Epicephala moths. At pollination stage, the floral scent could guide obligate pollinators to encounter their host plants for pollination service and laying eggs. Until post-pollination, it also plays other potential ecological functions. Here, floral volatiles of female flowers of Breynia fruticosa(Phyllanthaceae)at pre-pollination, pollination and post-pollination stages were collected by dynamic headspace absorption technique, respectively, then chemical components using gas chromatography-mass spectrometry(GC-MS). We have determined whether there are variations of floral scent at pre-pollination, pollination and post-pollination stages by conducting principal component analysis. The results showed that a total of 22 volatile compounds were detected from the floral scent of female flowers of B. fruticosa at pre-pollination stage(12 compounds), pollination stage(15 compounds), and post-pollination stage(13 compounds), which were mainly contained fatty acid derivatives and terpenoids. 3-Hexenal,(E)-2-Hexenal,(Z)-3-Hexen-1-ol,(Z)-3-Hexenyl acetate,(E)-β-Ocimene, β-Caryophyllene, β-Humulene,(E,E)-α-Farnesene and Longiborneol were considered as the major scent component(the relative amount>5% ). Multivariate statistical analysis showed there were obviously qualitative differences in chemical compositions of the floral scents from female flowers at pre-pollination, pollination and post-pollination stages, and such differences were result from changes of the main scents compounds among different flowering stages. For example, the content of(E)-β-Ocimene is the highest at pollination stage, whereas it significantly decreased after pollination. 3-Hexenal,(E)-2-Hexenal,(Z)-3-Hexen-1-ol and(Z)-3-Hexenyl acetate are the four most abundant compounds at pre-pollination stage. β-Caryophyllene obviously increased and Longiborneol newly appeared at post-pollination stage. Both β-Humulene and(E,E)-α-Farnesene sigificantly increased at pollination and post-pollination stages. In addition, the volatile release amount of female flowers at pollination stage emitted significantly more volatiles than that at pre- and post-pollination stages, respectively. The strong reduced emission of floral bouquet and significant variation in the chemical composition in female flower scents at post-pollination stage may be a function as post-pollination mechanisms to efficiently reduce further flowers visit, thereby to limit seed predation. It was specultated that such qualitative and quantity differentiation in floral scent from Phyllantheae plants at pre-pollination, pollination and post-pollination stages might have the important chemical ecological significance for maintaining in stability of nursery pollination mutualisms between Phyllantheae species and Epicephala moths.]]> 2020/8/17 12:39:59 1,2, WANG Zhibo², YANG Lifang³, SHI Fuchen^2*, LI Houhun²]]> HUANG Daihong^1,2, WANG Zhibo², YANG Lifang³, SHI Fuchen^2*, LI Houhun² 8true Cinnamomum camphora leaves released 78 types of VOCs during the whole year, and the types of terpenes(19)and alkanes(18)were more abundant than those of other compounds; The relative contents of 32 types of VOCs had significant differences(P<0.05)among different seasons; The number of types in March was the most abundant, and the relative contents of terpenes reached 43.49%, including 1-Caryophyllene, D-Limonene and α-pinene, and the types of VOCs released from C. camphora leaves in other months were mainly of alkanes and alcohols; In spring, the relative contents of VOCs released at most of the time were all mainly of terpenes, and the relative contents of terpenes released at 8:00 am were the highest. Therefore, C. camphora is an ideal species for ecological and heathy landscape, and the VOCs released from C. camphora leaves are rich in kinds of terpenes compounds, which are beneficial for human health, and spring and morning are the best time for forest recreation.]]> 2020/8/17 12:39:59 1, WANG Jinfeng¹, XU Yongqin², XIA Shufang¹, SHEN Fengqiang², XU Luyu², CHEN Zhuomei^1*]]> ZHOU Qi¹, WANG Jinfeng¹, XU Yongqin², XIA Shufang¹, SHEN Fengqiang², XU Luyu², CHEN Zhuomei^1* 7true Rhododendron fortunei in different flowering stages by headspace solid phase microextraction-gas chromatograhymass spectrometry(GC-MS)as well as principal component analysis(PCA). A total of fifty volatile components were detected throughout the whole flowering stages, which were mainly phenylproanoids/benzenoids, terpenoids, alcohols, aldehydes, hydrocarbons and others. The principal component analysis of twenty-nine major volatile components was carried out, and the cumulative bution rate of the two principal components was 88.545%. β-myrcene, β-ocimene, copaene, isoledene, eucalyptol, ylangene, epi-bicyclosesquiphellandrene, cyclohexene, 4-ethenyl-4- methyl-3-(1-methylethenyl)-1-(1methylethyl)-,(3R-trans)- were highly positively correlated with PC1. The contribution rate of the second principal component is 31.455%, and the effect of Eugenol is the largest and highly negatively correlated. These components are the key aroma components of Rhododendron fortunei flowers. Of the nine highly related components, terpenes accounted for seven species. Therefore, the terpenoids were the major aromas constituents for Rhododendron fortunei.]]> 2020/8/17 12:39:59 *]]> ZHANG Chenfei, XIE Xiaohong, WANG Qinghao, WANG Wenjing, WANG Jinyang, XIE Yu, WU Yueyan^* 6true 2020/8/17 12:39:59 1,2, CHEN Chuanwu^1,2, DENG Chongling^1,2*, LIU Ping^1,2, NIU Ying^1,2, TANG Yan¹]]> FAN Qijun^1,2, CHEN Chuanwu^1,2, DENG Chongling^1,2*, LIU Ping^1,2, NIU Ying^1,2, TANG Yan¹ 5true P<0.05). Studies have shown that there was a very significant positive correlation between the IV value, moisture content with kernel(P<0.01), in addition, there was a very significant positive correlation between the AV, POV, LOX and the MDA(P<0.01); Some of the indexes were(extremely)negatively correlated with each other; The result of principal component analysis showed that the moisture content and AV, POV, IV, MDA, CAT, LOX were closely related to the quality of the fruit, and it could be used as an important index to evaluate the degree of oxidation. The walnut with husk could be stored for 18 days at 1-2 ℃ and RH 85%-90%, after walnut with husk was picked and precooled. This research will provide theoretical basis and basic data for walnut with husk during cold storage.]]> 2020/8/17 12:39:59 WANG Jihui 4true 2^-), soluble sugar and antioxidant enzymes [superoxide dismutase(SOD), peroxidase(POD), catalase(CAT)and ascorbic acid peroxidase(APX)] during cold stress were detected and analyzed. The cold tolerances of the two varieties were evaluated comprehensively by membership function method. The results were as follows:(1)The rate of O₂^- production, MDA content and soluble sugar content increased with the increase of low temperature stress time, and the contents of MDA and soluble sugar in the process of low temperature stress were significantly different from those in the control group. The content of MDA and O₂^- production rate of 9311 were higher than those of DC907 during low temperature stress, and there were significant differences in MDA contents between the two cultivars at the late stage of low temperature stress. The soluble sugar content of DC907 was significantly higher than that of 9311 at the late stage of low temperature stress.(2)During low temperature stress, the activities of antioxidant enzymes(CAT, POD, SOD and APX)of DC907 were higher than 9311, and the activities of CAT and POD increased with the increase of cold stress time. SOD and APX activities decreased first and then increased with the increase of cold stress time. The results of physiological indexes showed that the cold tolerance of varieties was different. Among them, soluble sugar content, SOD, POD, CAT and APX activities were positively correlated with plant cold tolerance; and the accumulation of MDA and O₂^- was negatively correlated with plant cold tolerance.(3)The order of membership function values of these physiological indicators is consistent with that of average membership function values, which indicates that these indicators are closely related to cold tolerance of rice. According to the result of comprehensive evaluation of membership function value, the cold resistance of DC907 is better than 9311, which is consistent with the field observation results.]]> 2021/6/4 11:38:28 *]]> ZENG Zhichi, ZHANG Sichen, SHI Xiaocui, CHEN Baoshan, LI Wenlan^* 3true Chenopodium quinoa varieties in two different regions, the seeds of two C. quinoa varieties ‘LD-13'(low salt area)from Ledu District, Haidong City, Qinghai Province and ‘WL-192'(high salt area)from Wulan County, Haixi Prefecture, Qinghai Province were treated with different concentrations of NaCl solution to study the changes of seed germination indexes(germination rate, germination potential, germination index), growth indexes(fresh weight, root length, stem length)and physiological indexes(MDA content, SOD, POD, CAT activities). The results were as follows: Under the stress of low salt concentration(NaCl concentration less than 250 mmol·L^-1), the seed germination, seedling growth and physiological activities of the two C. quinoa varieties were suitable. However, under the high salt concentrations(NaCl concentration > 250 mmol·L^-1)stress, the seed germination, growth and seedling physiological activity were inhibited to varying degrees. According to the comprehensive evaluation value of salt tolerance, although the same valley type of C. quinoa varieties, ‘WL-192' variety adapted to saline-alkali soil were more salt-tolerant than ‘LD-13' variety which grown in low-salt soil. It is speculated that the salt tolerance of ‘WL-192' than ‘LD-13' may be affected not only by soil salinity, but also by varieties themselves, photoperiod, temperature, altitude, latitude and other external growth environment factors. Combined with the climatic environment and the development and utilization of saline-alkali land resources in the western region of Qinghai, ‘WL-192' variety are more suitable for popularization and cultivation in Qinghai.]]> 2021/6/4 11:38:28 1,2, YUAN Feimin³, LI Xiang^1,2, LIU Demei¹, CHEN Zhiguo^1*]]> QUAN Youjuan^1,2, YUAN Feimin³, LI Xiang^1,2, LIU Demei¹, CHEN Zhiguo^1* 2true Typha angustifolia and the contents of typhaneoside, isorhamnetin-3-O-neohesperidoside and isorhamnetin in different parts of growth periods. UPLC-Q-TOF-MS was used for qualitative analysis, combined with HPLC to analyze and evaluate the contents of flavonoids in different parts of T. angustifolia. A RPAZIS C₁₈ column(250 mm × 4.6 mm, 5 μm)was adopted. The mobile phase was composed of acetonitrile-0.05% phosphoric acid with a gradient elution. The flow rate was 1.0 mL·min^-1, the detection wavelength was 254 nm, and the column temperature was 30 ℃. The results were as follows:(1)A total of 42 compounds were identified in pollen, stem and leaf, and eight compounds with great differences in different parts.(2)The contents of three flavonoids in different parts of T. angustifolia during different growth periods in the same growth environment were significantly different.(3)The contents of isorhamnetin-3-O-neohesperidoside and isorhamnetin in the plant were increased first and then decreased, while decreased significantly in the stem and leaf; In pollen, the contents of typhaneoside and isorhamnetin-3-O-neohesperidoside increased quickly and reached a peak within a week; The contents of isorhamnetin in stem and leaf were low and were not detected in most samples. This established method in this study was simple and reproducible. The changes regularity of the flavonoids content in T. angustifolia had been studied and the differences of compounds in pollen, stem and leaf were also analyzed. This research provides a scientific basis for reasonable resource utilization of T. angustifolia.]]> 2021/6/4 11:38:28 1,2, GAO Mingliang², CAO Yudan², CHEN Peidong², YAN Hui^2*]]> CHAO Lei^1,2, GAO Mingliang², CAO Yudan², CHEN Peidong², YAN Hui^2* 1true