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<title cf:type="text"><![CDATA[ -->Special Column：Genetic Diversity]]></title>
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<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparative analysis on the genetic diversity of 41 Vitis 
germplasm resources by ISSR and SCoT molecular markers]]></title>
<link><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170101&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Seventeen wild germplasm resources of Vitis heyneana collected from Guilin City of Guangxi Zhuang Autonomous Region and 24 grape cultivars were used to study their genetic diversity and relationship using twelve ISSR and twelve SCoT markers. The results showed that both the two types of molecular markers produced rich polymorphic fragments, and could be used on the genetic diversity study of Vitis. However, the results of cluster analysis showed the difference between ISSR and SCoT, and the SCoT markers rather than ISSR markers were much better at distinguishing the wild and the cultivars of Vitis, therefore, the SCoT markers might have more advantages on the study of genetic diversity and phylogenetic analysis of Vitis. The clustering results of SCoT showed that the three wild V. heynean “zws1”, ”zws2” and “zws3” collected from Guangxi Institute of Botany grouped together with cultivars rather than the other wild ones, which showed that maybe one of the ancestral parents of these cultivars was derived from this category of wild V. heyneana. All the seventeen wild germplasms clustered into different groups and showed distinct regional character, nonetheless, the genetic distance was long between different groups, which showed that the wild V. heyneana had abundant genetic diversity in Guilin. The cultivars did not show obvious clustering characteristics due to the fact that most of them had no regional representativeness or their genetic background were nearly equal due to constantly interbreeding, resulting in low genetic diversity. The findings proved that the SCoT molecular markers had more advantages in the genetic diversity study of Vitis. The findings provides the information for the conservation and utilization and variety breeding of wild V. heyneana in Guilin.]]></description>
<pubDate>2017/2/9 11:03:17</pubDate>
<category><![CDATA[Special Column：Genetic Diversity]]></category>
<author><![CDATA[WANG FaMing, LI JieWei*, YE KaiYu, GONG HongJuan, 
MO QuanHui, JIANG QiaoSheng, LIU PingPing]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG FaMing, LI JieWei*, YE KaiYu, GONG HongJuan, 
MO QuanHui, JIANG QiaoSheng, LIU PingPing</atom:name>
</atom:author>
<guid><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170101&flag=1]]></guid><cfi:id>7</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Genetic diversity and relationship of endangered 
plant Heteroplexis microcephala assessed 
with ISSR polymorphisms]]></title>
<link><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170102&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Heteroplexis microcephala is an endangered plant only found in karst limestone regions in the Yangshuo County of Guangxi Zhuang Autonomous Region in China. In the present study, ISSR markers were used to evaluate the genetic variation within and among wild populations that were sampled from Guangxi with a goal to collect basic genetic information for its conservation. Leaf samples of 141 individuals were collected from six counties. Based on 10 ISSR primers, 96 clear and reproducible DNA fragments were generated. The percentage of polymorphic bands (PPB) was 30.21%, Nei’s gene diversity (H) was 0.105 4, Shannon information index (I) was 0.154 6 at the species level, and PPB among population ranged from 9% to 19%, H ranged from 0.021 2 to 0.051 3, I ranged from 0.033 9 to 0.080 5. High levels of genetic differentiation among the populations (Gst=0.690 5) were detected on the basis of results from POPGENE, which indicated that 69.05% of the genetic variability was distributed among populations, and only 30.95% of the variation existed within populations. Molecular variance was also examined using AMOVA based on RAPD banding patterns. The variance component found within populations was 27.28%, and a variance of 72.72% was found among populations.  Gene flow(Nm) among the population was 0.224 2 indicating that there was a low migration rate between populations. The highest genetic distance based on Nei’s genetic genetic distance for all population pairs was 0.160 9 between populations BS and YS, while the lowest was 0.039 8 between XP and YD. UPGMA analysis was performed based on genetic similarity (Jaccard coefficient). Six populations were clustered into two main groups, one containing populations YS and GT, and the other containing PY, BS, XP and YD. The high genetic variance among populations and the low genetic diversity within population could be attributed to the breeding system of part selfcompatibility and the limited gene flow among populations of H. microcephala.]]></description>
<pubDate>2017/2/9 11:03:17</pubDate>
<category><![CDATA[Special Column：Genetic Diversity]]></category>
<author><![CDATA[SHI YanCai, TANG JianMin, CHAI ShengFeng, ZOU Rong, 
CHEN ZongYou, WEI Xiao*]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>SHI YanCai, TANG JianMin, CHAI ShengFeng, ZOU Rong, 
CHEN ZongYou, WEI Xiao*</atom:name>
</atom:author>
<guid><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170102&flag=1]]></guid><cfi:id>6</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[FMSAP sampling strategy and genetic diversity analysis 
of Castanopsis chinensis in South China Botanical Garden]]></title>
<link><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170103&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Genomic methylation characteristics were influenced by environmental factors. In the study of epigenetics, represented by DNA methylation, the preservation environmental of postharvest samples, especially the samples in the remote place, would have an influence on the later experiment. In this reason, standard sampling and preserve operation were crucial in the field of epigenetic. But the related research was few. Therefore, we studied Castanopsis chinensis in South China Botanical Garden with FMSAP sampling strategy using wilcoxon signed rank tests and unweighted pairgroup method with arithmetic means(UPGMA) methods, in order to find out the best way of sample preservation in five different environments (Liquid nitrogen, -20 ℃, allochroic silicagel, hermetic bag and 75% alcohol). Using the orthogonal experiment method, we optimized the FMSAP system and screened nine pairs of primers (E3H/M2, E5H/M2, E6H/M1, E6H/M5, E8H/M1, E8H/M5, E9H/M2, E11H/M5 and E14H/M1). Additionally, we briefly described different periods of methylation level and genetic diversity. The results showed that hermetic bag preservation was the most appropriate way. And the half and total methylation rate of mature leaves was 27.83% and 51.13%, higher than that of young leaves. The full methylation rate of mature leaves was 23.30%, lower than that of young leaves. The average percentage of polymorphic loci was 39.60%. Shannon information index was 0.207 ± 0.002, which showed a higher level of methylation and genetic diversity.]]></description>
<pubDate>2017/2/9 11:03:17</pubDate>
<category><![CDATA[Special Column：Genetic Diversity]]></category>
<author><![CDATA[LIU Meng1, OUYANG XueJun1,2, CHEN Jie1, QUAN HuiMin1, 
YE WanHui1, LIU Wei1*]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIU Meng1, OUYANG XueJun1,2, CHEN Jie1, QUAN HuiMin1, 
YE WanHui1, LIU Wei1*</atom:name>
</atom:author>
<guid><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170103&flag=1]]></guid><cfi:id>5</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[ISSR analysis on genetic diversity of Liriodendron 
chinense natural populations in Guangxi]]></title>
<link><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170104&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[In order to understand the genetic diversity and distribution model of Liriodendron chinense natural population in Guangxi Zhuang Autonomous Region, the genetic diversity and structure of six natural populations were analyzed by using ten ISSR markers. The results showed that there were abundant genetic diversity in six Liriodendron chinense natural populations. The average (or total) effective number of alleles (Ne), Nei’s gene diversity (H) and Shannon information index (I) of six populations were 1.454 1 (1.633 7), 0.257 6 (0.363 3) and 0.378 6 (0.529 3). A limited gene flow (Nm=1.218 4) resulted in a high genetic differentiation (Gst=0.291 0) among populations. The six populations were divided into two groups depending on cluster analysis (UPGMA method). One group (west group) contains the populations near the west (RSA, HJ and LY) and the other  (east group) included the populations near the east (QZ, ZY and RSL). The east group had a lower genetic diversity and higher differentiation than the west one, which probably implied that populations in east group were strongly disturbed by human activities. Mantel test (R=0.545, P=0.041) displayed a significant isolation by distance model among populations, which also implied that habitat fragmentation due to human activities was an important factor for a highly genetic differentiation among populations. In the light of abundant genetic diversity and highly genetic differentiation on L. chinense populations in Guangxi, not only in situ conservation for the populations with abundant genetic diversity (e.g. RSA, HJ) is critical, but also ex situ conservation by seeds or cuttings collecting for all populations is also essential for further breeding and propagation.]]></description>
<pubDate>2017/2/9 11:03:17</pubDate>
<category><![CDATA[Special Column：Genetic Diversity]]></category>
<author><![CDATA[LI LongMei1, SHI XiaoMeng1,2, JIANG WeiXin1, BAI TianDao1*, 
MENG YiYi1, TAN FeiYan1, HUANG ShouXian1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI LongMei1, SHI XiaoMeng1,2, JIANG WeiXin1, BAI TianDao1*, 
MENG YiYi1, TAN FeiYan1, HUANG ShouXian1</atom:name>
</atom:author>
<guid><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170104&flag=1]]></guid><cfi:id>4</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Efficiency of ISSR markers in assessing genetic 
diversity and relationships in Ardisia 
gigantifolia germplasm]]></title>
<link><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170105&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Ardisia gigantifolia is a medicinal plant that  belongs to genus Ardisia. This plant has been an important traditional Chinese medicine in China for thousands of years. Now, with the development of pharmacological research, A. gigantifolia is not only used as a clinical medicine, but also plays a role in diet and health aspects. The pharmacological validity of its medicinal value has extended in an enlarged market. At present, the wild A. gigantifolia in our country is rare, and the cultivars become the new important source. However, the quality and efficacy of cultivars often suffer from intraspecific variation, unstable quality and other issues. Moreover, the cultivars were also affected by origin, age and environmental conditions. In order to efficiently utilize and conserve this medicinal plant resource, ISSR analysis was employed to reveal genetic diversity of 36 A. gigantifolia germplasm which were collected from different places of Guangxi Zhuang Autonomous Region in China. Data were analyzed by POPGENE32, and a cluster diagram was presented by UPGMA. The results indicated that fourteen ISSR primers generated a total of 136 bands among which 112 (82.35%) were polymorphic bands. Nei’s genetic diversity index (H) was 0.296 5, Shannon diversity index (I) was 0.441 7, and the coefficient of gene differentiation (Gst) was 0.855 8. The genetic similarity coefficient among the populations ranged from 0.667 8 to 0.838 2 in an average of 0.739 1. Based on the clustering analysis, all accessions were clustered into five groups with UPGMA method. Most of the accessions from the same or adjacent regions were clustered into the same group or subgroups. A few accessions, however, were greatly different from the majority of all accessions. The results suggested that ISSR marker was an effective tool for the study of genetic variation in Chinese natural A. gigantifolia germplasm resources. These results can also be used for germplasm characterization and plant breeding.]]></description>
<pubDate>2017/2/9 11:03:17</pubDate>
<category><![CDATA[Special Column：Genetic Diversity]]></category>
<author><![CDATA[MAO ShiZhong1, LI JingJian2, JIANG XiaoHua1, DING Li1, 
ZHAO Bo1*, DENG Tao1, MA HuSheng1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>MAO ShiZhong1, LI JingJian2, JIANG XiaoHua1, DING Li1, 
ZHAO Bo1*, DENG Tao1, MA HuSheng1</atom:name>
</atom:author>
<guid><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170105&flag=1]]></guid><cfi:id>3</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[ISSR analysis on genetic diversity of Keteleeria calcarea]]></title>
<link><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170106&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[ISSR markers were used to investigate the genetic diversity of seven natural populations of Keteleeria calcarea. Twelve ISSR primers produced 125 loci. At the species level, the percentage of polymorphic loci (PPL) was 100.00%, Shannon’s Information index (I) was 0.417 7 and Nei’s gene diversity index (H) was 0.266 6. At the population level, the percentage of polymorphic loci was 71.20%-92.00% and the average value was 80.69%, Shannon’s Information index (I) was 0.327 3-0.388 6 with the average value 0.3548 and Nei’s gene diversity index (H) was 0.213 9-0.247 8 with the average value 0.229 1. K. calcarea in both species and population levels showed higher genetic diversity. Nei’s genetic diversity analysis (Gst=0.143 3) and AMOVA analysis (Φst=17.91%) showed that the genetic variation of K. calcarea mainly occurred within populations, and the genetic differentiation among populations was low. The gene flow (Nm) between populations was high (2.989 0). Mantel analysis showed that the genetic distance of K. calcarea natural populations had no significant correlation with their geographic distance (r=0.456 7, P=0.061 0＞0.05).]]></description>
<pubDate>2017/2/9 11:03:17</pubDate>
<category><![CDATA[Special Column：Genetic Diversity]]></category>
<author><![CDATA[XIE WeiLing, CHAI ShengFeng*, JIANG YunSheng, TANG JianMin, 
CHEN ZongYou, ZOU Rong, WEI Xiao]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XIE WeiLing, CHAI ShengFeng*, JIANG YunSheng, TANG JianMin, 
CHEN ZongYou, ZOU Rong, WEI Xiao</atom:name>
</atom:author>
<guid><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170106&flag=1]]></guid><cfi:id>2</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Genetic diversity analysis on five populations 
of Castanopsis tibetana from Fujian 
Province based on ISSR marker]]></title>
<link><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170107&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Castanopsis tibetana is an evergreen hard wood tree of red Castanopsis, which is one of the excellent commercial tree species. C. tibetana has great development prospect and ecological value in China. In this paper， the genetic diversity of 61 individuals of C. tibetana collected from five plots (Shaowu， Jianyang, Jianou, Zhouning and Pingnan) in Fujian Province of China was analyzed by means of intersimple sequence repeat（ISSR） marker, and the genetic relationship among these individuals also was discussed by cluster analysis method. The results showed that 158 bands were amplified from genomic DNA of 61 C. tibetana individuals by ten intersimple sequence repeat （ISSR）primers, and there were 145 polymorphic bands with percentage of polymorphic band of 91.77%, in which, the percentage of polymorphic band of UBC817, UBC819 and UBC42 reached 100.0%. Percentage of polymorphic band(PPB), effective number of alleles (Ne), Nei’s gene diversity (H) and Shannon’s diversity index (I) were obviously different among five C. tibetana populations，in which all indexes of population in Shaowu were the highest，populations in Zhouning was the lowest. Gene differentiation coefficient and gene flow of five populations was 0.144 0 and 2.973 0，respectively, indicating that 14.40% of overall genetic variation of five populations exists among populations and 85.60% within populations. The gene diversity among species (Ht) and within species (Hs) were 0.395 8 and 0.338 8. It is suggested that the genetic diversity among species of C. tibetana was relatively rich and the interspecies variation degree was higher than intraspecies. Genetic distance among all populations differed obviously, and the genetic distance, between populations of Shaowu and Jianou was the nearest (only 0.081 5) while that between populations of Jianyang and Zhouning was the farthest (0.162 9). Five populations could be divided into three groups by cluster analysis, in which populations of Pingnan and Zhouning were two separate groups，while three populations of Shaowu，Jianou and Jianyang were gathered into one group, which could be divided into two subgroups further, one was Jianyang population and another contained populations of Shaowu and Jianou. It is suggested that populations of C. tibetana tested possessed higher genetic diversity, and there were frequent gene exchange. The genetic diversity of C. tibetana could be accurately revealed by means of ISSR marker analysis．]]></description>
<pubDate>2017/2/9 11:03:17</pubDate>
<category><![CDATA[Special Column：Genetic Diversity]]></category>
<author><![CDATA[TIAN YanLing1,3, LI BoHai2， LI ZhiHui1*, YANG MoHua1, 
ZHANG Bin1, ZHOU XinWei1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>TIAN YanLing1,3, LI BoHai2， LI ZhiHui1*, YANG MoHua1, 
ZHANG Bin1, ZHOU XinWei1</atom:name>
</atom:author>
<guid><![CDATA[http://gxzw.ijournals.cn/gxzwen/ch/reader/view_abstract.aspx?file_no=170107&flag=1]]></guid><cfi:id>1</cfi:id><cfi:read>true</cfi:read></item>
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