Eucalyptus have not been demonstrated. In this study, adventitious root induction, growth of shoots and histochemical staining of basal stem were analyzed by adding the exogenous brassinosteriod(Concentration: 0, 0.005, 0.01, 0.05, 0.10, 0.20 mg·L^-1,respectively)in the root inducing medium of E. grandis clones EG5 and GL1. The results showed that the height of shoot and induction frequency of adventitious root of E. grandis clone EG5 were significantly affected by adding the exogenous brassinosteriod. Induction frenquency of adventitious root was 76.6%. The length of adventitious root of E. grandis clone EG5 was the highest in the root inducing medium containing 0.005 mg·L^-1 brassinosteriod for. In the meantime. The height of shoot decreased significantly with the increasing concentration of exogenous brassinosteriod and it was reduced by 21.8% at 0.10 mg·L^-1 brassinosteriod concentration compared to control. The adventitious root induction frequency of E. grandis clone GL1 reached the highest level with 88.3% in the root inducing medium of adding 0.05 mg·L^-1 brassinosteriod concentration but the height of shoot showed no significant change by adding the exogenous brassinosteriod. It was interesting that the root elongation of E. grandis clone EG5 was significantly inhibited due to the addition of the exogenous brassinosteriod. Root length and number were reduced to 2.49 cm and 1.75 at 0.20 mg·L^-1 brassinosteriod from 4.64 cm and 3.02(control)respectively. However, the lateral root induction and differentiation were not affected by adding the exogenous brassinosteriod. Besides, the growth in xylary cells of basal stem was clearly promoted and the cambium cell number of basal stem increased at 0.10 mg·L^-1 brassinosteriod by way of the histochemical analysis. Together, brassinosteriods was necessary to adventitious root induction and shoot growth and increasing xylem was a primer cause of lower frenquency of adventitious root induction at a few extra brassinosteriod.]]> 2016/8/18 11:42:35 *, LIU Ying, QIU Zhen-Fei, LI Xiang-Yang]]> YAN Hui-Fang, ZENG Bing-Shan, FAN Chun-Jie^*, LIU Ying, QIU Zhen-Fei, LI Xiang-Yang 5true Eucalyptus urophylla&#215;E. grandis(DH_-3229)plantation in Guangdong, three kinds of soil preparation(no controlled burning and dig hole, no controlled burning and furrowing, controlled burning and full cultivation)mode were selected, field investigation and indoor analysis methods were adopted to measure the carbon content in different organs. The main results were as follow:(1)The average carbon content in different organs of DH_-3229 was 44.37%-57.42%, and the carbon content sequence was leaf > truck > branch > root > bark. No significant difference(P<0.05)was observed in carbon content among the shrub, herb and litter layers of the DH_-3229 plantations with different soil preparation measures. Carbon content in the soil layer(0-100 cm)decreased with the increase of soil depth.(2)The carbon storage of no controlled burning and dig hole, no controlled burning and furrowing and controlled burning and full cultivation in tree layer were 18.01, 30.49 and 23.56 t·hm^-2. Carbon storage in various organs showed the order: stem > root > leaf > branch > bark. Except for the bark, the order of carbon storage in other organs under three different soil preparation measures was furrowing soil preparation > all reclamation soil preparation > dig hole soil preparation.(3)The total ecosystem carbon storage of DH_-3229 plantations under different soil preparation measures was 144.77 t·hm^-2 (dig hole), 197.03 t·hm^-2(burning)and 161.16 t·hm^-2(all reclamation soil preparation), and the carbon storage or der of different soil preparation measures in different layers appeared: soil layer>vegetable layer>litter layer. Carbon storage allocations in tree and soil layer of furrowing soil preparation were significantly larger than all reclamation soil preparation and dig hole soil preparation. The carbon storages of tree stratum and soil layer were the dominant part in carbon storage of the whole ecological system, which accounted for more than 93% of the carbon storage in the ecosystem. However, different combinations of measures on soil preparation have no significant effects on C storage allocations in the litter layer. Therefore, it appears furrowing soil preparation may be a more appropriate method to improve carbon storage of the DH_-3229 in the Leizhou Peninsula or other areas with similar site conditions.]]> 2016/8/18 0:00:00 *]]> LIU Guo-Cui, CHEN Shao-Xiong, WANG Zhi-Chao, ZHANG Li-Li, DU A-Peng^* 4true Eucalyptus urophylla &#215; E. grandis plantations have been planted in the southern area of China, especially in Guangxi Zhuang Autonomous Region in the recent years. The influence of water use efficiency in sustainable forest development and water resources management has been receiving increasing attention. Therefore, understanding the water use characteristics is important. The Granier thermal dissipation probe method(TDP)is commonly used to monitor tree trunk flow. The sap flow density(SFD)was monitored in four years E. urophylla &#215; E. grandis plantation for one and a half years by Granier TDP at Guangxi Huangmian forest. The change in regularity of SFD, the SFD in trees of different diameters at breast height and the relationship between the SFD and the environmental factors were studied. The results showed that average daily SFD was 830.1 L·m^-2·d^-1, and the maximum value did not exceed 2 000 L·m^-2·d^-1, when compared with similar studies, these results were relatively low. Because tree trunk was the main part of the plant for water transport, it was necessary to analyze the tree trunk sap flow density(SFD)change at about different diameters at breast height of E. urophylla &#215; E. grandis. There was a positive correlation between SFD and diameter. The SFD was similar when the DBH(diameter at breast height)were in the same diameter class. The SFD varied from tree to tree with different values of maximum and minimum SFD(1 300 L·m^-2·d^-1at most). This was mainly caused by the different water absorbing capacities of trees. Many studies showed that photosynthetic active radiation and vapor pressure deficit(VPD)were the main factors of tree canopy transpiration. The SFD and VPD, photosynthetic active radiation(PAR)displayed similar trend over the study period and exhibited a unimodal curve. The value of SFD was higher in summer and fall, and lower in spring and winter. The relation between SFD and PAR showed significant difference, and there was relation between SFD and VPD to some extent, SFD and air temperature(AT)and SFD and soil temperature(ST). But the relation between SFD and air relative humidity(RH), SFD and soil moisture(SM)were not clear. These results showed that there were different patterns about the effects of different environmental factors on the sap flow density, but further experimental verification is needed how to further explore the E. urophylla &#215; E. grandis respond to the environmental changes.]]> 2016/8/18 0:00:00 1, HUANG Yu-Qing^1*, GU Da-Xing¹, ZHAO Ping², ZHANG De-Nan¹, YAO Yue-Feng¹]]> ZHOU Cui-Ming¹, HUANG Yu-Qing^1*, GU Da-Xing¹, ZHAO Ping², ZHANG De-Nan¹, YAO Yue-Feng¹ 3true Eucalyptus, coniferous and broad-leaved tree species were studied at Lingtian Village of Guilin City, Guangxi Zhuang Autonomous Region. The results showed that the physiological and biochemical norms changed according to seasons in Eucalyptus and other four tree species. The SLA of five species was the highest in the summer and lowest in winter. The average SLA values were Eucalyptus > Castanopsis hystrix > C. fissa > Cunninghamia lanceolata > Pinus massoniana. Leaf chlorophyll of seasonal changes was as follows: summer > autumn > spring > winter. The average of total chlorophyll in four seasons were: Eucalyptus > Castanopsis fissa > C. hystrix > Cunninghamia lanceolata > Pinus massoniana. The SS with seasonal changes were: summer > spring > winter > autumn; the SP contents of the five species were the highest in spring and the lowest in summer, and the SP in coniferous were much higher than the broad-leaved species. The average SS and SP were the highest in Eucalyptus. Seasonal trends showed arched leaf RNA variation, the order of change was summer > spring > autumn > winter, with average maximum NRA in Eucalyptus. To sum up, in a natural environment for the growth, physiological and biochemical indices of these five species response to season were different, but the physiological indicators of Eucalyptus were generally higher than other species, which inflected that the artificial Eucalyptus forest had high productivity and growth advantage.]]> 2016/8/18 11:06:30 1,3, LIANG Shi-Chu^2,3, ZENG Xiao-Biao¹, HUANG Ya-Li²]]> SHI Gui-Yu^1,3, LIANG Shi-Chu^2,3, ZENG Xiao-Biao¹, HUANG Ya-Li² 2true Eucalyptus is one of the raw materials for forest bio-energy industry. Calorific value and ash content were two important indices for rational use of bio-energy Eucalyptus forest. Calorific values and ash contents of leaves, branches, roots, stem-wood and bark of 10 Eucalyptus species including Eucalyptus urophylla &#215; E. grandis in South China were determined by using calorimeter and muffle furnace. The results showed that gross calorific values(GCV)and ash free calorific values(AFCV)of different organs ranged from 15.10 to 21.06 kJ·g^-1 and 16.05 to 22.11 kJ·g^-1 respectively, leaves had the highest mean GCV and AFCV(19.50 kJ·g^-1and 20.56 kJ·g^-1)and bark the lowest(17.32 and 18.09 kJ·g^-1), which indicated that leaf had higher organic matters than other organs. The orders of decreasing GCV and AFCV of 5 organs in 10 Eucalyptus species werEucalyptus, calorific value, ash content, bio-energy, South Chinae not exactly the same. The ash contents of different organs ranged from 0.14% to 8.5% with leaves having the highest mean value(5.13%)and stem-wood the lowest(0.30%),which indicated that leaf had more mineral elements than other organs. There were significant differences in calorific values and ash contents of 5 organs(P<0.05). As to the whole tree, E. urophylla had the highest GCV and AFCV(18.99 and 19.18 kJ·g^-1), while Corymbia torelliana had the lowest GCV and AFCV(17.53 and 17.86 kJ·g^-1); C. torelliana had the highest ash contents(1.9%)and Eucalyptus grandis the lowest(0.61%). The results of correlation analyses showed that ash content had negative correlation with GCV and AFCV,which was not significant; GCV and AFCV had significantly positive correlation(P<0.01). For ideal plant fuel should have the characteristics of high calorific value and low ash content, of 10 species, Corymbia torelliana had low calorific values and high ash contents, which indicated that it was not ideal fuel species while Eucalyptus urophylla was the optimal species.]]> 2016/8/18 11:06:30 1*, CHEN Shao-Xiong¹, HAN Fei-Yang²]]> ZHOU Qun-Ying^1*, CHEN Shao-Xiong¹, HAN Fei-Yang² 1true