OsZAT12, a C2H2 zinc finger protein in rice, which cloned in our previous study, was only expressed in rice roots and was localized in the nucleus. Overexpressing OsZAT12 in Arabidopsis thaliana exhibited dwarf phenotype. To further investigate the function of OsZAT12 in rice, qRT-PCR was used to analyze the response patterns of OsZAT12 under abiotic stresses and phytohormones treatment. The results were as follows:(1)OsZAT12 contained two typical C2H2 zinc finger domains and one EAR motif, and has transcriptional repressive activity. The promoter of the OsZAT12 contained elements related to abiotic stresses and phytohormones.(2)The results of abiotic stresses and phytohormones treatment in rice also revealed that low temperature stress(4 ℃)and phytohormone abscisic acid(ABA)treatment significantly down-regulated OsZAT12 expression, while osmotic stress(20% PEG 6 000), phytohormone brassinosteroid(BR)or indole-3-acetic acid(IAA)treatment significantly up-regulated the expression of OsZAT12. These results showed that OsZAT12 involved in the changes in response to abiotic stresses and phytohormones in rice.(3)Homozygous OsZAT12 overexpression plants and OsZAT12 knockout plants were obtained using overexpression vector with 35S promoter and CRISPR/Cas9 gene editing technology, respectively.(4)Observation of the phenotype of OsZAT12 overexpression rice showed that compared with the wild type, the plant height of OsZAT12 overexpression plants was significantly shorter at tillering stage, heading stage and maturity stage. The plant height of OsZAT12 knockout plants did not change significantly compared with the wild type, while the panicle number and seed-setting rate of them were significantly lower than those of the wild type. These results indicated that OsZAT12 affected the establishment of agronomic traits such as rice plant type, panicle type and seed-setting rate.(5)The results in this study further showed that overexpression of OsZAT12 reduced the sensitivity of rice to exogenous ABA, while the opposite phenotype was observed in OsZAT12 knockout plants. Therefore, it is speculated that the effect of OsZAT12 on plant growth and development may be related to the regulation of this gene in response to abiotic stresses and hormonal signals, and this study provides an experimental basis of using OsZAT12 for molecular design breeding of stress-tolerant and stable yield in rice.]]> 2022/12/23 12:18:09 1, CHEN Zongxin¹, XIA Kuaifei², ZHANG Mingyong², WANG Yaqin^1*]]> CHEN Yanbo¹, CHEN Zongxin¹, XIA Kuaifei², ZHANG Mingyong², WANG Yaqin^1* 13true Oryza sativa formin homology 1(OsFH1)plays a crucial role in rice root-hair growth and development, and the short root-hair phenotype of osfh1 was changed by environmental factors. However, the mechanism of how environmental factors interact with OsFH1 to regulate rice root-hair phenotype is still unknown. To determine whether OsFHs function in the process of osfh1 mutant root-hair phenotype recovery. The expression of OsFHs was analyzed by qRT-PCR in the osfh1 mutant treated under 1/2 MS liquid culture and 1/2 MS solid culture. Furthermore, qRT-PCR results were compared with bioinformatics analysis results. The results were as follows:(1)When compared with the wild type, osfh1 primary root showed a no root-hair. The osfh1 mutant showed a shorter shoot and more lateral roots. However, the no root-hair phenotype of osfh1 recovered under 1/2 MS solid culture treatment.(2)The expression of OsFH16 decreased and the expression of OsFH17 increased in the osfh1 mutant from liquid culture treatment to solid culture treatment with signifcant differences.(3)OsFH1, OsFH16 and OsFH17 all belonged to the Type Ⅱ subfamily, and all had cis-acting elements related to environmental stress, such as auxin, gibberellin, and anaerobic. OsFH1, OsFH16, and OsFH17 may be located in the plasma membrane to perform functions.(4)Analysis of the tissue-specific expression pattern of OsFHs showed that OsFH1 was highly expressed in the roots, while OsFH16 and OsFH17 were lowly expressed in the roots. In conclusion, this study suggests that OsFH1, OsFH16, OsFH17 have conservative structures and similar regulatory modes, and all three may function on the cell plasma membrane, therefore, OsFH16, OsFH17 may be involved in the process that environmental factors and osfh1 together alter the root-hair phenotype. Overall, this study lays a theoretical foundation for the mechanism research of environmental factors and osfh1 gene co-regulation of rice root-hair development and proposes a new direction for exploring the function of plant formin genes.]]> 2022/12/23 12:18:09 1, LI Mingyu¹, DU Zhiye¹, WANG Kaishun¹, XIAO Kai¹, WANG Xin¹, SHI Yang¹, JI Hongli², CHEN Ji³,HUANG Jin^1*]]> LI Bin¹, LI Mingyu¹, DU Zhiye¹, WANG Kaishun¹, XIAO Kai¹, WANG Xin¹, SHI Yang¹, JI Hongli², CHEN Ji³,HUANG Jin^1* 12true OsMBF1c was cloned by PCR, the function of OsMBF1c was predicted by bioinformatics analysis, and the expression characteristics of OsMBF1c under Cd treatment was analyzed by RT-qPCR. The results were as follows:(1)The full length of OsMBF1c was 468 bp, which encoded 155 amino acids with the relative molecular weight of 16.154 kDa.(2)OsMBF1c was closely related to TdMBF1a.1, and cis-acting elements analysis showed that OsMBF1c was regulated by environmental factors such as light and anaerobic.(3)Gene expression analysis indicated that OsMBF1c was induced by Cd, and the expression level of OsMBF1c varied with different time or different tissues. After treated with 100 μmol·L^-1 Cd, the expression level of OsMBF1c in shoots at 1 h was remarkably up-regulated, which was seven times that of the control group, and the expression level in roots at 6 h was up-regulated to three times that of the control group. In conclusion, this study further refines the biological functions of MBF1 family under abiotic stress.]]> 2022/12/23 12:18:09 1, WANG Mengting¹, JIN Yufan¹, YU Yue¹, ZHANG Xu¹, LI Jiahao¹, JIANG Nan¹, LI Bin¹, CHEN Ji², HUANG Jin^1*]]> SHI Yang¹, WANG Mengting¹, JIN Yufan¹, YU Yue¹, ZHANG Xu¹, LI Jiahao¹, JIANG Nan¹, LI Bin¹, CHEN Ji², HUANG Jin^1* 11true s(20 676 bp).(2)There were 129 genes annotated in the chloroplast genome of Dalixiang, which could be divided into protein coding, tRNA and rRNA, with 85, 36 and 8 genes respectively.(3)Codon bias analysis of Dalixiang showed that leucine was most frequently used(1 944 times)and that of cysteine was used least frequently(198 times), and most codons ended in A/U(T).(4)The total number of SSR loci in the cpDNA of Dalixiang was 129, ninety-five of which were mononucleotide and most of SSR were composed of nucleobase A/T.(5)Phylogenetic analysis showed the closest affiliation relationship between Dalixiang and Tropical Japonica, and these two were clustered into one group. This study reveals the characteristic information of Dalixiang chloroplast genome, and identifies the phylogenetic status of Dalixiang.]]> 2022/12/23 12:18:09 *, LI Zujun, LONG Wuhua, GONG Yanlong, ZHU Susong]]> WU Chaoxin, LIU Xuewei^*, LI Zujun, LONG Wuhua, GONG Yanlong, ZHU Susong 10true Saccharum officinarum )responding to cold stress, the leaves of different sugarcane genotypes with different cold tolerance treated at 4 ℃ for 24 h were sampled as the materials for high-through transcriptome sequencing with Illumina HiSeqTM 2000, and 18 sRNA libraries before and after cold stress were constructed. The results were as follows:(1)A total of 322 known miRNAs of 84 families were discovered, and 110 new miRNAs were predicted. Among the known miRNAs, 100 differentially expressed miRNAs were screened out(61 up-regulated, 39 down-regulated), and 37 differentially expressed miRNAs(15 up-regulated, 22 down-regulated)were screened out from the new miRNAs.(2)A total of 1 844 target genes were predicted by using psRNATarget, TargetFinder and Tapirhybrid software. Three main functional categories of these target genes were revealed via the functional analysis of gene ontology, namely molecular function, cellular component and biological process.(3)In order to verify the reliability of high-throughput sequencing data, 14 miRNAs and their target genes were selected for qRT-PCR analysis, which showed that the 14 miRNAs were detected and most of the expressions were consistent with the sequencing results.(4)Some miRNA target genes were identified, which involved in plant growth, development and cold stress responses. All the above results indicate that miRNA in cold tolerant sugarcane directly or indirectly regulates the expression of target genes to realize the expression regulation of related metabolic pathways, and plays a key role in regulating the important agronomic traits.]]> 2022/12/23 12:18:09 *]]> ZHU Pengjin, SONG Qiqi, TAN Qinliang, CHENG Qin, LI Jiahui, PANG Xinhua, ZHOU Quanguang, LÜ Ping, OU Kewei, LU Yefei, NONG Zemei, TANG Huanwei, LONG Shengfeng^* 9true 2022/12/23 12:18:09 1,2, LUO Weigang³, HU Junming^2*, WEI Xianghua¹, HUANG Jiaqi^1,2, CHEN Shilin^1,2, MENG Yancheng², YU Yuefeng², LI Tingting², ZHANG Junhui², ZHOU Huirong², HUANG Zhonghua³, WEI Benhui⁴, CHEN Yuan⁴]]> HUANG Yuming^1,2, LUO Weigang³, HU Junming^2*, WEI Xianghua¹, HUANG Jiaqi^1,2, CHEN Shilin^1,2, MENG Yancheng², YU Yuefeng², LI Tingting², ZHANG Junhui², ZHOU Huirong², HUANG Zhonghua³, WEI Benhui⁴, CHEN Yuan⁴ 8true 2+, Mn²⁺, Zn²⁺ and Cd²⁺, plays an essential role in response to heavy metal stress in plant. To better understand the characteristics of the ScNRAMP gene family, bioinformatics methods were employed to identify and comprehensively analyze ScNRAMP gene family which includes protein physicochemical properties, gene structure, cis-acting elements, conserved motif, domain and evolutionary relationships. The results were as follows: A total of 29 ScNRAMP genes were identified in the Saccharum spontaneum genome; These 29 genes were unevenly distributed on 19 chromosomes, and contained 6 to 10 conserved motifs; The encoded proteins were all unstable proteins with no signal peptides and the subcellular locations were all on the plasma membrane; The number of membranes ranged from 6 to 12, and the secondary structure was composed of α-helix and random coils as the main components; Moreover, cis-acting elements analysis suggested that ScNRAMP may be involved in regulation of stress and development by involving in phytohormone metabolism; The tissue-specific analysis based on RNA-seq transcriptomics expression data of Saccharum spontaneum, showed that spatiotemporal expression of 29 ScNRAMP genes in the leaves and stems of sugarcane at different development stages; Phylogenetic analysis showed that the 29 ScNRAMP genes can be divided into three subfamilies(I, Ⅱ and Ⅲ). This study can be useful to better understand the ScNRAMP gene family and provide significant candidate genes that respond to the stress of heavy metals in sugarcane for further study.]]> 2022/12/23 12:18:09 1,2, YIN Ze^1,2, JIANG Yaolan^1,2, ZHOU Dinggang^1,2,3*]]> LIU Ying^1,2, YIN Ze^1,2, JIANG Yaolan^1,2, ZHOU Dinggang^1,2,3* 7true Saccharum officinarum. Twenty-two compounds were isolated and purified from the MeOH part of the stems and leaves of S. officinarum by means of various column chromatographic techniques, including SiO₂, Sephadex LH-20 and Rp-18 silica gel. Their structures were identified by mass spectrometry and nuclear magnetic resonance; The DPPH method was used to determine the free radical scavenging abilities of the components from S. officinarum. The results were as follows:(1)The compounds were identified as p-hydroxybenzaldehyde(1), p-methoxy-cinnamic acid(2), 4-methoxybenzaldehyde(3), vanillin(4), 4-hydroxy-cinnamic acid methylester(5), p-hydroxybenzoic acid(6),(2-Hydroxyphenyl)(phenyl)methanone(7), p-methylbenzoic acid(8), caffeic acid methyl ester(9), aconitate A(10), aconitate E(11), 5-O-dimethoxycinnamoylquinic acid(12), quercetin(13),quercetin-3-O-α-L-arabinoside(14), quercetin-3-O-β-D-galactopyranoside(15), didodecyl thiodipropionate(propionic acid, 3,3-sulfinyl di-1,1'-didodecyl ester)(16), α-conidendrin(17), rel-(2α,3β)-7-O-methylcedrusin(18), 3-O-Ferulylquinic acid methyl ester(19), luteolin(20),(5S,6S)-5,6-dihydro-3,8,10-trihydroxy-5-(4-hydroxy-3-methoxyphenyl)-6-hydroxymethyl-2,4-dimethoxy-7H-benzo [c]xanthen-7-one)(21), 5-O-Ferulylquinic acid methyl ester(22). Compounds 2-3, 7-11, 14-19, 21-22 were isolated from this plant for the first time.(2)Determination of free radical scavenging abilities of 15 compounds(1-9, 11-16)were selected by DPPH method. Compound 12(5-O-dimethoxycinnamoylquinic acid)had great antioxidant activity(IC₅₀ value was 49.58 μg·mL^-1). This study enrich the material basis of antioxidant activity of S. officinarum, which provides a scientific basis for the further development of S. officinarum.]]> 2022/12/23 12:18:09 *]]> LOU Hongbo, WANG Xianhong, HE Lilian, LI Fusheng^* 6true Saccharum officinarum is the main cash crop in sugar processing industry, and its leaves are characteristic Yao medicine in Guangxi with a long history. Our recent study showed that its ethyl acetate extract was partly responsible for its in vitro anti-tumor activity. In order to clarify the chemical constituents of this part, the modern separation and purification techniques, such as silica gel column chromatography, Sephadex LH-20 column chromatography, and semi-preparative high-performance liquid chromatography were used to identify the structures of the isolates by their physicochemical properties and modern spectral analysis. The results were as follows: Twenty compounds were all isolated and identified as 3, 4-dihydroxybenzaldehyde(1), methyl 3, 4-dihydroxy-benzoate(2), 3, 4-dihydroxy-benzoic acid(3), 3-hydroxy-4-methoxybenzoic acid(4), p-hydroxy-benzoic acid(5), p-hydroxybenzyl aldehyde(6), p-hydroxy-cinnamic acid(7), syringic acid(8), 3, 5-dihydroxy-hydroquinone(9),1-hydroxy-benzoyl-4-O-α-L-rhamnopyranoside(10), p-hydroxy-benzoyl-β-D-glucopyranoside(11), quercetin(12), tricin(13), tamarixetin(14),isorhamnetin(15), 5, 3', 4'-trihydroxy-7-methoxy-flavanone(16), 7-O-Methyleriodictyol(17), [(E)-4-(1S,3R,4R)-1-hydroxy-4,5,5-trimethyl-7-oxabicyclo [4.1.0]heptan-1-yl]but-1-en-3-o-ne(18), blumenol A(19)and thymidine(20), respectively. Compounds 1-4, 6, 9-11, 13-16, 18 and 20 were isolated and identified from this plant for the first time. The results provide some basis for its further development.]]> 2022/12/23 12:18:09 1,2,3, WEI Wei^1,2, HAO Erwei^1,2, XIE Jinling^1,2, DENG Jiagang^1,2, HOU Xiaotao^1,2,3*]]> XIE Anran^1,2,3, WEI Wei^1,2, HAO Erwei^1,2, XIE Jinling^1,2, DENG Jiagang^1,2, HOU Xiaotao^1,2,3* 5true 2022/12/23 12:18:09 *]]> ZHAO Ming, WU Peng, HE Haiwang, LONG Fang, MO Tianli, HUANG Xiang, ZOU Yu^* 4true Fusarium oxysporum f. sp. cubense is a soil-borne disease, which seriously threatens the sustainable development of banana industry. In order to seek an economic, effective and environmental protection measures, plate and pot experiments were carried out by using allelochemical strawberry acid(SA)to investigate the effects on hypha growth, disease severity index of banana wilt, soil microorganism quantity and soil enzyme activity. The results were as follows:(1)With the increase of SA concentration, the colony growth diameter of Foc4 decreased significantly, which decreased by 49.15% and 70.89% when SA concentrations were 300 μL·L^-1 and 450 μL·L^-1 compared with 150 μL·L^-1, respectively, on the fifth day. The number of spores were significantly lower than that of the control treatment(more than 470 times)when SA concentration was 600 μL·L^-1 under liquid medium condition. SA had a better inhibitory effect on Foc4 at pH 5 and was significantly better than that at pH 7 and pH 9.(2)As time going, the disease severity index of banana seedlings was significantly lower than that of the control after adding SA.(3)The numbers of soil bacteria, fungi and the total amount of microorganisms were all the highest when SA was 600 μL·L^-1; The number of Foc4 decreased with the increase of SA concentration, and significantly decreased when SA concentration was 1 200 μL·L^-1.(4)The soil enzyme activity was higher when SA concentration was 600 μL·L^-1,and was significantly decreased when SA concentration was 1 200 μL·L^-1, the activity of catalase and polyphenol oxidase were lower by 41.88% and 54.82% compared with the control, respectively.(5)Correlation analysis showed that the total amount of soil microorganisms was extremely significantly positively correlated with the numbers of bacteria and fungi. Soil fungi was significantly negatively correlated with actinomycetes. The numbers of soil bacteria, fungi and actinomycetes were all significantly positively correlated with invertase and polyphenol oxidase. Invertase and urease, catalase and polyphenol oxidase were all significantly positively correlated. In general, adding SA at a concentration of 600 μL·L^-1 can better inhibit the hypha growth of Foc4, increase its inhibition rate, and significantly reduce the disease severity index. Meanwhile, it can improve the growth environment of bananas. This study provides an academic reference for the effective use of SA to control banana wilt.]]> 2022/12/23 12:18:09 1,YANG Xiaodong², HE Chengxin¹, XU Guangping^1,3, HUANG Yuqing⁴, ZHANG Denan¹, SUN Yingjie¹, MOU Haifei⁵, WEI Shaolong⁵, ZHOU Longwu^1*]]> TENG Qiumei¹,YANG Xiaodong², HE Chengxin¹, XU Guangping^1,3, HUANG Yuqing⁴, ZHANG Denan¹, SUN Yingjie¹, MOU Haifei⁵, WEI Shaolong⁵, ZHOU Longwu^1* 3true -1 sodium selenate could significantly increase plant height of three banana varieties and the growth of basal stem circumference of ‘Nantianhuang' and ‘Hongxiangjiao', but not ‘Zhongjiao 9'.(2)The application of selenium had little effect on MDA content in leaves during vegetative growth, and MDA content increased or decreased significantly only in part time. The proline content in leaves of the three banana varieties were significantly reduced at 0.25 and 0.50 g·plant^-1 sodium selenate. Selenium had a significant effect on selenium content in leaves. The higher selenium concentration, the higher the selenium content in leaves.(3)The application of selenium could significantly increase the yield of banana and the single fruit weight of ‘Zhongjiao 9' and ‘Hongxiangjiao'. The yield per plant and single fruit weight of ‘Zhongjiao 9' at 0.25 g·plant^-1 sodium selenate were 24.38 kg and 165.86 g, which were 12.80% and 14.69% higher than those of the check(CK). The suitable concentration of sodium selenite could effectively increase the content of vitamin C and potassium in fruits.(4)The highest vitamin C contents of ‘Nantianhuang' ‘Zhongjiao 9' and ‘Hongxiangjiao' were 12.7, 13.9 and 10.6 mg·100g^-1, which were 12.72%, 18.84% and 29.39% higher than CK. And the highest contents of potassium were 349, 279 and 397 mg·100g^-1, which were 29.62%, 33.28% and 47.77% higher than CK.(5)The higher the concentration of selenium treatment, the higher the content of selenium in fruits. The selenium content of fruits in CK did not reach the standard of selenium enrichment. The three banana varieties reached the standard of selenium enrichment after treatment with 0.25 and 0.50 g·plant^-1 sodium selenate. In conclusion, the application of sodium selenate solution in soil can increase the content of selenium in banana fruits, promote the plant growth, improve fruit quality of banana, and decrease the content of MDA and proline in leaves. However, there are differences in the effects of various banana varieties. These findings will provide a theoretical basis for the production and cultivation of selenium enriched banana.]]> 2022/12/23 12:18:09 1, TIAN Qinglan¹, HUANG Weihua¹, WU Yanyan¹, PENG Jiayu², ZHANG Yingjun¹, XIE Rulin², WEI Shaolong³, MOU Haifei^1*, WEI Di¹]]> LIU Jieyun¹, TIAN Qinglan¹, HUANG Weihua¹, WU Yanyan¹, PENG Jiayu², ZHANG Yingjun¹, XIE Rulin², WEI Shaolong³, MOU Haifei^1*, WEI Di¹ 2true GA3ox gene from Williams B6 dwarfing mutant and its wild type parent were cloned by RT-PCR, and their presumed amino acid sequence were compared and analyzed. Meanwhile, the expression level of the GA3ox gene in different tissues of dwarf banana and its wild type were analyzed by qRT-PCR. The results were as follows:(1)The ORF lengths of the dwarf banana GA3ox-A and the wild type GA3ox-G were both 864 bp, and are all encoded with 287 amino acids. The comparison of the two amino acid sequences showed that there were five differences, which resulted in proteins with different properties.(2)Amino acid sequence homology analysis showed that the amino acid sequences of dwarf banana GA3ox had the highest homology with oil palm, date palm and coconut.(3)The qRT-PCR showed that the expression levels of GA3ox in dwarf banana leaves and stems were lower than those of wild type. The expression level of GA3ox in wild type stems was 2.2 to 32 times as high as that in dwarf plants. Therefore, these results illustrate that GA3ox gene may play an important role in regulating the dwarfing variation of banana stem.]]> 2022/12/23 12:18:09 *]]> LIN Jiaqi, LI Yanpei, XIAO Shixiang, FENG Dou, XUAN Weiyan^* 1true