2020/10/16 14:33:36 1, LU Zhaocen¹, XU Weibin¹, YUAN Quan^{1, 2}, CHEN Hailing¹, LIU Yan^1*]]> LIN Chunrui¹, LU Zhaocen¹, XU Weibin¹, YUAN Quan^{1, 2}, CHEN Hailing¹, LIU Yan^1* 17true Cardamine tangutorum, Aralia chinensis, Cirsium shansiense and Smilax stans, which were favorite food of the Yi People in Liangshan, Sichuan Province. The results were as follows: There are at least 17 kinds of amino acids in these four wild vegetables, of which seven were essential amino acids, and a variety were medicinal amino acids. Among the four wild vegetable, the content of glutamic acid in Cirsium shansiense was higher, accounting for 0.41%, and the contents of aspartic acid in Smilax stans, Cardamine tangutorum and Cirsium shansiense were higher, accounting for 0.80%, 0.33%, and 0.05%, respectively. The amount of essential amino acid/total amino acid(EAA/TAA)and essential amino acid/non-essential amino acid(EAA/NEAA)of Cirsium shansiense were the highest, which were 34.44% and 67.78%, respectively. Compared with the cultivation of root vegetables and leafy vegetables on the Chinese Food Ingredients Tables, the protein content of Cardamine tangutorum was higher, accounting for 3.26%, and rich in vitamins(VA, VB, VB2, VC, VE and β-carotene). The custom of Liangshan Yi People like the four wild vegetables is closely related to their nutritional needs, dietary habits and culture. The results indicate that the four wild vegetables have the characteristics with high energy, low sodium, low crude fiber content, good taste and rich in mineral elements(copper, iron, zinc, manganese and magnesium), and have high nutritional value and health benefits. This study provides theoretical basis for scientific development and utilization of wild vegetable resources in Liangshan, and provides reference for the development of agricultural diversity in mountain areas.]]> 2020/10/16 14:33:36 1, FEI Yao^2*, ZENG Yonggang¹, YANG Qiliang³]]> WANG Jing¹, FEI Yao^2*, ZENG Yonggang¹, YANG Qiliang³ 16true Aristolochia delavayi populations, solid-phase microextraction followed by gas chromatography coupled with mass spectrometry detection was used to analyze the chemical composition of these plants. The relative amount of each compound was determined by area normalization method. The results showed that the main volatile component of dry leaves was(E)-2-Decenal, which accounted for 63.5%, 79.3%, 69.9%, 79.6% of the total volatile components, detected in the four populations of Shangri-La, Lijiang, Chuxiong, Luquan, respectively. However, the samples belonging to the Huangping population, Heqing County, showed a different volatile component pattern. The main volatile component from this population was bornyl acetate(30.1%)and(E)-2-Decenal only accounted for 4.5%, which was significantly lower than that of in the samples of the other four populations, and the possible reasons for the difference were analyzed and discussed. By analyzing the content of volatiles components in the leaves of A. delavayi, we identified the excellent germplasm of A. delavayi. At the same time, the healthy and safe eating methods were put forward according to the situation of aristolochic acid contained in the plant. The study also provides technical guidance and theoretical support for rational use of this traditional medicinal plant species and protection this endangered species in China]]> 2020/10/16 14:33:36 1, GENG Yupeng¹, CHANG Na¹, CHEN Gao^{2, 3*}]]> YU Yulong¹, GENG Yupeng¹, CHANG Na¹, CHEN Gao^{2, 3*} 15true Siraitia grosvenorii is an economically important plant due to the edible and medicinal value in Guangxi of China. However, severe pollination limitation has been found in the cultivated variety individuals of this plant, and consequently leads to a sharp rise of cultivation cost because of the highly depending on hand pollination. In order to explore the mechanisms of pollination limitation existed in S. grosvenorii, we compared the community of foraging insects and also the leaf volatiles of the male and female individuals of S. grosvenorii. Field investigations were conducted to observe the foraging insects of male and female S. grosvenorii. The volatile components of the leaves of male and female S. grosvenii were analyzed by headspace solid-phase-microextraction-GC/MS. The results were as follows: A total of 102 species of foraging insects were observed in male S. grosvenorii, belonging to 8 orders and to 29 families, including pollinators such as Apidae, Satyridae, Noctuidae and Sphingidae. There were 69 species of foraging insects observed in the female S. grosvenorii, which belonging to 7 orders and to 16 families, but the pollinators mentioned above were not found. The species richness, abundance and Shannon-Wiener diversity index of the foraging insects on the male S. grosvenorii were significantly higher than the female(P<0.05). The Jaccard's similarity index showed that the foraging insects on the female and male S. grosvenorii were at a medium dissimilarity level. Seventeen volatile components were identified from leaf of male S. grosvenorii, the main component are terpenes, accounting for 67.31% of the total content. And 12 volatile components were identified from the leaf of female S. grosvenorii, and the main components of which were alkane compounds, accounting for 44.27% of the total content. Male plants had many unique volatile components, including seven terpenes and three esters, accounting for 45.45% of the volatile components of S. grosvenorii. The female plants had fewer unique volatile components, including only four kinds of alkanes and one ester, accounting for 22.72% of the volatile components of the female of S. grosvenorii. Jaccard's index of similarity showed that the volatile components of female and male were generally moderately dissimilar, and the similarity of terpenes and esters was lower, which was extremely dissimilar. Further analysis indicates that the differences in the main components and the existence of unique components in leaf volatile components of male and female individuals may lead to significant differences in their foraging insects, and then affect the natural pollination of S. grosvenorii.]]> 2020/10/16 14:33:36 1,2, LU Qingbiao^1,2, HU Xinghua^2*, DENG Tao², DUAN Yunbo², FANG Zhenming^1,2, HUANG Shixun²]]> ZHU Xiaozhen^1,2, LU Qingbiao^1,2, HU Xinghua^2*, DENG Tao², DUAN Yunbo², FANG Zhenming^1,2, HUANG Shixun² 14true 2020/10/16 14:33:36 *, PEI Huimin, WEN Di, CHEN Zhi, LIU Rong, YAO Yuxian, Ma Yuan]]> WANG Fen^*, PEI Huimin, WEN Di, CHEN Zhi, LIU Rong, YAO Yuxian, Ma Yuan 13true Rehmannia glutinosa(RgC3H),C3H was mined from a candidate KEGG pathway based on the metabolomics analysis of R. glutinosa, and a conserved sequence of homologous gene was obtained in NCBI by multiple alignment. According to its fragment and SRA database of R. glutinosa, we cloned its full length CDS(RgC3H)by electro cloning and RT-PCR, and performed its bioinformatics analyses. The results were as follows: RgC3H gene was 1 530 bp in length and encoded a 509-amino acid protein with a molecular weight of 57.91 kD and without signal peptide; According to its amino acid sequence and structural analysis, RgC3H contained one conserved domain-cytochrome P450 domain,suggesting that it belonged to cytochrome P450 family; Phylogenetic tree analysis showed that RgC3H had high homology with the C3H genes of Sesamum indicum and Erythranthe guttata. This study laid a foundation for further research on the role of RgC3H gene in the pathway of verbascoside biosynthesis in Rehmannia glutinosa.]]> 2020/10/16 14:33:36 *, SHAO Luying, GUO Mengmeng, ZHU Jialin]]> ZHOU Yanqing^*, SHAO Luying, GUO Mengmeng, ZHU Jialin 12true Millettia speciosa. Based on the transcriptome sequencing results of non-enlarged and enlarged root of M. speciosa, 28 daphnia magna amylase genes were screened by bioinformatics technology. The results showed that the molecular weights of amino acid sequences encoded by 28 amylase-related protein genes ranged from 20.78 to 349.39 KDa. They were all acidic proteins, some of the subcellular localization was in chloroplast. They had PLN02784 super family and AmyAc-family super family conserved domains. The proportion of random coil in the secondary structure was the largest excluded from MsAm1, MsAm7, MsAm8, MsAm15, MsAm16, MsAm22, MsAm23 and MsAm28. Tertiary structure prediction showed that the amylase of M. speciosa contained α-amylase structure, β-amylase structure, and isoamylase structure. Amylase gene family had 86 functional elements, and MsAm9 had the most functional elements(42). The phylogenetic tree showed that MsAm15, MsAm16 belonged to the same category and had motif 2, motif 3, motif 7, and MsAm4, MsAm24, MsAm26 belonged to the other same category. Compared to Arabidopsis thaliana anylase, AtBM4 and MsAm6, AtAM2 and MsAm2, AtBM8 and MsAm5, AtBM4 and MsAm6, AtAM10 and MsAm22, AtIM3 and MsAm17 belonged to the same category, respectively. These results could provide a theoretical basis for the further study of biological functions and regulation mechanism of 28 M. speciosa amylase, and provide a reference for the study of root enlargement and improvement of M. speciosa amylase varieties.]]> 2020/10/16 14:33:36 1, 2,3, WANG Liuping¹, ZHAO Lichun ^1,2, HUANG Zhiqi¹, ZHAI Yongjin⁴, BAI Longhua⁴, MO Changming^5*]]> TU Dongping^{1, 2,3}, WANG Liuping¹, ZHAO Lichun ^1,2, HUANG Zhiqi¹, ZHAI Yongjin⁴, BAI Longhua⁴, MO Changming^5* 11true Epimedium myrianthum having much in common with E. sagittatum. There are transitions in many morphological characters and even in key taxonomy features. In order to explore the taxonomic relationship between the two taxa, the pollen morphology and ornamentation of exine of 13 populations of E. sagittatum(including 1 population of E. sagittatum var. glabratum T. S. Ying)and 7 populations of E. myrianthum were observed and compared by scanning electron microscope(SEM). The results showed that the pollen of both taxa was subspheroidal, with three-hole groove. Pollen size of E. sagittatum and E. myrianthum was(18.21-21.66)μm ×(19.55-23.63)μm and(18.32-21.43)μm ×(19.73-23.48)μm, respectively. The ornamentation of exine was an important character of pollen morphology. There were significant differences in ornamentation of exine among populations, which have been divided into three categories, namely reticulate, article reticulate and granular, and then divided into six subcategories according to the type of mesh ridge and mesh density. Among them, pollen morphology of E. sagittatum(including E. sagittatum var. glabratum)contained all three categories and six subcategories, and that of E. myrianthum contained three categories and four subcategories. This means that the pollen morphological variation pattern of E. myrianthum was a subset of E. sagittatum. On the whole, the variation pattern of the ornamentation of exine showed a trend, from reticulate to article reticulate, with the increase of latitude. And the main morphological characters of pollen were closely related to the elevation. As far as the pollen morphology concerned, the variation pattern of E. myrianthum was within the variation range of E. sagittatum, which was consistent with the variation patterns of other morphological characters. Pollen morphological characters could not distinguish the two taxa effectively, which failed to provide strong support for E. myrianthum as an independent species. It is suggested that the pollen morphological evidence is of relatively limited in clarifying the phylogenetic relationship among related taxa. However, further studies are still needed to provide strong evidence for the definition of large branches of phylogenetic trees of this genus.]]> 2020/10/16 14:33:36 *]]> HUANG Hua, LAI Huaqing, JIANG Yong, LI Renqing, LI Fengqin, LIU Yong, XU Yanqin^* 10true Meconopsis integrifolia in the late July of 2017 and 2018, we conducted two treatments, the shaded treatment and the petal removal treatment in the Balang Mountain population. A infrared temperature imager was used to monitor the daily dynamics of within-flower air temperature and the temperature of different floral organs of M. integrifolia; the ambient temperature was monitored by an environmental thermometer. The results were as follows:(1)Solar radiation significantly increased the within-flower air temperature and the flower temperature of M. integrifolia, and the heat of M. integrifolia flowers resulted mainly from solar radiation. The within-flower air temperature showed significantly lower difference between day and night than the ambient temperature, so M. integrifolia has a kind of floral temperature regulation function.(2)When the ambient temperature was high during the daytime, the solar radiation significantly increased the within-flower air temperature of M. integrifolia, and the petals reduced the within-flower air temperature; When the nighttime ambient temperature was low, the petals closure increased the within-flower air temperature, and the closed motion of the petals effectively reduce the within-flower air temperature difference between the day and night and produced a certain thermal insulation effect.(3)In the sun, the temperature difference of the floral organs was significant, the pistil and stamen temperatures were significantly higher than the petal temperature, and the temperature of the floral organs decreased from the center point of the pistil stigma to the outside. M. integrifolia could effectively regulate the temperature of various floral organs. M. integrifolia has a thermal regulation function, mainly through the petal closure to reduce the within-flower air temperature difference between the day and night and regulating temperatures of various floral organs in the sun. However, the temperature regulation mechanism is still unclear, and the adaptative significance needs further study.]]> 2020/10/16 14:33:36 *]]> ZHONG Tao, DUAN Xuyu, JIANG Yingying, LIU Guangli^* 9true Pennisetum sinese was used as the main research material, screening of growth-promoting strains from P. sinese, and explore the growth-promoting effects of growth-promoting strains. We used the following screening criteria for the determination of multiple growth-promoting capacities of primary strains: the ability to solubilize phosphorus, the ability to fix nitrogen, the ability to produce IAA. The strain YB-07 with the best growth-promoting effect was classified and identified through physiological and biochemical characteristics and 16S rDNA sequence homology analysis. Eleven strains with better overall performance were screened out and used for a pot experiment with a single inoculation and multi-microbe mixed inoculation to determine its growth-promoting effect. A total of 101 strains were isolated from the roots of the P. sinese, and the growth-promoting ability was measured. Among them, the strain with excellent overall performance was YB-07, which had a phosphorus content of 20.1 mg·L^-1, an IAA yield of 23.7 mg·L^-1, and an ability to produce ammonia at the same time. The results of pot experiment showed that the effect of multi-microbe mixed inoculation on wheat growth increased by 24.49%, 31.84%, 28.06% and 34.14% in the height, dry weight, fresh weight and chlorophyll content, respectively. Single bacterium inoculation increased the plant height, dry weight, fresh weight, and chlorophyll content by 13.54%, 20.45%,16.84% and 35.19%, respectively, compared with the control group. The selected strain has good growth-promoting effect, can provide a good seed resources for the further construction of the P. sinese flora promoting bacteria.]]> 2020/10/16 14:33:36 1*, CHEN Kaikai¹, LI Jing¹, LIU Xianchun¹, ZHANG Baobao¹, ZHANG Baocheng²]]> DENG Zhenshan^1*, CHEN Kaikai¹, LI Jing¹, LIU Xianchun¹, ZHANG Baobao¹, ZHANG Baocheng² 8true Stephania dielsiana, and the factors affecting the activity of chitosanase were preliminarily studied, in order to provide an experiment basis for further study on the enzymatic properties and the interaction between chitosanase-producing endophytic fungi and host plant resistance to diseases. Two endophytic fungi Stdif9 and Stdif9-4 were found to produce chitosanase by transparent circle method and liquid fermentation method. The highest activity of Stdif9-4(0.968 U·mL^-1)was significantly higher than that of Stdif9(0.780 U·mL^-1). Stdif9-4 was identified as Penicillium sp. Stdif9-4 by morphological method and sequencing analysis of ITS gene. The factors affecting on the chitosanase activity were studied by DNS reagent method. The results showed that the chitosanase activity reached maximum at 96 h of cultivation. In addition, among the nine metal ions, Mn²⁺ and Ca²⁺ had obvious activation effect on chitosanase activity. Ag⁺, Zn²⁺, Cd²⁺, Ba²⁺ and Fe³⁺ had different inhibitory effects on chitosanase activity, and Ag⁺ showed more significant inhibitory effect, while K⁺ and Na⁺ did not show significant effect on enzyme activity. There was no significant differences in enzyme activity between different cultured generations, which indicate that the enzyme activity is stable.]]> 2020/10/16 14:33:36 1,2,3, YAN Zhenling^1,2,3, HUANG Zhiqing^1,2,3, YUAN Haiying^1,2,3, LIU Guanghua^1,2,3, NONG Xiaoxia^1,2,3, LI Xin^1,2,3, LUO Haiyu^1,2,3*]]> JIN Qiuzhu^1,2,3, YAN Zhenling^1,2,3, HUANG Zhiqing^1,2,3, YUAN Haiying^1,2,3, LIU Guanghua^1,2,3, NONG Xiaoxia^1,2,3, LI Xin^1,2,3, LUO Haiyu^1,2,3* 7true Chaetomium globosum strain FS-01 against Alternaria panax, Sclerotinia schinseng, Botrytis cinerea, Rhizoctonia solani and Fusarium solani of ginseng pathogens, mycelia, fermentation broth and conidia suspension were tested in laboratory. The results showed that Chaetomium globosum FS-01 had certain inhibitory effects against five kinds of ginseng pathogens by confront culture. Among them, the highest inhibition rate to mycelia growth of Alternaria panax, reached 30.80%, followed by Rhizoctonia solani, Sclerotinia schinseng, Fusarium solani and Botrytis cinerea; On the PDA plate mixed with Chaetomium globosum FS-01 fermentation broth, the highest inhibition rate to mycelia growth of Botrytis cinerea, reached 82.09%, followed by Sclerotinia schinseng, Alternaria panax, Rhizoctonia solani and Fusarium solani; On the PDA plate mixed with Chaetomium globosum FS-01 conidia suspension, the highest inhibition rate to mycelia growth of Alternaria panax, reached 83.72%, followed by Botrytis cinerea, Rhizoctonia solani, Sclerotinia schinseng and Fusarium solani. Endophytic fungi FS-01 strain had a significant inhibitory effects, it could be utilized as a biological control resource inhibiting ginseng pathogens.]]> 2020/10/16 14:33:36 *]]> ZHOU Chunyuan, XU Shiquan, YAN Meixia, CUI Lili, HUA Shuang, WANG Yingping^* 6true Hippophae rhamnoides)oil is medically claimed as having phytoantioxidant, antiinflammatory, and anticancerous properties in humans. To explore the hydrogen peroxide of the sea buckthorn fruit oil on mouse monocyte macrophages(RAW264.7 cells)oxidative damage model. By spectrophotometry, scavenging effects of sea buckthorn fruit oil on DPPH free radicals; In vitro antioxidant activity was assessed on RAW264.7 cells against hydrogen peroxide(H₂O₂)induced cytotoxicity. The antioxidant effect was determined by measuring the cell dye MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reducing assay. Then the survival rate of RAW264.7 cells was determined by MTT assay, and the expression of superoxide anion was determined by flow cytometry labeling with dihydroethidium(DHE). The results were as follows:(1)Compared with the antioxidant capacity of vitamin C, when the concentration of sea buckthorn fruit oil was less than 4.9%, the antioxidant capacity of sea buckthorn fruit oil was higher than vitamin C.(2)Comparing to the H₂O₂ group, 3.125% sea buckthorn fruit oil group had higher survival rate(P<0.01).(3)Comparing to the H₂O₂ group, the proportion of DHE-positive cell was significantly decreased in the same time(P<0.01)and it was negative effect with concentration of sea buckthorn fruit oil, the proportion of DHE-positive cell was significantly increased at the same concentration(P<0.01), and which was related positive effect with concentration of sea buckthorn fruit oil(P<0.01). These results support the protective effects of sea buckthorn fruit oil against hydrogen peroxide-induced toxicity in vitro with the underlying mechanisms of inhibiting oxidative stress. Taken together, these results clearly indicate that sea buckthorn fruit oil has significant potential as a natural antioxidant agent, which could be a potential source for the discovery of natrual antioxidant health product.]]> 2020/10/16 14:33:36 1, WANG Bo^2*, WANG Qian¹]]> ZHENG Peng¹, WANG Bo^2*, WANG Qian¹ 5true in vitro. The diameter of the zone of inhibition was determined by agar diffusion method, and the minimum inhibitory concentration(MIC)and the minimum bactericidal/fungicidal concentration(MBC/MFC)were determined by micro-broth culture. The results were as follows: MIC/MBC values of 16 kinds of extracts Gentiana rigescens, Hypericum patulum and Rabdosia serra were 0.19 to 3.12 mg·mL^-1, which had very strong antibacterial activities to Staphylococcus accreus. The MIC/MBC values of 14 kinds of extracts Polygonum capitatum, Lophatherum gracile and Scutellaria barbata were 1.56 to 6.25 mg·mL^-1, which had good antibacterial activities to Pseudomonas aeruginosa. In addition to the Sophora japonica extract, the MIC/MBC values of other extracts were 3.12 to 12.5 mg·mL^-1, which had good antibacterial activities to Escherichia coli. The MIC/MFC values of Agastache rugosa and Daemonorops margaritae extracts were 0.78 to 6.25 mg·mL^-1, which had strong antibacterial activities to Candida albicans; The MIC/MFC value of Gentiana rigescens, Hypericum patulum, Geumja ponicum, Sophora flavescens, Piper nigrum, Penthorum chinense, Piper longum and Lophatherum gracile extracts were 6.25 to 12.5 mg·mL^-1, which also had certain antibacterial activities to Candida albicans. Therefore, these selected Chinese herbal medicines had good antibacterial effects, and most of them had broad-spectrum antibacterial activities, but Agastache rugosa and Daemonorops margaritae, which had strong antibacterial activity for Candida albicans, and other Chinese herbal medicines had strong antibacterial activity for Staphylococcus aureus, such as Hypericum patulum, Geumja ponicum, Agrimonia pilosa, Sophora flavescens, Penthorum chinense, Rabdosia serra. Above several kinds of Chinese herbal medicines can provide some reference for further reachering of its active monomer compounds and mechanism of action.]]> 2020/10/16 14:33:36 1,2, ZUO Guoying^1*, WU Yuxia^1,3, ZHANG Tiehuan^1,2]]> DIAN Zuohong^1,2, ZUO Guoying^1*, WU Yuxia^1,3, ZHANG Tiehuan^1,2 4true Dendrobium sinense was reported rarely about their chemical constituents and pharmacological activities. In order to comprehensively study the chemical constituents of this species, various column chromatography including MCI, silica gel and Sephadex LH-20 were conducted, through which ten compounds were isolated from the ethyl acetate fraction of ethanol extraction of the whole herb. On the basis of the physical data and spectral analysis, the structures were identified as matairesinol(1), 4,5-dihydroxy-2,3-dimethoxy-9,10-dihydrophenanthrene(2), dendrosinen C(3), 4-methoxy-phenylethanol(4), eis-p-hydroxyl ethyl cinnamate(5), p-hydroxyphenylpropionic ethyl ester(6), syringaldehyde(7), 3-hydroxybenzaldehyde(8), 3,9-dihydroxy-megastigma-5-ene(9)and(9Z,12Z)-methyl octadeca-9,12-dienoate(10). Compounds 1, 4-6, 8-10 were obtained from this plant for the first time. The bioactivity results indicated that compounds 2, 4 and 9 showed a certain acetylcholinesterase inhibitory activity.]]> 2020/10/16 14:33:36 1,3, TAN Caiyin^1,2, CHEN Huiqin^1,3, WANG Hao^1,3, MEI Wenli^1,3, SONG Xiqiang², DAI Haofu^1,3*]]> CAI Caihong^1,3, TAN Caiyin^1,2, CHEN Huiqin^1,3, WANG Hao^1,3, MEI Wenli^1,3, SONG Xiqiang², DAI Haofu^1,3* 3true Ainsliaea yunnanensis was isolated and purified by the normal pressure silica column chromatography, Sephadex LH-20, the low pressure cyano chromatography and preparative high pressure liquid chromatography. Ten compounds were isolated and identified as 3β-hydroxystigmast-5-en-7-one(1), 3β-hydroxystigmast-5,22-dien-7-one(2), β-sitosterol(3), stigmasterol(4), β-daucosterol(5), stigmasterol glucoside(6), 3,5-O-dicaffeoylquinic acid(7), 3,5-O-dicaffeoylquinic acid methyl ester(8), caffeic acid(9), protocatechuic acid(10). All compounds are isolated from this plant for the first time, and compounds 2 and 8 are isolated from this genus for the first time. The discovery of the compounds may enrich the chemical constituents of A. yunnanensis and provide a theoretical basis for its rational and effective application.]]> 2020/10/16 14:33:36 1,2, LI Jinjie³, ZHONG Xiangjian³, ZHOU Na³, WANG Xin³, LIN Pengcheng^1,2*]]> WU Lei^1,2, LI Jinjie³, ZHONG Xiangjian³, ZHOU Na³, WANG Xin³, LIN Pengcheng^1,2* 2true Kadsura coccinea leaves assisted by ultrasound extraction method, the ultrasonic-assisted extraction method of total flavonoids from K. coccinea leaves was studied, the influence factors of extraction time, ethanol concentration, extraction temperature and solid-liquid ratio on the extraction rate of total flavonoids from K. coccinea leaves were studied by single factor expe-riment, and orthogonal experiment was used to optimize the extraction process. The scavenging effects of total flavonoids extracted from K. coccinea leaves on DPPH, ·OH and superoxide anion were tested under the optimum conditions. The results showed that the optimal extraction conditions were as follows: extraction time 35 min, ethanol concentration 80%, extraction temperature 50 ℃ and solid-liquid ratio 1:20 g·mL^-1, the maximum yield was 4.83%. The antioxidant activity test showed that total flavonoids of K. coccinea leaves had strong scavenging abilities on DPPH free radicals, ·OH free radicals and superoxide anions. The order of antioxidant activities of total flavonoids from K. coccinea leaves was as follows: DPPH free radicals scavenging ability > superoxide anions scavenging ability > ·OH scavenging ability. At the concentration of 0.8 mg·mL^-1, the scavenging abilities of total flavonoids from K. coccinea leaves on DPPH, ·OH free radicals and superoxide anions were equivalent to 97.6%, 82.1% and 95.5% of Vc at the same concentration, respectively. Total flavonoids of K. coccinea leaves were good sources of natural antioxidants. This study provides theoretical information for the extraction and utilization of active ingredients from K. coccinea leaves.]]> 2020/10/16 14:33:36 1, Huang Guobao², QUAN Haiyan¹, YI Que¹, TAN Mingxiong^2*]]> LI Yajun¹, Huang Guobao², QUAN Haiyan¹, YI Que¹, TAN Mingxiong^2* 1true