Siegesbeckia glabrescens had significant physiological activity to inhibit necroptosis. In order to identify the active components and find the target compounds, the ethyl acetate extracts of S. glabresce were isolated and studied systemically in chemistry. The compounds were isolated and purified by chroma-tography on silica gel, ODS, and Sephadex LH-20 columns, and their structures were determined according to physicochemical properties and spectral analyses. Nine compounds were isolated from the ethyl acetate of S. glabresce and identified as 3,7-dimethoxyquencetin(1), apigenin(2), okanin(3), okanin-4'-O-β-D-6″-trans-p-coumaroylglucoside(4), 1H-indole-3-carbaldehyde(5), p-hydroxybenzaldehyde(6), 3,5-dimethoxy-4-hydroxybenzaldehyd(7), 3,4-divanillyltetrahydrofuran(8), buddlenol D(9). Compounds 2-5, 7-9 were isolated in this herbal plant for the first time.]]> 2021/1/7 0:00:00 1, JIN Yongliang¹, ZHANG Jiyue¹, ZHU Lingli¹, SHENG Tianlu¹, LIU Feng^2*]]> LIU Hua¹, JIN Yongliang¹, ZHANG Jiyue¹, ZHU Lingli¹, SHENG Tianlu¹, LIU Feng^2* 6true Amomum paratsao-ko, and the anti-inflammatory activity of total flavonoids from A. paratsao-ko was also investigated. The static adsorption-elution test screened out the HP-20 as the best macroporous adsorption resin for purifying the total flavonoids of A. paratsao-ko. The comprehensive scores of adsorption and desorption rates were used as the indexes to determine the optimal purification process parameters as follows: the concentration of the upper column sample solution 0.5 mg·mL^-1, the sample flow rate 4 mL·min^-1, and the upper column volume 15 BV, 70% ethanol 10 BV for elution, the elution flow rate 2 mL·min^-1. The obtained total flavonoids of A. paratsao-ko were evaluated for the anti-inflammatory activity via examination of the effect on IL-6 in LPS-stimulated BV2 microglial cells. The results showed that the total flavonoids of A. paratsao-ko significantly inhibit IL-6 expression in the LPS-stimulated BV2 microglial cells.]]> 2021/1/7 0:00:00 *]]> CHAI Ling, CHEN Mingsheng, YUAN Jiantong, FENG Jun, LIU Buming^* 5true in vitro against common clinical pathogenic bacteria. The dried powder of the collected 19 Chinese herbal medicine samples were extracted with 80% ethanol at room temperature and the solvent was evaporated under reduced pressure to get the ethanol extracts. In this study, each extract was screened for antimicrobial activity using the agar diffusion method, and minimum inhibitory concentration(MIC)and minimum bactericidal/fungicidal concentration(MBC/MFC)were determined by serial microdilution method. The results were as follows: Ethanol extracts of 19 Chinese herbal medicine had different degrees of antimicrobial activities. Inhibition zones of 14 ethanol extracts against SA, EC, PA and CA were between 8-27 mm, inhibition zones of the extracts from Euphorbia humifusa, Chloranthus japonicas, Berberis sargentiana, Thalictrum petaloideum and Rumex madaio were between 10.3-26.6 mm against SA and EC. The ethanol extracts from Thalictrum petaloideum, Cuminum cyminum, Euphorbia humifusa, Curcuma kwangsiensis, Andrographis paniculata, Leonurus artemisia, Evodia rutaecarpa, Rumex madaio, Helwingia japonica, Hymenodictyon flaccidum, Pteris multifida and Berberis sargentiana had significant antimicrobial activities against pathogens(MRSA and Pseudomonas aeruginosa resistant strain), the ranges of MICs/MBCs were between 391 and 6 250 μg·mL^-1; the lowest MIC values for Euphorbia humifusa and Berberis sargentiana against MRSA were obtained(respectively, 391 μg·mL^-1/781 μg·mL^-1), the lowest MIC value for Euphorbia humifusa and Berberis sargentiana was 1 562.5 μg·mL^-1against Pseudomonas aeruginosa resistant strain; the ethanol extracts from Thalictrum petaloideum, Cuminum cyminum, Berberis sargentiana were moderately inhibitory to the test Candida albicans resistant strain, and the sterilization effect was not obvious. These results provide the reference for selecting plant species as potential antibacterial compounds and antibacterial adjuvants of antibacterial and antifungal drugs.]]> 2021/1/7 0:00:00 1,2, WU Yuxia^1,3, DIAN Zuohong^1,2, ZUO Guoying^1*]]> ZHANG Tiehuan^1,2, WU Yuxia^1,3, DIAN Zuohong^1,2, ZUO Guoying^1* 4true accD gene was not differentially expressed between different tissues, indicating that the β-CT subunit may not be a limitation affecting factor for the function of acetyl-CoA carboxylase. The α-CT protein encoded by accD gene, with no signal peptide and was a hydrophobic protein. However, β-CT was a hydrophilic protein. KAS Ⅱ contained a conserved domain, and QcFb15, QcFb45 and QcFb75 were up-regulated in seeds, which were related to the chain extension of quinoa fatty acids and oil formation. The excavation of genes related to fatty acid biosynthesis pathway of quinoa will provide a theoretical basis for the study of the synthesis and accumulation of oil, and it has important enlightenment for the follow-up research of high oil quinoa varieties.]]> 2021/1/7 0:00:00 1,2, SUN Menghan^1,2, WU Qi^1,2, WU Xiaoyong^1,2, ZHAO Gang^1*]]> SHI Xiaodong^1,2, SUN Menghan^1,2, WU Qi^1,2, WU Xiaoyong^1,2, ZHAO Gang^1* 3true HcPR10(GenBank: KF673356)was isolated from the Suppression Subtractive Hybridization cDNA libraries of Halostachys caspica under the stress of 600 mmol·L^-1 NaCl. In order to investigate the biological function of HcPR10, the specific polyclonal antibody of HcPR10 was prepared by expression and purification of recombinant HcPR10 protein in vitro. In this study, recombinant prokaryotic expression vector pET28a-HcPR10 was constructed by double digestion and then was transformed into Escherichia coli strain BL21 to induce HcPR10 expression. We explored the optimal expression condition for soluble recombinant protein in BL21 by orthogonal analysis. Fusion proteins which were purified by the Ni-NTA affinity chromatography column were injected to BALB/c mice for preparing the HcPR10 polyclonal antibody. The titer and specificity of HcPR10 antiserum were detected respectively by ELISA and Western Blotting using recombinant protein His-HcPR10 and total protein of transgenic HcPR10 Arabidopsis thaliana. The results were as follows: The recombinant expression vector pET28a-HcPR10 was successfully constructed; The maximum amount of soluble fusion protein was obtained under the 27 ℃, 200 r·min^-1 and 0.7 mmol·L^-1 IPTG for induction 6 h; The antiserum for HcPR10 possessing 1:243 000 titer could bind specifically to recombinant protein His-HcPR10 and the heterologous protein from transgenic HcPR10 Arabidopsis thaliana. The high titer and specific antiserum for HcPR10 has been prepared successfully, the results of this study provide the foundation for further investigating the subcellular localization and biological function of HcPR10.]]> 2021/1/7 0:00:00 *]]> ZHANG Hua, YANG Tao, HENG Youqiang, WANG Yan^* 2true Hydrocotyle vulgaris on seed germination of other plants and to screen the main allelochemicals, the seed germination test was used to investigate the effects of leach liquors by different chemical solvents on seed germination, and the methods of gas chromatography mass spectrometry(GC-MS)and liquid chromatography mass spectrometry(LC-MS)were selected to analyse the compounds of water extracts of H. vulgaris and its rhizosphere soil which could affect other species. The results were as follows:(1)The different extracts of H. vulgaris inhibited the seed germinating of Raphanus sativus, Brassica chinensis, Cucumis sativus, and the inhibition effects were significant for Raphanus sativus.(2)By GC-MS, 35 and 17 compounds were identified from water extracts of plants and rhizospheric soils. The main compounds included dibutyl phthalate, 10,15-Octadecane diacid and 2,4-Di-tert-butylphenol in water extract of plants and oleic acid amide, N-hexadecane and ethyl palmitate in the water extract of rhizospheric soil.(3)By LC-MS, 109 and 93 compounds were identified from water extracts of plants and rhizospheric soils. By ESI⁺, L-phenylalanine and luteolin were the main compounds in water extract of plants and L-phenylbutyric acid and palmitamide were the main compounds in rhizospheric soil. By ESI^-, L-phenylpropyl dextro-quinic acid and chlorogenic acid were the main compounds in water extract of plants, and L-phenylpropanoid octadecanoic acid and sucrose were the main compounds in rhizospheric soils.(4)These compounds included butyric acid, octadecanoic acid, glycolic acid, oleic amide, palmitamide, ethyl hexadecanoate, benzoic acid could affect the growing and surving of companion organism directly, which belonged to fatty acids, amides, esters and aromatic acids. These compounds included oleic amide, palmitamide, ethy hexadecanoate, benzoic acid may originate in plants or transformed from secretion of plants by soil organisms. The input pathways of butyric acid, octadecanoic acid, glycolic acid include seereting of plants transforming of micro organisms and soil animals.]]> 2021/1/7 0:00:00 *]]> ZHOU Xu, HU Yaping, GE Xiaomin, CHEN Shuifei, MA Fangzhou, DING Hui^* 1true