2016/8/18 11:06:30 1, YU Xin-Fan², ZHAO Kang-Sai², YANG Li-Jun^2*, LIANG Hong², LU Yun-He³]]> HUANG Yu-Yuan¹, YU Xin-Fan², ZHAO Kang-Sai², YANG Li-Jun^2*, LIANG Hong², LU Yun-He³ 6true Camellia oleifera)is the important oiltree in our country with the vastest area of distribution and the most products in the genus of Camellia. Guangxi Zhuang Autonomous Region, the important distribution area of oil tea, abounds in oil tea germplasm resources. In order to evaluate genetic variation of germplasm resources of oil tea in Guangxi for germplasm conservation and cultivar selection, 10 SSR primer pairs were screened and evaluated in the present study, then genetic diversity of 97 representive germplasm resources of oil tea in Guangxi was analysed based on the polymorphic SSR markers. The findings indicated as follows: Firstly, of the ten developed SSR primer pairs, seven codominant primer pairs could produced stable amplicons, and another two primers could produce unstable amplicons; the remaining one yielded no band on the gel. Secondly, a total of 33 alleles were detected among 97 oil tea germplasm resources using seven codominant SSRs, and the number of alleles was from three to six with an average of 4.714 3 for every pair of primers; the number of effective allele ranged from 2.084 2 to 4.314 8 with an average of 2.828 8, and the gene diversity was from 0.520 2 to 0.768 2 with an average of 0.628 1. Thirdly, almost loci out of Hardy-Weinberg equilibrium meant genetic structure in the population, the ranges of observed and expected heterozygosity were 0.413 0-0.670 1 and 0.523 3-0.772 4, with the averages of 0.569 8 and 0.631 6. Finally, the range of genetic distances among germplasm resources was 0.05-0.791 7, with an average of 0.354 5. The cluster analysis based on UPGMA method revealed that the germplasm resources from the same places could not cluster in the same branch, on the other hand, the germplasm resources in one branch came from several places. The foregoing results reveavled that the developed SSRs of oil tea were suitable to work in the oil tea in Guangxi and the genetic diversity of germplasm resources in Guangxi was relatively higher. Those findings provide the scientific evidences for deep exploitation and efficient utilization of genetic resources of oil tea in Guangxi.]]> 2016/8/18 11:06:30 1, WANG Xiao-Yun¹, QIN Zi-Hai², ZHAO Wen-Dong³, WEI Chang-Jiang³, WANG Peng-Liang^2*]]> ZHANG En-Hui¹, WANG Xiao-Yun¹, QIN Zi-Hai², ZHAO Wen-Dong³, WEI Chang-Jiang³, WANG Peng-Liang^2* 5true Flemingia macrophylla was estimated using ISSR method. An high level of genetic diversity was detected in wild populations of F. macrophylla from South Yunnan Province. At species level, the percentage of polymorphic loci PPL = 94.85%, effective number of alleles Ne = 1.462 7, Nei's gene diversity He = 0.281 5, and Shannon's information index Ho = 0.433 7; at population level, PPL = 43.44%, Ne = 1.298 1, Nei's gene diversity He = 0.170 4, and Shannon's in formation index Ho = 0.249 9. The level of genetic differentiation(39.75%)among populations is lower than that within populations(60.25%). Based on the results, we suggest that the protected areas of F. macrophylla should be build in MY, QB and NE because of the high level of genetic diversity.]]> 2016/8/18 11:06:30 *, XIAO Wen-Xiang, TANG Shou-Xian]]> WANG Gui-Juan^*, XIAO Wen-Xiang, TANG Shou-Xian 4true Angiopteris hokouensis populations collected from different habitats were analyzed by means of intersimple sequence repeat(ISSR)markers, and 8 proper primers with rich polymorphism and stable bands were selected from a total of 44 ISSR's primers. The genome DNAs of the 6 populations were amplified to a total of 144 putative bands by the 8 primers, in which 96 were polymorphism bands. Polymorphism rate was 93.7%; Nei's gene diversity index was 0.296, Shannon information index was 0.457, and Gst was 0.152 0. The genetic distance coefficient and the genetic similarity were 0.058 4-0.090 1 and 0.913 8-0.954 8. Cluster analysis by UPGMA indicated that the genetic distance between the populations related to spatial distance, as well as to habitats. The results showed that A. hokouensis populations had high levels of genetic diversity at disturbed habitats. Even there was no significant differentiation of genetic diversity among populations,the populations in moderate disturbed habitats had higher genetic diversity. The genetic diversity was not significantly related toaltitude,slope and aspect.]]> 2016/8/18 11:06:30 1, YANG Bin¹, HE Zhao-Rong², WANG Chong-Yun^1*, ZHANG Zi-Di², LIU Wei³]]> ZHU Xiao-Yuan¹, YANG Bin¹, HE Zhao-Rong², WANG Chong-Yun^1*, ZHANG Zi-Di², LIU Wei³ 3true Alternanthera philoxeroides, Solidago canadensis and Dysphania ambrosioides, total three invasive species were found in four habitats, and their important values were so low that they could not constitute a significant impact on native species. By the research it implied that different land use types seriously affected the species composition and species diversity of herb community. The herbaceous community changed with land use types variation, diversification of land use could contribute native herbaceous species diversity protection. Hence, to study on the herbaceous plant diversity of different land use patterns in rural environment is helpful for plant diversity conservation in rural area and pushing up beautiful China construction.]]> 2016/8/18 11:06:30 *, CHEN Guang-Cai, WANG Qing-Bing, WANG Li, ZHANG Ying]]> WU Hao, ZHANG Jian-Feng^*, CHEN Guang-Cai, WANG Qing-Bing, WANG Li, ZHANG Ying 2true Bletilla striata, as atraditional Chinese herbal medicine, is a terrestrial and perennial medicinal plant. It is necessary to obtain the culturable endophytic fungi in the application of mycorrhizal technology for the protection and artificial cultivation of B. striata. The independent fungal isolates were isolated from the roots and leaves of B. striata, and identified based on the fungal morphological characteristics and the analysis of the fungal internal transcribed spacer(ITS)of rDNA sequences. The results showed that thirty-seven independent fungal isolates were obtained from forty-five root tissues and forty-five leaf tissues of two wild plants of B. striata. The isolates were identified to 15 taxa, belonging to nine genera,eight families, seven orders, four classes, and two phyla. The four classes are corresponding to Leotiomycetes, Dothideomycetes, Sordariomycetes and Agaricomycetes. Twelve endophytic fungi were isolated from the roots, and Sebacina were dominant genus. Three endophytic fungi were isolated from the leaves, and Colletotrichum were dominant genus. The relative abundance of Sebacina or Colletotrichum was 20%. Four basidiomycetes all were not isolated from the leavf tissues but the roots. The diversity index of endophytic fungi in roots(H'=1.863)was higher than that in leaves(H'=1.098). The positive results in this report demonstrated that fungi isolated from B. striata could be used for the protection and artificial cultivation of the host. Moreover, these Basidiomycetous fungi simultaneously clould be used as a valuable candidate sources for the protection and sustainable utilization of orchid resources by mycorrhizal technology.]]> 2016/8/18 11:06:30 *]]> WEI Yan-Mei, ZHOU Ya-Qin, LI Li, TAN Xiao-Ming^* 1true