RLPK2 gene(GenBank accession No. AY687391)is a receptor-like protein kinase gene that encodes a leucine-rich repeat(LRR)receptor kinase-type protein encoded with a N-terminal. In order to further analyze the function of the soybean RLPK2 gene, the overexpression vector of the RLPK2 gene was constructed via agrobacterium-mediated transformation of Arabidopsis thaliana. In this study, wild-type(WT)and transgenic A. thaliana plants were used as materials, and the variations in chlorophyll fluorescence parameters and the activities of antioxidant enzymes as well as the expression levels of senescence-associated genes in the aging process of leaves were investigated. The results were as follows:(1)Both WT and transgenic plants tended to decrease the efficiency of primary conversion of light energy of photosystem Ⅱ(PS Ⅱ)(F_v/F_m), actual photochemical efficiency of PS Ⅱ(Φ_{PS Ⅱ}), photochemical quenching coefficient(qP)and electron transport rate(ETR)while the latter showed a more obvious decreasing pattern as aging progressed.(2)The PS Ⅱ excitation pressure(estimated as 1-qP)was relatively stable in the early stage of leaf senescence, and increased sharply in the later stage of leaf senescence, while the transgenic plants showed a more obvious increasing trend.(3)At different leaf senescence stages, the malondialdehyde(MDA)content was significantly higher in transgenic plants than that in WT, while the activities of superoxide dismutase(SOD), peroxidase(POD)and catalase(CAT)were significantly lower in transgenic plants than those in WT.(4)Additionally, the real-time quantitative RT-PCR showed that the expression levels of aging marker gene ATSAG12, critical senescence-associated transcription factors ATNAP, ATWRKY6 and chlorophyll degradation key enzyme-encoding gene ATACD1 increased in transgenic plants. In summary, transgenic A. thaliana exhibited faster leaf senescence compared with WT, and the expression of the soybean receptor-like protein kinase RLPK2 gene played an important role in promoting leaf senescence.]]> 2022/6/10 16:05:09 *]]> ZHANG Qiang, HUANG Zhuoran, HU Kanglong, XU Chao, YANG Qingqing, XUE Tao^* 19true Lonicera fulvotomentosa has many functions including anti-inflammatory, antibacterial, immune regulation, etc., but it is easily confused with other species of Lonicera. The pharmacognostic research of L. fulvotomentosa will provide a scientific basis for its identification and the formulation of medicinal materials standards. This study combined botanical identification, microscopic observation, thin layer chromatography(TLC)identification, and molecular identification to identify specific characteristics of L. fulvotomentosa, from the aspects of botanical traits, microscopic characteristics of medicinal materials, TLC and ITS sequence feature. The results are as follows:(1)There are many secretory cells on the cross-section of the flower, many yellow-brown glandular hairs are on the surface of the bract has, the upper layer cell of corolla epidermis is polygonal, the pollen sac is mussel-shaped, the pollen grains are triangular or oval, and the oil chamber is oval.(2)The results of powder microscopy show that the middle column sheath fibers are short prisms; The cork cells are sharply angular and light yellow; The wood fibers are stubby and prismatic, a few of which are bent; There are many reticulated catheters, and the calcium oxalate square crystals are densely packed in the cell cavity.(3)TLC shows that the content of kaempferol in the flower of L. fulvotomentosa is higher, and kaempferol can be used as the identification component of L. fulvotomentosa.(4)The cluster analysis results based on ITS sequences show that the ITS sequence can be used as the DNA barcode to distinguish L. fulvotomentosa, L. macranthoides, L. confusa and L. japonica. This study provides a theoretical reference for the identification, component analysis, and quality standard formulation of L. fulvotomentosa.]]> 2022/6/10 16:05:10 1, 2*, XIAO Longqian^{1, 2}, YANG Yongping³]]> JIANG Xianghui^{1, 2*}, XIAO Longqian^{1, 2}, YANG Yongping³ 18true Pogostemon cablin medicinal materials, the pogostone-type one with higher content of pogostone is regarded the best. However, as a terpenoid component, the biosynthesis of pogostone is still not clear. MVA(cytoplasmic)pathway is an important pathway for terpenoid biosynthesis. In order to clarify the correlation between chemicals and related gene expression, in this study, the gene expression of HMGR, MK and MDD of cytoplasmic pathway and the chemical components in stems and leaves of two pogostone-type P. cablin cultivars(Shipai and Gaoyao)were studied, by using real time quantitative PCR to analyze gene expression and main components measurement. The results were as follows:(1)HMGR gene was more obviously expressed in the tender leaves of Shipai cultivar. The expression pattern of MK gene in Shipai cultivar and Gaoyao cultivar was similar, and MK gene was mainly expressed in old stems. MDD gene was more expressed in Shipai cultivar leaves than that of Gaoyao cultivar, but was expressed in similar patterns in the stems of Shipai cultivar and Gaoyao cultivar.(2)Both belonging to pogostone-type P. cablin, Shipai cultivar and Gaoyao cultivar had similar chemical components. Old leaves had the highest patchoulol content and old stems had the highest pogostone content.(3)MDD and MK genes were positively related to the synthesis of pogostone, which will lay a foundation for exploring key genes in P. cablin terpenoid metabolism pathway. In conclusion, the gene expression patterns of MVA pathway in two P. cablin cultivars are similar, and MDD and MK genes may be the key genes of terpenoid metabolism pathway in P. cablin.]]> 2022/6/10 16:05:10 1, HU Weiming², OUYANG Puyue^3,4*]]> KANG Dali¹, HU Weiming², OUYANG Puyue^3,4* 17true Pogostemon cablin cv. shipaiensis and P. cablin cv. zhanjiangensis with chemical component difference. The strains were classified by traditional morphological methods firstly, and then the rDNA-ITS sequences of the strains were amplified by the common fungal primer ITS1/ITS4 to identify their taxonomic status and their diversities. The results were as follows:(1)A total of 3 070 strains were isolated from the stem and leaf tissues of patchouli in seedling, branching and adult stages by PDA and LBA culture media. The 1 624 strains of endophytic fungi from P. cablin cv. shipaiensis were isolated and 1 319 strains were identified, which belonged to 36 genera. Seven endemic endophytic fungi were isolated from P. cablin cv. shipaiensis, including Epichloe typhina, Colletotrichum gloeosporioides, Botryosphaeria sp., Rhizoctonia sp., Truncatella sp., Phytophthora sp., and Sclerophthora sp., and the later two belonging to Oomycete were isolated for the first time. There were 1 446 strains isolated from P. cablin cv. zhanjiangensis, and 994 strains were identified, which were belonging to 33 genera. Among them, there were two endemic endophytic fungi in P. cablin cv. Zhangjiangensis, including Paecilomyces sp. and Cercospora sp.(2)The dominant endophytic fungi were Alternaria sp. and Colletotrichum sp. in two kinds of cultivated patchouli. The relative separation frequencies of the Alternaria sp. and Colletotrichum sp. were 9.48% and 7.81% in P. cablin cv. shipaiensis, and were 10.16% and 8.65% in P. cablin cv. zhanjiangensis.(3)The colonization rates of endophytic fungi gradually increased with the growth of patchouli from seedling to adult stages, which were Aug.(97.78%)> Jul.(72.50%)> May(55.28%)in P. cablin cv. shipaiensis, and Aug.(91.11%)> Jul.(63.06%)> May(46.67%)in P. cablin cv. zhanjiangensis. The average colonization rates were 75.19% and 66.95%.(4)There were a positive correlation between the growth periods and the Shannon index, which increased with the growth of patchouli. The average sorenson similarity index was 0.86. Obviously, P. cablin cv. shipaiensis and P. cablin cv. Zhangjiangensis had a large population of endophytic fungi and had their own endemic endophytic fungi, but the community structure was significantly different. These results lay a foundation for screening active strains and exploring the effect of endophytic fungi on the synthesis and accumulation of active components of patchouli.]]> 2022/6/10 16:05:10 1*, XIAO Jingjing¹ , DENG Yueting ¹, CUI Yexuan², LIU Zhifen¹]]> WANG Liguo^1*, XIAO Jingjing¹ , DENG Yueting ¹, CUI Yexuan², LIU Zhifen¹ 16true Melaleuca alternifolia by green deep eutectic solvents, four different extraction processes were compared including fresh-steam distillation extraction, dry-steam distillation extraction, freeze thawing-steam distillation extraction and deep eutectic solvents-distillation extraction. The effects of liquid-material ratio, distillation time, molar ratio of deep eutectic solvents, raw material drying time, water content on extraction rate of essential oil of M. alternifolia were investigated by single factor test. Based on single-factor tests, the quadratic regression parasitism model was established through the response surface methodology. The essential oil of M. alternifolia was determined by gas chromatography-mass spectrometry(GC-MS). The results were as follows:(1)Deep eutectic solvents-distillation extraction was the best extraction processes. The optimal extraction conditions were determined to be molar ratio of choline chlorid to 1,3-Butanediol 1:3(mol:mol), water content 52 mol, drying time of raw materials 9 h, material-liquid ratio 1:5 g·mL^-1, distillation time 60 min, and the maximum extraction rate of essential oil of M. alternifolia reached 4.06%, which was 383.33%, 290.38% and 497.06% higher than that of fresh-steam distillation extraction, dry-steam distillation extraction and freeze thawing-steam distillation extraction, respectively. Response surface methodology was proved to be applicable for extraction of essential oil from M. alternifolia, the experimental results were in good agreement with the predicted values of the response surface model.(2)The main components of essential oil of M. alternifolia extracted by four different extraction processes were proved to be almost same, while the contents were signifcanthy different.(3)Alcohols and alkenes were confirmed to be the main components in the essential oil of M. alternifolia. The extraction yield and characteristic component content of essential oil of M. alternifolia extracted by deep eutectic solvents-distillation were indicated much higher than those by other three processes,which including eight alcohols, nine alkenes and three other compounds, with the main components of γ-Terpinene,α-Terpinene,α-Pinene,α-Terpineol and alloaromadendrene, and the relative content of alkenes was 45.31%, the content of Terpinen-4-ol and eucalyptol was 30.58% and 0.12%. This study provides the data supporte for the further utilization of M. alternifolia and green extraction of natural products.]]> 2022/6/10 16:05:10 1, SHI Jinyi^1,2, WANG Donge¹]]> DU Hongxia¹, SHI Jinyi^1,2, WANG Donge¹ 15true Curcuma kwangsiensis in four harvest months(i. e. December 2018 and January, February, March 2019), gas chromatography-mass spectrometry(GC-MS)was used to analyze and identify the volatile components, and chemometrics method was used to analyze the volatile components with a relative content above 1%. The results were as follows: A total of 34 volatile components were identified by GC-MS analysis, and the relative contents of volatile components were different in different harvest months; The principal component cluster analysis was divided into four groups, and partial least square discriminant analysis showed that there were seven different compounds, among which the content of furanodienon reached the peak in January, and the relative contents of germacrone, humulene 2,3-epoxide reached the highest in February, while curzerene, terpinolene, isoborneol and epicurzerenone reached the peak in March. This study can provide a reference for quality control, growth process control and other development and research of Curcuma kwangsiensis.]]> 2022/6/10 16:05:10 1,2, HUANG Pan¹, XIE Xueting¹, WANG Qian¹, WANG Naibin¹, LU Yixiang^3*]]> ZHOU Gailian^1,2, HUANG Pan¹, XIE Xueting¹, WANG Qian¹, WANG Naibin¹, LU Yixiang^3* 14true Asclepias curassavica has been known to possess a broad range of biological activities, we sought to obtain the active endophytic fungi from this plant. We performed the study followed the idea of “ethnic drug-endophytic fungi-active ingredients”, and thereafter we examined the bioactivities of fungal metabolites from 168 strains of endophytic fungi isolated from A. curassavica. To evaluate the biological activities of anti-tumor, anti-inflammatory, anti-α-glucosidase and anti-oxidant in these fungi, the ethyl acetate extracts from fermentation broth of endophytic fungi were first determined using the methods of SRB, Griess, PNPG and DPPH, respectively. Furthermore, we identified the bioactive fungal strains with conserved ITS sequencing. The results were as follows:(1)A total of 22 in 168 isolated strains had variable biological activities. Nine strains had obvious anti-tumor effect with the IC₅₀ values of 0.1-40 μg·mL^-1. Further, the strain MJF-53 displayed prominent inhibitory effects on the release of both NO and IL-1β in Raw264.7 cell line by LPS- induction under the concentration of 2.5 μg·mL^-1. There were seven strains showing α-glucosidase inhibitory activity with the IC₅₀ values ranging from 1.0 to 4.0 mg·mL^-1, especially, the inhibitory activities of MYF-16 and MYF-55 were close to acarbose; 19 endophytic fungi had different degrees of DPPH free radical scavenging activities, among which MYF-9, MYF-19 and MJF-84 showed moderate anti-oxidant activities with IC₅₀ values of 13.562, 17.776 and 12.395 μg·mL^-1 respectively.(2)The ITS identification showed that the 22 active strains were mainly distribute in six genera: Alternaria sp., Colletotrichum sp., Fusarium sp., Diaporthe sp., Talaromyces sp. and Neofusicoccum sp. This study indicates that the bioactivities of the endophytic fungi from A. curassavica are diverse, which lays a foundation for exploring new potential natural anti-tumor, anti-inflammatory, hypoglycemic and anti-oxidant compounds from the endophytic fungi of A. curassavica.]]> 2022/6/10 16:05:10 1, SONG Nali², PU Xiaojia¹, GU Qianlan¹, YANG Haihao¹, WAN Chunping¹, QI Yan¹, LI Xiaosi¹, ZHENG Xi^1*]]> YIN Na¹, SONG Nali², PU Xiaojia¹, GU Qianlan¹, YANG Haihao¹, WAN Chunping¹, QI Yan¹, LI Xiaosi¹, ZHENG Xi^1* 13true Polygala fallax. Six compounds were isolated and purified by silica gel, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography(HPLC)from the ethyl acetate fraction of 95% ethanol extract of P. fallax. Combined with the 1D-NMR spectral data and literature comparison. The results were as follows:(1)Their structures were identified as kaempferol-3-glucoside(1), quercetin-3-O-β-D-galactoside(2), quercetin-3-O-β-xylopyranoside(3), quercetin-3-O-α-L-arabinoside(4), isorhamnetin-3-O-β-glucopyranoside(5), rutin(6), respectively. Compounds 1-6 were obtained from the plant for the first time.(2)The antioxidant activities of different polar parts and compounds were evaluated by using DPPH· and FRAP methods. The results showed that all compounds had antioxidant capacity, of which the total antioxidant activity of Compound 4 was up to(4.533±0.13)mmol·g^-1(vitamin C value was of 5.951±0.25 mmol·g^-1). Thus, the overall results not only enrich the material basis of flowers of P. fallax, but also provide scientific basis for its future development and utilization.]]> 2022/6/10 16:05:10 1,2, PAN Zhenghong^1,2, NING Desheng^2*, LI Lianchun², FU Yuxia², LI Haiyun¹]]> LI Gen^1,2, PAN Zhenghong^1,2, NING Desheng^2*, LI Lianchun², FU Yuxia², LI Haiyun¹ 12true Polygala fallax. The growth dynamics of spring shoots were monitored, and the contents of abscisic acid(ABA), auxin(IAA), gibberellin(GA), ethylene(ETH)and zeatin nucleoside(ZR)were determined by indirect enzyme-linked immunosorbent assay(ELISA)change. The results were as follows:(1)The growth and development of the spring shoots of P. fallax could be divided into rapid growth period(0-12 d), growth transition period(16-20 d)and slow growth period(24-32 d).(2)The contents of endogenous hormones ABA, GA, ETH and ZR in slow growth period were significantly higher than those in rapid growth period and growth transition period, and the difference of IAA contents in each period was small.(3)The spring shoot length, bottom leaf length and width were negatively correlated with ABA, GA, ETH and ZR contents in the rapid growth period, and were significantly correlated with ZR content, and positively correlated with IAA content; In the growth transition period, each index was positively correlated with GA, ETH and ZR contents, and was significantly correlated with GA content, but negatively correlated with ABA content; During the slow growth period, each index was positively correlated with the contents of five endogenous hormones, and was significantly correlated with the contents of IAA and ZR. The results provide a theoretical basis for the use of exogenous hormones to regulate the spring shoot extraction and growth of P. fallax.]]> 2022/6/10 16:05:10 1, TANG Hui^2,3, HUANG Xiyang^2,3, LIU Baoyu^2,3, LI Wenlan^1*]]> ZHANG Yuxian¹, TANG Hui^2,3, HUANG Xiyang^2,3, LIU Baoyu^2,3, LI Wenlan^1* 11true -1 and 15 g·L^-1, respectively. In addition, the results of liquid optimized culture showed that the hairy roots induced by NC82 and Va116 reached the maximum F(D)W at 25 g·L^-1 and 15 g·L^-1, respectively, which were 0.541 g(0.055 g)and 0.474 g(0.050 g)respectively, which was mutually corroborated with solid optimized culture.(4)The present data suggested that the concentration of sucrose in the medium significantly affected the growth of hairy roots. Considering the number of branches, F(D)W, and hairy root growth vigor, C58C1 induced NC82 hairy roots with optimal sucrose concentration was 25 g·L^-1, and the hairy roots of Va116 was 15 g·L^-1. The study of tobacco hairy roots optimized the suitable sucrose concentration in the medium, laying a foundation for subsequent large-scale propagation of hairy roots; at the same time, established a hairy root-AM fungus double culturing system and solved the problem of poor growth of hairy roots as a host.]]> 2022/6/10 16:05:10 *]]> XIANG Run, JIANG Long^* 10true Pedicularis kansuensis and Phtheirospermum japonicum, two hemiparasitic species from the family Orobanchaceae, were used as research objects. Agar culture experiments were conducted to investigate the effects of sucrose, DMBQ(2,6-dimethoxy-p-benzoquinone, an effective haustorium-inducing factor for root hemiparasitic Orobanchaceae species)and host plants on haustorium formation. The results were as follows:(1)Sucrose significantly promoted haustorium formation. Amendment with 2% sucrose increased the percentage of plants with haustoria in Pedicularis kansuensis and Phtheirospermum japonicum by 39.9% and 20.2% in the absence of host plants.(2)Sucrose enhanced haustorium-inducing activity of host plants. The number of haustoria per plant and ratio of haustoria with xylem bridges induced by host plants increased by 5.7 and 17.9% in Pedicularis kansuensis, and percentage of plants with haustoria and ratio of haustoria with xylem bridges increased by 76.7% and 16.2% in Phtheirospermum japonicum.(3)The promoting effect of sucrose on haustorium formation was of a similar magnitude as DMBQ for Phtheirospermum japonicum, with over 50% plants formed haustoria in both treatments.(4)Four percent was the optimal sucrose concentration for haustorium formation of two root hemiparasitic species, leading to the highest percentage of plants with haustoria(56% for Pedicularis kansuensis and 37.9% for Phtheirospermum japonicum). All the above results indicate that sucrose has significant promoting effects on both haustorium initiation and differentiation of the tested hemiparasitic species.]]> 2022/6/10 16:05:10 1,2, SUI Xiaolin¹, XUE Ruijuan¹, LI Yue^1,2, LUO Yan^1,2, LI Airong^1*]]> LI Yanmei^1,2, SUI Xiaolin¹, XUE Ruijuan¹, LI Yue^1,2, LUO Yan^1,2, LI Airong^1* 9true 18(4.6 mm×150 mm, 5 μm), and acetonitrile-glacial acetic acid(0.2%)solution(triethylamine adjusted pH to 6.20)was adopted as the mobile phase for gradient elution, with the flow rate of 1.0 mL·min^-1 and the detection wavelength of 235 nm. The fingerprints were analyzed by the software of “Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012)”. The discriminant analysis of hierarchical cluster analysis(HCA), principal component analysis(PCA), orthogonal partial least squares-discriminant analysis(OPLS-DA)and partial least squares-discriminant analysis(PLS-DA)were employed, combined with chemometrics and the determination results of principal components. The results were as follows:(1)HPLC fingerprints of 15 batches of Aconiti Radix and its processed products from three areas were established, 25 and 14 common fingerprint peaks were calibrated in 15 batches of raw and processed, six peaks were identified by the mixed reference product. They were benzoylmesaconitine(peak 16), benzoylaconitine(peak 17), benzoylhypaconitine(peak 18), mesaconitine(peak 21), hypaconitine(peak 22), aconitine(peak 23).(2)Chemometrics showed that 30 batches of samples were clustered into two categories, 15 batches of Aconiti Radix were classified into three classes, 15 batches of Aconiti Radix Preparata were classified into three classes. Six components containing mesaconitine and hypaconitine may be the potential different components of Aconiti Radix from different regions before and after processing.(3)The content determination results of principal components changed in varying degrees before and after processing, and the determination results of some principal components in Jiangyou of Sichuan producing area before and after processing were significantly higher than those in the other two producing areas. The fingerprint method which established in the study is accurate, repeatable, and reliable. Furthermore, combined with chemometrics and principal component content determination, it could be used for the quality control of Aconiti Radix and its processed product from different regions.]]> 2022/6/10 16:05:10 *]]> WU Dandan, LIU Yan, GUO Pengfei, KUANG Haixue, YANG Bingyou^* 8true Rosa laevigata, and to analyze the difference in the contents of gallic acid and catechin in roots of R. laevigata from different habitats and different processed methods resources from Guangxi. The contents of gallic acid and catechin of R. laevigata were used as evaluation index, the samples were determined by HPLC, and the data were analyzed by the analysis of variance(ANOVA)and discrimination study carried out based on the hierarchical clustering analysis(HCA)with SPSS 23.0 software. The results were as follows: The contents of gallic acid and catechin in roots of R. laevigata from different habitats and processed methods were different, the content of catechin was higer than that of gallic acid among all samples, the contents of gallic acid and catechin in roots of R. laevigata from the southern habitats(except for Guiping, Guigang)were generally higher than those from northern habitats, the contents of gallic acid and catechin were the highest in vinegar-processed products. The conclusion display that the differences of gallic acid and catechin contents in the roots of R. laevigata are mainly the differences of producting habitats and processed methods, the HPLC method is simple and feasible, which can provide a scientific basis for the resources development, quality control and clinical application of R. laevigata.]]> 2022/6/10 16:05:10 1, DENG Qi ¹, LU Xiaoling¹, SHU Ke^2*, ZHUO Shen¹, FAN Jiawen¹, PAN Sixing¹]]> WEI Xiyuan¹, DENG Qi ¹, LU Xiaoling¹, SHU Ke^2*, ZHUO Shen¹, FAN Jiawen¹, PAN Sixing¹ 7true Astragalus roots, Astragalus membranaceus and A. membranaceus var. mongholicus seedlings were put into seed germination bag with different concentrations of GR24 and IAA(2 μmol·L^-1 GR24, 5 μmol·L^-1 IAA and 2 μmol·L^-1 GR24+5 μmol·L^-1 IAA). Primary root length and lateral root number of Astragalus seedlings were measured, and endogenous phytohormones, the expression levels of auxin and strigolactone related genes were determined after seven days treatment. The results were as follows:(1)GR24 treatment could significantly promote the growth of primary roots of Astragalus.(2)The growth of primary roots was inhibited under IAA treatment, and the number of lateral roots was significantly increased.(3)The growth of primary roots under GR24+IAA treatment was also inhibited, the number of lateral roots of A. membranaceus was reduced compared with that under IAA treatment, indicating that GR24 can inhibit the induction of IAA to lateral root development, but can not alleviate the inhibition of IAA to primary root growth.(4)The levels of endogenous hormones, auxin and strigolactone related gene expression in the root of Astragalus seedlings under the three treatments were changed significantly, indicating that the effects of GR24 and IAA on primary root length and lateral root number of Astragalus seedlings may be related to these changes. The results preliminarily clarify the relationship between the root growth and development of Astragalus seedlings and GR24 and IAA, and provide a theoretical basis for the standardized breeding and seedling quality control of Astragalus. It also has certain significance to further explore the molecular mechanism of strigolactones and auxin regulating the growth and development of Astragalus root.]]> 2022/6/10 16:05:10 1,2,3, PANG Juan^1,2,3, CHEN Guilin^1,2,3*]]> HUANG Xiaoyu^1,2,3, PANG Juan^1,2,3, CHEN Guilin^1,2,3* 6true Pyrrosia lingua on α-glucosidase and α-amylase, their inhibitory activities of different polar extracts of 95% ethanol extracts of P. lingua were investigated by using p-nitrophenyl-α-D-glucopyranoside(pNPG)and 3,5-dinitro salicylic acid(DNS)methods with acarbose as positive control. Enzyme kinetics method and Lineweaver-Burk curve were used to analyze the types of inhibition of α-glucosidase for the most active extract. The results showed that the IC₅₀ values of the water extract were(4.71 ± 0.72)μg·mL^-1 for α-glucosidase and(48.40 ± 0.32)μg·mL^-1 for α-amylase respectively, which were significantly stronger than those of the other extracts(P < 0.05). The inhibition of α-glucosidase was stronger than that of acarbose, while the inhibition of α-amylase was weaker than that of acarbose. The IC₅₀ values of acarbose on α-glucosidase and α-amylase were(2 857.36 ± 1.35)μg·mL^-1 and(16.41 ± 0.63)μg·mL^-1, respectively. The enzymatic kinetics showed that the inhibitory type on α-glucosidase of water extract was reversible inhibition, while the Lineweaver-Burk curve showed a competitive inhibition. In conclusion, the water extract from P. lingua displays potential inhibitory activity on α-glucosidase and α-amylase, can be the natural sources of α-glucosidase inhibitor with less gastrointestinal side effects.]]> 2022/6/10 16:05:10 1,2, LING Meidi², WEI Aihong^1,2, LI Rongdi^1,2, LIU Xiaomin^1,2, NIE Hua^1,2, ZHANG Shengyuan^1,2*]]> ZHUANG Yuanbei^1,2, LING Meidi², WEI Aihong^1,2, LI Rongdi^1,2, LIU Xiaomin^1,2, NIE Hua^1,2, ZHANG Shengyuan^1,2* 5true p-hydroxycinnamic acid(8), o-hydroxycinnamic acid(9), o-methoxycinnamic acid(10), cinnamaldehyde(11), ferulic acid(12), ethyl caffeic acid(13). Among them, 1-5, 12 and 13 were isolated from the plant for the first time.]]> 2022/6/10 16:05:10 1, CHEN Guanyi², LIU Wenqin¹, WAN Pingnan¹, CHEN Zhongwen¹, LIU Hua^1*]]> JIN Yongliang¹, CHEN Guanyi², LIU Wenqin¹, WAN Pingnan¹, CHEN Zhongwen¹, LIU Hua^1* 4true Rubia oncotricha, the compounds were isolated with 70% ethyl alcohol and purified by silica gel, gel column chromatography, and their structures were elucidated by modern spectroscopic techniques. The results showed that fifteen anthraquinone compounds were isolated and identified from R. oncotricha, they were 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(6'-O-acetyl)-α-rhamnosyl(1→2)-β-glucoside(1), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-α-rhamnosyl(1→2)-β-glucoside(2), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(3'-O-acetyl)-α-rhamnosyl(1→2)-β-glucoside(3), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-β-glucoside(4), 1,3,6-trihydroxy-2-hydroxymethyl-9,10-anthraquinone-3-O-(6'-O-acetyl)-β-D-glucopyranoside(5), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(6'-O-acetyl)-β-D-glucopyranoside(6), physcion-8-O-β-D-glucopyranoside(7), emodin-8-O-β-D-glucopyranoside(8), digiferruginol-11-O-β-gentiobioside(9), 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(6'-O-acetyl)-β-D-xylopyranosyl-(1→2)-β-D-glucopyranoside(10), 6-hydroxyrubiadin(11), 1,2-dihydroxyanthraquinone(12), chrysophanol(13), 6-hydroxyxanthopurpurin(14), 1,3-dihydroxyanthraquinone(15). Compounds 7, 8 and 14 are isolated from the genus of Rubia for the first time, compound 1-6, 9, 10, 12, 13 are isolated from R. oncotricha for the first time.]]> 2022/6/10 16:05:10 1, 3, HUANG Hongyun^{1, 3}, HUANG Yong², HE Yanling^{1, 3}, WANG Yang^{1, 3}, LI Yongjun^1*]]> LI Yin^{1, 3}, HUANG Hongyun^{1, 3}, HUANG Yong², HE Yanling^{1, 3}, WANG Yang^{1, 3}, LI Yongjun^1* 3true 2022/6/10 16:05:10 1, YANG Lijuan², CUI Yasong³, CAI Qizong¹, XIA Yufei¹, CHEN Qingfu^1*]]> RAN Pan¹, YANG Lijuan², CUI Yasong³, CAI Qizong¹, XIA Yufei¹, CHEN Qingfu^1* 2true Lolium perenne)seedlings, a pot experiment was conducted by culturing perennial ryegrass in soil with treatments of GO in 0, 10, 20, 30, 40, 50 mg·g^-1 GO respectively. Parameters including plant growth, photosynthetic pigment contents, protective enzyme activity, malondialdehyde(MDA)content, leaf membrane permeability, soluble protein content as well as photosynthetic parameters were measured. The results were as follows:(1)No significant effects were detected on the growth of perennial ryegrass under 10, 20 mg·g^-1 GO treatments, however, 30-50 mg·g^-1 GO inhibited the perennial ryegrass growth. Under the 50 mg·g^-1 GO treatment, plant height and biomass of perennial ryegrass were the minimum in value, which were decreased by 16.8% and 27.1% compared with the control.(2)The contents of total chlorophyll and carotenoid decreased significantly when the concentration of GO reached 30 mg·g^-1, and the lowest value was found in 50 mg·g^-1 GO treatment.(3)High concentrations of GO(40, 50 mg·g^-1)decreased net photosynthetic rate(P_n), stomatal conductance(G_s)and transpiration rate(T_r), but increased the intercellular CO₂ concentration(C_i).(4)Low concentrations of GO(10, 20 mg·g^-1)had no significant effects on protective enzyme activity, MDA content, plasma membrane permeability and soluble protein content, while high concentrations of GO(40, 50 mg·g^-1)significantly increased superoxide dismutase(SOD), peroxidase(POD), catalase(CAT)activities, MDA content and plasma membrane permeability, decreased soluble protein content and damaged plant leaves. All the above results indicate that the addition of GO with high concentrations(40, 50 mg·g^-1)constitutes a stress on the growth of perennial ryegrass, thus inhibited plant growth.]]> 2022/6/10 16:22:02 *]]> HONG Ying, MAO Jianyue, ZHAO Shulan, DUO Li'an^* 1true