-1), middle(30 mg·kg^-1)and high(60 mg·kg^-1)doses of ethanol extract of Moringa oleifera(EE-MO)leaves was used to interpose the high fat diet-induced non-alcoholic fatty liver disease(NAFLD)mice model. The results were as follows:(1)That oral administration of high dose EE-MO could significantly reduce body weight and wet liver weight of NAFLD mice; EE-MO treatment could decrease the TC, TG, HDL-C and LDL-C contents in serum of NAFLD mice in dose-dependant manner; Apart from decreasing the above parameters, high dose EE-MO treatment significantly reduced FFA content in serum of NAFLD mice.(2)HE and Sudan red Ⅲ staining showed that the fatty lesion and hepatocyte injury of NAFLD mice were improved by EE-MO treatment.(3)It was suggested that EE-MO treatment was capable of improving lipid metabolism of NAFLD mice.(4)High fat diet increased the contents of liver and serum ROS and MDA as well as the activities of SOD, POD and CAT. However, the GSH-Px activity was suppressed by high fat diet.(5)EE-MO treatment could decrease the ROS and MDA contents in liver and serum of NAFLD mice in dose-dependant manner.(6)Oral administration of low dose EE-MO exerted no effect on the activities of SOD, POD, CAT, and GSH-Px of NAFLD mice; However, oral administration of middle and high dose EE-MO significantly reduced the activities of SOD, POD and CAT, but increased the GSH-Px activity; It isuggested that the EE-MO treatment can alleviate the oxidative stress of NAFLD mice by regulating GSH-Px activity.]]> 2019/7/29 11:19:07 1*, LU Yanhua², ZHANG Man², TIAN Fulu²]]> DAI Lifen^1*, LU Yanhua², ZHANG Man², TIAN Fulu² 10true β-hematin formation inhibition assay. As a result, Mussaenda pubescens, Rhodobryum roseum, Glycyrrhiza pallidiflora and other 19 testing samples showed β-hematin formation inhibition activity in different degrees, which came from 17 families and 19 genera. Among the active samples, the water extracts of Woodfordia fruticosa, Ardisia elliptica and Hedychium coronarium, as well as the ethanol extract of Geranium refractum, showed significant inhibition activities with the IC₅₀ values of 796.0, 951.0, 1 033.0, 1 388.9 μg·mL^-1, respectively. HPLC analysis of Woodfordia fruticosa and Ardisia elliptica indicated that their active components should be phenolic constituents.]]> 2019/7/29 11:19:07 1,2, SHEN Yi², XU Wei², LIU Ziqi², PENG Junlin², ZHANG Xiaodong², DONG Xiang^1,2, JIANG Bei^1,2*]]> XIAO Chaojiang^1,2, SHEN Yi², XU Wei², LIU Ziqi², PENG Junlin², ZHANG Xiaodong², DONG Xiang^1,2, JIANG Bei^1,2* 9true -1 and the adsorption temperature was 25 ℃, the removal rate of ethyl paraben was up to 99.7% after SBC adsorption. The reaction increased rapidly at the beginning of 120 min and reached the adsorption equilibrium after 270 min.(3)The isothermal adsorption line for ethyl paraben conforms to the Langmuir mode and the Freundlich mode. The results provided a theoretical reference for the removal of organic pollutants such as ethyl paraben.]]> 2019/7/29 11:19:07 1,2,3,4, CHEN Qi^1,2,3,4, TANG Zhishu^1,2,3,4*, LI Yuan^1,2,3,4, LIU Shijun^1,2,3,4]]> ZHANG Yu^1,2,3,4, CHEN Qi^1,2,3,4, TANG Zhishu^1,2,3,4*, LI Yuan^1,2,3,4, LIU Shijun^1,2,3,4 8true Acanthopanax giraldii belongs to the Araliaceae plant, and most of the Araliaceae plants have a two-way regulation effect in regulating immunity. A. giraldii polysaccharide is a single component extracted from the traditional Chinese medicine A. giraldii. The anti-inflammatory effect of polysaccharides has rarely been reported. This study aimed to explore the protective effect and mechanism of polysaccharide AHP-Ⅱ on LPS-induced hepatocyte injury in rats. This experiment was divided into five groups: blank group, model group(LPS, 40 μg·mL^-1), AHP-Ⅱ low, moderate, high-dose group(25, 50, 100 μg·mL^-1). LPS(40 μg·mL^-1)was used to prepare the rat hepatocyte immune injury model. The cytotoxic effect of AHP-Ⅱ on hepatocytes was first examined by CCK-8 method to eliminate interference, and the secreted level of TNF-α by the cells and the level of ROS were measured by ELISA and flow cytometry respectively to establish the hepatocyte immune injury model. Then the inhibitory effect of different doses of AHP-Ⅱ on inflammatory factors was explored, and the level of JNK2 protein and its phosphorylation were detected by western method to further explore the inhibitory mechanism of AHP-Ⅱ. The results showed that the experimental dose of AHP-Ⅱ had no damage to cells by CCK-8 method, which indicates that the reduction of inflammatory factors secreted by cells treated by AHP-Ⅱ is not due to its cytotoxic effect. AHP-Ⅱ groups(25, 50, 100 μg·mL^-1)decreased TNF-α content durig hepatocyte injury. At the same time, the high-dose AHP-Ⅱ group can reduce the secretion of ROS durig hepatocytes injury, and the high-dose AHP-Ⅱ group has the strongest effect in inhibiting ehe conrent of ROS. After the addition of AHP-Ⅱ, the phosphorylation level of JNK2 protein decreased in a dose-dependent manner, and the AHP-Ⅱ high-dose group had the strongest inhibitory effect. This indicate that the polysaccharide AHP-Ⅱ can play an immunoprotective role by reducing the protein content of P-JNK2 and inhibiting the levels of TNF-α and ROS. The results of this study provide a scientific reference for the hepatoprotective effect of A. giraldii, and promote the further development and utilization of the resources of A. giraldii.]]> 2019/7/29 11:19:07 *, WU Jiang, ZHENG Birong, LI Ruyue, WANG Shuxian, ZHU Yaoqiang]]> LI Qingqing^*, WU Jiang, ZHENG Birong, LI Ruyue, WANG Shuxian, ZHU Yaoqiang 7true Morchella was optimized and its antioxidant activity was studied. Morchella in Beichuan County of Sichuan Province was selected as raw material, adopting alkali extraction method to extract polysaccharide, and then the polysaccharide extraction content was determined by phenol-sulfuric acid method. The extraction process of the polysaccharide was studied by single factor experiment and orthogonal experiment, which controlled extraction temperature(70, 80, 90, 100 ℃), extraction time(2, 4, 6, 8 h), alkali concentration(0.4, 0.6, 0.8, 0.6 mol·L^-1), solid-liquid ratio(1:15, 1:20, 1, 25:30 g·mL^-1). Those antioxidant activities of polysaccharide were also assayed. The results of the optimal extraction conditions were as follows: Extraction temperature was 90 ℃, extraction time 5 h, concentration of alkali concentration 0.7 mol·L^-1 and solid-liquid ratio 1:20 g·mL^-1, the maximum yield of polysaccharide was 5.39%. The polysaccharide of Morchella had a powerful scavenge DPPH free radicals, hydroxyl radicals, superoxide anions, as well as the good reduction capacity, whose IC₅₀ were 0.468, 0.208, 0.022, 0.014 mg·mL^-1, respectively. The polysaccharide of Morchella antioxidant capacity was arranged successively reduction capacity > superoxide anion > scavenging capacity > DPPH free radical scavenging capacity. The optimized extraction process of polysaccharide from Morchella was reasonable, feasible and had strong antioxidant activity.]]> 2019/7/29 11:19:07 1, YANG Ying¹, LU Huimin¹, WANG Jing², LIU Songqing ¹, WANG Fang ^1*]]> ZHOU Yifan¹, YANG Ying¹, LU Huimin¹, WANG Jing², LIU Songqing ¹, WANG Fang ^1* 6true 130N₁₄. The thebaine content was the highest when the nitrogen application were 14 kg· 667 m^-2 and the drip irrigation amount was 130 m³· 667 m^-2.]]> 2019/7/29 11:19:07 1,3, LI Yanrong^1,2*, CHEN Fang^1,3, ZHANG Zhaoping^1,2, WEI Yujie^1,2,3, WANG Junqiang^1,3]]> CHANG Ying^1,3, LI Yanrong^1,2*, CHEN Fang^1,3, ZHANG Zhaoping^1,2, WEI Yujie^1,2,3, WANG Junqiang^1,3 5true Meconopsis horridula is an endangered annual herb belonging to family Papaveraceae. M. horridula is a rare alpine flower, and is a kind of traditional Tibetan medicine. In order to provide a scientific reference for the seed bree-ding and artificial cultivation of M. horridula, the germination characteristics of seeds were studied under the different conditions of disinfector, soaking time, temperature and exogenous plant hormone. The results were as follows:(1)The optimum conditions of seed disinfection method, soaking time, temperature and illumination were 75% ethanol 1 min + 3% H₂O₂ 5 min, 24 h and 20 ℃/10 ℃(illumination 12 h/darkness 12 h), respectively, and the germination rate of disinfected seeds after soaked in sterile water 24 h was 49.67%.(2)Both GA₃ 100-600 mg·L^-1 and NAA 5-30 mg·L^-1 had effects on promotion the germination of seeds, mainly by improving seed germination rate, germination potential and germination index, and shortening germination start time and germination duration time.(3)6-BA 5 mg·L^-1 and 10 mg·L^-1 both could promote the germination of seeds, but the effects were not significant, and when the 6-BA concentration higher than 15 mg·L^-1, the germination of seeds were inhibited.(4)The germination data of M. horridula seeds soaked with GA₃ 500 mg·L^-1 were the best. The germination rate, germination energy and germination index of seeds were 69.67%, 33.00% and 4.51, respectively, and the germination start time and germination duration time of seeds were 10.67 d and 11.67 d, respectively.]]> 2019/7/29 11:19:07 1,2, SONG Kaijie¹, CAI Xitong¹, LAN Xiaozhong^1,2*]]> YUAN Fang^1,2, SONG Kaijie¹, CAI Xitong¹, LAN Xiaozhong^1,2* 4true Pharbitis purpurea, but lack of the systematic and deep studies about the whole plant. It is necessary to investigate the chemical compositions and biological activities of P. purpurea. For better understanding the chemical profile, the major constituents of the whole herb of P. purpurea collected from Dali, Yunnan, were systematically studied. After the whole herb of P. purpurea (4 kg) were extracted with 75% EtOH-H₂O solution, the resulting extract was concentrated and suspended in water, and partitioned successively with EtOAc and n-BuOH. Twelve compounds were isolated from EtOAc-soluble part by various column chromatography methods including silica gel, Sephadex LH-20 and RP18 column chromatography, as well as recrystallization method. Based on the spectral analysis, the compounds were identified as friedelin(1), β-friedelinol(2), β-amyrin(3), α-amyrin(4), 6β-hydroxy-stigmast-4-en-3-one(5), daucosterol(6), β-sitosterol(7), stigmasterol(8), umbelliferone(9), p-hydroxyphenylethanol p-coumarate(10), kaempferol-3-β-D-(6-O-cis-p-coumaroyl)glucopyranoside(11), and glyceroylmonopalmitate(12). Compounds 2-5 and 10-12 were isolated from genus Pharbitis for the first time.]]> 2019/7/29 11:19:07 1,2, XIAO Chaojiang^1,2, LIU Jian², SHEN Yi², DONG Xiang^1,2, JIANG Bei^1,2*]]> LI Yang^1,2, XIAO Chaojiang^1,2, LIU Jian², SHEN Yi², DONG Xiang^1,2, JIANG Bei^1,2* 3true Chrysantha Chang in Camellia including C. nitidissima, C. impressinervis and C. chuangtsoensis were qualitatively and quantitatively analyzed by ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry. The results showed that fifteen flavonoids were detected in flower from three species. The flavonoids including pelargonium-3-O-glucoside, luteolin, luteolin-7-O-rutinoside, quercetin-3,7-O-diglucoside, narirutin, eriodictyol and genistin were identified for the first time in section Chrysantha Chang, and quercetin-3-O-glucoside, quercetin-7-O-glucoside, quercetin-3-O-rutinoside and kaempferol-3-O-glucoside were identified in flowers of C. impressinervis and C. chuangtsoensis for the first time. Catechin, epicatechin, quercetin-3-O-glucoside, quercetin-7-O-glucoside, quercetin-3-O-rutinoside and kaempferol-3-O-glucoside were the main flavonoid components of three species. Pelargonium-3-O-glucoside was the endemic components of C. nitidissima, and quercetin-3,7-O-diglucoside was the endemic components of C. chuangtsoensis. Luteolin-7-O-rutinoside was mainly in C. nitidissima and C. chuangtsoensis, and luteolin was mainly in C. impressinervis and C. chuangtsoensis. The main flavonoids types in three species were catechins, quercetins, luteolins and kaempferols. The contents of quercetins, luteolins and total flavonoids in C. chuangtsoensis were far higher than that in C. nitidissima and C. impressinervis. Catechins in C. impressinervis and C. chuangtsoensis were higher than that in C. nitidissima, and kaempferols in C. nitidissima and C. chuangtsoensis were higher than that in C. impressinervis.]]> 2019/7/29 11:19:07 1*, WANG Jiatong^1,2, SUN Zhenyuan², WANG Jie¹, YIN Hengfu¹, FAN Zhengqi¹, LI Jiyuan¹]]> LI Xinlei^1*, WANG Jiatong^1,2, SUN Zhenyuan², WANG Jie¹, YIN Hengfu¹, FAN Zhengqi¹, LI Jiyuan¹ 2true Tetrastigma hemsleyanum is a kind of medicinal herbs, in which total flavonoids have anti-cancer effects. In recent years, with the increasing medical demand, it is imperative to explore the economic and biomimetic cultivation under the trees. The purpose of this study was to investigate total flavonoids contents, physiological and biochemical characteristics of T. hemsleyanum in Zhejiang Province responsed to different light intensities. Biennial cuttings were used as experimental materials and five light gradients(full light, shade 30%, 50%, 70% and 90%)were set up. After six months' treatment, we determined the growth parameters(growth, biomass), biochemical parameters [soluble protein(SP), free proline(FP), superoxide dismutase(SOD)] and contents of total flavonoids in different parts(leaves and rhizomes). The results showed that total length of the stems, maximum length and width of leaves, weight of single rhizomes increased first and then decreased with the decrease of light intensity; specific leaf weights decreased overall. Contents of SP, FP and SOD in leaves were the highest with full light conditions, the lowest with 70% shading(lower than total light was 33.36%, 17.22%, 46.88%). It showed that excessive high light and low light both affected photosynthesis and accumulation of active ingredients. Total flavonoid contents in leaves were higher than those in rhizomes, and the highest values of leaves(6.16 mg·g^-1)and rhizomes(2.21 mg·g^-1)were treated with 70% shading. In general, T. hemsleyanum grew best and suffered lowest stress in 70% shading treatments. The study indicates that light intensity is an important factor affecting the growth and accumulation of active constituents in T. hemsleyanum of Zhejiang Province, and high light intensity inhibits the growth of T. hemsleyanum to a certain extent, and appropriate shading promotes the growth. The total flavonoid content in the leaves responded differently to light intensity, which provided a scientific reference for scientific cultivation and rational development of T. hemsleyanum in the future.]]> 2019/7/29 11:19:07 1,2, LIU Shouzan³, BAI Yan^1,2*, XU Linyu^1,2, DING Heng^1,2, WU Xueqian^1,2, XU Haishun^1,2, ZHENG Bingsong^1,2]]> HU Xiaotian^1,2, LIU Shouzan³, BAI Yan^1,2*, XU Linyu^1,2, DING Heng^1,2, WU Xueqian^1,2, XU Haishun^1,2, ZHENG Bingsong^1,2 1true