Camellia sect. Chrysantha are world-famous economic and ornamental plants with high ornamental and medicinal values. In order to determine the cold tolerance, to lay a theoretical foundation for cold tolerance breeding and to provide a technical support for artificial cultivation of Camellia sect. Chrysantha, two-year old leaves of five species were employed as materials and treated under artificially simulated low temperature environment, including C. nitidissima, C. longzhouensis, C. limonia, C. multipetala and C. tunghinensis. We obtained the changes of electrolyte leakage rates(REC)at 20 ℃(normal temperature control), 8 ℃, -2 ℃, -7 ℃, -12 ℃, -17 ℃, -22 ℃ and -27 ℃ by using conductance method,calculated the semi-lethal low temperatures(LT₅₀)based on the changes in electrolyte leakage rates(REC)under different low temperature stresses with the Logistic equation, and also analyzed the contents of free proline, soluble sugar and malondialdehyde(MDA)in leaves. The results showed that the semi-lethal temperature(LT₅₀)of five species was: C. nitidissima -14.58 ℃, C. longzhouensis -14.27 ℃, C. limonia -13.44 ℃, C. multipetala -13.09 ℃, and C. tunghinensis -12.74 ℃. The cold tolerance of five species Camellia sect. Chrysantha could be reliably reflected by the semi-lethal temperature. C. nitidissima and C. longzhouensis had high cold tolerance. C. limonia and C. multipetala had medium cold tolerance. C. tunghinensis had poor cold tolerance. The REC of the five species increased following an S-curve with the temperature drop, and temperature showed highly significant negative correlation to cold tolerance. The contents of free proline, soluble protein and MDA increased first and then decreased. Under the same temperature treatment, C. nitidissima with lower LT₅₀ temperature had higher contents of free proline, soluble protein and lower contents of MDA. The results provide the information for selection of cold-tolerant germplasm of Camellia sect. Chrysantha.]]> 2016/12/26 11:22:52 *]]> LI Ji-Tao, XIE Wei-Ling, CHAI Sheng-Feng, TANG Jian-Min, WEI Xiao^* 4true Camellia sinensis show that light and medium components had significant effects on metabolism of catechins in materials cultured in vitro and that DFR gene, LAR gene and PPO gene all have close relationship with it. For further study on molecular mechanism of metabolism of catechins and providing theoretical guidance for the deep development of catechins in C. nitidissima, calli rich in catechins were used as materials to study the variation of DFR gene expression, LAR gene expression, PPO gene expression and content of catechins and the correlation with each other in calli under different light source, hormones, carbon source or PHE treatments for 30 d. The results showed that all the above four detecting items reacted to in vitro treatments significantly. Under the above treatments, the expression pattern of DFR gene and LAR gene was very similar. The correlation coefficients between the two under these treatments were between 0.710 and 0.889. The correlation of PPO gene expression and content of catechins was significantly negative under different carbon source treatments and their correlation coefficient was -0.696. DFR gene expression was significantly positively related to the content of catechins under different PHE adding quantity treatments and the correlation coefficient was 0.786. LAR gene expression was also significantly positively related to the content of catechins under different PHE adding quantity treatments and the correlation coefficient was 0.564. MS solid medium supplented with 4 mg·L^-1 6-BA, 0.6 mg·L^-1 2,4-D, 30 g·L^-1 sucrose and 0.660 8 g·L^-1 PHE was suitable for in vitro production of catechins. Content of catechins in calli cultured in this medium for 30 d was about 40.11 mg·g^-1 DW. Based on the above research we concluded that similar to C. sinensis there was a close connection between DFR gene and LAR gene during metabolism of catechins in C. nitidissima and that the increase of expression of PPO gene caused the loss of catechins in C. nitidissima and that it was an effective way to add suitable amount of PHE for the increase of content of catechins in calli of C. nitidissima.]]> 2016/12/26 11:22:52 *]]> ZHONG Chun-Shui, LAI Rui-Lian, LIU Sheng-Cai, LAI Zhong-Xiong^* 3true Camellia is one of the world treasure plant resources. In this paper, eleven elements including Mg, Ca, Mn, Fe, Zn, Ni, Se, Pb, Cd, Hg and As were determined in leaves and corresponding soils of four yellow flower Camellia by atomic absorption spectrometry and atomic fluorescence spectrometry, and then the enrichment factors of these metal elements were calculated. The results were as follows:(1)The leaves of four yellow flower Camellia were rich in Mg, Ca, Mn, Fe, Zn, Ni and element contents were Ca > Mg > Mn > Fe > Zn > Ni > Se, while the toxic heavy elements such as Pb, Cd, As and Hg were low, which reached the standards of non-polluted tea.(2)There were obvious differences in metal element contents between young and old leaves. The concentrations of Ca, Mn, Fe, Zn, Pb, Cd, Hg, As and Se in old leaves were higher than those in young leaves, especially in Ca, Mn and Fe, while concentrations of Mg and Ni in young leaves were higher than those in old leaves.(3)Yellow flower Camellia showed different enrichment abilities to different metal elements. The enrichment factors were Ca, Mn, Mg > Zn, Ni, Hg > Pb, Se > Fe, As. Also, the enrichment characteristics were different in young and old leaves.(4)The enrichment abilities of four yellow flower Camellias were different. Overall, C. longzhouensis and C. murauchii had higher enrichment factors of Mg, Ca, Mn, Zn, Ni, Se and Pb than that of C. nitidissima and C. ptilosperma. In detail, C. longzhouensis and C. murauchii showed the highest enrichment of Mg, Mn, Se and Ca, Pb and Hg, respectively. C. nitidissima showed higher enrichment of Hg and lower enrichment of other elements, while C. ptilosperma had the lowest enrichment factors of Ca, Mn, Ni and Zn. The above results showed that plant species, parts and element type had a comprehensive effect on the enrichment factors. The results provide the information for reasonable development and utilization of yellow flower Camellia.]]> 2016/12/26 11:22:52 1,2, SHI Peng-Tao ^1,2, YU Yan-Ping¹, WANG Yun-Ru^1,2, JIANG Yue-Hua^1,2, NONG Yao-Jing^1,2*]]> QIN Yu-Yan^1,2, SHI Peng-Tao ^1,2, YU Yan-Ping¹, WANG Yun-Ru^1,2, JIANG Yue-Hua^1,2, NONG Yao-Jing^1,2* 2true Chrysantha were examined using the scanning electron microscope, including Camellia pingguoensis, C. multipetala var. patens, C. chrysantha. The characteristics of pollen morphology among the species were distinguished using data statistics and photographic analysis. The pollens were large pollen grains, mean polar axis pollen size in the three species was over 50 μm, blunt concave triangle in polar view. According to morphological photography, the aperture were three hole groove, namely N₃P₄C₅ type. Two basic sculpes were defined: tuberculate-baculate, viz, ornate. C. pingguoensis, C. multipetala var. patens were the latter, C. chrysantha was the other. C. pingguoensis relatively dense mesh, mesh ridge slightly rough, C. multipetala var. patens net ridge slightly uplift, C. chrysantha short strip projections. Pollen morphology has important significance for its classification, through the pollen exine sculpture type, C. chrysantha with C. pingguoensis, C. multipetala var. patens were more distant relatives. Pollen morphology had variability among species, had conservation within species, but the classification of C. pingguoensis and C. multipetala var. patens still need further study. By comparing the pollen size, shape, the exine pore characteristics and morphological indexes etc, the results showed that there was an obvious similarities and differences of three species of sect. Chrysantha pollen morphology but sect. chrysantha set classification is complex, not only from the palynology into consideration, has a long way for sect. Chrysantha set classification. Sect. Chrysantha found up to now, enjoyed a good reputation at home and abroad, has been enjoying by botanists, horticulturalist, but its classification system is not yet clear. In fact, classification system has been being improved, sect. Chrysanthae has ornamental value and highly potential value. The results provide sect. chrysanthae germplasm resources classification and the use of value, promote sect. Chrysanthae development of resource utilization.]]> 2016/12/26 11:22:52 1, ZHA Qian-Hui¹, HUANG Yong-Fang^1*, HONG Wen-Hong¹, XUE Ke-Na²]]> TAN Sha¹, ZHA Qian-Hui¹, HUANG Yong-Fang^1*, HONG Wen-Hong¹, XUE Ke-Na² 1true