Siraitia grosvenorii by High-speed countercurrent chromatography(HSCCC). Crude mogrosides fraction was enriched by macroporous resin method,and then separated by HSCCC. The two-phase solvent system composed of chloroform-methanol-n-butanol-water(5:6:1:4, v/v/v/v)was used for separation. The upper phase was used as the stationary phase, while the lower phase as the mobile phase. The rotation speed was 860 r·min^-1, the flow rate was 2.5 mL·min^-1, and the detection wavelength was 203 nm. 11-O-mogroside Ⅱ, mogroside Ⅱ E, 11-O-mogroside Ⅲ and mogroside Ⅲ, four compounds were prepared in one step,with the purity of 95.5%,98.2%,80.1% and 97.6%, respectively. This method that has the advantages of high sample recovery rate, low loss and avoiding sample inactivation, which successfully realized the rapid and effective separation of mogrosides. Hence, HSCCC can improve separation efficiency and provide theoretical foundation for the separation and purification of moremogrosides, complementing and optimizing the method for separating mogrosides.]]> 2018/5/30 15:44:15 1,2, LU Fenglai², YANG Xuerong², FU Yuxia², LI Dianpeng^2*]]> WU Peijuan^1,2, LU Fenglai², YANG Xuerong², FU Yuxia², LI Dianpeng^2* 8true Nuphar pumilum, which was commonly used by Yao people. The herb medicine can be widely used for relieving cough, reinforcing deficiency, eliminating steam, hidroschesis, removing blood stasis, regulating menstruation and hemostasis. But the method for quality control of N. pumilum is not recorded in different versions of Chinese Pharmacopoeias or local quality standard of herbal medicine. Ten batches of herb medicine were collected in this study. The traits and microscopes of Nuphar pumilum were identified by crude drug identification method, the qualitative determination were performed by TLC, and the contents of gallic acid in these samples were determined by HPLC. The results were as follows:(1)The column was Welch Ultimate XB-C₁₈(4.6 mm × 250 mm, 5 μm)with mobile phase of methanol-0.05% phosphoric acid(5:95)at a flow rate of 1.0 mL·min^-1, detection wavelength was 272 nm, column temperature was room temperature.(2)The identification method for the traits, microscopes and TLC were established.(3)The gallic acid showed a good linear relationship within the range of 0.072 1-0.360 5 μg, whose average recovery(n=6)was 103.66%, with the RSD of 2.03%.(4)Gallic acid contents for the ten batches of samples were 0.82%-1.23%, and the average content was 0.94%. This established quality control method has strong scientificity and can be used for the quality control of N. pumilum.]]> 2018/5/30 15:44:15 1, XUE Yaxin², HUANG Ruisong^1*, SU Qing¹]]> LU Zhenglin¹, XUE Yaxin², HUANG Ruisong^1*, SU Qing¹ 7true 2018/5/30 15:44:16 1, GUO Zhiyong¹, CHEN Xianyi², GU Ronghui¹, LIU Bo¹, LONG Chunlin^1,3*]]> LUO Binsheng¹, GUO Zhiyong¹, CHEN Xianyi², GU Ronghui¹, LIU Bo¹, LONG Chunlin^1,3* 6true Camellia gymnogyna were studied by the methods of ultraviolet spectrophotometry and high performance liquid chromatography(HPLC). The results showed that the component of purine alkaloids, consisted of caffeine(Caf), theobromine(Tb)and theacrine(Tc), had significant differences between the C. sinensis and C. gymnogyna. Tb content was the highest, reached 13.46-39.72 mg·g^-1, Caf content was the lowest, only kept 0.51-2.02 mg·g^-1, and the content of Tc increased with leaves maturing. However, C. sinensis only contained caffeine and theobromine, whose contents respectively varied from 22.22 to 53.13 mg·g^-1, 0.47 to 12.82 mg·g^-1. In the same tea leaf position, catechins component of C. gymnogyna were as follows: EGCG>C>ECG>EGC>EC>GC>CG>GCG, and the total amount of catechins, ester-catechins were significantly lower than C. sinensis, while none-ester catechins content was almost the same, kept 40-50 mg·g^-1. The content of flavonoids demonstrated almost same in different leaf positions while other biochemical components content were as follows: 1st leaf >bud >2nd leaf >3rd leaf >4th leaf, and the contents of two leaf and a bud were between the 1st leaf and 2nd leaf content; while tea polyphenols, flavonoids, theanine and other quality components were lower than those of C. sinensis. This study clarified the changes of the main biochemical quality components of the C. gymnogyna for the first time, especially the composition and proportion of purine alkaloids, which contained the characteristic ingredient-theacrine. The research could be served as important and valuable tea tree resources, and application in alkaloid metabolism mechanism, special tea processing, functional ingredients, low caffeine resources and genetic breeding.]]> 2018/5/30 15:44:16 *]]> TENG Jie, ZENG Zhen, HUANG Yahui^* 5true Marchantia convoluta, we adopted HPLC fingerprint method to study eight batches of medicinal materials. The separation was developed on ZORBAX SB-C18(4.6 mm × 250 mm,5 μm)by gradient elution with acetonitrile-water(both containing 0.1% phosphoric acid)as mobile phase at a flow rate of 1.0 mL·min^-1, at the column temperature of 25 ℃ and with the detection wavelength of 230 nm. The reference fingerprints were established, and six common peaks were found. The similarity of HLPC fingerprints of eight batches of M. convoluta was greater than 0.75. The precision, repeatability and stability of the experiments were in line with the requirements. The data of HPLC fingerprint analysis are stable and reliable. The method is simple and efficient, which can provide reference for the quality control of M. convoluta.]]> 2018/5/30 15:44:16 1, FENG Xu², SU Jian², FU Peng², QIU Jipeng², ZHU Hua^2,3*]]> WANG Yuefeng¹, FENG Xu², SU Jian², FU Peng², QIU Jipeng², ZHU Hua^2,3* 4true -1 and 100 mg·kg^-1 daily by gavage for 60 d to ApoE-deficient mice and experimental hyperlipidemia mice. We measured leves of total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDLC), low density lipoprotein cholesterol(LDLC), alanine aminotransferases(ALT), aspartate aminotransferases(AST), blood urea nitrogen(BUN), creatinine(Cr), glucose(Gru)in serum. The liver of each mouse was extracted, weighted and the li-ver indexes were calculated. Liver TC and TG levels were determined using a semi-automatic biochemical analyzer according to the method described in the tissue TC and TG commercial kit. The results showed that esculeoside A had no effect on levels of ALT, AST, BUN, Cr and Gru, but decreased TC, HDLC, LDLC levels in serum in ApoE-deficient mice, and had no effect on hepatic and renal functions. Esculeoside A could decreased TC and HDLC levels in serum and decreased TC levels in liver in experimental hyperlipidemia mice, and had no effect on hepatic and renal functions. It is indicated that esculeoside A can improve lipid metabolism in ApoE-deficient mice and experimental hyperlipidemia mice, and has no effect on hepatic and renal functions.]]> 2018/5/30 15:44:16 *]]> ZHANG Li, LI Dianpeng, YANG Ziming, LIU Jinlei, HUANG Yonglin, CHEN Yueyuan^* 3true Ilex kudingcha by supercritical CO₂ fluid, the polysaccharides from I. kudingcha were extracted and analyzed by supercritical CO₂ fluid extraction. Phenol-sulfuric acid method was applied for the determination of polysaccharides. Effects of different extraction temperatures(35, 40, 45, 50, 55, 60 ℃), extraction pressures(20, 25, 30, 35, 40, 45, 50 MPa), extraction time(30, 60, 90, 120, 150 min), cosolvents(methanol, 95% methanol, 50% methanol, ethanol, 95% ethanol, 50% ethanol)and cosolvent flow rates(95% ethanol)(2.0, 2.5, 3, 3.5, 4.0, 4.5, 5.0 mL·min^-1)on the polysaccharides extraction rate were investigated and the process conditions for extracting polysaccharides from I. kudingcha by supercritical CO₂ fluid were optimized through orthogonal experiments. Single factor and orthogonal experiments were used to investigate the factors on the yield of polysaccharides. The optimal operation conditions included extraction temperature 50 ℃, extraction pressure 40 MPa, extraction time 150 min, cosolvent flow rate 3.5 mL·min^-1. The concentration of polysaccharides was determined by phenol-sulfuric acid method and the extraction rate was 7.05% under the optimal extraction conditions. The results showed that supercritical CO₂ fluid extraction technology was favorable for extracting polysaccharides from I. kudingcha with the advantages of low extraction temperature, high extraction rate, short extraction period, the environment-friendly and energy-saving.]]> 2018/5/30 15:44:16 1,2*, YIN Huihui^1,2, MENG Fei^1,2, ZHAO Wu^1,2, LIU Wei^1,2, JIANG Yuanming^1,2, QIN Zhenhua^1,2, SUN Jianhua^1,2, CHEN Hailan³]]> WEI Xiaojie^1,2*, YIN Huihui^1,2, MENG Fei^1,2, ZHAO Wu^1,2, LIU Wei^1,2, JIANG Yuanming^1,2, QIN Zhenhua^1,2, SUN Jianhua^1,2, CHEN Hailan³ 2true 50 of ethyl acetate extract fraction and n-butanol extract fraction were higher than VC, Ext. and VC, and the polyphenols contents and antioxidant capacities showed a positive correlation. The extract of walnut leaves could be used as natural antioxidant in food, drugs, cosmetics, health care and other industries. The results of this experiment can provide some theoretical basis for the further development and utilization of walnut resources.]]> 2018/5/30 15:44:16 1,2 , YAN Xiaojie^1*, YANG Jianwen², LI Dianpeng¹]]> WEI Huan^1,2 , YAN Xiaojie^1*, YANG Jianwen², LI Dianpeng¹ 1true