Mei², TANG Rong-Rong², WANG Xiang-Pei^2*]]> WU Lin-Jing^1,2, FENG Hua³, WU Hong-Mei², TANG Rong-Rong², WANG Xiang-Pei^2* 31true Cannabis is one of the most common drugs of abuse worldwide. The major cannabinoids in cannabis plants are relatively unstable and their contents may vary in different storage conditions. The aim of the present study was to investigate the effects of light and temperature on the stability of cannabinoids in Cannabis plants. The levels of Δ9-tetrahydrocannabinol(Δ9-THC), cannabinol(CBN)and cannabidiol(CBD)in different chemical phenotypes cannabis powders and methanol extracts were respectively measured for up to 20 d storage in natural light at laboratory temperature(22 ± 2)℃ and in darkness at laboratory temperature(22 ± 2)℃, 4 ℃ and -20 ℃, using the method of ultra-high performance liquid chromatography(UPLC-PDA). The results showed that the changes in contents of the three cannabinoids in different chemical phenotypes were the same. The degradation of Δ9-THC and CBD were pronounced and the content of CBN was almost unchanged for the solid samples exposed to light at(22 ± 2)℃. The contents of Δ9-THC, CBN and CBD in methanol extracts exposed to light at(22 ± 2)℃ were significantly decreased. Cannabis plants and Cannabis extracts can be stored in darkness at laboratory temperature(22 ± 2)℃, 4 ℃ and -20 ℃. The degradation of the main psychoactive component Δ9-THC in Cannabis satisfied the rule of first-order reaction kinetics, and the above-mentioned regular pattern was used to study the change of the contents of Δ9-THC in different storage conditions. It is concluded that light is an important factor affecting the degradation of Δ9-THC. Cannabis powders or extracts are reasonably stable if stored in the dark at room temperature. It is better to guide the collection, transportation, preservation and identification of Cannabis materials in judicial practice in a short period of time.]]> 2017/10/1 18:46:42 1, ZHAO Yan-Biao², ZHENG Xiao-Yu², ZENG Ling-Hua¹, GAO Li-Sheng², LIU Yao^1,2, ZHENG Hui^2*]]> SUN Wei-Lai¹, ZHAO Yan-Biao², ZHENG Xiao-Yu², ZENG Ling-Hua¹, GAO Li-Sheng², LIU Yao^1,2, ZHENG Hui^2* 30true Flemingia polysaccharide on immuno compromised by cyclophosphamide and normal mice. We measured thymus, spleen and calculated the visceral index; measured mononuclear-macrophage phagocytic function by the Carbon Grain Kuo Clear Law; measured the level of serum hemolysin after erythrocyte immune, to observe the effect of the high and low dose of Flemingia polysaccharide(doses of 500, 1 000 kg·d^-1)on the normal SPF BALB/c mice. We injected cyclophosphamide(40 mg·kg^-1)five times subcutaneously every other day to lead to the immunosuppression mice. We measured thymus, spleen and calculated the visceral index; measured mononuclear-macrophage phagocytic function by the Carbon Grain Kuo Clear Law; measured the level of serum hemolysin after erythrocyte immune, observe the effect of the high and low dose of Flemingia polysaccharide on the immunocompromised SPF BALB/c mice. High and low dose of Flemingia polysaccharide could increase index of immune organs, carbon clearance capacity in varying degress(P<0.05 or P<0.01), and could also enhance the clearance index on normal and immunocompromised mice, but it have no significance, and could increase antibody of serum hemolysin significantly(P<0.05 or P<0.01)as well. These indicate that Flemingia polysaccharide can obviously enhance the immune function in normal and immuno compromised mice.]]> 2017/10/1 18:46:42 1, QIAO Xue², YANG Zi-Ming³, LU Yu-Ting¹, QIN Hai-Guang^2*]]> ZHUO Shen¹, QIAO Xue², YANG Zi-Ming³, LU Yu-Ting¹, QIN Hai-Guang^2* 29true Artemisia annua contains abundant essential oils. It is commonly used as traditional Chinese medicine for treating dysentery. The varietal complexity and vague provenance boundaries of the species of A. annua seriously affected the quality of medicinal materials. To investigate the rapid identification and evaluation of A. annua and sibling species leaves on the yields and compositions of essential oils, the volatile compounds were detected and identified by GC-MS and FTIR analysis. A. annua determined to have the highest yield(1.86%)of essential oil, followed by A. eriopoda, A. capillarie, A. apiacea, A. japonica and A. argyi. FTIR results showed that one-dimensional spectrum was similarity of A. annua and sibling species and the species were abundant of amides, aromatics and terpenoids. Second derivative spectrum of A. annua component vibration absorption enhanced obviously to clearly demarcation of A. annua and confusion. GC-MS analysis showed that the volatile oil of A. annua and sibling species revealed the identification of 28 components and 17 common peaks. A. annua and sibling species leaf oils contained high amounts of camphor, á-Cadinene, crocetane, phytan, 2, 4-di-t-Butylphenol. The results indicated the apparent difference in the volatile compound compositions of essential oils between species. However, the content of phytane of A. annua was significantly higher than that of other species. Borneol was only detected in A. annua. In addition, A. apiacea, A. eriopoda and A. capillaries contained the highest á-Cedrene content, while A. annua, A. argyi and A. japonica contained the lowest content of á-cedrene. The oil components were analyzed using a hierarchical cluster for the six samples and samples were divided into two main clusters, A. annua, A. japonica and A. argyi was distinguished as a cluster, while A. apiacea, A. eriopoda and A. capillaries were classified as a cluster. These results provide an effective analysis methods for identifying the species, especially for the quality assessment for use in raw herbal medicines.]]> 2017/3/8 14:17:08 1, LI Yan-Qun², ZOU Rong¹, SHI Yan-Cai¹, WEI Xiao^1*]]> KONG De-Xin¹, LI Yan-Qun², ZOU Rong¹, SHI Yan-Cai¹, WEI Xiao^1* 28true Xanthoria elegans, Myelochroa indica, Ramalina peruviana, Cladonia macilenta, Nephromopsis pallescens and Cladonia coccifera)were cultured in liquid medium for two months. The secondary metabolites of lichen forming fungi were obtained by extracting with ethyl acetate, and then the extracts were weighed and dissolved in DMSO. Seven kinds of pathogenic bacteria(Bacillus subtilis, B. cereus, Vibrio parahaemolyticus, Straphylococcus haemolyticus, Pseudomonas aeruginosa, Staphylococcus aureus and Micrococcus luteus)were used to screen activity of the extracts. Inoculating inhibition zone was used to evaluate the antibacterial activity of the extracts, and the minimum inhibitory concentration with antibacterial activity was also detected. The results showed that all of primary extract of lichen forming fungi had extensive antibacterial activity, and the antibacterial activity of the same fungi cultured showed significantly difference in different media. The secondary metabolites produced by Ramalina peruviana cultured in MY liquid medium had inhibitory effect to Straphylococcus aureus, Straphylococcus haemolyticus, Pseudomonas aeruginosa and Micrococcus luteus. It showed that the primary extract from Ramalina peruviana had extensive antibacterial effect. But the secondary metabolites of R. peruviana cultured in YMG liquid medium had not inhibitory effect to all of these pathogenic bacteria. Extracts from all of lichen forming fungi cultured in YMG liquid medium, Only the extract of Cladonia coccifera inhibited the Staphylococcus aureus which is gram-negative bacteria. The primary extract produced by Xanthoria elegans cultured in YMG medium had antibacterial effect to Bacillus cereus, the diameter of inhibition zone was up to 17.77 mm. Our results confirmed that the secondary metabolites of lichen forming fungi have antimicrobial activity and the lichen forming fungi can produce bioactive secondary metabolites in laboratorial culture conditions. Experiment results also showed that different media can stimulate different lichen forming fungi to produce different components. The innovation of this study was that different media were used to culture lichen forming fungi and lichen forming fungi had the characteristics of one strain many compounds. This study provides the groundwork to identify the active ingredients from lichens and provides an important information for culturing mass lichens to obtain bioactive compounds.]]> 2017/3/8 14:17:08 1, 2, YUAN Xiao-Long^2,3, HUA Mei^2,3, LI Su-Yu^1,2, WANG Juan^2,3, WANG Yi^2,3*]]> ZHAO Neng^{1, 2}, YUAN Xiao-Long^2,3, HUA Mei^2,3, LI Su-Yu^1,2, WANG Juan^2,3, WANG Yi^2,3* 27true -1 of methacrylic acid, 25 g·L^-1 of lignin, 3.25 × 10^-2 mol·L^-1 of crosslinker, 1.25 × 10^-2 mol·L^-1 of catalyst and 65 ℃ of reaction temperature, the synthesized hydrogel possessed a maximum swelling ratio of 28.16 g·g^-1 in simulated intestinal fluid. Compared with polymethacrylic acid hydrogels without of bagasse lignin, the swelling ratio of bagasse lignin/polymethacrylic acid hydrogels all were decreased moderately at different pH conditions. However, the addition of bagasse lignin made the sensitive pH of the hydrogels increased from pH 4-5 to pH 6-8.(2)Results of swelling-deswelling experiments showed that the addition of lignin made the swelling-deswelling reversibility of hydrogel vary by the components. However, compared to polymethacrylic acid hydrogels, bagasse lignin/polymethacrylic acid hydrogel showed more sensitive response to pH and reached equilibrium in a shorter period of time.(3)Results of adsorption tests showed that the addition of lignin to the hydrogel may increase the adsorption amount of bovine serum albumin. Among of the tested bagasse lignin/polymethacrylic acid hydrogel samples, the maximum loading capacity for bovine serum albumin was found to be 577 mg·g^-1.(4)Results of controlled release tests showed that the release equilibrium for BSA could be reached after 12 h with a release rate of 10% in simulated gastric fluid and 92% in simulated intestinal fluid, respectively. As a natural and renewable resource, the utilization of bagasse lignin have been interested by researchers. Results of this study showed bagasse lignin/polymethacrylic acid hydrogels may be used as an potential polymer carrier for oral delivery of protein drugs and provided a possible way for the utilization of bagasse lignin.]]> 2017/3/8 14:17:08 1, CHEN Hai-Shan², LI Zi-Yuan¹, LI Hai-Yun^1*]]> YUAN Zhi-Lin¹, CHEN Hai-Shan², LI Zi-Yuan¹, LI Hai-Yun^1* 26true Rosa laevigata were the main ingredients of the Sanjin tablet, at present, the studies on the chemical constituents and pharmacological of the R. laevigata were relatively insufficient. In order to elucidate the material basis and the activity of R. laevigata, the constituents of R. laevigata were isolated by silica gel, Sephadex LH-20, MCI gel CHP 20P column chromatography and semi-preparative HPLC. Their structures were elucidated by anlalyzing their spectral data and comparing with the previously reported literatures. Nine compounds:(+)-catechin(1),(-)-epicatechin(2), rosamultin(3), sericoside(4), 2α, 3α, 19α, 23-tetrahydroxy-urs-12-en-28-oic acid-3-O-β-D-glucopyranosyl ester(5), kaji-ichigoside F1(6), β-D-Glucopyranosy 3β,19α-dihydroxy-2-oxo-urs-12-en-28-oate(7), daucosterol(8), β-sitosterol(9)were obtained. Compounds(2),(4),(5)and(7)were reported from the plant for the first time. The results provide scientific information for exploitation and medicine utilization of R. laevigata.]]> 2017/3/8 14:17:08 1,2, HUANG Yong-Lin¹, LIU Jin-Lei¹, WANG Lei¹, YAN Xiao-Jie¹, LI Dian-Peng^1*]]> DING Yang^1,2, HUANG Yong-Lin¹, LIU Jin-Lei¹, WANG Lei¹, YAN Xiao-Jie¹, LI Dian-Peng^1* 25true Ardisia quinquegona to provide experimental basis for the Zhuang medicine quality evaluation and standard. The different habitats of A. quinquegona were identified by TLC. The contents of Bergenin in different habitats and parts of A. quinquegona were determinated by HPLC. Using SinoChrom ODS-BP C18 chromatographic column(250 mm × 4.6, 5 μm)with the detection wavelength of 275 nm, methanol-water(23:77)as mobile phase with the flow rate of 1.0 mL·min^-1. The results showed that ten batches different eares of A. quinquegona which contained Bergenin by TLC. And the average content of bergenin was 1.67%, but there were significant differences among them, the highest content up to 2.58%, the lowest content 0.95%. The contents of A. quinquegona of different parts were different, the underground part(root and rhizome)contents higher with an average of 4.05%, the ground parts(stems and leaves)were lower with an average of 1.21%. TLC identification method and determination of Bergenin in A. quinquegona were simple, reproductive and accurat. The chemical method could accurately identify the authenticity of A. quinquegona and determination in Bergenin. This study provides experimental basis for the establishment of A. quinquegona medicinal Zhuang medicine quality standards for the control of its quality is of great significance.]]> 2017/3/8 14:17:09 1, LI Zheng-Wen¹, HUANG Rui-Song^2*]]> ZHANG Zhen¹, LI Zheng-Wen¹, HUANG Rui-Song^2* 24true Moghania with the orthogonal design, we compared the deproteinization process conditions of polysaccharides by Sevage with TCA method, determined the total polysaccharide contents with the method of phenol-sulfuricacid and the protein content by the method of Coomassie brilliant blue. And we also purified the Moghania polysaccharide by DEAE-52 and analyzed the monosaccharide compositionby of Moghania polysaccharide by the method of high performance liquid chromatography(HPLC). The results showed that the best time, ratio of solid to liquid and extraction temperate of the extraction process were in 2.5 h, 1:30, 80 ℃, as the extraction condition, the yield of polysaccharide was 8.558%. By comparison with the Sevage method and TCA method, the optimal conditions of deproteinization were proved to be extracted three times with Sevage method. Moghania polysaccharide was isolated into seven fractionates by DEAE-52 cellulose column chromatography; three kinds of saccharide were investigated by the HPLC, which were glucose, mannose and arabinose. In the three kinds of saccharide, peak area of the glucose was biggest than others, so the major saccharides was glucose.]]> 2017/3/8 14:17:09 1, ZHUO Shen², YANG Zi-Ming³, LU Yu-Ting², QIN Hai-Guang^4*]]> QIAO Xue¹, ZHUO Shen², YANG Zi-Ming³, LU Yu-Ting², QIN Hai-Guang^4* 23true Pinus massoniana is one of the most important tree species for afforestation in South China, and it is of great economic value and ecological benefit. This study aimed to reveal the cytological basis in relation to induced resistance during the biosynthesis of terpenoids in P. massoniana, and provide the guide for the further research on molecular mechanism of induced insect resistance in P. massoniana. In the illumination incubator with strictly controlled conditions of light and temperature, variations of microscopic structure, terpenoid synthetase activity and its cytochemical localization of needles were investigated in P. massoniana seedlings treated by exogenous jasmonates. The results showed that there were increases in the relative contents of terpenoids, especially, in those of monoterpenoids and diterpenoids, and the antifeedant of Dendrolimus punctatus against needles in Pinus massoniana seedlings was increased following the treatment of 0.2 mmol·L^-1 MeJA, which indicated that the induced resistance of P. massoniana were strengthened. For the variations of anatomical structure, secretions of resin canal were enhanced, the number of chloroplast was reduced, the volume of it was increased, and the thylakoid of it was enhanced in the needles of P. massoniana seedlings. The activity of terpenoid synthetase was increased after four weeks of MeJA treatment. The localization of terpenoid synthetase activity was remarkably increased from membrane system, especially, from chloroplast membrane in the needles of P. massoniana seedlings using the method for electron microscopic cytochemistry of enzyme. This implies that the induced resistance is closely related to the changed chloroplast structure and terpenoid synthetase activity in green plastids in P. massaniana exposed to MeJA treatment.]]> 2018/7/28 16:52:33 *]]> YAO Ruiling, LI Huijuan, ZHANG Xiaoning, WANG Yin^* 22true Bauhinia championii, a traditional Chinese herbal medicine, has effects on anti-inflammatory, analgesic, eliminate free radicals, anti-bacterial, anti-tumor and so on. In order to further broaden the potential fungicidal and herbicidal activities of different constituents of B. championii, we attempted to dry and smash B. championii leaves and used methanol to extract for three times at the normal temperature. Crude extract was separated and purified on silica gel column chromatography. A bottle of fraction was collected every 200 mL, according to TLC detection and iodine cylinder integration, the crude extract was separated into nine constituents. The precipitated material in constituents No. 7 and No. 8 were recrystallized repeatedly, and melting points were confirmed and measured by pointing GF254 silica gel plate. One pure compound was obtained and named No. 33. Based on data analysis and compared with molbase library, this compound was identified as 1-D-4-O-methyl-myo-insitol, an important industrial raw material. The test results of fungicidal and herbicidal activities showed the crude extract of B. championii leaves to inhibition rate of Pyricularia oryae was(40.84±1.00)% at 1 000 μg·mL^-1, and inhibition rate of Echinochloa crusgalli roots was(49.18±2.33)%. When the constituents were at 500 μg·mL^-1, No. 4 constituent of Pyricularia oryae inhibition rate reached(44.19±0.76)%, inhibition rate of No. 2 and No. 3 constituents on Echinochloa crusgalli roots were(88.92±1.31)% and(90.99±1.45)%, respectively; inhibition rate of No. 3 and No. 6 constituents on Portulaca oleracea roots were(72.06±1.31)% and(89.92±1.73)%, respectively. The results indicate that the extract of Bauhinia championii leaves have a certain amount of inhibitory effects on Pyricularia oryae, Echinochloa crusgalli roots and Portulaca oleracea roots. In order to acquire high activity monomer compounds, the constituents of No. 2, No. 3, No. 4 and No. 6 can be further separated.]]> 2018/7/28 16:52:33 1,2, WU Qinglai^1,2, KE Jian^1,2, HUANG Guan^1,2, DU Xiaoying^1,2, Li Junkai^1,2*]]> YAO Zongli^1,2, WU Qinglai^1,2, KE Jian^1,2, HUANG Guan^1,2, DU Xiaoying^1,2, Li Junkai^1,2* 21true TP53 and its downstream genes p16Ink4a, p21Waf1 / Cip1 and p27Kip1 were significantly up-regulated, accompanied by transactivation of other senescence signaling pathway related genes or senescence response genes such as ABL1, ALDH1A3, CHEK2, HRAS, PTEN, etc., while the expression of Cyclin A2, IGFBP3, SIRT1 and TERT, the genes which negatively regulated cell cycle progression and senescence signaling, were significantly down-regulated. Western blotting verified that protein levels of p53 and its downstream CKIs, p21WAF1 and p16INK4 increased while Cyclin A2 decreased, consistent with the findings in PCR results. The level of wild-type p53-induced protein phosphatase 1(Wip1)was also found significantly increased, suggesting that the induction of senescence in HepG2 cells by curcumol might be through activation of p53 signaling pathway. The present study further demonstrates that curcumol is capable of inducing cellular senescent phenotype in HepG2, accompanying with cell cycle G0 / G1 phase arrest.]]> 2018/7/28 16:52:33 1,2, YANG Feicheng³, YANG Jing^1,2, JIANG Xiaoshan^1,4*]]> HUANG Lanzhen^1,2, YANG Feicheng³, YANG Jing^1,2, JIANG Xiaoshan^1,4* 20true Malania oleifera is an endemic and rare species in China. In order to explore and utilize the antibacterial active compounds of endophytic fungi from M. oleifera bark, endophytic fungi(Acrostalagmus luteoalbus, Trichoderma harzianum,Periconia macrospinosa,Cladosporium cladosporioides,Pestalotiopsis maculans,Penicillium ochrochloron, Purpureocillium lilacinum, Penicillium minioluteum, Xenoacremonium recifei and Xylaria feejeensis)from Malania oleifera were cultured in liquid media. After 10 d culture, the culture media were collected, and then the crude extracts were obtained with ethyl acetate extraction. The inoculating inhibition zone was used to evaluate the antibacterial activity of the crude extracts. Meanwhile, the minimum inhibitory concentration(MIC)of antibacterial activity was also detected. The results showed that the crude extracts of Acrostalagmus luteoalbus, Periconia macrospinosa, Cladosporium cladosporioides,Pestalotiopsis maculans, Penicillium ochrochloron and Purpureocillium lilacinum had antibacterial activities, the crude extracts of Periconia macrospinosa, Pestalotiopsis maculans and Purpureocillium lilacinum had significant antibacterial activities against Bacillus lentus, Streptcococcus agalactiae and Micrococcus luteus. The minimum inhibitory concentration was between 1.562 5 and 6.25 mg·mL^-1. This results showed that secondary metabolites of endophytic fungi of Malania oleifera bark had antibacterial activities and the antibacterial effects of secondary metabolites of endophytic fungi were different.]]> 2018/7/28 16:52:33 1,2, HUA Mei¹, YUAN Xiaolong¹, QIU Jian², ZHENG Ke¹, WANG Yi^1*]]> XIAO Zhiye^1,2, HUA Mei¹, YUAN Xiaolong¹, QIU Jian², ZHENG Ke¹, WANG Yi^1* 19true Aralia chinensis stem-bark polysaccharides(ACP)were from Hui County of Longnan City in Gansu Province, China. Chemical modified A. chinensis stem-bark polysaccharide(ACP)was prepared, and the structure-activity relationship was studied. By chlorosulfonic acid pyridine method(CSA/Py), A. chinensis root-bark polysaccharide sulfation(SACP)was synthesized. The optimum reaction conditions determined by the response surface(RSM)experiments were as follows: the ratio of CSA/Py was 2.53, the reaction time was 5.23 h, and the reaction temperature was 61.25 ℃. Under such conditions, the sulfated polysaccharides with the degree of substitution(DS)of 0.57 were obtained, which was in accordance with the expectations of the model. The result of infrared spectroscopy(FT-IR)and X ray photoelectron spectroscopy(XPS)showed that sulfuric acid groups had been introduced into SACP, and S existed in the form of S⁶⁺. The average molecular weight(Mw)which was texted by size exclusion chromatography with laser light scattering method(SEC-LLS)showed that acidic reaction conditions caused the reduction of Mw. Gas chromatography mass spectrometry(GC-MS)analysis showed that monosaccharide composition of SACP were arabinose, glucose, galactose and mannose. The results of antioxidant activities of SACP and ACP in vitro showed scavenging ability on DPPH radical, hydroxyl radical, superoxide radical in a dose dependent manner, and good potential for reducing power. The free radicals scavenging ability of SACP was higher than that of ACP, which indicated that the introduction of sulfuric acid groups could enhance antioxidant ability of ACP. The above study revealed the structure of ACP and ACSP, and the effect of chemical modification on the antioxidant activity of ACP.]]> 2018/7/28 16:52:33 Aralia chinensis root-bark and its antioxidant activity]]> Preparation and structural characteristics of sulfated polysaccharides from Aralia chinensis root-bark and its antioxidant activity 18true Ruppia maritima collected from Beihai City and antifungal activities against Penicilliosis marneffei. The endophytic and rhizosphere bacteria isolated from Ruppia maritima were analyzed by method of dilution butteron on plate. 16S rRNA gene sequencing was employed to explore species diversities and the effects of the crude extract from bacteria against Penicilliosis marneffei with agar diffusion method and spectrophotometer method. Total of 26 strains of endophytic bacteria and 31 strains of rhizosphere bacteria were isolated from Ruppia maritina. Thirteen species of endophytic bacteria were obtained and classified into twelve genera and ten families. Nineteen species of bacteria were isolated from rhizosphere and belonging to fourteen generas and nine families. And five strains in the rhizosphere were potential new genera. Crude extracts of eight strains showed inhibition effect against Penicilliosis marneffei, three of which had strong antifungal activities, and their MIC₅₀ were(1.800 ± 0.045),(1.881 ± 0.061)and(1.604 ± 0.021)mg·mL^-1. Endophytic and rhizospheric bacteria of Ruppia maritima are genetically diverse and most of them showed strong inhibition effects against Penicilliosis marneffei.]]> 2018/7/28 16:52:33 1, GAO Chenghai^1,2, YU Lian³, LI Jiayi^1,3, YI Xiangxi^2*]]> LI Fei¹, GAO Chenghai^1,2, YU Lian³, LI Jiayi^1,3, YI Xiangxi^2* 17true Camellia sasanqua cultivars by solid phase micro-extraction and gas chromatography-mass spectrometry(GC-MS). The results showed that there were 29 components identified in ‘Dongxing', 24 in ‘Xiaomeigui', 42 in ‘Dongmeigui', 25 in ‘Zhaohe Zhirong' and 31 in ‘Xinyinv'. The volatile components and relative contents had greater differences among the five C. sasanqua cultivars, but the main characteristic volatile components of them were all acetophenone, cis-linaloloxide and linalool, and the main component types were alcohols, aldehydes and ketones. There were 21 components identified in ‘Fushi Zhifeng' with formal double. The main characteristic volatile components of ‘Fushi Zhifeng' were cis-linaloloxide, eugenol, cyclohexanone andtetradecane, and the main component types were alcohols, then alkanes. Analysis of volatile components showed obvious differences. These indicate that there are obvious differences of volatile components kinds and their relative contents of different C. sasanqua cultivars; Petals and stamens are the main flower parts of volatile components releasing.]]> 2018/7/28 16:52:33 *, FAN Zhengqi, YIN Hengfu, LI Jiyuan]]> WANG Jie, LI Xinlei^*, FAN Zhengqi, YIN Hengfu, LI Jiyuan 16true Kadsura coccinea and were comprehensively studied. The volatile compounds from three different parts which were roots, stems and leaves of K. coccinea were extracted by steam distillations(Method a)and headspace solid phase micro-extraction(SPME)(Method b). Volatile components of K. coccinea were analyzed by combining gas chromate-graphy-mass spectrometry(GC-MS)and the technology of computer retrieva. Antioxidant activities of volatile components were tested by DPPH free radical scavengng activity. The results were as follows:(1)There were some differences between species and contents of volatile components. And 98 and 117 volatile compounds were identified from three parts of K. coccinea through methods a and b. Among them, there were 59 and 68 kinds volatile components(accounting for 83.5%, 93.8% of root total content)in root, 54 and 56 species volatile components(accounting for 88.9%, 94.9% of stem total content)in the stem and 36 and 42 species in the leaf(accounting for 89.6%, 97.7% of leaf total content).(2)Species and contents of volatile components of three parts of K. coccinea through methods a and b were basically the same. The main volatile components were caryophyllene, pinene, elemene, cubebene, copaene, cadinene and d-Nerolidol(contents ≥70%).(3)Scavenging efficiency for DPPH free radical was more than 97% when the concentration of volatile components of root, stem and leaf of K. coccinea reached 20 mg·mL^-1, and volatile components of K. coccinea showed good antioxidant activity.]]> 2018/7/28 16:52:33 *]]> YANG Yan, GAO Jianfei^* 15true Geastrum fimbriatum is a kind of medicinal mushroom with hemostasis, clearing lung and alexipharmic functions. For the further study on the development and utilization of G. fimbriatum, the antioxidant, antibacterial and anticancer activities and the chemical constituents of the ethanol extract(EE)from G. fimbriatum were evaluated. The present study exhibited that the total phenolic content of EE was 10.53 μg·mg^-1 EE DPPH and ·OH radical-scavenging activities with IC₅₀ values of 91.35 and 148.76 μg·mL^-1, respectively. EE showed no antibacterial activity on Staphylococcus aureus, Escherichia coli and Bacillus subtilis. Meanwhile, EE displayed antifungal activity on Fusarium avenaceum with inhibition ratio of 36.11% at the concentration of 1 mg·mL^-1. EE displayed anticancer activities against tumor cells BG-803, NCI-H502 and MDA-MB-231 with the inhibition ratio of 18.87%, 17.71% and 41.23% respectively at the concentration of 200 μg·mL^-1(MTT assay). Six compounds were isolated from EE by the methods of column chromatography, thin layer chromatography, recrystallization and so on. The structures were elucidated by the analysis of spectral data and physical-chemical properties and identified as 5α, 8α-epidioxyergosta-6, 9(11), 22-triene-3β-ol(1), 5α, 8α-epidioxyergosta-6,22-dien-3β-ol(2), D-allitol(3), L-glutamic acid(4), maltose(5), saccharose(6). All the six compounds were isolated from G. fimbriatum for the first time.]]> 2018/7/28 16:52:33 1, 2, LIU Mingfei³, WANG Junli^3*, YU Zixiao⁴]]> LIU Kun^{1, 2}, LIU Mingfei³, WANG Junli^3*, YU Zixiao⁴ 14true Aloe barbadensis, i.e., the concentrated dry matter of the juice of the leaves of A. barbadensis, a systematic qualitative analysis of them was conducted using the technique of HPLC-DAD-ESI-IT-TOF-MSⁿ in conjunction with the comparison of reference compounds and literature search. The gradient elution was performed with water(A)-acetonitrile(B)as mobile phase at a flow rate of 1.0 mL·min^-1. The liquid chromatography-mass spectrometry data were acquired under alternate negative ion and positive ion detection mode using an ESI ion source. The structure elucidation of the chemical constituents was mainly based on negative ion mass spectrometry data. The results were as follows:(1)For the first time, the fragmentation pathways of anthraquinones(aloe-emodin, physcion, and emodin-8-O-β-D-glucoside), anthrones(aloin A, aloinoside A), chromones(aloeresin D, 7-O-methylaloeresin A, altechromone A, aloesin, aloeresin G, and aloeresin C), and α-pyranones(aloenin A, aloenin B)in Aloe made from A. barbadensis were clarified. The fragmentation pathway of anthraquinones was dominated by loss of CO₂ and CO, and that of anthrones was dominated by cleavage of hexosides and loss of CO. The fragmentation pathways of chromones was dominated by cleavage of hexosides and hydrolysis of the ester group, and that of α-pyranones was dominated by cleavage of hexosides and loss of CO₂ and H₂O.(2)A total of 168 chemical constituents of Aloe made from A. barbadensis were detected, and 78 of them were identified on the basis of reference compound comparison, literature retrieval, and chemical database(such as SciFinder)searching. The 78 compounds included 3 anthraquinones, 29 anthrones, 35 chromones, 7 α-pyranones and 4 other constituents. Twenty-three of 78 compounds were discovered in the leaves of A. barbadensis for the first time. Fourteen of 23 were newly discovered compounds, including aloinoside D, isoeleutherin, and ethylidene-aloenin, possessed antibacterial, anti-inflammatory, or free radical scavenging activities. The results of this study further enrich the information on the chemical constituents of the traditional Chinese medicine Aloe made from A. barbadensis, and lay a foundation for the study of the therapeutic material basis and quality control methods of Aloe.]]> 2024/3/3 12:56:19 1, HAO Beiquan¹, LI Yinqing², PI Guopei², XU Feng^1*, LIU Guangxue¹, SHANG Mingying¹, CAI Shaoqing¹]]> ZHANG Jing¹, HAO Beiquan¹, LI Yinqing², PI Guopei², XU Feng^1*, LIU Guangxue¹, SHANG Mingying¹, CAI Shaoqing¹ 13true Pieris japonica and their acetylcholinesterase(ACHE)inhibitory activities, with the aid of thin-layer chromatography color characteristics, silica gel, MCI and semi-preparative high-performance liquid chromatography technology, the target constituents were isolated and purified. The structures of the obtained compounds were identified by analyses of their spectral data(NMR and MS)and comparison of their data with those of reported in the literature. Meanwhile, AChE inhibitory effects of obtained diterpenoids were evaluated for the first time by a ellman method. The results showed that eight diterpenoid compounds were isolated and identified from the leaves of P. japonica, namely pieriformoside F(1), 3-epi-grayanotoxin XVIII(2), 3-epi-grayanotoxin B(3), asebotoxin-X(4), pieriformosin B(5), asebotoxin Ⅲ(6), rhodojaponin Ⅲ(7), and pieriformosin C(8). Among them, Compound 1 was isolated from this plant for the first time, and Compound 8 showed AChE inhibitory activity. In conclusion, the results enriches the diterpenoids and bioactive components of P. japonica, which provides a certain theoretical basis for its further development and utilization.]]> 2024/3/3 12:56:19 1,2, QUAN Wei³, LUO Ee², QIN Xujie², HUA Yan^1*]]> LI Huijuan^1,2, QUAN Wei³, LUO Ee², QIN Xujie², HUA Yan^1* 12true Amorphophallus konjac)is a horticultural plant with high nutritional and medicinal values. Soft rot is a severe disease in production of konjac and it is also the main factor restricting the development of the konjac industry. It has been reported that the soft rot of konjac is mainly caused by pathogenic bacteria(mainly including Pectobacterium aroidearum, P. carotovorum subsp. carotovorum, P. chrysanthemi and Enterobacter sp.), and there is rare reports on pathogenic fungi that cause konjac soft rot. In order to clarify the pathogenic types and infection characteristics of the soft rot in Qujing City, Yunnan Province, the diseased corms were collected for fungal isolation by tissue isolation methods. The isolated fungi were identified by morphological and molecular identification methods based on ITS and LSU sequence analyses, and pathogenicity was determined according to Koch's rule. The infection characteristic was analyzed by mixed inoculation using the identified pathogenic fungi and the pathogenic bacteria of konjac soft rot. The results were as follows:(1)Three species of Fusarium spp.(Fusarium concentricum, F. oxysporum and F. ambrosium), one species of Mucor sp., one species of Rhizopus sp., one species of Penicillium sp. and one species of Clonostachys sp. were identified.(2)Statistics analysis found that Fusarium concentricum had the highest relative abundance(45.45%).(3)Koch postulates tests showed that inoculation with F. concentricum caused obvious soft rot symptoms of konjac corms within three days.(4)In addition, mixed Pectobacterium aroidearum and Fusarium concentricum together inoculation promoted the disease development, and the weight of rotten tissue was significantly higher than that of single inoculation using F. concentricum or Pectobacterium aroidearum. Overall, these results indicate that konjac soft rot may be caused by a combination of fungus and bacterium infection. The results provide a theoretical reference for the prevention and management of konjac soft rot.]]> 2024/3/3 12:56:19 1, DONG Kun², ZHANG Yan'an¹, GAO Yong¹, CHEN Hong¹, FANG Pingping¹, LEI Hongxian¹, LU Xiaoqian¹, CHU Honglong^1,3*]]> LI Zhumei¹, DONG Kun², ZHANG Yan'an¹, GAO Yong¹, CHEN Hong¹, FANG Pingping¹, LEI Hongxian¹, LU Xiaoqian¹, CHU Honglong^1,3* 11true Bletilla striata and study the inhibiting effects of herbal extracts on pathogens, the pathogens that caused tuber rot of B. striata were isolated using usual tissue isolation the strains were identified by morphological and molecular biological techniques, and inhibiting effects of seven traditional Chinese medicine extracts on pathogen were observed. The results were as follows:(1)A total of fourteen fungi and four bacteria were isolated from diseased leaves, leaf sheaths and tubers. But only strain GF-1 caused disease, whose symptoms consistent with those in the field. The incidences of GF-1 disease reinoculated in the field and laboratory were both 100%.(2)GF-1 was identified as a member of Epicoccum, and its colonial morphology was a circular form, with white mycelium, prostrate on the medium, aerial, diaphragms and branches. There were conidia and chlamydospores.(3)At last, the sequence of internal transcribed spacer(ITS)region of GF-1 were analyzed, the length was 522 bp. The sequence was compared with other species in the GenBank and reached 99.62% similarity to Epicoccum sorghinum (MN493119.1)isolated form Sorghum, which was closer than others, including Epicoccum sorghinum(MF948994.1)isolated form leaves of Bletilla striata.(4)GF-1 could be fully inhibited when the medium contained 0.1-0.2 g·mL^-1 extracts that extracted from seven traditional Chinese medicines, respectively. It also could be fully inhibited by 0.05 g·mL^-1 of Cinnamomum cassia or Syringa oblate. In summary, the pathogen that caused tuber rot in Bletilla striata was identified as Epicoccum sorghinum. And GF-1 could be fully inhibited cultivated on the medium which contained 0.1-0.2 g·mL^-1 herbal extracts, e.g.: Cinnamomum cassia, Syringa oblate, Cyclocarya paliurus, Bletilla striata, Houpoea officinalis, Illicium verum or Cnidium monnieri. The results provide theoretical references for the control of tuber rot of Bletilla striata.]]> 2024/3/3 12:56:19 1,2, LU Xi^1,2, WU Qiaofen², YANG Yanni², XIA Ke², ZHAO Zhiguo², ZHENG Wenjun^1*, QIU Shuo^2*]]> MA Xiaoya^1,2, LU Xi^1,2, WU Qiaofen², YANG Yanni², XIA Ke², ZHAO Zhiguo², ZHENG Wenjun^1*, QIU Shuo^2* 10true Rubus suavissimus is mainly distributed in Guilin, Liuzhou, Wuzhou and other regions in Guangxi Zhuang Autonomous Region, so it is called as “Guangxi tiancha” in China. R. suavissimus, together with Siraitia grosvenorii and Stevia rebaudianastevia are praised as three famous sweet plants in Guangxi Zhuang Autonomous Region. Zhuang and Yao people use the leaves of R. suavissimus as a tea to treat diabetes, and it is known as the “divine tea” of Yao medicine. Therefore, R. suavissimus is a combination of sugar, tea and medicine, which has great potential for the development of food and medicine. In order to investigate the secondary metabolites with α-glucosidase inhibitory activity from R. suavissimus, herein, the extraction, separation and purification of secondary metabolites were performed on the leaves of R. suavissimus. The structures of purified compounds were determined based on the data of mass spectrometry(HR-ESI-MS)and nuclear magnetic resonance(¹H NMR and ¹³C NMR). In addition, the α-glucosidase inhibitory activity of the purified compounds were evaluated by pharmacological methods simultaneously. The results were as follows:(1)Ten compounds were purified and their structures were elucidated as rubusoside(1), kaempferol 3-O-robinobioside(2), gallic acid(3), dihydrodehydroconiferyl alcohol(4), 5-methoxydihydrodehydroconiferyl alcohol(5), brevifolincarboxylic acid(6), steviolmonoside(7), steviol(8), 16α, 17-dihydroxykaurane(9), and quercetin 3-O-β-D-galactopyranoside(10). Among them, compounds 2, 4, 5 and 9 were isolated from R. suavissimus for the first time.(2)Compounds 2, 3, 5, 6 and 10 showed strong inhibitory activity on α-glucosidase. The IC₅₀ values of compounds 2, 3, 5, 6 and 10 were(0.14 ± 0.03)mg·mL^-1,(0.36 ± 0.02)mg·mL^-1,(0.44 ± 0.01)mg·mL^-1,(0.53 ± 0.04)mg·mL^-1 and(0.14 ± 0.03)mg·mL^-1 respectively, which were stronger than the positive control acarbose with the IC₅₀ values as(0.69 ± 0.02)mg·mL^-1. Thus, compounds 2, 3, 5, 6 and 10, which were isolated from the leaves of R. suavissimus, could be a potential α-glucosidase inhibitors based on their bioactivity results. Compounds with α-glucosidase inhibitory activity from R. suavissimus will provide the basis for development of related hypoglycemic products.]]> 2024/3/3 12:56:19 *]]> YANG Bingyuan, HE Ruijie, WANG Yafeng, YAN Xiaojie, HUANG Yonglin^* 9true Acanthopanax senticosus bred by our team, its tender stems and leaves characterized with gray-purple color and sweet taste. This study is aim to isolate and purify the polysaccharides from the tender stems and leaves of ‘Zijia 1', and determine the monosaccharide composition and molecular weight of different fractions obtained after separation, and the antioxidant activity of each fraction was evaluated. The crude Asenticosus polysaccharides(ASPS)were obtained from the tender stems and leaves of ‘Zijia 1' by water extraction and alcohol precipitation, which were then separated and purified by DEAE-Cellulose 52 ion column and Sephadex G-100 gel column to obtain a uniform component. The ion chromatography and gel permeation chromatography-refractive index-multi-angle laser light scattering method was exploited to determine the monosaccharide compositions and molecular weight of the polysaccharides fractions. The hydroxyl radical(·OH), superoxide radical(O₂^-·)and 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)scavenging ability were determined to evaluate the antioxidant of each fraction in vitro. Four polysaccharides ASPA-1-1, ASPA-2-1, ASPA-3-1 and ASPN-1 were isolated and purified from ASPS, with molecular weight of 8.10, 26.15, 0.91, 0.89 kDa, respectively, mainly composed of arabinose, rhamnose, galactose, glucose, xylose, mannose, ribose, galacturonic acid and glucuronic acid in different proportions. The ASPA-1-1, ASPA-2-1, ASPA-3-1 and ASPN-1 from ‘Zijia 1' demonstrated significant scavenging activities on ·OH, O₂^-· and DPPH free radical, the ability of ASPA-2-1 to scavenge ·OH and DPPH is higher than ASPA-1-1, ASPA-3-1 and ASPN-1; ASPA-3-1 has the strongest ability to scavenge O₂^-·. Therefore, the purified polysaccharides from ‘Zijia 1' has obvious antioxidant activity, which provides a scientific theoretical basis for its further utilization.]]> 2024/3/3 12:56:19 *]]> ZHOU Jinyan, ZHANG Yun, LIU Liangyan, ZHANG Hui, RUAN Liuyang, ZENG Qianchun^* 8true Ardisia crenata var. bicolor is a common medicine used by Miao Minority in Guizhou, which has the effects of clearing throat and benefiting pharynx, reducing swelling and relieving pain, dispelling wind and dehumidifying. In order to study the chemical constituents and anti-inflammatory activities of A. crenata var. bicolor roots, the 70% ethanol extact was separated and purified by silica gel chromatography, Sephadex LH-20 gel column chromatography, ODS reverse column chromatography and semi-preparative HPLC. The structures of the compounds were identified by spectral data of NMR, MS and published literatures. Using lipopolysaccharide(LPS)-activated RAW 264.7 cell line model in vitro, compounds 1-4 were evaluated for the inhibition against nitric oxide(NO)production. The results were as follows:(1)Ten compounds were isolated from the 70% ethanol extract and identified as 11-O-galloylbergenin(1), 11-O-(4-O-methylgalloyl)bergenin(2), 11-O-vanilloylbergenin(3), 6-O-(4-hydroxy benzoyl)bergenin(4), 11-syringyl bergenin(5), 11-O-(3',4'-dimethylgalloyl)-bergenin(6), demethoxybergenin(7), micractinin A(8), monomethyl olivetol(9), and dibutyl phthalate(10). Among them, compounds 4, 8, 9 were obtained from Ardisia for the first time.(2)The results of anti-inflammatory activities in vitro showed that compounds 1-4 could significantly inhibit NO release in RAW 264.7 cells(P<0.01), and the inhibition rates of compounds at the concentration of 20 μmol·mL^-1 were 67.09%, 66.50%, 59.83%, 36.47%, respectively. This study enriches the chemical constituents of A. crenata var. bicolor roots, clarifies the material basis of its anti-inflammatory activities, verifies the scientificity of its traditional usage, and provides new insight and scientific evidence for its rational development and utilization of the medicinal resources.]]> 2024/3/3 12:56:19 *]]> YE Hongbo, ZHOU Yongqiang, LIAO Zhangrong, WEI Xin, YIN Xin, LI Jiaxin, ZHOU Ying^* 7true Camellia luteoflora, as well as their inhibitory effects on plant pathogenic fungi, this study used tissue separation method to isolate and purify endophytic fungi in C. luteoflora. These fungi were identified based on morphology and molecular biology, and their diversity was evaluated through statistical analysis. The strains with antibacterial activity were screened out by the plate confrontation method. The results were as follows:(1)A total of 261 strains of endophytic fungi were isolated from 324 C. luteoflora tissue samples, belonging to 1 phylum, 5 classes, 9 orders, and 22 genera. The dominant genera were Colletotrichum, Diaporthe and Pestalotiopsis with isolation frequencies of 21.84%, 16.86% and 10.34%, respectively.(2)The distribution of endophytic fungi in C. luteoflora varied in different seasons. The highest number of strains was isolated in winter(72 strains, accounting for 27.59%, belonging to 16 genera), 62 strains were isolated in spring(belonging to 13 genera), 59 strains were isolated in summer(belonging to 15 genera), and 68 strains were isolated in autumn(belonging to 13 genera). The Shannon-Wiener index(H'), Simpson index(D), Pielou's evenness index(E), and Margalef's richness index(M)were the highest in winter. The similarity of endophytic fungal species between spring and winter was higher, and the similarity between summer and autumn was higher.(3)The distribution of endophytic fungi also varied in different parts of C. luteoflora. The stems had the most abundant endophytic fungi, with 102 strains accounting for 39.08%(belonging to 15 genera), 61 strains were isolated from the roots(belonging to 10 genera), and 98 strains were isolated from the leaves(belonging to 15 genera). The H', D, E, and M were highest in the stems, and the endophytic fungal species in the leaves were most similar to those in the stems.(4)The plate confrontation results showed that among the 35 tested endophytic fungi, 26 endophytic fungi had inhibitory effects on at least one plant pathogenic fungi, accounting for 74.29%, among which CJ-Ⅱ-2, XY-V-3, QY-Ⅱ-4, QJ-Ⅲ-2 and DJ-I-2 had inhibitory effects on eight plant pathogen fungi to varying degrees. XY-V-3 had the best inhibitory effect on eight plant pathogenic fungi, and the inhibitory rate was higher than 50%. XY-V-3 and QJ-Ⅲ-2 have higher inhibitory rate than 50% on two strains of pathogenic fungi in C. luteoflora, which had the potential to control disease of C. luteoflora. To sum up, the diversity of endophytic fungi in C. luteoflora is rich, and some of the strains have higher effect of inhibiting plant pathogenic fungi, which lay a foundation for the research and development of biological control products and the disease control of C. luteoflora.]]> 2024/3/3 12:56:19 *]]> YI Hang, HE Jing, YANG Xi, RONG Shutian, WANG Li^* 6true Pteris ensiformis, silica gel, gel, MCI, C₁₈ and other column chromatography were used for separation and purification, and their structures were identified by ¹H-NMR, ¹³C-NMR, MS, IR and othe spectral data; the anti-tumor and anti-coagulation activities of some monomers were screened by MTS, APTT, PT and TT. The results were as follows:(1)A total of 15 compounds were isolated from P. ensiformis, the compounds were 2-hydroxy-acetylpyrrole(1), N-(3-carboxypropyl)-2-acetylpyrrole(2), 3-hydroxy-2-methylpyridine(3), N-methylhydroxylamine(4), pterosin S 13-O-glucoside(5), obtupterosin C(6), ent-11α-hydroxy-15-oxokauran-19-oic acid(7), ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid(8), β-sitosterol(9), ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid-O-glucopyranoside(10), 5, 5'-dibutoxy-2, 2'-bifuran(11), 5, 5'-di(2-ethyl-hexyloxy)-2, 2'-bifuran(12),(-)-loliolide(13), succinic acid(14), fumaric acid(15). Compound 1 is a new natural product of pyrrole alkaloids. Compounds 1-7, 10-15 were isolated from P. ensiformis for the first time, and compounds 1, 3, 4 were isolated from Pteris for the first time.(2)The results of activity test showed that compounds 1, 2, 3, 5, 6 and 10 inhibited the growth of tumor cells HL-60, A549, SMMC-7721, MDA-MB-231 and SW480 in vitro at a concentration of 40 μmol·L^-1, At the concentration of 2.0 mmol·L^-1, compounds 1, 2, 3 and 6 shortened APTT and compounds 1, 5 and 6 prolonged PT. The study enriches the chemical constituents of P. ensiformis from Guizhou and provides a material basis for the development of anti-tumor drugs.]]> 2024/3/3 12:56:19 1, SHEN Linyan¹, ZHANG Jingjie¹, HE Kang¹, YE Jianghai¹, ZHAO Chenliang¹, ZHANG Qilong², ZOU Juan^1*]]> XIAO Li¹, SHEN Linyan¹, ZHANG Jingjie¹, HE Kang¹, YE Jianghai¹, ZHAO Chenliang¹, ZHANG Qilong², ZOU Juan^1* 5true Ludisia discolor, gas chromatography-mass spectrometry(GC-MS)was carried out to analyze the chemical constituents of petroleum ether fraction of ethanol extracts. Meanwhile, the compounds were isolated from the ethyl acetate fraction by using various column chromatography including silica gel, Sephadex LH-20, and semi-preparative HPLC. MS and nuclear mgnetic resonance(NMR)spectroscopic analysis were used to identify the structure of the compounds. And then the isolated compounds were tested for anti-inflammatory with RAW 264.7 cells, and antioxidant activities were carried out with DPPH and ABTS·⁺ free radical scavenging ability. The results showed as follows:(1)A total of 17 compounds were identified from the petroleum ether fraction of L. discolor, in which, methyl palmitate, methyl linoleate, methyl linolenic acid, and 2-monopalmitin had a relatively high content.(2)A total of 15 compounds were isolated from the ethyl acetate fraction, their structures were identified as matairesinol(1),(+)-pinoresinol(2), callyspongidipeptide A(3), cyclo- [(S)-Pro-(R)-Leu](4), epi-boscialin(5), dibutyl terephthalate(6), methyl 4-hydroxycinnamate(7), 1-(4-hydroxyphenyl)ethanone(8), 4-hydroxybenzyl alcohol(9), p-hydroxy-benzaldehyde(10), vanillin(11), 4-(methoxym-ethyl)phenol(12), β-sitosterol(13), tetradecanoic acid(14), and palmitic acid(15), respectively. Compounds 1-15 were isolated from L. discolor for the first time.(3)Phenylpropanoid compounds 1 and 2 showed moderate inhibition on NO production from RAW264.7 cells induced by LPS, with IC₅₀ values of(37.76 ± 2.68)μmol·L^-1 and(53.14 ± 1.63)μmol·L^-1 [(quercetin as the positive control, with IC₅₀ value of(9.32 ± 0.36)μmol·L^-1], respectively. Antioxidant results showed that compounds 1 and 2 had moderate DPPH free radical scavenging ability, with IC₅₀ values of(51.22 ± 1.07)μg·mL^-1 and(79.22 ± 7.44)μg·mL^-1 [vitamin C as the positive control, with IC₅₀ value of(6.01±0.17)μg·mL^-1], respectively. Meanwhile, they had stronger ABTS·⁺ free radical scavenging ability than the positive control trolox [IC₅₀ =(34.65 ± 0.53)μg·mL^-1], with IC₅₀ values of(2.21 ± 0.01)μg·mL^-1 and(3.58 ± 0.17)μg·mL^-1, respectively. Overall, this study enriched the chemical constituents of L. discolor, and it was further clarified that phenolic compounds are the active antioxidant constituents in L. Discolor, and phenylpropanoid compounds have certain anti-inflammatory activities, which provides a chemical structure basis for subsequent in-depth pharmacological research.]]> 2025/1/7 10:23:14 1,2, CHEN Huiqin², WU Fei², MEI Wenli², ZHONG Yunfang¹, FENG Xueping^1*, DAI Haofu^2*]]> SHEN Ying^1,2, CHEN Huiqin², WU Fei², MEI Wenli², ZHONG Yunfang¹, FENG Xueping^1*, DAI Haofu^2* 4true Torricellia angulata var. intermedia belongs to the genus Torricellia. In China, this plant is mainly found in Guangxi, Guizhou, Yunnan and Sichuan. T. angulata var. intermedia has been prescribed for the treatment of various diseases in the field of traditional Chinese medicine, such as rheumatoid arthritis, closed soft-tissue injuries. In order to study the chemical constituents of the roots and rhizoma of T. angulata var. intermedia. This paper explored the chemical constituents of 45% ethanol extraction of T. angulata var. intermedia by D101 macroporous resin, silica gel, preparative high performance liquid chromatography and other methods. Their structures were elucidated by extensive spectroscopic analysis such as 1D-NMR, 2D-NMR, HR-ESI-MS and so on. The absolute configurations were determined by comparison of the experimental and theoretically calculated ECD. The results were as follows: Fifteen compounds were isolated and identified as(7S,8S)-8,9-dihydroxy-3,5,7-trimethox phenicol-4-O-β-D-glucoside(1), adenosine(2),(8E)-decaene-4,6-diyn-1-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(3), 2,6-dimethoxy-p-benzoquinone(4), palmiticacid(5), 2,2'-oxybis(1,4-di-tert-butylbenzene)(6), syringaldeyde-4-O-β-D-glucopyranoside(7), phytol(8), p-hydroxybenzaldehyde(9),(8R)-deca-2-trans-2-ene-4,6-diyn-1,8-diol(10), nicotinamide(11), aralidioside(12), β-daucosterol(13), griselinoside(14), 2-(4-hydroxy-3-methoxyphenyl)-3-(2-hydroxy-5-methoxyphenyl)-3-oxo-1-propanol(15). Among them, 1 was a new compound, 2-4, 6, 7, 9-12 and 15 were isolated from Toricellia for the first time. The results of this study enrich the phytochemical information of T. angulata var. intermedia, and provide a material basis for the elucidation of its pharmacodynamic substances, the search for quality markers, the exploration of pharmacological mechanisms and the development of drugs.]]> 2025/1/7 10:23:14 1,3, NIE Yushan^1,3, LI Xue^1,3, LIU Chunhua¹, LI Yueting², MA Xue¹ , LI Yongjun^1,2,3*]]> ZHU Banghui^1,3, NIE Yushan^1,3, LI Xue^1,3, LIU Chunhua¹, LI Yueting², MA Xue¹ , LI Yongjun^1,2,3* 3true Dendrobium chrysotoxum. In this study, solid-phase microextraction(SPME)combined with GC-MS was used to detect the volatile components of D. chrysotoxum in four periods: bud period, first-flowering period, full-flowering period and last-flowering period, and four parts of the labellum, sepals, petals and pistil column in full-flowering period, and on the basis of this analysis, we carried out the analysis of principal components and the pattern of fragrance release. The results were as follows:(1)A total of 29 volatile components were detected in the 4 periods of D. chrysotoxum, consisting of 13 alkenes, 6 alcohols, 2 aldehydes, 2 esters, 2 alkanes, 1 phenol, 1 acid and 1 other; a total of 39 volatile components were detected in the 4 parts of D. chrysotoxum during the full-flowering period, consisting of 15 alkenes, 9 alcohols, 4 alkanes, 3 esters, 3 ketones, 2 aldehydes, 2 others and 1 acid.(2)The volatile components and content in D. chrysotoxum gradually increase and then decrease during the whole process from full-flowering to decaying, and there are up to 22 volatile components in the full-flowering period, mainly alkenes and esters.(3)A total of 7 volatile components, namely, octyl acetate, ocimene,(+)-α-pinene,(-)-β-pinene, laurolene, d-limonene and 1-octanol, were important volatile components in D. chrysotoxum. Among them, octyl acetate and ocimene were the most released in the full-flowering period, which accounted for 31.74% and 24.98% of the total content, and the contents of acetic acid, octyl ester were detected in the three periods of bud, first-flowering and full-flowering, and the content was higher;(+)-α-pinene,(-)-β-pinene, myrcene, d-limonene and 1-octanol were detected only in the first-flowering and full-flowering periods and their contents increased gradually with the opening of the flowers.(4)The types and contents of volatile components released from different parts of D. chrysotoxum also varied. The main odoriferous parts were petals and sepals, with 29 types of volatile components detected in sepals, 20 types of volatile components in petals, with the highest total content of 5 754.26 ng·g^-1. In conclusion, this study shows that the volatile components released by different periods and different parts of D. chrysotoxum are different, and the results of this study lay a theoretical foundation for developing industry of daily chemical products of D. chrysotoxum flower.]]> 2025/1/7 10:23:14 1,2, CAO Hua², LU Lin², LI Limei³, SHEN Dingcai³, LI Han^2*, LI Donghui^1*]]> ZHAO Ruijing^1,2, CAO Hua², LU Lin², LI Limei³, SHEN Dingcai³, LI Han^2*, LI Donghui^1* 2true -1, followed by ‘Britewell' at 162 200.88 ng·g^-1; ‘Coastal' and ‘Powderblue' have lower total aroma component contents, at 91 284.45 ng·g^-1 and 97 511.10 ng·g^-1, respectively. The significant differences in aroma components and contents among these five rabbit-eye blueberry varieties provide an important basis for the selection of blueberry varieties and the choice of raw materials.]]> 2025/1/7 10:23:14 *]]> ZHAO Liangqing, NIE Fei, PENG Ge, WEN Guangqin^* 1true