Delphinium pachycentroides,of Ranunculaceae is described.]]> 2016/1/15 10:57:48 WANG Wen-cai 11true Viola principis H.Boissieu and V.pogonantha W.W.Sm.are reduced to new synonymies,V.kwangtungensis Melch.should treated as synonym to V.kiangsiensis W.Becker.]]> 2016/1/15 10:57:48 1,2, LI Chang-Han¹, XING Fu-Wu^1*]]> ZHOU Jin-Song^1,2, LI Chang-Han¹, XING Fu-Wu^1* 10true Rhododendron seniavinii Maxim.var.shangyounicum R.L.Liu et L.M.Cao,a new variety of the genus Rhododendron(Ericaceae)from Jiangxi,China,is described and illustrated in this paper.]]> 2016/1/15 10:57:48 *]]> CAO Li-Min, LIU Ren- Lin^* 9true Tongoloa Wolff in China were examined under SEM for the first time. The results indicate that the ornamentation variation of pericarp surfaces is obvious at interspecies level. Based on the comparison of pericarp surface characteristics of these species,rugose,rugulous-colliculate-rectangular and rugulous-irregular-tuberculate types are distinguished. Rugose and rugulous-colliculate-rectangular are all characterized by invisible outline of cell of the pericarp surfaces,but the type of ornamentation at the former is lack or sparseness striate,which shows lineate at the later. Rugulous-irregular-tuberculate appears the shape of cell of the pericarp surfaces,which is often subconical or polygonal with striate or reticulate ornamentation. These micromorphological features are evaluated as possible consistent parameters in the delimitation of species of Tongoloa Wolff.]]> 2016/1/15 10:57:48 1, LIU Qi-Xin^2*]]> PU Gao-Zhong¹, LIU Qi-Xin^2* 8true Limonium bicolor; Melilotus suaveolens; Artemisia arggi and Salsoa collina)were researched in order to understand the relation between anatomical structure of plants and drought-resistant and salt-tolerant characteristics by setting-up cutting slice and microscope observation. The results showed that: drought-resistant characteristics of Salsola collina was the strongest since its stoma density,stoma size,leaves area were the lowest and its epidermis hair density was more. Since stoma size,stoma density of Artemisia argyi were lower and its epidermis hair density was more,epidermis hair was longer, its drought-resistant characteristics was stronger. The epidermis hair density of Limonium bicolor was more,but epidemis hair was shorter,stoma density was more and stoma size was bigger. Therefore,its drought-resistant characteristic was weaker. The characteristic of M.suaveolens was the weakest because its stoma density,stoma size was higher,epidermis hair density was lower and epidermis hair was shorter. There were more exudation tissue,salt tentacle,exudation cavity in L.bicolor which was eduction salt plant and strongest salt-tolerant characteristic. The internal secretion tissue was more,such as exudation cavity in S.collina and with thinner wall cells and mucus cells. Therefore,S.collina was stronger salt-tolerant plant. There were more salt tentacles in M.suaveolens,which was eduction salt plant and stronger salt-tolerant characteristic. There was not any secretion tissue in Artemisia argyi. Therefore,S.collina may be used as biological ameliorant of salt soil.]]> 2016/1/15 10:57:48 1, Yuji SAKAI², YANG Yang¹, YANG Jing-Hui^1*, ZHU Wen-Bi¹, LIU Yan-Jun¹]]> ZHANG Wei-Yu¹, Yuji SAKAI², YANG Yang¹, YANG Jing-Hui^1*, ZHU Wen-Bi¹, LIU Yan-Jun¹ 7true Melanelia collectered from Xinjiang were studied by light microscope and scanning electron microscope. The chemical components were analysed by colour test(CT),micricrystal test(MCT)and thin layer chromatography(TLC),and main taxonomic characteristics were also summarized.]]> 2016/1/15 10:57:48 1, Adilijiang·Abdulla², Abdulla·Abbas^2*]]> WANG Yu-Liang¹, Adilijiang·Abdulla², Abdulla·Abbas^2* 6true 2016/1/15 10:57:48 1, HUANG Jiang-Liu¹, LUO Mu-Lin², LI Gui-Fen¹, PENG Hong-Xiang¹]]> ZHU Jian-Hua¹, HUANG Jiang-Liu¹, LUO Mu-Lin², LI Gui-Fen¹, PENG Hong-Xiang¹ 5true Limnophila sessiliflora was cultivated in water medium polluted with Cd²⁺ of different gradient concentration for 48 h. The ultrastructural damages of leaf cells were observed by using transmission electron microscopy. The results showed:The higher concentration of Cd²⁺,the more serious the damage to ultrastructure. Ribosomes reduced,golgi apparatus slacked or disappeared,mitochondria ruptured or budded,plasma membrane contracted and plasmolysis took place,plasmodesma snapped,chloroplasts swelled and thylakoids disintegrated. These affected the function of cells in all and finally led to the cell damaged or destroyed.]]> 2016/1/15 10:57:48 ZHAO Chun 4true Zingiber:Z.xishuangbannaense 2n=22,Z.recurvatum 2n=22,Z.orbiculatum 2n=22,Z.zerumbet 2n=22,Z.purpureum 2n=22;(2)4 species of Globba:G.racemore 2n=24,G.marantina 2n=48,G.schomburgkii 2n=48,G.lancangensis 2n=32;(3)1 species of Boesenbergia:B.albomaculata 2n=36. The chromosome numbers of Z.recurvatum,Z.orbiculatum,Z.purpureum,G.lancangensis,B.albomaculata were reported for the first time.]]> 2016/1/15 10:57:48 *]]> LI Wei-Xiu, CHEN Jin^* 3true Salvia miltiorrhiza in four factors(the concentration of dNTP,Primers,Mg²⁺ and Taq DNA polymerase)at three levels respectively. Then,based on the optimal ISSR-PCR amplification system,the concentration of template DNA and annealing temperature were proposed by gradient PCR. The results showed that: a suitable ISSR-PCR amplification system of S.miltiorrhiza was established. In a total volume of 20 μL ISSR-PCR amplification system,it contains 1×PCR buffer,200 μmol/L dNTP,1.0 μmol/L primer,1.5 mmol/L Mg²⁺,1 U Taq DNA polymerase and 20～60 ng template DNA. The optimal annealing temperature for primer UBC 835 is 51.7 ℃.]]> 2016/1/15 10:57:49 LI Rong, WANG Zhe-Zhi 2true Hedychium was optimized including the concentration of Mg²⁺,dNTPs,TaqE,primers and DNA template etc. Compared with original system,this system reduced notably the consumption of all components except Mg²⁺. Amplified reactions of other fourteen genera in Zingiberaceae with this system appeared clear bands and high polymorphism. The result showed that SRAP maker can be used in taxonomy and genetic connection analysis of varieties,species or genera in Zingiberaceae.]]> 2016/1/15 10:57:49 1,3, LIU Nian^2*, HUANG Bang-Hai³, ZHANG Wei⁴]]> GAO Li-Xia^1,3, LIU Nian^2*, HUANG Bang-Hai³, ZHANG Wei⁴ 1true