Kandelia candel, Bruguiera gymnorrhiza and mixed forests in Zhenzhu Gulf, Beilun River of Guangxi. The results were as follows:(1)The SOC storage of Kandelia candel, Bruguiera gymnorrhiza and mixed forests were 140.73, 124.94, 144.71 t·hm^-2, respectively, and there was no significant differences among them(P<0.05); The SOC storage of Bruguiera gymnorrhiza and mixed forests from the highest to the lowest invertical distribution was 20-40 cm > 0-20 cm > 40-60 cm, and the SOC storage of Kandelia candel decreased with the increase of soil depth.(2)TN storage of Kandelia candel, Bruguiera gymnorrhiza and mixed forests were 6.49, 5.01, 5.87 t·hm^-2, respectively, decreasing with the increase of soil depth.(3)There was a significant correlation between SOC andTN storage in Kandelia candel, Bruguiera gymnorrhiza and mixed forests(P<0.01), and correlation coefficients were 0.924, 0.971 and 0.844, respectively, which indicated that there was coupling effect between SOC and TN.(4)The C/N of three stands ranged from 16.77 to 24.39, indicating that the organic matter mainly came from land. There was a significant correlation between soil C/N and SOC storage in Bruguiera gymnorrhiza and mixed forests(P<0.05), but there was no significant correlation between C/N and TN storage in the three stands.(5)The soil carbon storage of three mangrove stands was higher than the average value of forest soil carbon storage in China, and there was extremely significant correlation between SOC and TN.]]> 2020/4/13 11:14:55 TAO Yuhua, HUANG Xing, WANG Xueping, ZHONG Qiuping 17true Caenorhabditis elegans screening models. The results were as follows:(1)A total of 26 marine actinobacteria were isolated from 21 samples of seven associated mangrove plants, belonging to nine families and fifteen genera consist of Nocardiopsis, Brachybacterium, Curtobacterium, Demequina, Gordonia, Nocardioides, Lysinimicrobium, Microbacterium, Pseudonocardia, Micrococcus, Promicromonospora, Amycolatopsis, Yimella, Kitasatospora and Streptomyces, and the dominant genus was Streptomyces.(2)Two kinds of actinobacteria were found to have anti-aging effect by Caenorhabditis elegans screening models. All the above results indicate that there are abundant medical actinobacteria resources in the west coast of Hainan, and the study provides research fundamental data for anti-aging research of marine actinobacteria.]]> 2020/4/13 11:14:55 *]]> LI Mi, YI Xiangxi, YANG Caini, PANG Xiaoting, JIANG Shu, GAO Chenghai^* 16true Caenorhabditis elegans screening model were used to explore the diversity and nematicidal activity of endophytic bacteria isolated from mangrove plants. The results were as follows:(1)A total of 33 endophytic bacteria were isolated by 16 organs and tissues which could be classified into 19 families and 23 genera and the dominant genus were Bacillus, and ten potential new species or genera were obtained.(2)Through the nematicidal activity test, two kinds of bacteria with nematicidal activity were obtained. Two endophytic bacteria IMDGX 4725 and IMDGX 4744 had the extremely significant nematicidal activities and the lethal concentrations of 50% were 61.58 and 100.89 mg·mL^-1, respectively. The research confirm that mangrove plants collected from Xuwen are rich in endophytic bacteria. At the same time, parts of the bacteria have strong nematicidal activity, which could be a potential source for the discovery of the new microbial insecticides.]]> 2020/4/13 11:14:55 *]]> LI Mi, HOU Shishi, YIN Jianglin, HE Yuming, LIU Yonghong, YI Xiangxi^* 15true Caenorhabditis elegans screening models. The results were as follows:(1)The 32 marine bacteria were obtained from 46 tissues of true mangrove plants without repeatitions. The 32 bacteria to comparison of 16S rRNA gene sequences which could be classified into 12 families and 17 genera. The dominant genus were Bacillus. It was discovered that a novel strain of suspected genus Aurantimonas, which 16S rRNA gene sequence similarities were less than 97%;(2)The three cultured marine bacteria could significantly delay Caenorhabditis elegans developing and had dominant anti-aging activities(P<0.05). All the above results indicate that the true mangrove plants collected from the west coast of Hainan have high species diversity and part strains are rich in anti-aging activity.]]> 2020/4/13 11:14:55 *]]> LI Mi, GAO Chenghai, JIANG Shu, WEI Juan, LIU Siping, DAI Enzhi, YI Xiangxi ^* 14true Caenorhabditis elegans model. The results were as follows:(1)A total of 22 strains of actinomycetes were isolated, belonging to four orders, seven families and nine genera consist of Amycolatopsis, Curtobacterium, Demequina, Isoptericola, Lysinimicrobium, Microbacterium, Rhodococcus, Sinomonas, Streptomyces, and Streptomyces was the dominant flora of this study with eleven strains isolated, and five strains of IMDGX 6012, IMDGX 6028, IMDGX 6118, IMDGX 6326, IMDGX 6119 were identified as potential new species actinobacteria. The highest similarities among the strains and the effective published strains Amycolatopsis lexingtonensis, A. niigatensis, Curtobacterium albidum, C. cit-reum, Demequina salsinemoris were 97.75%, 98.15%, 98.32%, 98.44%, 98.45%, respectively. The above five rhizosphere actinobacteria belong to rare actinobacteria and were preliminarily identified as potential new species.(2)The results of anti-aging of fermentation products showed that the metabolites of eight strains of actinobacteria could signifi-cantly prolong the lifespan of nematodes(P<0.05), including one strains of Curtobacterium, one strains of Demequina, one strain of Sinomonas and five of which were from Streptomyces, indicating that Streptomyces had potential to produce anti-aging active substances. IMDGX 6028 and IMDGX 6118, as potential new species of Amycolatopsis and Curtobacte-rium, had extremely significant anti-aging activity(P<0.01). When the crude metabolite concentration was 500 μg·mL^-1, the survival time of C. elegans was increased by 22.2% and 26.6%, respectively, compared with the blank group. The results indicate that there are abundant resources of culturable actinobacteria and the potential to discover new species and anti-aging activity strains in the true mangrove rhizosphere soils in the west coast of Hainan.]]> 2020/4/13 11:14:55 *]]> HOU Shishi, LI Mi, JIANG Shu, HAN Minmin, LIU Yonghong, YI Xiangxi^* 13true Caenorhabditis elegans screening models. The results were as follows:(1)A total of 24 strains of actinobacteria were isolated from 46 plant tissues of true mangrove plants and distributed in eleven genera, seven families. Nine strains belonged to Streptomyces. Moreover, IMDGX 6270, IMDGX 6137 and IMDGX 6173 were three suspected potential new strains. The number of strains isolated from each sample varied greatly. Among them, the leaves had the largest number of strains and the richest diversity(18 strains). Next, eight strains were obtained from the stem. The number of roots was four, which may be related to the large number of stem and leaf tissue samples, and the number and diversity of strains isolated from M5 medium were the highest, while the number and diversity of strains isolated from M11 medium were the lowest. The results showed that the isolation medium with different nutrients had a great influence on the growth of microorganisms.(2)In addition, four strains of actinobacteria were screened by Caenorhabditis elegans model and showed anti-aging activity, and the survival time was increased by 17.16%, 28.11%, 29.05% and 27.10%, respectively, compared with the blank group. This indicates that there may be abundant actinobacteria resources in the mangrove plants in the west coast of Hainan, which can provide a new source for the research and development of anti-aging drugs.]]> 2020/4/13 11:14:55 *]]> JIANG Shu, LI Mi, HOU Shishi, HAN Minmin, YIN Jianglin, GAOChenghai^* 12true Aegiceras corniculatum collected from Beihai City, and studied the anti-thrombotic activities of those strains. The endophytic and rhizospheric bacteria were isolated from A. corniculatum by using six different isolation media. The anti-thrombotic activities of all species were screened out by thrombolysis experiment in vitro. The results were as follows:(1)Based on the phylogenetic analysis of 16S rRNA gene sequence, a total of 125 strains were obtained from the tissue and rhizosphere soil of A. corniculatum. They were classified into 74 species, 39 genera, 27 families and 3 phylas of Proteobacteria, Actinobacteria and Firmicutes. Bacillus sp. was the dominant genus, and the total rate was 13.5%.(2)The results of anti-thrombotic activity test showed that 18 strains with anti-thrombotic activity were screened out, and the total positive rate reached to 24.32%. The activity of the strains was further verified by the re-screening and re-verification test. The strains of B1850, B1989 and B2632 with strong anti-thrombotic activity were obtained. To sum up, there are abundant culturable bacterial resources in Aegiceras corniculatum in Beihai of Guangxi, which could be a potential source for the discovery of new plasminogen and thrombolytic drugs.]]> 2020/4/13 11:14:55 1, HUANG Shushi², HU Wenjin ³, LI Zhe², HUANG Yuanlin², WANG Qiaozhen², PAN Xinli^2*]]> LI Fei¹, HUANG Shushi², HU Wenjin ³, LI Zhe², HUANG Yuanlin², WANG Qiaozhen², PAN Xinli^2* 11true Acrostichum aureum, Aegiceras corniculatum, Acanthus ilicifolius, Bruguiera gymnorr-hiza, Kandelia candel)and the four semi-mangrove speices(Heritiera littoralis, Hibiscus tiliaceus, Pongamia pinnata, Thespesia populnea)at Mangrove Natural Reserve of Qi'ao Island of Zhuhai City, Guangdong Province, China. We observed leaf anatomical structures including leaf epidermis and ancillary structure, hypodermis, palisade tissue, spongy tissue and stomata, and then measured stomatal density and leaf thickness under a JSM-6360LV scanning electron microscope. We measured leaf functional traits including specific leaf area, leaf mass per area, leaf water content, and leaf fresh/dry weight ratio according to a published standar-dized protocol. The results were as follows:(1)Leaves of all the nine mangrove species had common anatomical characteristics such as epidermis covered by horny layers, palisade and spongy tissues that differentiated in the mesophyll, and sunken stomata.(2)Leaves of the true mangrove species had waxy layers and hypodermis, but had no foliar trichomes, and their stomata were only distributed in the lower epidermis; however, leaves of semi-mangrove speices had less wax layers, some of them had foliar trichomes but no hypodermis, and distribution of stomata in either upper or lower epidermis was not consistent.(3)Stomatal density and specific leaf area of true mangrove species were significantly lower than those of semi-mangrove speices(P<0.05), while leaf thickness, leaf water content, specific leaf mass and leaf fresh/dry weight ratio were significantly higher than those of semi-mangrove speices(P<0.05). These results indicate that leaves of true mangrove species have more advantages in maintaining salinity balance and water conservation than semi-mangrove speices, and thus can better adapt to high salinity in intertidal environments.]]> 2020/4/13 11:14:55 1,2, CAI Shuihua⁴, TAN Shujuan^1,2, YE Wanhui^1,3, SHEN Hao^1,3*]]> HUANG Yiyi^1,2, CAI Shuihua⁴, TAN Shujuan^1,2, YE Wanhui^1,3, SHEN Hao^1,3* 10true Laguncularia racemosa on the native species, it was observed that the effects of three concentrations(0.1, 0.3, 0.5 g·mL^-1)of aqueous extracts from L. racemosa roots and leaves on the germination, seedling growth and leaf antioxidase activity of the two native mangrove species Aegiceras corniculatum and Rhizophora apiculata. The results were as follows:(1)The aqueous extracts of Laguncularia racemosa had inhibitory effects on seedling rate of seeds, germination index and root length of Aegiceras corniculatum, in which the inhibitory effects on root length increased with the concentration of aqueous extracts increasing.(2)In addition, effects of root aqueous extracts on the growth indexes of root length, seedling height and biomass of A. corniculatum seedlings were positive in lower concentration and negative in higher concentration.(3)Meanwhile, the aqueous extracts of Laguncularia racemosa had promoting effects on the germination rate, germination index and growth index of Rhizophora apiculata hypocotyls. Bud length in the groups treated by the root aqueous extracts of 0.1 and 0.3 g·mL^-1 and biomass in the groups treated by the root and leaf aqueous extracts of 0.1 and 0.3 g·mL^-1 were significantly larger than those in control. The aqueous extracts of Laguncularia racemosa had promoting effects on the biomass of the Rhizophora apiculata seedling as well.(4)In stress physiology, the SOD activities of Aegiceras corniculatum and Rhizophora apiculata seedlings decreased with the concentration of aqueous extracts increasing, while and the POD activities of R. apiculata seedlings in treatment group with 0.3 g·mL^-1 root aqueous extract and in the group with 0.1 g·mL^-1 leaf aqueous extract were significantly higher than those in control. The results indicate that the sensitivities of the two native species to the allelopathy of Laguncularia racemosa are different. The aqueous extracts have inhibitory effect on the growth of Aegiceras corniculatum, while have promotional effect on the growth of Rhizophora apiculata to some extent.]]> 2020/4/13 11:14:55 *, WANG Bingyu, WANG Xuping, Juma GUL]]> YANG Shan, LIU Qiang^*, WANG Bingyu, WANG Xuping, Juma GUL 9true Clerodendrum inerme is a semi-mangrove plant that is an important part of island and coastal shelter forests. Fresh water and soil are important ecological factors for island vegetation restoration. In order to summarize the most suitable soil, water and fertilizer treatment for C. inerme, we set different matrices, irrigation frequencies and nitrogen treatments, and studied the biomass and water use efficiency of C. inerme under the different treatments. The results were as follows:(1)Peat-containing mixed matrix had a prominent effect on the biomass accumulation of C. inerme. The underground biomass(Square Root Calculations)of S₄ matrix(coral sand:peat:red soil:coco peat=12:2:3:3)was 1.66 g, which was significantly higher than that of peat-free matrix, and the total biomass(Square Root Calculations)of S₁ matrix(coral sand:peat=3:2)was 4.54 g, which was significantly higher than other matrices.(2)In addition, the total biomasses treated at medium and low irrigation frequencies were 4.02 and 4.23 g, respectively, which were significantly higher than 3.46 g at high irrigation frequency. But the biomass between different nitrogen treatments was not significant, indicating that C. inerme could obtain high biomass under medium and low irrigation frequencies, with less or no nitrogen fertilizer.(3)For water use efficiency, the original coral sand matrix was kept at a low level, and the mixed matrix has been significantly improved, especially at medium and low irrigation frequencies. Therefore, C. inerme can grow well under peat-containing matrix, medium and low irrigation frequency, with low nitrogen or no nitrogen.]]> 2020/4/13 11:14:55 1,3, ZHANG Wanwan², LI Shiyu^1,3, WANG Faguo¹, SHENG Aiwu², LIU Dongming^1*]]> NIE Liyun^1,3, ZHANG Wanwan², LI Shiyu^1,3, WANG Faguo¹, SHENG Aiwu², LIU Dongming^1* 8true Tournefortia argentea is an evergreen plant of Boraginaceae with important ecological, ornamental and edible values. It is a pioneer species at the tropical coastal and island of the Eastern Hemisphere. In order to provide basic data for protection and utilization of T. argentea, and to understand the ecological adaptation mechanism on tropical coral islands, we selected individuals of T. argentea naturally growing on Dongdao Island of Xisha Islands to examine their morphological and physiological characteristics, nutrient composition, and the physical and chemical properties of their rhizosphere soil. The results were as follows:(1)T. argentea was a heliophyte, characterized with low stomatal density, small specific leaf area, well developed spongy tissue, large ratio of branch to cavity, which help T. argentea to keep moisture and obtain water effectively.(2)Thick white hair on leaf surface of T. argentea could reflect strong light, decrease water evaporation, and was helpful for it to acclimatize the environments of strong light and drought.(3)The content of proline in leaves of T. argentea was high, but the content of malondialdehyde was low, indicating that T. argentea had good drought resistance.(4)The content of nutrient elements were high in plant leaves but low in rhizosphere soil, suggesting that T. argentea was able to use nutrient elements of rhizosphere soil effectively and had strong tolerance to barren soil. The branches were fragile and easy to break, which helped T. argentea in preventing from being uprooted by typhoons. Moreover, the branches were water-storing tissues so after typhoon the crown could be recovered quickly. In a word, T. argentea can adapt to various environmental stresses, such as strong light, gale, drought and barren soil. Therefore, T. argentea can be used as an important plant species of vegetation restoration in tropical coral islands.]]> 2020/4/13 11:14:55 1,2, LIU Nan¹, WEN Meihong^1,2, REN Hai¹, JIAN Shuguang^1*]]> CAI Hongyue^1,2, LIU Nan¹, WEN Meihong^1,2, REN Hai¹, JIAN Shuguang^1* 7true Catharanthus roseus(Apocynaceae)is a subshrub plant with important medicinal and ornamental values. In the previous experimental planting, we found that C. roseus had good adaptability to the environment on tropical coral islands. In order to explore the ecological and physiological adaptabilities of C. roseus to tropical coral island environment, we investigated the morphological and anatomical structures, physiological characteristics, and nutrient element contents of C. roseus were analyzed by taking samples from a nursery in Wenchang City, Hainan Province and those transplanted to tropical coral island as study objects. The results were as follows:(1)C. roseus transplanted to tropical coral island had thicker leaves, more developed palisade tissue, and lower specific leaf area than that growing in nursery and other stress-tolerant plants, which were beneficial to its light energy absorption, water storage and resource utilization.(2)Compared with C. roseus grown in the nursery, C. roseus transplanted to tropical coral island had higher level of superoxide dismutase(SOD), peroxidase(POD)and catalase(CAT)activities, indicating a strong antioxidant enzyme activity and anti-stress ability of this species.(3)However, C. roseus transplanted to tropical coral island had low chlorophyll a and chlorophyll b contents, which could reduce excessive light energy into the chloroplast photosynthetic system and prevent excess light energy from harming the photosynthetic system.(4)Though the coral sand soil that C. roseus growing has a low nutrient content, the nutrient content in C. roseus leaves was high, indicating a good capacity in absorbing and utilizing nutrients of this species. Therefore, C. roseus had a good adaptability to arid and barren habitats, and could be used as a tool species for revegetation restoration on tropical coral islands.]]> 2020/4/13 0:00:00 1,2, LIU Nan2, WANG Jun2, REN Hai2, CAI Hongyue2, HUANG Yao2, LIU Nian1, JIAN Shuguang2*]]> TONG Shenghong1,2, LIU Nan2, WANG Jun2, REN Hai2, CAI Hongyue2, HUANG Yao2, LIU Nian1, JIAN Shuguang2* 6true Casuarina equisetifolia, Vitex trifolia var. simplicifolia, Spinifex littoreus in the coast area of Pingtan Island, Fujian Province, were selected and their NSC contents in leaf and stem were determined by an improved phenol-sulphuric acid method. The results were as follows:(1)Soluble sugar contents were the largest in both leaf and stem of Vitex trifolia var. simplicifolia,(137.83 ± 3.75)and(90.74 ± 2.65)mg·g^-1 respectively, while starch contents were the largest in both leaf and stem of Spinifex littoreus,(105.41 ± 2.49)and(120.48 ± 7.67)mg·g^-1, respectively, and NSC contents in above two species were all significantly larger than those in Casuarina equisetifolia(P<0.05).(2)The NSC and its components contents in leaf of Spinifex littoreus on the coppice dunes from rudimental period, developmental period to stabilized period showed no obvious trends, while decreasing trends were observed in its stem along the succession gradients, in which NSC and its component contents of S. littoreus on the coppice dunes in rudimental period were significantly larger than those in other two succession stages(P<0.05)and showed a decreasing trend.(3)From the young-aged, middle-aged to old-aged growth period, the NSC and its component contents in leaf and stem of Casuarina equisetifolia firstly demonstrated an increasing trend, then followed by a decreasing trend. The soluble sugar, starch and NSC contents in the leaf, soluble sugar and NSC in the stem of old-aged C. equisetifolia all suggested more significant lower contents than them in other two growth periods(P<0.05).(4)Species specific characteristics and its regeneration should be fully considered in the vegetation reestablishment in coastal sand dune ecosystems, due to the different adaptation strategies of species under various environmental stresses.]]> 2020/4/13 11:14:56 1, DU Jianhui^1,2*, ZHANG Qi¹, WANG Song¹, HUANG Chudan³, SHAO Jiayi¹]]> QIN Jing¹, DU Jianhui^1,2*, ZHANG Qi¹, WANG Song¹, HUANG Chudan³, SHAO Jiayi¹ 5true Casuarina equisetifolia coastal protection forest(C. CPF)in Hainan Island exists unable to naturally regenerate by itself. To explore obstacle factors of unable natural regeneration of C. equisetifolia itself, the ecological conditions affecting seed germination of C. equisetifolia, such as C. equisetifolia allelopathy, soil pH, salinity, temperature, matrix type and moisture were studied. The reason is that ecological conditions affecting seed germination are one of the three conditions that restrict the success of natural regeneration, and the other two conditions are the quantity and quality of provenances, and ecological conditions for survival of seedlings and saplings respectively. C. equisetifolia cones, litters and in-situ habitat soil were collected from the C. CPF near Houwei Village, Haikou City, Hainan Island. The seed germination test was conducted by paper dish method, with 100 seeds per dish and three repeated treatments. The C. equisetifolia litter, humus and sandy soil extracts were diluted into five concentration gradients respectively and a total of 15 extracts were obtained. The pH was set to 5.0, 5.5, 6.0 and 6.5 concentration gradients for the germination solution. Salinity was set to 0.02%, 0.05% and 0.10%. The temperature was set to 25, 30 and 35 ℃. The PEG method was used for water stress test, with five concentration gradients of 0, 50, 100, 150 and 200 g·L^-1. The orthogonal test was designed as three factors and four levels. The results were as follows:(1)There was no significant difference of seed germination rate between CK group and the one treated with different water extract concentrations of different extracts.(2)pH, salinity and temperature within the set range had no significant effects on seed germination rate of C. equisetifolia.(3)The germination rate of C. equisetifolia seeds treated with PEG solution with different concentration gradients was significantly different, and the seed germination rate of C. equisetifolia decreased sharply with the increase of PEG solution concentration.(4)Different matrices and watering frequencies also had significant effects on seed germination rate. Combining PEG drought stress, matrix and watering frequency, it can be found that C. equisetifolia seeds have weak drought resistance and are sensitive to water, therefore, moisture is the main limiting factor for the germination of C. equisetifolia seeds. Meanwhile, the poor water retention of litters and coastal sandy soil also restricts the germination of seeds to varying degrees.]]> 2020/4/13 11:14:56 *]]> WANG Yu, YANG Bin, HAO Qingyu^* 4true Casuarina equisetifolia coastal windbreaks, and to select the species with good natural regeneration under natural conditions, the natural regeneration status of Hainan C. equisetifolia coastal windbreaks was systematically investigated in this study by typical sampling method, and the natural regeneration characteristics of C. equisetifolia in different dry and wet climate zones were compared and analyzed by using the indexes of natural regeneration density and species richness; the tree species with better natural regeneration quality were screened by regeneration density and regeneration index. A total of 73 temporary quadrats were set, with the total sample areas of 2.51 hm². The results were as follows:(1)The overall quality of natural regeneration in C. equisetifolia costal windbreaks was not good, and C. equisetifolia could not achieve the natural regeneration by itself, but the natural regeneration of some other species was good in local area, and the percentage of good regeneration was 15.1%.(2)The natural regeneration richness and diversity in the wet zone were much better than that in the semiarid zone, in which there were 28 families, 50 genera and 59 regeneration tree species in the wet zone; there were only 6 families, 6 genera and 6 regeneration tree species in semiarid zone.(3)The regeneration of trees was better than that of shrubs.(4) Litsea glutinosa, Brucea javanica, Acacia confusa, Terminalia catappa, et al. were found to have stronger natural regeneration capability, therefore, they were suitable for mixing plantation with Casuarina equisetifolia and could be used as mixed tree species or associated tree species of C. equisetifolia costal windbreaks.]]> 2020/4/13 11:14:56 *]]> YANG Bin, WANG Yu, HAO Qingyu^* 3true Casuarina equisetifolia coastal protection forest(C. CPF)is an important barrier of coastal ecology in Hainan Island, and natural regeneration is of great significance for it to play a role of protection function continuously. It is found that undergrowth natural regeneration is difficult in the most area of C. equisetifolia forests in Hainan Island, however, there is a phenomenon of good regeneration in the local area. In order to explore the cause of the great disparity in the natural regeneration, and to provide certain theoretical basis for promoting the transformation of C. CPF from plantation to near-natural forest, the main factors affecting the natural regeneration of C. CPF were studied through the analysis of the differences of regeneration quality for different forest lands. Seventy-three temporary plots were established in the C. CPF in Hainan Island, and statistical methods, such as variance analysis and correlation analysis, were used to study the effects of climate zones, stand conditions, soil factors and litter accumulation on natural regeneration quality and density. The results were as follows:(1)The regeneration of C. CPF in wet zone was significantly better than that in dry zone;(2)There was significantly negative correlation between C. equisetifolia stand density and regeneration density or herb coverage, but other factors of stand conditions had little effect on regeneration;(3)There was no significant diffe-rences in soil pH value and nutrients in the sample plots with different regeneration qualities, but ammonium nitrogen promoted the regeneration of seedlings and organic matter did the regeneration of saplings to some extent;(4)The accumulation of litter was not conductive to natural regeneration as a whole. The results indicate that the climate factor, density of C. equisetifolia stands and accumulation quantity of C. equisetifolia litters were the main impact factors for the natural regeneration of undergrowth in C. CPF.]]> 2020/4/13 11:14:56 *]]> YANG Bin, WANG Yu, HAO Qingyu^* 2true 2020/4/13 0:00:00 *]]> ZHANG Zengke, WU Yahua, WANG Qi, JI Lingbo, HUANG Liujing^* 1true