Castanea henryi has very high edible and medicinal values, which is an important woody grain and a characteristic fruit tree resource in South China. In order to find out the best treatment strategy to improve the germination rate of C. henryi seeds, and provide scientific references for its exploration and utilization, effects of temperature, light, matrix, matrix moisture and shell-breaking treatment on seed germination of C. henryi were investigated. The results were as follows:(1)The suitable temperature for germination was 25 ℃, the final germination percentage was 92.67%, and the germination percentage under other temperature conditions decreased, but had no significant differences with that under 25 ℃.(2)The germination percentage and germination energy under the dark regime was significantly higher than that under alternating light regime, but the root length under alternating light regime was significantly longer than that under the dark regime.(3)Peat soil had the best effect on seed germination and seedling growth, the germination percentage and germination energy in peat soil were higher than that in sand, and the root length in peat soil was significantly longer than that in sand.(4)The matrix moistures had no significant effect on seed germination percentage and germination energy.(5)The mechanical barrier of seed shell was one of the limiting factors of seed germination. After shell-breaking treatment, the seed germination energy and root length were significantly different from those without shell-breaking. Shell-breaking treatment could accelerate seed germination in actual production.]]> 2020/3/13 15:53:11 1,2, CHEN Zongyou^1,2*, TANG Hui^1,2, WANG Manlian², WEI Xiao²]]> LI Jitao^1,2, CHEN Zongyou^1,2*, TANG Hui^1,2, WANG Manlian², WEI Xiao² 34true Arundo donax, through potting experiment in outdoor, by selecting Acorus tatarinowii, Iris tectorum, Canna indica and Arundo donax as experimental materials. The mixed patterns were formed by Acorus tatarinowii + Arundo donax, Iris tectorum + Arundo donax, Canna indica + Arundo donax, and Arundo donax was taken as control. The results were as follows:(1)Compared with the single pattern, mixed planting patterns with three aquatic plants improved the root-shoot ratio of A. donax under the different concentrations of lead pollution. With the increase of lead pollution concentration, the root-shoot ratio showed an upward and then downward trend.(2)The lead content in the body and the soil of A. donax showed obvious concentration accumulation effect in the single and mixed planting patterns. The lead content in plants and soil in mixed plan-ting patterns was significantly lower than that in the single planting pattern, and the differences were significant between single and mixed planting patterns(P<0.05).(3)Under the treatment of high concentration lead pollution, the translocation factor of A. donax was greater than 1 in the single and mixed planting patterns. However, the bioconcentration factor was greater than 1 in the low and high concentrations of lead pollution treatments. In the Canna indica + Arundo donax pattern, the lead bioconcentration factor of Arundo donax was the largest in both above-ground and below-ground parts. Therefore, we believed that Canna indica + Arundo donax pattern significantly increased the biomass and root-shoot ratio of Arundo donax, and had the best purification ability to lead contaminated soil.]]> 2020/3/13 15:53:11 *]]> ZHUANG Jingjing, WANG Xiaobing, GUO Hui^* 33true 2020/3/13 15:53:11 1, 2, XU Peng¹ , YU Diqiu^1*]]> TANG Ruyu^{1, 2}, XU Peng¹ , YU Diqiu^1* 32true Firmiana pulcherrima and F. major. Fifty-two genes selected from chloroplast genome(Cp genome)of these two species were analyzed using CodonW, CUSP and SPSS software. The results showed that:(1)GC content in Firmiana pulcherrima was 38.12%, and that in F. major was 38.05%, suggesting that A/T base was rich in chloroplast genome.(2)The effective number of codons in F. pulcherrima ranged from 36.91 to 56.46, and that in F. major ranged from 36.55 to 58.04, meaning the codon bias was weak in F. pulcherrima and F. major.(3)Both F. pulcherrima and F. major had 29 codons with relative synonymous codon usage(RSCU)greater than 1 and 28 codons ending with A and U base.(4)Neutrality plot showed that there was not significant correlation between GC₃ and GC₁₂, and the regression slope was 0.195 in F. pulcherrima, and 0.304 in F. major, suggesting that codon bias was mainly affected by natural selection.(5)ENC-plot analysis showed that most of genes fell below and followed the standard curve, and ENC ratio of most genes ranged from -0.04 to 0.10, suggesting that the mutation also affected the formation of codon bias in F. pulcherrima and F. major. In addition, 17 codons were identified as optimal codons in F. pulcherrima, and 18 codons in F. major. We suggest that the codon usage bias of F. pulcherrima and F. major are formed under effect of mutation and selection. Although the codon usage pattern of F. pulcherrima and F. major are very similar, there are some differences, which may be related to the evolutionary me-chanism of adaptive environment.]]> 2020/3/13 15:53:11 *]]> LU Qifeng, LUO Wenhua, HUANG Zhihuan^* 31true Fusarium wilt is a devastating soil-borne disease which had limited the production of gourd vegetables. Grafting cultivation is an effective method to control F. wilt. In order to obtain rootstock-used gourd germplasm with strong disease resistance and to select hybrid combinations, the correlation between seedling growth index and disease resistance was discovered, and the heterosis of disease resistance was analyzed. The results were as follows:(1)Rootstock-used gourd germplasm H041 showed high resistance(HR)to watermelon F. wilt and resistance(R)to clavated calabash F. wilt, respectively. And two rootstock-used gourd germplasm(H01 and H05)and three hybrid combinations(H01×H041, H041×H05 and H05×H041)showed resistance(R)to these two kinds of F. wilt.(2)The di-sease index of rootstock-used gourd seedlings was significantly negatively correlated with hypocotyl coarseness after treating with watermelon F. wilt. And the root length and root surface area of the seedlings had significantly negative correlation with disease index after treating with clavated calabash F. wilt. This indicates that the resistance of the disease is correlated with root growth. The resistance of the disease can be evaluated rapidly according to root growth.(3)Furthermore, the disease-resistance hybrid heterosis of rootstock-used gourd germplasm showed that, hybrid combination H05×H041 showed negative mid-parent heterosis to watermelon F. wilt. H01×H041, H02×H041, H041×H01 and H05×H041 showed negative heterosis for resistance to clavated calabash F. wilt. In conclusion, rootstock-used gourd germplasm H01, H041 and H05 showed resistance against two kinds of F. wilt, and there was resistance heterosis on the hybrid combinations of these germplasms. Hence, H01, H041 and H05 could be used as rootstock or elite parents for resistance breeding against two kinds of F. wilt, and H041 could be used as the backbone parent.]]> 2020/3/13 15:53:11 *]]> LIAO Jianjie, ZHANG Yingqing, XU Boya, ZHENG Xuyang, YANG Yanjuan, ZHONG Chuan, YU Wenjin^* 30true 2020/3/13 15:53:11 1 , NIU Suyan ¹ , ZHOU Yiran ² , CUI Bo ¹ , LIANG Fang ¹ , YUAN Xiuyun ¹ , MA Jie ^{1 *}]]> JIANG Suhua ¹ , NIU Suyan ¹ , ZHOU Yiran ² , CUI Bo ¹ , LIANG Fang ¹ , YUAN Xiuyun ¹ , MA Jie ^{1 *} 29true Gentiana rigescens, while 8-hydroxygeraniol oxidoreductase gene Gr8HGO is a structural gene involved in gentiopicroside biosynthesis. In order to study the function of Gr8HGO gene, the Gr8HGO gene in G. rigescens was cloned and its expression analysis was conducted in this study. The results were as follows:(1)Five Gr8HGO genes were cloned and their GenBANK accession numbers were KP722029.1(Gr8HGO-1), KP722030.1(Gr8HGO-2), KP722031.1(Gr8HGO-3), KP722032.1(Gr8HGO-4)and KP723852.1(Gr8HGO-5), separately.(2)The length of Gr8HGO-1 gene was 1 062 bp encoding 353 amino acids, while the other four genes were 1 131 bp encoding 376 amino acids; The results from physicochemical analysis showed that the relative molecular weight of these five Gr8HGO proteins were approximately 40 kD, and their theoretical pI ranged from 5.47 to 5.95, which were all hydrophobic stable proteins.(3)Signal sequence analysis showed that five proteins did not contain signal peptides, transmembrane helixes and chloroplast transit peptides; Subcellular localization analysis indicated that these five proteins might be localized in cytoplasm; Domain prediction results showed that beside the Gr8HGO-1 contained only alcohol dehydrogenase N-terminal(IPR013154)and C-terminal(IPR013149)conserved domains, the other four also contained polyketide synthase, enoylreductase domain(IPR020843).(4)Phylogenic analysis showed that these five Gr8HGO proteins had the closest relationship with Cr8HGO in Catharanthus roseus.(5)The results of qPCR suggested that Gr8HGO gene was mainly expressed in leaves, but very low in roots and stems. This study will lay a foundation for further analysis of the biosynthesis pathway of gentiopicroside.]]> 2020/3/13 15:53:11 *]]> WANG Weiyan, LI Caixia, LI Qiu, YAN Qiuzi, ZHANG Xiaodong^* 28true in vitro using the DPPH free radical, ABTS free radical and iron ion reduction antioxidant(FRAP)assays, and we also determined the phenolic compositions of the seed extracts by the high performance liquid chromatography(HPLC). The results were as follows:(1)The contents of total phenolics and total flavonoids were significantly higher in the seeds of Chuanqiao 1 than those of Jiujiang Kuqiao, with the former standing at 27.38 mg GAE·g^-1 DW and 31.46 mg RE·g^-1 DW and the latter at 12.71 mg GAE·g^-1 DW and 14.68 mg RE·g^-1 DW; in the seeds of the two varieties, the contents of the free phenolics and flavonoids, which made up more than 79% of the total phenolics and flavonoids, were significantly higher than those of bound phenolics and flavonoids; the contents of bound phenolics and flavonoids were higher in the seeds of Jiujiang Kuqiao than those of Chuanqiao 1.(2)Rutin, quercetin, epicatechin, kaempferol, kaempferol-3-rutinoside and quercetin-3-O-rutinoside-3'-O-glucopyranoside dominated among the phenolic compounds in the seeds of the black tartary buckwheat varieties, with rutin and quercetin dominant phenolics among the free phenolic compounds and epicatechin and rutin dominant among the bound ones.(3)All the seed extracts of the tartary buckwheat varieties presented antioxidant activities and the antioxidant activities of the free seed extracts of Chuanqiao 1, determining as being 30.14, 11.03 and 18.84 mg TE·g^-1 DW using DPPH, ABTS and FRAP, respectively, were higher than those of Jiujiang Kuqiao. The antioxidant activities of the bound extracts of Chuanqiao 1 were lower than those of Jiujiang Kuqiao, but the total antioxidant acti-vity of the former variety was significantly higher than that of the latter one. Therefore, planted in low-elevation regions of the Jianghan Plain, Chuanqiao 1 had higher contents of seed phenolic compounds, as being required in subsequent food processing and presenting a bright market prospect.]]> 2020/3/13 15:53:11 1, HOU Zehao¹, WEI Shudong², FANG Zhengwu^1*]]> SUN Kunkun¹, HOU Zehao¹, WEI Shudong², FANG Zhengwu^1* 27true 2020/3/13 15:53:11 1, WANG Yan^1,2, LIANG Chenggang^1,2*, LIAO Kai¹, WEI Chunyu¹, FU Quanlan¹, ZHANG Wei¹, GUAN Zhixiu¹]]> YU Wujuan¹, WANG Yan^1,2, LIANG Chenggang^1,2*, LIAO Kai¹, WEI Chunyu¹, FU Quanlan¹, ZHANG Wei¹, GUAN Zhixiu¹ 26true Quercus wutaishanica by utilizing pot planting method. The results were as follows:(1)Germination percentage(GP), germination rate(GR), germination value(GV), and germination index(GI)of large Q. wutaisha-nica seeds were higher than those of small seeds at all sowing depths. Significant differences between large and small coated seeds in GP, GR and GI were observed at 0 cm sowing depth, while significant differences between large and small uncoated seeds in all germination parameters were detected at 6, 10 cm sowing depths. Peeling could facilitate the germination of large seeds but inhibit the germination of small ones. All germination parameters of different sized seeds maximized at 0 cm sowing depth and minimized at 10 cm sowing depth.(2)Regardless of whether coated or uncoated, leaf number(LN), leaf area per plant(LAPP), total dry mass(TDM), and root-shoot ratio(RSR)of seedlings germinated from large seeds were all higher than those of seedlings from small seeds at all four sowing depths. The shoot height(SH), basal stem diameter(BSD), LN, LAPP, and TDM of seedlings established from uncoated seeds were all lower, to various degrees, than those of seedlings from coated seeds at all sowing depths, while the RSR of the former were higher than those of the latter at 0, 3, 6 cm sowing depths. The SH, LN, LAPP, TDM, and RSR of seedlings all exhibited decreasing trends with the increasing of sowing depth. However, the BSD of seedlings increased with the sowing depth increasing.]]> 2020/3/13 15:53:11 *, LUO Yonghong, ZHU Yating]]> ZHANG Jinfeng, CHENG Jiming, YAN Xingfu^*, LUO Yonghong, ZHU Yating 25true Fusarium oxysporum f. sp. cubense is the pathogen of banana fusarium wilt which is a soil habitual fungus. Understanding the diversity of fungi and the proportion of Fusarium in the soil of banana root zone has important guiding significance for reducing the pathogens in the soil and preventing the occurrence of banana fusarium wilt. The root zone soil samples from banana healthy and fusarium wilt plants with different perennial years were collected and the fungal populations in the soil samples were determined by high throughput sequencing technique. The results were as follows:(1)Among banana plants with the same perennial root age, the number of reads and OTUs in the samples from healthy plants was higher than that from fusarium wilt plants, indicating that the diversity of fungi in the root zone soil of healthy plants was richer than that from fusarium wilt plants.(2)Except for phylum Basidiomycota was dominant in annual banana fusarium wilt plant, Ascomycota was the dominant phylum in other soil samples. The highest relative abundance of family Nectriaceae was in the root zone soil of triennial healthy plant(26.02%), followed by that of five-year-old fusarium wilt plant(15.56%).(3)Among genus family Nectriaceae, the highest relative abundance of Fusarium was 2.54% in the sample from triennial healthy plant, and that from other samples ranged from 0.1% to 0.65%. Among genus Fusarium, the relative abundance of Fusarium solani(0-1.59%)was higher than that of F. oxysporum, whose relative abundance only ranged from 0 to 0.08%. It was concluded that the diversity of fungi in the root zone soil of banana healthy plants was higher than that of fusarium wilt plants. The population of Fusarium or F. oxysporum, which were the pathogen of banana fusarium wilt, was not the dominant genus or species in root zone soil from both healthy and fusarium wilt plants.]]> 2020/2/21 14:10:07 *]]> LIAO Yongmei, HUANG Yuantengji, ZOU Chengwu, HUANG Qi, WANG Zhongwen^* 24true Cyclocarya paliurus is a unique fast-growing tree in China and a mono species of Cyclocarya genus in Juglandaceae family. It has important medical value and is honored as “the third tree in the medical field”. But its seedling breeding is difficult and inefficient. Therefore, tissue culture and rapid propagation of Cyclocarya paliurus were studied by using the young stem segments as explants. The results were as follows: The optimal sampling time of explants was from April to June. The best explants for tissue culture were the slight lignification stem segments. And the best explants surface disinfection method was to soak stem segments in 0.1% HgCl₂ for about 5-7 min. The success rate of disinfection was 54.1% and the survival rate of aseptic explants was 88.7%. The medium of MS+6-BA 2.0 mg 瘙 簚 L^-1+IBA 0.2 mg 瘙 簚 L^-1+sugar 30.0 g 瘙 簚 L^-1 was suitable for primary bud induction culture, and the bud induction rate was 80.5%. After 21 d cultivation, the average height of primary buds was 3.0 cm. The best subculture medium for multiplication was MS+6-BA 0.5 mg 瘙 簚 L^-1+IBA 0.05 mg 瘙 簚 L^-1+TIBA 0.02 mg 瘙 簚 L^-1+sugar 30.0 g 瘙 簚 L^-1. After 35 d of cultivation, the proliferation coefficient was 7.0/35 d, and the shoots were strong and free of vitrification with an average height of 4.5 cm. The medium for strong shoots before rooting was MS+6-BA 0.5 mg 瘙 簚 L^-1+IBA 0.05 mg 瘙 簚 L^-1+sugar 30.0 g 瘙 簚 L^-1. Cultivated in this medium for 35 d, the shoots grew tall and strong, with an average height of 6.0 cm. The rooting medium was 1/2 WPM+IBA 1.5 mg 瘙 簚 L^-1+5-NGS 4.5 mg 瘙 簚 L^-1+sugar 20.0 g 瘙 簚 L^-1. Cultivated 40 d in this medium, and the highest rooting rate was 83.3%. The suitable transplanting matrix for rooting seedlings was slimy soil, and the best transplanting seasons were the periods from March to May and from October to November. After transplanting, the rooting seedlings were cultured for 40 d in a greenhouse with a shade of 70%, and the survival rates were 54.3%-65.6%. This work would lay a foundation for large-scale breeding of its superior clones.]]> 2020/2/21 14:10:07 *]]> HUANG Ningzhen, SU Jiang, XIAN Kanghua, FU Chuanming, GONG Qingfang, HUANG Huijin, HE Jinxiang^* 23true Rosa roxburghii, R. roxburghii GLABROUS 2(RrGL2), a prickle-development related AtGL2 homology gene, was isolated from ‘Guinong 5' and relative biological information and expression were analyzed in this paper. The cytological development of fruit thorn of Rosa roxburghii was observed by paraffin section. Leaves of Rosa roxburghii was used to synthesize cDNA based on the manufacturer's instructions of RACE. Subsequently RrGL2 was made relative informatics analysis and the gene expression level was eva-luated. The results were as follows:(1)The base cells continuously divided at the early stage of flower bud, then outward developed. The middle cells continued to become thinner and longer to form a “needle” structure. In the early stage of flower bud formation, the cells in the base of the thorn structure first divided continuously and continued to develop outwards. The cells in the middle became thinner and longer, forming a “needle” structure. The lignification gradually was observed at the top cells to make the prickles hard.(2)The full lengths of RrGL2 was 2 292 bp by RACE, encoding 763 amino acids.(3)The RrGL2 had a structure of Homeodomain and StAR phosphatidylcholine transfer protein, which is likely to regulate the development of Rosa roxburghii prickles. Then, a search for homologous species in the NCBI databases revealed a high similarity of amino acid homology encoded by the RrGL2 with other Rosa species, and phylogenic analysis revealed a close relationship of structure domains between Rosa roxburghii and Fragaria vesca.(4)Finally, real-time PCR analysis showed that the relative expression value of RrGL2 in fruit prickles after seven weeks after flowering was the highest, almost respectively 7.87 times and 2.10 times than that during three weeks and during five weeks after flowering. RrGL2, a prickles-forming gene acted to regulate the morphology and development of prickles. Therefore, the function of RrGL2 is closely related to thorn formation. These results could provide theoretical basis for thorn formation and development.]]> 2020/2/21 14:10:07 1,2, CHEN Tingting^1,2, ZHANG Qinqin^1,2, MO Jiajia^1,2, GONG Panqin^1,2, YAN Huiqing^1*]]> HUANG Xiaolong^1,2, CHEN Tingting^1,2, ZHANG Qinqin^1,2, MO Jiajia^1,2, GONG Panqin^1,2, YAN Huiqing^1* 22true LcUFGT which is a structural gene of anthocyanin synthesis pathway was cloned and analyzed by bioinformatic methods. The corelation between LcUFGT and the character of late-maturing in ‘GLL-1' and the expression of LcUFGT during the development of litchi fruit were analyzed by qRT-PCR. The results were as follows:(1)ORF of LcUFGT gene was 1 359 bp, which encoded 453 amino acids with the molecular weight of 50.16 kD.(2)LcUFGT gene was conservative in ‘GLL-1' and other litchi cultivars, and a PSPG box existed in the C-terminal.(3)With the development of litchi fruit, the color of pericarp began to turn from green to red; the expression of LcUFGT was increased and then decreased in both ‘GLL-1' and ‘Heli'; the expression of LcUFGT was obviously increased at 56 d and 67 d after flowering of ‘Heli' and ‘GLL-1', respectively; the expression of LcUFGT had a delayed increase in ‘GLL-1' than that of ‘Heli', which was consistent with the late-maturing of fruits. It is supposed that LcUFGT plays an important role in color changes of pericarp, and it is one of the key genes to regulate coloration of pericarp. At the same time, the delay-expression of LcUFGT maybe the main reason of late-maturing of ‘GLL-1'.]]> 2020/2/21 0:00:00 1, PENG Hongxiang¹, PAN Jiechun², LI Hongli¹, QIN Xianquan¹, ZHU Jianhua¹, LI Dongbo¹, XU Ning¹, HOU Yanjie¹, QIU Hongye¹, LI Ping³, WANG Jinying², DING Feng^1*]]> ZHANG Shuwei¹, PENG Hongxiang¹, PAN Jiechun², LI Hongli¹, QIN Xianquan¹, ZHU Jianhua¹, LI Dongbo¹, XU Ning¹, HOU Yanjie¹, QIU Hongye¹, LI Ping³, WANG Jinying², DING Feng^1* 21true Dracaena cambodiana)are the main plant resources of dragon's blood in China, and the main chemical constituents of the dragon's blood are flavonoids. The recent studies about dragon's blood mainly focus on the chemical constituents and pharmacological activities, while the molecular mechanisms of dragon's blood formation remain unknown. WD40 transcription factor plays an important role in flavonoid accumulation. In this study, a WD40 gene named DcWD40-1 was cloned in Dracaena cambodiana based on transcriptome data and RT-PCR techno-logy. The full-length of cDNA of DcWD40-1 was 1 550 bp, containing 1 353 bp opening reading frame(ORF), and encoding 450 amino acids with the calculated molecular weight of 50.77 kD and calculated pI 5.71. Bioinformatics analysis showed that DcWD40-1 belonged to a member of WD40 superfamily, had five conserved WD40 domains, and shared high identities to WD40 proteins with other plants. A 1 503 bp-length promoter region of DcWD40-1 was isolated by Genome Walking method, which had structural characteristics of typical eukaryotic promoters. The promoter region of DcWD40-1 contained lots of hormone responsible elements, such as abscisic acid-responsive element, auxin-responsive element, salicylic acid-responsive element and jasmonic acid-responsive element; also had many cis acting elements related stress such as light, cold, hot and anaerobic inducer. Expression analysis showed that DcWD40-1 was induced by dragon's blood inducers, positively related to flavonoids accumulation and formation of dragon's blood. In addition, DcWD40-1 can also respond positively to jasmonic acid, cytokinin, brassinosteroid and UV-B treatment. These results will lay the foundation for further study of the potential functions and mechanisms of DcWD40-1 in flavonoid biosynthesis in Dracaena cambodiana.]]> 2020/2/21 14:10:07 1, XIA Dongnan2, WANG Ying1, GUO Dong1, LI Huiliang1, MEI Wenli1, PENG Shiqing1*]]> ZHU Jiahong1, XIA Dongnan2, WANG Ying1, GUO Dong1, LI Huiliang1, MEI Wenli1, PENG Shiqing1* 20true 2020/12/14 9:34:21 WANG Xianzhi1，2， NIU Lixin1， XIE Songlin3*， LUO Jianrang1 19true Marchantia polymorpha under cadmium stress were studied with incubation method in lab. Male and female gametophytes were collected from wild and regularly treated with 100 mg·L^-1 of CdCl₂ solution, then the cadmium treated samples were collected regularly for determination of physiological indexes. Meanwhile, the comprehensive evaluation value of male and female gametophytes, which calculated by subordinate function method, was used to evaluate the differences in cadmium tolerance. The results were as follows: Each physiological index had a similar trend of change, the activity of SOD, the contents of MDA and GSH increased significantly with stress day, the POD activity increased markedly after an initial decrease, the contents of soluble protein and proline enhanced significantly under short-term stress and then decreased gradually, and the contents of chlorophyll a(chla)and chlorophyll b(chlb)reduced dramatically with stress days; The value of chlorophyll a/chlorophyll b(chla/chlb)of female gametophyte had a significant increase, while it increased significantly only for 8 d in male gametophyte; The comprehensive evaluation value showed the cadmium tolerance ability of male M. polymopha gametophyte was better than female M. polymopha gametophyte. The results provide a reference for the further study of gender differences of dioecious plants under the different stress.]]> 2020/12/14 9:34:21 *]]> YANG Ruhua, JIN Tang, DAI Yuxuan, WANG Li^* 18true in vitro embryos and the growth of regenerated plants were examined in immature in vitro embryos from Paris polyphylla var. chinensis seeds. The main purpose was to explore the basic system of in vitro embryos culture and growth of regenerated plants in P. polyphylla var. chinensis. In order to study the effects of plant-growth regulators on the growth and development of immature in vitro embryos and the growth of regenerated plants, supplemented with plant-growth regulators with different kinds and concentrations to MS mediums. In order to study the effect of light on the growth and development of immature in vitro embryos of P. polyphylla var. chinensis, the immature in vitro embryos were cultured in light and dark conditions. The results showed that dark conditions were more conducive to the growth and development of immature in vitro embryos in P. polyphylla var. chinensis than light conditions. When cultured for 60 d, the growth rate and germination rate of immature in vitro embryos of P. polyphylla var. chinensis under dark culture conditions were 45.25%, 19.17% higher than those under light culture conditions, respectively. When the concentration of GA₃ was the same, the germination time of immature in vitro embryos was prolonged as the concentration of IAA increased. Different concentrations of GA₃ promoted the germination of immature in vitro embryos. The promoting effect was 5 mg·L^-1 GA₃>1 mg·L^-1 GA₃> 10 mg·L^-1 GA₃. Under dark conditions, the best formula for immature in vitro embryos of P. polyphylla var. chinensis germination was 1/2 MS + 30 g·L^-1 sucrose + 7 g·L^-1 agar + 0.5 g·L^-1 activated carbon + 5 mg·L^-1 GA₃ + 1 mg·L^-1 IAA. This induced in vitro embryo P. polyphylla var. chinensis germination in about two months. The germination rate reached 50% after about 80 d. In the mature embryos emergence experiment, a high concentration of plant-growth regulators inhibited the growth and development of mature embryos in P. polyphylla var. chinensis. A high concentration of GA₃ in particular led to the death of mature embryos. The best formula for seedlings of mature embryos was MS + 30 g·L^-1 sucrose + 6.2 mg·L^-1 boric acid + 7 g·L^-1 agar + 0.7 g·L^-1 activated carbon + 0.5 mg·L^-1 2,4-D + 1.5 mg·L^-1 IAA + 1.5 mg·L^-1 ZT + 5 mg·L^-1 GA₃. Mature embryos could produce seedlings within only 43 d. True leaves were formed after around 75 days.]]> 2020/12/14 9:34:21 1,2, ZHOU Hanmei¹, HE Bing^1*, LI Wei¹]]> CHEN Yao^1,2, ZHOU Hanmei¹, HE Bing^1*, LI Wei¹ 17true Metasequoia glyptostroboides is very difficult in nature, and seedlings and saplings are rarely seen under the forest. Low spring temperature in original habitat of M. glyptostroboides is an important factor causing the difficulty of natural regeneration. To explore whether the reason for the low seed germination rate is related to the spring low temperature of the original species of M. glyptostroboides. We used constant temperature incubator to set three kinds of constant temperature treatment(15, 20, 25 ℃)and one kind of temperature change treatment(12 ℃/12 h and 24 ℃/12 h, alternating every 24 h); set five different concentrations of polyethylene alcohol(PEG-6000, 0, 0.05, 0.10, 0.15, 0.20 g·mL^-1)mimics drought stress. The results were as follows:(1)The temperature set by the experiment had no significant effect on the germination of M. glyptostroboides seed(P>0.05). The average germination rate and germination potential of M. glyptostroboides seeds under variable temperature conditions were the highest, which were 46.75% and 21.25%, respectively;(2)Different concentrations of PEG-6000 solution had significant effects on seed germination(P< 0.05), at the temperatures of 15 ℃ or 25 ℃, low concentration of PEG-6000 solution(0-0.10 g·mL^-1)promoted the germination potential and germination index of M. glyptostroboides seeds, high concentration(>0.10 g·mL^-1)PEG-6000 solution inhibited the germination of M. glyptostroboides seed, and the concentration increase inhibition also increased, and until the concentration increased to 0.20 g·mL^-1, the M. glyptostroboides seeds no longer germinated. The results indicate that the low temperature in the spring is not a factor that causes the natural regeneration of the original population of M. glyptostroboides, and appropriate drought stress is beneficial to enhance the germination potential and germination index of M. glyptostroboides seeds, and to promote the emergence of seedlings.]]> 2020/12/14 9:34:21 2, ZHU Jiang ^1,2*, AI Xunru^1,2, YAO Lan^1,2, HUANG Xiao², WANG Jin², ZHU Qiang², CHEN Shaolin³]]> WU Manling², ZHU Jiang ^1,2*, AI Xunru^1,2, YAO Lan^1,2, HUANG Xiao², WANG Jin², ZHU Qiang², CHEN Shaolin³ 16true 2021/8/31 16:11:24 1,2,3, HAN Li^1,2,3, ZHAOYiting^1,2,3, ZHOU Xuan^1,2,3, DU Yunlong^1,2,3*]]> WU Lixia^1,2,3, HAN Li^1,2,3, ZHAOYiting^1,2,3, ZHOU Xuan^1,2,3, DU Yunlong^1,2,3* 15true Neolamarckia cadamba is one of the most important fast-growing trees for wood production in South China, and its secondary growth depends on the development process of vascular tissue cells including formative cells. In order to obtain the vascular tissue accurately and high quality RNA of N. cadamba, we presented an optimal method to provide high-quality paraffin section for vascular tissues, in which carnoy/acetone was used as fixative(acetone concentration was 10%-50%)and 100% n-Butanol was used as the dehydrating and infiltration agent, followed by gradient infiltration paraffin, embedding, sectioning and dewaxing. Meanwhile, LMD parameters were set with magnification between 10× and 20×, power between 42 and 44, aperture between 4 and 13 and speed between 16 and 25 for acquisition of vascular cells in N. cadamba. Utilizing this protocol, high quality of RNAs were extracted, with RIN(RNA integrity number)value of 5.0, 6.6 and 7.9 in cambium cells, phloem cells and xylem cells, respectively. These RNAs can be used for subsequent RNA-seq analysis. Accordingly, the vascular tissue cells capture system by LMD was established in N. cadamba, which would be of value for revealing the regulatory mechanism of vascular tissue division and differentiation in N. cadamba, and provide a reference for capturing vascular cell in other forest tree species.]]> 2021/8/31 16:11:24 1,2, LONG Jianmei^1,2, DONG Tiantian^1,2, ZHENG Danjing^1,2, ZHANG Liding^1,2, PENG Changcao^1,2*]]> WANG Xue^1,2, LONG Jianmei^1,2, DONG Tiantian^1,2, ZHENG Danjing^1,2, ZHANG Liding^1,2, PENG Changcao^1,2* 14true Ananas comosus var. bracteatus, Hiseq2500 sequencing and SMRT the third-generation full-length transcriptome sequencing were applyed to identify lncRNA of Ananas comosus var. bracteatus. The results were as follows:(1)A total of 6 018 lncRNA were identified, containing 3 298 intergenic lncRNA, 870 antisense lncRNA, 717 intron lncRNA and 1 109 sense lncRNA, which were greatly improved compared with the second-generation information.(2)Structural analysis showed that the overall expression level of lncRNA was lower than that of mRNA. The transcript length distribution of lncRNA in the range of 400-1 200 nt was higher than that of mRNA, while in the range > 1 600 nt, the proportion of lncRNA distribution was significantly lower than that of mRNA. The number of exons in lncRNA was generally less than that of mRNA, and the open reading frame was also shorter in length than that of mRNA.(3)For analysis of differential expression, 1 710 differentially expressed lncRNA were identified during the development of complete green and complete white leaves.(4)Target gene prediction results showed that 5 441 lncRNA were predicted target genes by Cis action, and 1 544 lncRNA were predicted target genes by Trans action.(5)Functional annotation and enrichment analysis of target genes revealed that the target genes of differentially expressed lncRNA mainly act on metabolic activities and signal transduction of leaves as enzyme proteins, and were closely related to leaf color formation, photosynthesis and leaf growth. The lncRNA information identified in this paper and, as well as the analysis of its structure and functions, provide the data basis for the study of the epigenetic regulation mechanism of lncRNA in Ananas comosus var. bracteatus and other plants in Bromeliaceae. The identified differentially expression of lncRNA plays an important role in the chimeric character formation and development of leaf of Ananas comosus var. bracteatus.]]> 2021/8/31 16:11:24 1, LIN Zhen¹, XUE Yanbin¹, MAO Meiqin¹, XIANG Yixuan¹, LIU Jiawen¹, ZHOU Xuzixin¹, MA Jun^1,2*]]> HU Hao¹, LIN Zhen¹, XUE Yanbin¹, MAO Meiqin¹, XIANG Yixuan¹, LIU Jiawen¹, ZHOU Xuzixin¹, MA Jun^1,2* 13true Magnolia officinalis is a famous traditional medicinal plant, belonging to the Magnoliaceae family and Magnolia L. genus and being widely cultivated in China. Its barks, root barks, branch barks, leaves, flowers and fruits could be used as medicine or food. However, the whole genome information is little known for this plant species. In order to obtain the whole genome sequence information of M. officinalis, the leaf DNA was used as the material, and the third-generation sequencing technology of Pacbio Sequel was used to establish its nucleotide sequence database. Then genome assembly, function annotation and evolution analysis were carried out by bioinformatic methods. The results were as follows:(1)140.91 Gb the third-generation data were obtained after the original sequencing data, with the Read N50 about 13 784 bp. The assembled M. officinals genome size was 1.68 Gb, contig N50 being about 222 069 bp, and the integrity of single-copy gene being 81.0%.(2)98.40% of the genes from the assembled sequence got gene annotation after being compared with functional databases such as NR, KOG and KEGG. The result of KOG gene annotation was that the protein function of M. officinalis concentrated in the general functional prediction only, posttranslational modification, protein turnover, chaperones signal transduction mechanisms. GO functional classification indicated that the genes of M. officinalis concentrated on cell components and biological processes. KEGG analysis found that the M. officinalis genes mostly involved in metabolic pathways.(3)By comparative genomics analysis, the genomes of Vitis vinifera, Arabidopsis thaliana, Oryza sativa, Poplar trichocarpa, Ginkgo biloba, Amborella trichopoda, Camellia sinensis and Cinnamomum kanehirae were aligned. It was found that 20 801 of 23 424 genes in M. officinalis could be classified into 12 129 families, 515 gene families being unique to M. officinalis. The genetic evolution tree constructed from the genomes of the selected reference species pointed that the M. officinalis(Magnoliaceae)was closely related to Cinnamomum kanehirae(Lauraceae), and the divergence time between the two species was about 122.5 mya. It is the first time to use the third-generation sequencing technology to analyze the whole genome of M. officinalis in the study, which is conducive to its further development and utilization, and also provides the information for the study of the whole genome of other medicinal plants.]]> 2021/8/31 16:11:24 *]]> YIN Yanpeng, DING Qiaojiao, LUO Jiawei, LIN Xinna, ZHANG Min, PENG Cheng, GAO Jihai^* 12true Curcuma were collected. After DNA extraction, PCR amplification and sequencing, seven kinds of DNA barcoding sequences, including ITS, ITS2, matK, psbA-trnH, trnL-trnF, rpoB and atpB-rbcL, were firstly compared in terms of success rate of PCR amplification and characteristics of sequences. Then, by means of variation site analysis and genetic distance calculation, these seven kinds of DNA barcoding sequences were further evaluated. Finally, the unidentified samples were identified by the phylogenetic tree based on the chosen DNA barcoding sequences. The results were as follows:(1)ITS, ITS2 and matK barcoding sequences were inapplicable due to the low success rate of PCR amplification and sequencing; The variation information of psbA-trnH, trnL-trnF and rpoB was insufficient to distinguish three different original species of Curcumae Rhizoma; Only atpB-rbcL barcoding sequence was 642-645 bp in length with 29.0%-29.9% GC content and 11 variation sites, for which, the three species of Curcumae Rhizoma could be distinguished only by atpB-rbcL barcoding sequence.(2)According to the phylogenetic tree based on atpB-rbcL sequence, the unidentified samples were identified as Curcuma wenyujin. In conclusion, atpB-rbcL sequence could be used as a standard sequence for the rapid and efficient identification of original species of Curcumae Rhizoma.]]> 2021/8/31 16:11:24 1, LIU Yang², LIU Peiwei¹, FU Chuankun¹, LU Lilan¹, WEI Jianhe^1,2*]]> ZHANG Yuxiu¹, LIU Yang², LIU Peiwei¹, FU Chuankun¹, LU Lilan¹, WEI Jianhe^1,2* 11true Pinus caribaea×P. elliottii(C×E), P. elliottii×P. caribaea(E×C)and P. elliottii×P. taeda(E×T)were better than or close to half-sib controls, and P. massoniana×P. elliottii(M×E), P. massoniana×P. taeda(M×T), P. massoniana×P. caribaea(M×C), P. taeda×P. caribaea(T×C), P. taeda×P. massoniana(T×M)and P. taeda×P. elliottii(T×E)were much lower than those of half-sib controls. According to the five fertility indexes, the overall performance of hybridization in the subgroup was better than that of intersubgroup hybridization, and that of the three hybrids of P. elliottii and P. caribaea as female parent was better than that of the other six hybrids of P. massoniana and P. taeda as female parent. In addition to P. taeda×P. elliottii(T×E), the living hybrid offspring were obtained in all the nine hybrids, especially the living hybrid offspring of P. massoniana and three kinds of foreign pines were obtained for the first time in relevant studies. It is of great significance to the study of interspecific hybridization breeding of pine trees.]]> 2021/8/31 16:11:24 1, FENG Yuanheng², JIA Jie², YANG Zhangqi^1,2,3,4*]]> SUN Mingsheng¹, FENG Yuanheng², JIA Jie², YANG Zhangqi^1,2,3,4* 10true Lilium davidii var. unicolor scales and to solve the problems of insufficient provenance and long breeding cycle, we selected scales of L. davidii var. unicolor as material, fully randomized block design using two factors(temperature and scale layer), with temperature of 20, 25 and 30 ℃, scale layers of outer layer, middle layer and inner layer. Suspected incidence of scales, differentiation rate and number of small bulb were analyzed. Results showed that there were significant or extremely significant differences in the effects of different temperature treatments and layers on the suspected incidence, differentiation rate and small bulb differentiation of scales. The results were as follows:(1)The higer the temperature, the lower the suspected incidence of scales. Suspected incidence rate was the highest(38.67%)under 20 ℃ treated after two weeks, 30 ℃ the lowest(10%). Every layers of suspected incidence from high to low was outer layer > middle layer > inner layer.(2)Scale differentiation rate was the highest under 25 ℃ and 30 ℃ treated after six weeks which was 91.33% and 90.89%, respectively. The differentiation rates of small bulbs on middle and inner scales were significantly higher than that on outer scales.(3)The number of small bulbs formed on scales was the largest under 30 ℃ treatment after six weeks, which was 2.00 per scale. Meanwhile, the number of small bulbs on middle and inner scales were significantly higher than that on outer scales. It is suggested that middle scale(3-4 layers), inner scale(5-7 layers)cultivate temperature was the best condition for L. davidii var. unicolor scales propagation by substrate embedding under 25-30 ℃.]]> 2021/8/31 16:11:24 *, JU Xiuting, TANG Daocheng, LÜ Chunna]]> JIA Rulong, TANG Nan^*, JU Xiuting, TANG Daocheng, LÜ Chunna 9true Tuber is a type of fungus underground, formed by infecting suitable plant roots. And some species such as T. melanosporum and T. indicum are rare and valuable for both medicine and food, and have high economic values. The over excavation of Tuber has caused a shortage of wild Tuber resources, and the artificial cultivation technology of Tuber is an important foundation for the sustainable development of the Tuber industry. At present, the ascosporic suspension made from the homogenate of the fruiting body, is mainly used in the artificial cultivation, which is symbiotic with the roots of the planting seedlings. It is valuable for reducing the dependence on the fruiting body of Tuber and the production cost, protecting the wild resources of Tuber under the forest and promoting the process of artificial propagation of Tuber, to use the hyphae of Tuber instead of the spore suspension for inoculation, but it needs mature and efficient technology for hyphae culture. Tuber samples in this study were taken from the forests of Yunnan, Shaanxi and other places. Then, ITS sequence analysis was carried out to identify species and purify hyphaes. The most suitable hyphae growth medium was selected by the L₉(3⁴)orthogonal test(four factors with three levels), using the hyphae diameter as an index. The results were as follows:(1)The 13 Tuber samples identified by morphological and ITS molecular identification belonged to T. indicum and T. pseudoexcavatum.(2)The optimum carbon source for T. indicum hyphae was potato extract, and the optimum nitrogen source was yeast extract. However, VB1 had no significant promotion effect on the hyphae growth of T. indicum.(3)The optimal combination of carbon, nitrogen and inorganic salts was potato extract 150 g·L^-1, yeast extract 3 g·L^-1, MgSO₄ 2 g·L^-1, and KH₂PO₄ 2 g·L^-1. The diameter of hyphae can reach 49.44 mm, which is cultured at 25 ℃ and natural pH for 8 d.]]> 2021/8/31 16:11:24 1, ZHANG Guozhen², ZHANG Bingbing¹, XU Ying¹, QIN Xiaobo^1,2*]]> FAN Lijuan¹, ZHANG Guozhen², ZHANG Bingbing¹, XU Ying¹, QIN Xiaobo^1,2* 8true Michelia species(Michelia macclurei, Michelia ‘Danxia', M. crassipes, Michelia ‘Yuxia' and M. yunnanensis)and evaluate their cold-resistance according to the leaf anatomy, nine structural indicators such as thickness were measured at three temperature nodes(normal temperature, natural cooling and low-temperature wintering). We selected cold-resistant structural indexes by clustering analysis and principal component analysis, and used the membership function method to evaluate the cold-resistance of five Michelia species. The results were as follows:(1)Cold-resistant structural indexes selected by cluster analysis at different cooling stages were different. At normal temperature, the ratio between palisade tissue and spongy tissue(P/S), sponge tissue(TS)and lower epidermis thickness(TL)were representative indicators; at natural cooling stage, P/S, TS and cuticle thickness(CT)were representative indicators; and in the low-temperature over wintering period, thickness of palisade tissue(TP), TS and thickness of upper epidermis(TU), and the orders of cold-resistance in three periods were completely different according to membership function analysis.(2)The indicators selected by principal component analysis were TU, P/S and looseness of palisade tissue(SR). The cold-resistance of five Michelia species evaluated by membership function was M. macclurei >M. crassipes >Michelia ‘Danxia' > M. yunnanensis > Michelia ‘Yuxia'. The results were consistent with the field observation and physiological and biochemical evaluation results. It could be concluded that clustering analysis on screening cold-resistant structural indexes and evaluate cold-resistance based on the leaf structure indicators at a single period had certain defects, while the principal component analysis on evaluating cold-resistance by screening indexes had more reference value.]]> 2021/8/31 16:11:24 *, YU Qiuxiu, SUN Lingxiao]]> TAN Yinyin, JIN Xiaoling^*, YU Qiuxiu, SUN Lingxiao 7true Rhododendron to adapt to environmental factors in different seasons. Rhododendron delavayi, widely introduced and cultivated, was planted in a 2-gallon plastic basin in the open field. The dynamic variations of photosynthetic characteristics and influencing factors in different growth phases were investigated by the technique of gas exchange and chlorophyll fluorescence. The results were as follows: The growth cycle of R. delavayi were almost overlaps with photosynthetic variation at Kunming area. The variations of photosynthetic characteristics reflected the variations of growth cycle. In the net early growth period from May to July, the photosynthetic rate(P_n)increased rapidly, the stomatal conductance(G_s)was the decisive factor affecting P_n. The relative humidity of air(RH)had a significant indirect effect on P_n by affecting G_s and transpiration rate(T_r). The P_n and growth could be stimulated by increasing RH at this phase. In the peak growth period from July to October, the RH, P_n and G_s of R. delavayi were high and stable. However, lower air temperature(AT)in this phase was a unique climate character in Kunming area. Low AT became an important factor affecting photosynthetic capacity at this phase, and increasing AT could improve P_n and promote the growth of R. delavayi. In the late growth phase after October, AT and RH decreased rapidly, stomata closed gradually, the maximum quantum efficiency of photosynthetic system Ⅱ(F_v/F_m)and P_n were declined. G_s and F_v/F_m were the main factors affecting photosynthesis at this stage. Our results can provide important references for the scientific cultivation and popularization of R. delavayi.]]> 2022/5/8 9:42:48 1,2,3, PENG Lvchun^1,2,3, SONG Jie^1,2,3, ZHANG Lu^1,2,3, XIE Weijia^1,2,3, QU Suping^1,2,3*]]> CAI Yanfei^1,2,3, PENG Lvchun^1,2,3, SONG Jie^1,2,3, ZHANG Lu^1,2,3, XIE Weijia^1,2,3, QU Suping^1,2,3* 6true Solanum diphyllum is a kind of small shrub of Solanaceae, and little has been known about its Cd tolerance ability in the existed researches so far. In order to investigate the physiological responses, growth responses and Cd tolerance characteristics of S. diphyllum seedlings, we conducted a hydroponic experiment with 160 μmol·L^-1 Cd stress to determine the indexes of growth(taproot, height, stem diameter, leaf number, biomass, and root to shoot ratio), tolerance index(TI), Cd content, Cd accumulation characteristics, SOD activity, osmotic adjustment substances(MDA, free proline and soluble sugar), and photosynthetic pigment(Chlorophyll a, Chlorophyll b and carotenoid)in different stress time(0, 0.5, 1, 3, 7, 19 d). The results were as follows:(1)Compared with controls, Cd had no significant effects on the growth of S. diphyllum seedlings when stress time was 0-3 d; Obvious Cd toxicity was aggravated with the prolonging of Cd stress time(3-19 d); During the period of experiment, Cd stress reduced the total biomass of S. diphyllum seedlings by 13.28%-62.40%, root suffered the most serious biomass damage among different plant tissues.(2)The maximum Cd accumulation of the whole plant was 60.14 μg·plant^-1, for root and shoot the maximum Cd accumulation were 43.49 μg·plant^-1 and 16.65 μg·plant^-1 respectively, Cd accumulation of shoots accounted for 15.46%-35.24% of the whole plant.(3)MDA content of S. diphyllum seedlings increased with the increase of stress time, the maximum increase of MDA content in roots was 5.25 times that of control; SOD activity, contents of free proline and soluble sugar, as well as photosynthetic pigment contents increased at the beginning and then decreased.(4)Tolerance index(TI)showed a different tendency, it decreased with the increase of stress time, and the minimum value of S. diphyllum seedlings during the whole period of experiment was 0.64. The above results indicate that S. diphyllum seedlings have a certain degree of tolerance to high concentration of Cd stress(160 μmol·L^-1), which suggests that it is a potential Cd tolerance plant. The osmotic adjustment and root retention might be the main methods of S. diphyllum seedlings to alleviated toxicity to plant growth caused by Cd. This research provides a theoretical basis for finding suitable phytoremediation materials.]]> 2022/5/8 9:42:48 *, ZHAO Yin, LIU Sili, JIA Yin, JIANG Beibei, WU Mengxi, ZHANG Lu, XU Qian, WANG Kailu, ZENG Yong]]> ZHOU Jiao, PAN Yuanzhi^*, ZHAO Yin, LIU Sili, JIA Yin, JIANG Beibei, WU Mengxi, ZHANG Lu, XU Qian, WANG Kailu, ZENG Yong 5true Pueraria montana var. thomsonii)and ‘Guige 8'(P. montana var. lobata)as materials, we determined the agronomic traits, the content of amylose and amylopectin, and the key enzyme activity of starch synthesis, and analyzed their relationship of dynamic changes. The results were as follows:(1)During the development of root tubers, the activity changes of ADP-glucose pyrophosphorylase(AGPase), soluble starch synthase(SSS), granule-bound starch synthase(GBSS)and starch branching enzyme(SBE)in two varieties of P. lobata showed a single peak curve that first increased and then decreased, it was basically consistent with the trend that the contents of amylose, amylopectin and total starch increased from slow to rapid and then stabilizing, that was, from formation period to expansion period, they increased gradually; they reached the maximum in the later expansion stage and declined rapidly in a later; to the maturity stage, they declined gradually and maintained at a higher level.(2)The yield and starch content of ‘Guige 8' were significantly higher than those of ‘Guige 1', the enzyme activity of ‘Guige 8' was also significantly higher than that of ‘Guige 1'.(3)The average length and width of root tubers, fresh weight per plant, dry matter content and yield showed a trend of “slow-fast-stable”, and starch content showed similar changes.(4)Correlation analysis showed that four key enzyme activities of starch synthesis were significant or highly significant positively correlated with the contents of amylose, amylopectin and total starch and the average length of root tubers; they were positively correlated with the fresh weight per plant, yield, dry matter content and the width of root tubers, but the difference was not significant. On the whole, the increase of starch content is the result of swelling and thickening of Kudzu root. Improving the enzyme activities of AGPase, SSS, GBSS and SBE and the ability to compete for substrates can improve the accumulation of Kudzu root starch and its components, thereby improving the yield.]]> 2022/5/8 9:42:48 1,2, YU Jianbin¹, XIAO Dong¹, HE Longfei¹, WANG Aiqin^1*]]> GUO Lijun^1,2, YU Jianbin¹, XIAO Dong¹, HE Longfei¹, WANG Aiqin^1* 4true Dalbergia odorifera seedlings under different temperatures. The effects of different exogenous melatonin concentrations(300, 500, 600, 700, 900, 1 200 μmol·L^-1)on the eco-physiological traits in term of plant growth, photosynthesis, leaf pigment content, leaf water status, membrane system in D. odorifera seedlings under ambient temperature(28 ℃ day / 25 ℃ night)and low temperature(8 ℃ day /5 ℃ night)were studied. The optimum concentration of melatonin, which could promote the growth and development and enhance the low temperature tolerance, was screened out through the comprehensive evaluation of subordinate function analysis. The results were as follows:(1)Application of all exogenous melatonin concentrations could increase the growth and development of D. odorifera(morphology, stem height and plant height), the photosynthetic parameters(P_n, G_s, C_i, and T_r), the pigment contents(Total Chl, Chl a, Chl b, and Caro), and soluble protein content under ambient temperature.(2)Under low temperature stress, spraying different concentrations of melatonin solution could alleviate the negative impacts caused by low temperature on morphology, stem height increment and plant height increment, P_n, C_i, T_r, Chl, Chl a, Caro, dew point water potential(DWP)and relative conductivity, malonaldehyde and soluble protein contents.(3)The results of subordinate function analysis showed that 600-700 μmol·L^-1 melatonin had the best effects on promoting growth and development and alleviating chilling damages. This study provides scientific guidance for coping with chilling stress on D. odorifera seedlings and popularizing its cultivation in low temperature regions of subtropical areas.]]> 2022/5/8 9:42:48 1, ZHANG Yixuan¹, MIAO Lingfeng^1*, YANG Fan^1,2,3]]> PU Yujin¹, ZHANG Yixuan¹, MIAO Lingfeng^1*, YANG Fan^1,2,3 3true 2022/5/8 9:42:48 *, PAN Jiechun, XU Jiongzhi, ZHAO Rongmei, WU Shiyan, MAO Junru, LIAO Tianlan, CHEN Yunjie, DING Chengpei, WU Yu, HUANG Junhao, HU Guorui]]> HE Jia'nan, PENG Jiechun, DENG Yingyi^*, PAN Jiechun, XU Jiongzhi, ZHAO Rongmei, WU Shiyan, MAO Junru, LIAO Tianlan, CHEN Yunjie, DING Chengpei, WU Yu, HUANG Junhao, HU Guorui 2true Lycium ruthenicum to salt-drought mixed stress, we used pot culture test method to study the effects of salt and simulated drought interaction experiments on seedling growth, and observe the physiological responses by spraying different concentrations of salicylic acid(SA)that improve the survival rate of L. ruthenicum seedlings under salt-drought stress. The results showed that the contents of soluble sugar, soluble protein and proline of L. ruthenicum leaves were significantly increased from control treated with exogenous SA under salt-drought mixed stress, the malondialdehyede(MDA)content decreased(P<0.05), whereas the activities of catalase(CAT), peroxidase(POD), and superoxide(SOD)increased, among all concentrations, 0.5 mmol·L^-1 SA treatment had better effect than 0.1 mmol·L^-1 SA. To sum up, L. ruthenicum has a certain ability to adapt mild salt-drought stress, and moderate concentrations of SA can improve the osmotic regulatory substances and antioxidant enzymes activities in the leaves under salt-drought environment. The study results can provide the theoretical bases for deeply understanding the growing of L. ruthenicum under different stresses.]]> 2022/5/8 9:42:48 1, LI Yongjie², LI Jin^1*]]> MA Yonghui¹, LI Yongjie², LI Jin^1* 1true