Angiopteris esculenta is one of the most important wild substitute plant for staple food in Dulong's communities(the least populated ethnic group in Yunnan Province, China)for centuries. The Dulong people collect the underground parts of A. esculenta to extract flour and make it into food. In order to study and record the traditional knowledge of Angiopteris esculenta, ethnobotanical methods were employed to investigate and document the traditional knowledge about A. esculenta, and approaches of food science were used to reveal the basic components, morphology, physicochemical properties, and gelatinization characteristics of A. esculenta flour in the present paper. The results were as follows:(1) A. esculenta was a multipurpose plant in the traditional livelihood of Dulong people. Local people employed this plant for edible, medicinal, ornamental purpose and woody purposes. Quantitative analysis showed that the relative frequency of citation(RFC)of the edibleness was much higher than that of other uses, indicating that it was mainly used as staple food.(2)The traditional uses of fern flour extracted from the underground part of A. esculenta imply rationality. There was high starch content in the flour, and the total starch content was 86.2%, and A. esculenta flour contained various mineral elements necessary for human body.(3)The flour had good physicochemical properties, among which the retrogradation and freeze-thaw stability were strong, which could be used in the production and process of frozen food and starch products. The regeneration value and attenuation value of starch were much lower than that of common starch, which indicated that it had good stability of hot paste and cold paste, and not easy to regenerate, thus imply the good processability in food industry. In conclusion, A. esculenta flour has a good potential for utilization and further development. It is expected to play a positive role for increasing local residents' income and for rural revitalization in remote areas, through the approaches of artificial cultivation. The research results provide a theoretical basis for the subsequent processing and utilization of A. esculenta.]]> 2023/3/6 22:38:48 1,2, CHENG Zhuo^1,2, LONG Chunlin^1,2,3*]]> LU Xiaoping^1,2, CHENG Zhuo^1,2, LONG Chunlin^1,2,3* 23true in-situ or on-farm conservation of Changmaogu provides a great reference for the conservation of other landraces, and reveals the fact that the outstanding properties like high nutritional value of Changmaogu will contribute to the sustainable uses of Changmaogu and other landraces.]]> 2023/3/6 22:38:48 1,2, LONG Chunlin^1,2,3*]]> HE Liu^1,2, LONG Chunlin^1,2,3* 22true Zea mays, Broussonetia papyrifera, Ipomoea batatas, Morus alba, Fagopyrum dibotrys and Oryza sativa.(5)The local forage plants with high comprehensive evaluation were Broussonetia papyrifera, Ipomoea batatas, Morus alba, Fagopyrum dibotrys and Cucurbita moschata. The local people have rich traditional knowledge about the use and management of forage plants for pigs, which are very scientific and well preserved, and the related forage plant resources are also worthy of further development and promotion.]]> 2023/3/6 22:38:48 1, HU Qimin¹, NONG You¹, LUO Binsheng^2*]]> HU Renchuan¹, HU Qimin¹, NONG You¹, LUO Binsheng^2* 21true Dregea sinensis. Currently, the source plant has been corrected as Marsdenia tenacissima. In order to further clarify the source plants of “Dai-Bai-Jie”, we carried out an integrated analysis of the source plants of “Dai-Bai-Jie” and its related species in this study. Our key results were as follows:(1)Phylogenetic relationship of Marsdenia and other species of Marsdenieae using three DNA fragments(psbD-trnT, trnL-trnF, ITS)showed that all “Dai-Bai-Jie” samples were supported as monophyletic with the sample of M. tenacissima, and were sister to M. cavaleriei.(2)Comparative analyses of morphological characters with type specimen proved that morphological characters of the “Dai-Bai-Jie” samples were consistent with those of the type specimen of M. tenacissima and obviously different from those of M. cavaleriei. In this study, the source plant of “Dai-Bai-Jie” was identified as the species M. tenacissima. Meanwhile, it was easy to discriminate from M. cavaleriei in the leaf and floral characters. According to both morphological and molecular evidence, it has been confirmed that the source plant of “Dai-Bai-Jie” is M. tenacissima that can be used for rapid and accurate authentication of medicinal herbs in the market, and also benefit for the development and utilization of the “Dai-bai-jie” medicine in future.]]> 2023/3/6 22:38:48 1,2, TAN Yunhong^1,3, LI Haitao⁴, AI Handan⁵, GONG Yanxiong⁶, XIAO Yunxue⁷, YU Wenbin^1,3*]]> LI Jinyue^1,2, TAN Yunhong^1,3, LI Haitao⁴, AI Handan⁵, GONG Yanxiong⁶, XIAO Yunxue⁷, YU Wenbin^1,3* 20true Argyreia seguinii, 75% ethanol extract from A. seguinii was isolated and purified by silica gel, ODS, Sephadex LH-20 and semi-preparative HPLC, and the structures of obtained compounds were identified by physicochemical properties and spectral data. Five tumor cell cytotoxic activities of the compounds were determined by MTS method. Nine compounds were isolated and identified as trans-N-cinnamoyltyramine(1), trans-N-p-coumaroyltyramine(2), N-trans-feruloyltyramine(3), N-cis-feruloyltyramine(4), 1H-indole-3-carbaldehyde(5), aurantiamide acetate(6), 8-oxotetrahydropalmatine(7), 8-oxypalmatine(8)and 12,13-dihydro-8-oxoberberine(9). Compounds 1, 4-9 were isolated from this plant for the first time, and compounds 7 and 8 showed inhibitory activities against the cancer SMMC-7721 cells with the IC₅₀ values of(15.50 ± 0.76),(14.24 ± 0.72)μmol·L^-1, respectively. The results of this study lay a foundation for further study of the chemical constituents and pharmacological activities of A. seguinii, and provide a certain basis for clarifying the origin of Zhuang medicine “Yipichou”.]]> 2023/3/6 22:38:48 *]]> LIAO Guangfeng, LI Yunqing, MO Liuyan, LIU Cailan, LU Rumei^* 19true Bauhinia aurea was conducted, in order to establish the identification method and content determination method and solve the problem of its authenticity, and to analyze the reasons for its different clinical efficacies. The methods of primary identification, morphological identification, microscopic identification, TLC identification and HPLC were used in the pharmacognostical study of B. aurea. The results were as follows:(1)It is a perennial stout woody vine with reddish brown hairs on the surface of stems, leaves, flowers and fruits.(2)A large number of stone cell groups and crystal fiber could be seen in the cortex of the transverse section of stems. The calcium oxalate cluster crystals and square crystals were scattered in the parenchyma of pith. The cross section of the leaves showed that the leaves were bifacial leaves. Calcium oxalate cluster crystals and square crystals were commonly found in the cortex. Secretory cavities were arranged intermittently into a ring in the phloem. Crystal fibers were often seen around the phloem. Calcium oxalate cluster crystals, crystal fibers, stone cells, bordered pit vessels, non-glandular hairs and stomata could be found in the powder.(3)The spots of samples were displayed in the same color as the reference substances in the same position of the TLC figure.(4)The linear ranges of astilbin and quercitin were 0.005 888-2.355 μg(r=0.999 6)and 0.039 55-1.582 μg(r=0.999 8). The average recoveries(n=6)were 96.42%(RSD=2.55%)and 104.2%(RSD=1.79%). In this study, pharmacognostical identification methods and a HPLC method for simultaneous content determination of two components astilbin and quercitin in B. aurea were developed. The methods are simple, stable, accurate, which can be used as the basis to set up its quality standard.]]> 2023/3/6 22:38:48 1,2,3, WANG Zhiping^1,2*, LI Linjie^1,2, WANG Yuhan^1,2, WEI Songji¹]]> LIU Xiao^1,2,3, WANG Zhiping^1,2*, LI Linjie^1,2, WANG Yuhan^1,2, WEI Songji¹ 18true Veronica didyma, CCK8 method was used to evaluate the inhibitory effects of four extracts(petroleum ether extract, ethyl acetate extract, n-butanol extract, water extract), ethanol extracts and monomer compounds on the proliferation of melanoma cell lines(B16 and A375). The chemical constituents of the active fraction were systematically separated and purified by phytochemical techniques and methods. The results were as follows:(1)Ethyl acetate extract(PPNE)had a better inhibitory effect on the proliferation of B16 and A375 cells than other samples, and IC₅₀ = 0.177 mg·mL^-1(B16), IC₅₀ = 2.826 mg·mL^-1(A375), respectively.(2)Seven monomer compounds were obtained from PPNE were p-hydroxybenzaldehyde(1), picroside II(2), isoscutellarein 7-O-(6-oacetyl)-β-allopyranosyl(1→2″)-β-glucopyranoside(3), 3'-hydroxyl-4'-O-methylisoscutellarein 7-O-[6-O-acetyl-β-D-allopyranosyl-(1→2)-β-D-glucopyranoside(4), 6-O-veratroylcatalposide(5), veronicoside(6), isoscutellarein 4'-methyl ether 7-O-(6-O-acetyl)-ballopyranosyl(1→2″)-β-glucopyranoside(7).(3)Seven compounds were isolated from this plant for the first time, and HPLC showed that these seven compounds were the major components of PPNE.(4)Except Compound 1, the other six monomers all had good inhibitory effects on the proliferation of melanoma cells, and the anti-melanoma activities of compounds 3, 4 and 7 were reported for the first time. Based on these results, PPNE of V. didyma is an anti-melanoma active fraction, iridoids(compounds 2, 5, 6)and flavonoids(compounds 3, 4, 7)may be the basis of anti-melanoma activity of PPNE. The results of this study will lay scientific foundation for the rational utilization of V. didyma's resources.]]> 2023/3/6 22:38:48 1, ZHANG Yuanfang¹, LI Linghuan², PENG Zhonglu¹, WANG Junjie^1*]]> LIU Fang¹, ZHANG Yuanfang¹, LI Linghuan², PENG Zhonglu¹, WANG Junjie^1* 17true Artemisia argyi var. argyi ‘Qiai', and the antibacterial activities of their secondary metabolites were the main focus of this study. The tissue culture method was used to isolate the endophytic bacteria from roots, stems and leaves of Artemisia argyi var. argyi ‘Qiai' and then, the antibacterial activity of the volatiles from endophytic bacterial fermentation broth against six common pathogenic bacteria was assayed by the disk diffusion test. Meanwhile, the minimum inhibitory concentrations(MICs)and the minimum bactericidal concentrations(MBCs)were also determined. Finally, the isolated strains were identified based on phenotypical and biochemical properties as well as 16S rRNA gene sequencing.The results showed that,(1)among the thirteen strains isolated, strains lzy-21, lzy-20 and lzy-1 have strong capacity of producing cellulase, protease and lipase respectively.(2)The MICs of the volatiles of lzy-20 and wnn4-3 to Escherichia coli, Enterobacter aerogenes and Bacillus subtilis were all 16 μg·mL^-1. The MBCs of the them were 32, 32, 16 μg·mL^-1 and 16, 32, 32 μg·mL^-1, respectively.(3)The MICs of lzy-12 against Staphylococcus aureus and Yersinia enterocolitica were 16 μg·mL^-1, and the MBCs of them were 32 μg·mL^-1 and 16 μg·mL^-1, respectively.(4)The MICs of both lzy-17 and lzy-21 against Y. enterocolitica were 16 μg·mL^-1, and the MBCs were 16 μg·mL^-1 and 32 μg·mL^-1 respectively. Strain lzy-1 was identified as Acinetobacter pittii, which was isolated from plants for the first time. lzy-20, lzy-21, wnn4-3, lzy-12, and lzy-17 were identified as Bacillus altitudinis, B. koreensis, B. siamensis, Luteibacter pinisoli, and Paenarthrobacter nicotinovorans, respectively. The above results lay a foundation for making full use of endophytic bacteria resources to produce industrial enzymes, and further exploring the mechanisms of how endophytic bacteria interact with Artemisia argyi var. argyi ‘Qiai'.]]> 2023/3/6 22:38:48 *]]> XU Bilin, LUO Zhouyu, PENG Chenwanli, WU Hui, ZHANG Ziye, ZHENG Yue, ZHENG Yongliang^* 16true Psammosilene tunicoides is an important ethnic medicine in Southwest China and the main raw material for Chinese patent medicines like Yunnan Baiyao. The purpose of this study was to isolate and identify the pathogenic fungus of root rot of Psammosilene tunicoides in Yunnan. In this study, the disease-health junction of the root tissue of diseased plant was isolated and purified by pure culture method to obtain the main pathogenic microorganisms of P. tunicoides. Pathogenicity of isolated pathogenic microorganisms was analyzed the according to Koch's Postulates, and then were identified by morphological observation, rDNA-ITS and TEF-1α sequence analysis and phylogenetic tree construction. The results were as follows:(1)A total of 85 strains of fungi were obtained, among which PSD-1, PSD-2 and PSD-3 of Fusarium fungi caused root rot of Psammosilene tunicoides.(2)These three strains were re-inoculated into the P. tunicoides, which produced similar disease symptoms occurred with field plants, and the disease incidences after inoculation with pathogenic fungi were 60%, 61.7% and 71.7%, respectively.(3)Combined with morphological observation and gene sequence analysis, the three strains were identified as Fusarium oxysporum. This study firstly found that the F. oxysporum was one of the main pathogenic fungi causing root rot of Psammosilene tunicoides. The results of this study lay a foundation for the in-depth exploration of the endophytic flora with biological control of root rot and its mechanism, and serve as a theoretical basis for the follow-up prevention and control of the disease.]]> 2023/3/6 22:38:48 *]]> YANG Wenting, ZHANG Xiaomei, LI Guodong, LI Junnan, YANG Chengfan, ZHANG Aili^* 15true Polygala fallax has a variety of biological activities. In order to fully develop the endophytic fungi resources of the wild P. fallax and to obtain the culture-endophytic fungi strains with an anti-phytopathogenic fungi and antioxidant activities, the endophytic fungi of P. fallax were taken as the research object, the antifungal activity of the endophytic fungi against six kinds of phytopathogenic fungi was detected by the plate confrontation method; and the antioxidant activity of endophytic fungi was evaluated by measuring the DPPH free radical scavenging ability and total reducing ability of endophytic fungi fermentation broth. Finally, the endophytic fungi strains with strong antifungal and antioxidant activities were then analyzed by the morphological traits and the flanking ITS sequencing. The results were as follows:(1)In the antifungal experiment, there were two endophytic fungi of P. fallax had obvious antifungal activities on six kinds phytopathogenic fungi of Fusarium oxysporum f. sp. cubense, Phyllosticta zingiberi, Diaporthe citri, Mycosphaerella fijiensis, Fusarium solani, F. oxysporum, and the inhibition rates ranged from 50.3% to 91.4%. Among them, HNLF-5 strain had a good effect antifungal activity against Diaporthe citri, with a inhibition rate of 73.2%, and HNLF-44 strain had a good effect antifungal activity against Fusarium oxysporum f. sp. cubense, with a inhibition rate of 91.4%.(2)In the experiment of antioxidant activity, the endophytic fungal fermentation broth had good antioxidant activity, the DPPH clearance rate was above 80%, and the total reducing ability absorbance value ranged from 0.279 2 to 0.748 8.(3)It could be seen from the strain identification results that the HNLF-44 strain with antifungal activity was Alternaria sp. fungus. This study indicates that the endophytic fungi of Polygala fallax have a good ability of inhibiting plant phytopathogenic fungi and antioxidant activity, which lays the foundation for the subsequent exploration of potential new antifungal and antioxidant substances from P. fallax endophytic fungi.]]> 2023/3/6 22:38:48 1,3, LI Huimin³, TANG Hui^1,2*, LI Liangbo⁴, LIU Baoyu^1,2]]> SU Xiuli^1,3, LI Huimin³, TANG Hui^1,2*, LI Liangbo⁴, LIU Baoyu^1,2 14true Polygala fallax, the study analyzed the root morphology, biomass, medicinal components, nutritional components and mineral elements of P. fallax roots during the 1-5 cultivating years by national standards and other methods. The results were as follows:(1)The morphology and biomass of P. fallax roots increased slowly during the first two cultivating years, and increased rapidly during the 2-3 cultivating years, however the parameters tended to be flat during the 3-5 cultivating years.(2)The contents of different medicinal ingredients varied significantly within the cultivating years, crude polysaccharides reached to the peak in the first year, total saponins reached to the peak in the second year, and total flavonoids reached to the peak in the fourth year.(3)The mineral elements showed various behavior among cultivating years, the contents of Ca, Mg, Mn and Cu accumulated to the maximum in the third year, the content of Fe decreased with increasing cultivating years, and the content of Pb increased with increasing cultivating years.(4)Crude protein content increased first and then decreased, and the same change trends were observed in the contents of total ash and crude fat, both of which decreased as cultivating years increasing.(5)P. fallax reached a high yield in the third cultivating year, and most of the quality indicators in the roots of 4-5 year at the lowest value, and the third year was the ideal harvest period for P. fallax, which provided technical guidance for high-yield and good quality of P. fallax. The results of the study elucidate the dynamics accumulation of P. fallax during different cultivating years and reveal the optimum harvest time of P. fallax.]]> 2023/3/6 22:38:48 1,2, LI Dianpeng^2,3*, TANG Hui^2,3, LIU Baoyu^2,3, WANG Manlian^2,3, LU Fenglai^2,3, Zheng Yumei^1,2]]> WAN Li^1,2, LI Dianpeng^2,3*, TANG Hui^2,3, LIU Baoyu^2,3, WANG Manlian^2,3, LU Fenglai^2,3, Zheng Yumei^1,2 13true Stephania kwangsiensis, a vine plant of the Stephaniaceae family, is mainly spread in Guangxi, China. The medicinal part(root tuber)of S. kwangsiensis, “Jin Bu Huan” in Chinese, is often used for sedative, insecticidal, anti inflammatory, anti-tumor, antiviral and antibacterial, as well as for the treatment of Alzheimer's disease. In order to make full use of the plant resources, clarify its chemical constituents, and find the relevant active compounds, the methanol extracts of non-medicinal parts of this plant were isolated using various chromatographic methods such as positive phase Sephadex LH-20, positive phase silica gel, positive phase polyamide, and MCI gel CHP-20P. The structures of the isolated monomer compounds were determined by MS, ¹H-NMR, ¹³C-NMR, and other spectral data analysis, as well as comparison with relevant literatures. The activities of the isolated compounds from S. kwangsiensis against bacteria(Escherichia coli, Staphylococcus aureus, Bacillus subtilis)and fungi(Fusarium oxysporum)were evaluated using filter paper method. The results were as follows:(1)A total of 10 compounds obtained from the non-medicinal parts of this plant were identified as vomifoliol(1), grasshopper ketone(2), isocorydine(3),2-O-β-D-glucopyranosyl-D-arabinitol(4),(9S,12S,13S)-trihydroxyoctadeca-(10E,15Z)-dienoic acid(5), magnoflorine(6), corchoionoside C(7), tormentic acid(8), corydine(9)and crebanine(10). Among them, compounds 1, 2, 4, 5, 6, 7 and 8 were isolated from this plant for the first time, compounds 1, 2, 4, 5, 7 and 8 were firstly obtained from this genus plants, and Compound 4 was found from natural products for the first time.(2)At concentration of 6 mg·mL^-1, compounds 1, 2 and 5 showed weak antibacterial activities against Escherichia coli; Compound 2 exhibited weak antibacterial activities against Staphylococcus aureus; compounds 3 and 8 displayed weak antibacterial activities against Bacillus subtilis. In conclusion, the non-medicinal parts of Stephania kwangsiensis contain large numbers of active components and have the potential of development and utilization.]]> 2023/3/6 22:38:48 1,2, LI Xia¹, HUANG Yonglin², WANG Yafeng², YANG Bingyuan², LI Dianpeng², HE Ruijie^2*]]> ZHANG Rusheng^1,2, LI Xia¹, HUANG Yonglin², WANG Yafeng², YANG Bingyuan², LI Dianpeng², HE Ruijie^2* 12true Corydalis saxicola which belongs to the family of Papaveraceae, is always used to treat various diseases such as hepatitis B, cirrhosis, furuncle, acute icteric hepatitis, and advanced cancer as traditional Zhuang medicine in Guangxi province. To study the anti-inflammatory activity of the chemical constituents of C. saxicola, silica gel, MCI, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography were used to separate the chemical constituents from the ethyl acetate fraction of 95% ethanol extract of C. saxicola. Their structures were identified by nuclear magnetic resonance(NMR)and high resolution mass spectrum(HR-MS). In addition, the anti-inflammatory activity was evaluated by the mouse macrophage RAW264.7 induced by lipopolysaccharide(LPS). The results showed that 13 chemical compounds were isolated and identified as coptisine(1), berberine hydrochloride(2), crinumaquine(3), worenine(4), dehydrocavidine(5),(-)-tetrahydropalmatine(6), jatrorrhizine(7), corydaldine(8), N-trans-feruloyl tyramine(9), dibutylterephthalate(10), kaempferol(11), isololiolide(12), loliolide(13)respectively. Compounds 3, 4, 8-13 were isolated from this plant for the first time. Compounds 4, 9 and 11 showed good inhibitory effects on LPS induced NO production by macrophage RAW264.7 with IC₅₀ values of(18.8 ± 0.2),(29.1 ± 0.3),(18.0 ± 0.1)μmol·L^-1 respectively, which was better than the positive control indomethacin, exhibited good potential in the research and development of anti-inflammatory drugs. These results can clarify the anti-inflammatory material basis of C. saxicola, and provide a scientific theoretical basis for its further utilization.]]> 2023/3/6 22:38:48 1,2, LI Ling¹, QIN Feng¹, HE Jiman¹, TANG Wendi¹, GENG Lixiao¹, QIN Jiangke^1*]]> OUYANG Zhiwei^1,2, LI Ling¹, QIN Feng¹, HE Jiman¹, TANG Wendi¹, GENG Lixiao¹, QIN Jiangke^1* 11true Champereia manillana var. longistaminea, the chemical composition was analyzed by the silica gel column chromatography(CC), thin-layer chromatography(TLC), dextran gel column chromatography(Sephadex LH-20), reversed-phase silica gel(RPC₁₈)column chromatography and high performance liquid chromatography(HPLC). The ethyl acetate extraction part of the ethanol extraction was separated and six monomer compounds were obtained. The structures of these compounds were identified using nuclear magnetic resonance spectroscopy(NMR), high resolution mass spectrometry(HR-ESI-MS)and the data of the literature. Six compounds were elucidated as taraxerol(1), 1H-indole-3-carboxylic acid(2),(24R)-cycloartane-3β, 24, 25-triol(3),(24R, S)-3β-24,31-epoxy-24-methylcycloartane(4), 1-O-linolenoyl-3-O-β-D-galactospyranosyl-snglycerol(5), and hyloglyceride(6). All compounds 1-6 were isolated from this plant for the first time.]]> 2023/3/6 22:38:49 1, ZOU Rong², TANG Jianmin², WEI Xiao², SUN Zhirong¹, SHI Yancai^2*]]> ZHU Chenghao¹, ZOU Rong², TANG Jianmin², WEI Xiao², SUN Zhirong¹, SHI Yancai^2* 10true Heynea trijuga, silica gel, MCI, C₁₈, Sephadex LH-20 and preparative HPLC were applied for the isolation and purification of the constituents. The structures were identified by physicochemical properties and spectrum data. The results were as follows:(1)Six compounds were obtained from methanol extracts of H. trijuga, their structures were identified as heynealigan A(1), 3β,21-dihydroxy-24-methylenecycloartane(2), 3-oxo-threo-23,24,25-trihydroxytirucall-7-ene(3), 16-dehydeoxy-23β-hydroxyliasenin F(4), ergost-5, 24(28)-diene-3β, 23S-diol(5), 7α-hydrositosteol(6).(2)Compound 1 was a new lignan, compounds 2-4 were triterpenoids, compounds 5 and 6 were steroids, and compounds 2-5 were isolated from this plant for the first time. This study provides scientific basis for the rational development and utilization of T. connaroides.]]> 2023/3/6 22:38:49 1, YAN Xiaoyan¹, PENG Mingyou¹, ZHANG Xiong¹, WANG Jianta¹, TANG Lei¹, YAN Ying^1,2*]]> WANG Dan¹, YAN Xiaoyan¹, PENG Mingyou¹, ZHANG Xiong¹, WANG Jianta¹, TANG Lei¹, YAN Ying^1,2* 9true Xanthium strumarium is a traditional folk medicinal plant. The 50% ethanol eluting faction of X. mongolicum had significant activity to inhibit necroptosis. In order to investigate the active constituents, the active fraction of X. mongolicum were isolated and purified by various chromatographic methods such as repeated silica gel column chromatography, Sephadex LH-20 column chromatography, preparative high performance liquid chromatography and recrystallization. Their structures were established on the basis of NMR, MS spectraoscopic analyses and comparison with lirerature date. The results were as follows: A total of 14 compounds were isolated from the active part and identified as hydroxydihydrobovolide(1), raspberry ketone(2), salicyl alcohol(3), 4-hydroxyl-acetophenone(4), 4-hydroxybenzaldehyde(5), ethyl caffeate(6), ferulaldehyde(7), isoscopletin(8), 3,3'-bis(3,4-dihydro-4-hydroxy-6-methoxy-2H-1-benzopyran)(9), axillarin(10), quercetin(11),(+)pinoresinol(12), β-sitosterol(13)and palmatic acid(14). Compounds 1-4, 7-10 were all isolated from Xanthium mongolicum for the first time.]]> 2023/3/6 22:38:49 1, FU Lulu¹, XU Junshen¹, SHANG Ruifeng¹, CHEN Zhongwen¹, LIU Feng², LIU Hua^1*]]> SHENG Tianlu¹, FU Lulu¹, XU Junshen¹, SHANG Ruifeng¹, CHEN Zhongwen¹, LIU Feng², LIU Hua^1* 8true Indigofera stachyodes, the petroleum ether part of this plant was separated and purified using silica gel column chromatography, Sephadex LH-20 column chromatography, semi-preparative high performance liquid chromatography, and recrystallization methods. The structures of obtained monomeric compounds were identified through modern spectral techniques by detail analysis of their electrospray ionization mass spectrometry, hydrogen and carbon nuclear magnetic resonance spectra data, as well as compared with the reported references. The results were as follows: twenty-two natural compounds were identified as β-sitostenone(1), stigmasta-3,6-di-one(2), 6β-hydroxystigmast-4-en-3-one(3),(22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(4), medicarpin(5), sativan(6), 2',4'-dihydroxychaicone(7), 6,7-dimethoxy-4-hydroxy-1-naphthoic acid(8), p-hydroxybenzoic acid ethyl ester(9), 2,4-dihydroxybenzoic acid ethyl ester(10),(9E,11E)-13-oxo-9,11-ocatadecadienoic acid(11),(9E,11E)-13-oxo-9,11-octadecadienoic acid methyl ester(12), 9-oxo-10E,12E-octadecadienoic acid methyl ester(13), 9-hydroxy-10E,12Z-octadecadienoic acid methyl ester(14), pinellic acid(15)and 9-oxo-10E,12E-octadecadienoic acid-(2S)-2,3-dihydroxypropyl ester(16), β-sitosterol(17), betulinic acid(18), 3β-hydroxyolean-12-en-11-one(19), maackiain(20), phellopterin(21), palmitic acid(22). Of these compounds, ten compounds(compounds 1-3, 7, 11-16)were isolated from the Fabaceae for the first time; sixteen compounds(compounds 1-16)were obtained from Indigofera plant for the first time; at the same time, compounds 1-16, 18, 19, 21 were obtained from the roots of Indigofera stachyodes for the first time. Thus, the results of this research enrich the chemical structure types of roots of I. stachyodes, and afford a scientific foundation for its further rational use.]]> 2023/3/6 22:38:49 1,2, ZHOU Yao^1,2, ZHANG Can¹, LIU Qing³, ZHOU Ping³, LI Jinyu^1,2, LOU Huayong^1,2, PAN Weidong^1,2*]]> WANG Huan^1,2, ZHOU Yao^1,2, ZHANG Can¹, LIU Qing³, ZHOU Ping³, LI Jinyu^1,2, LOU Huayong^1,2, PAN Weidong^1,2* 7true Nerium oleander is an important medicinal plant. In order to study the diversity of endophytic fungi from N. oleander and evaluate the activity of its secondary metabolites, the endophytic fungi of N. oleander in Guangxi were isolated and purified, and identified by a combination of morphology and ITS sequence analysis. Then the antibacterial activities of endophytic fungus extracts were screened with five indicator bacteria(including three Vibrio species). The results were as follows:(1)A total of 19 endophytic fungi were obtained from Guangxi N. oleander. The ITS sequence analysis showed that these 19 endophytic fungi all belonged to the Ascomycota, and covered five orders and seven genera, which including Colletotrichum, Guignardia, Phyllosticta, Neofusicoccum, Aspergillus, Nothophoma and Diaporthe. Among them, the dominant genera were Colletotrichum(the separation rate was 36.85%)and Guignardia(the isolation rate was 21.05%). Colletotrichum was mainly distributed on stems, and Guignardia all originated from leaves.(2)Antibacterial experiments showed that jing-117(Neofusicoccum sp.)and ye-130(Guignardia sp.)had specific antibacterial effects on Vibrio campbellii, ye-136(Aspergillus sp.)could simultaneously inhibit Bacillus cereus and Vibrio campbellii. ye-135(Aspergillus sp.)and jing-116(Colletotrichum sp.)could only inhibit B. cereus, and ye-134(Guignardia sp.)had an inhibitory activity to Vibrio alginolyticus. The study revealed for the first time that endophytic fungi of N. oleander in Guangxi has a relatively rich diversity based on the ITS sequence. Some antibacterial active strains could be screened, and their extracts could inhibit the growth of aquatic pathogen Vibrio, which will has good development value in the future.]]> 2023/3/6 22:38:49 1, LUO Meimiao¹, LI Lu¹, YANG Zicong¹, WANG Lin¹, ZHUGE Caizhi¹, QIU Qinghua², ZHU Kaixin³, SU Benwei³, GONG Bin^1*]]> SONG Jingjing¹, LUO Meimiao¹, LI Lu¹, YANG Zicong¹, WANG Lin¹, ZHUGE Caizhi¹, QIU Qinghua², ZHU Kaixin³, SU Benwei³, GONG Bin^1* 6true Clerodendrum cyrtophyllum is a common medicinal plant used by Miao people. In order to study the chemical constituents and antibacterial activities of C. cyrtophyllum in Guizhou, China. Macroporous adsorption resin, reversed phase column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatagraphy, and semi-preparative high performance liquid chromatography were used to separate and purify 95% ethanol extracts from C. Cyrtophyllum. The antibacterial activities were evaluated by MTT method. The results showed 18 compounds were isolated and identified as friedelin(1), α-amyrin palmitate(2), sambuculin A(3), 22-dehydroclerosterol(4), isopetasin(5), taraxasteryl palmitate(6), stigmasterol(7), 22-dehydroclerosterol-3-O-β-D-(6'-O-margaroyl)-glucopyranoside(8), 3-O-β-D-galactopyranosyl-(24β)-ethylcholesta-5,22,25-trien(9), cistanoside D(10), tortoside F(11), balanophonin(12), luteoloside(13), acteoside(14), luteolin(15), acantrifoside E(16), trichotomside B(17), cistanoside C(18). Among of them, compounds 2, 3, 5, 6, 8-13, 16-18 were all isolated from C. cyrtophyllum for the first time. Besides,biological activity test showed Compound 13 had antibacterial activity against Streptococcus uberis. The study enriches the chemical composition of C. cyrtophyllum, and provides reference for the drug development of anti-mastitis of dairy cows.]]> 2023/3/6 22:38:49 1,2, LI Lijun¹, RAN Jianqiang¹, XUE Zhan^1,2, LIU Lina³, TAN Chengjian^1*]]> HUO Xiaomin^1,2, LI Lijun¹, RAN Jianqiang¹, XUE Zhan^1,2, LIU Lina³, TAN Chengjian^1* 5true n-BuOH part of the bark of Taxotrophis ilicifolia, sixteen compounds were isolated and purified from the n-BuOH part of the bark of T. ilicifolia by means of various column chromatographic techniques, including silica gel, ODS, Sephadex LH-20 and semi-preparative RP-HPLC. The structures of the isolates were identified by physiochemical properties and spectral data. The results were as follows: The compounds were identified as icariside E5(1), secoisolariciresinol 9-O-β-glucopyranoside(2), 2,4,6-trimethoxyphenol-1-O-β-D-glycoside(3),9-O-β-glucopyranosyl trans-cinnamyl alcohol(4), 3,4,5-trimethoxyphenyl-1-O-β-apiofuranosyl-(1″→6')-β-glucopyranoside(5),3-hydroxy-4,5-dimethoxyphenyl-β-D-glucopyranoside(6), 2,6-dimethoxy-4- hydroxyphenol-1-O-β-D-glucopyranoside(7), isotachioside(8), ficuscarpanoside A(9), uridine(10), methyl syringate 4-O-β-D-glucopyranoside(11), 3,4,5-trimethoxyphenyl-β-D-glucopyranoside(12), luteolin(13), ginsenoside Rg1(14),(+)-lyonirenisol-3α-O-β-D-glucopyranoside(15), myricetin 3-neohesperidoside(16). All compounds were isolated from plants of Taxotrophis for the first time.]]> 2023/3/6 22:38:49 1, 2, HUANG Xishan², HUANG Yan², LAI Hongfang¹, Wang Aijuan¹, LI Jun²]]> ZHANG Gaorong^{1, 2}, HUANG Xishan², HUANG Yan², LAI Hongfang¹, Wang Aijuan¹, LI Jun² 4true Trachelospermum jaminoides, a liana of family Apocynaceae, is used as classic traditional Chinese medicine recorded in the Chinese Pharmacopoeia, and Ficus tikoua, a liana of family Moraceae, is used as its customary supply in some ethnic districts, both of which are used as remedies for the inflammation-related disease. In order to determine the differences of anti-inflammatory activity and related chemical constituents between the two medicinal materials, the cell viability test was used to detect the cytotoxicity(RAW264.7)of the medicinal parts in T. jaminoides and F. tikoua at different concentrations, and the Griess method was applied to detect the inhibitory effects of the two species on nitric oxide(NO)production in LPS-induced RAW264.7 cells in vitro. The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)combined with the OPLS-DA model in multivariate statistics were used to identify the differential chemical constituents between the medicinab parts in T. jaminoides and F. tikoua. The results were as follows:(1)The NO production inhibition rate of T. jaminoides is stronger than that of F. tikoua.(2)Twenty-one differential chemical components were identified in the stems with leaves of T. jaminoides, most of which were lignans. Ten differential chemical components in the aerial parts of F. tikoua, most flavonoids, were identified. In summary, the differential chemical constituents may be the main active substances that cause the difference in anti-inflammatory activity between the two medicinal materials. This study provides a theoretical basis for the anti-inflammatory medicinal material basis and quality control of the medicinal parts in T. jaminoides and F. tikoua.]]> 2023/3/6 22:38:49 1, SONG Hongzhi¹, Edward J. Kennelly², TAN Qingang^1,2*]]> HUANG Zihong¹, SONG Hongzhi¹, Edward J. Kennelly², TAN Qingang^1,2* 3true Mahonia fortunei by macroporous resins, the best resins were selected by comparing the static adsorption and desorption effects of six kinds of macroporous adsorption resins on total alkaloids. The optimum process conditions for the dynamic purification of total alkaloids were investigated, and the anti-oxidation performance of total alkaloids before and after purification was evaluated by DPPH method. The results were as follows:(1)AB-8 macroporous adsorption resins had the best purification effects. The optimum process conditions were crude herbal dose of 50 mg·mL^-1, sample volume of 26 BV, sample flow rate of 2 BV·h^-1, eluted successively with 3 BV water and 4 BV 50% ethanol. Under these optimized conditions, the content of total alkaloids was increased from 13.33% to 56.64%.(2)The total alkaloids had good scavenging effects on DPPH· before and after purification. The scavenging rate of DPPH· was Vc(IC₅₀=10.39 μg·mL^-1)> purified total alkaloids(IC₅₀=39.08 μg·mL^-1)> crude total alkaloids(IC₅₀=55.28 μg·mL^-1). AB-8 macroporous adsorption resin can effectively enrich the effective parts of total alkaloids from leaves of Mahonia fortunei, and the total alkaloids have certain antioxidant activities.]]> 2023/3/6 22:38:49 *]]> JIA Kai, LIU Jun, GENG Xiaotong, ZHANG Yaozhou, XIAO Ying^* 2true Ailanthus altissima, the silica gel and Sephadex LH-20 were employed to separate and purify methanol extract from the root bark of the A. altissima, and the structures of the compounds were identified by chemical properties and spectral data. Flow cytometry was employed to test the antibacterial activity of the compounds, and kanamycin was used as control group. The results were as follows: Twenty-two compounds were isolated and elucidated from the root bark of A. altissima respectively as pleuchiol(1), withastramonolide(2), 7-ketositosterol(3), betulin(4), betulinic acid methyl ester(5), 1, 2, 4-trimethoxybenzene(6), dimethyl maleate(7), sonderianol(8), dibutyl phthalate(9), pinoresinol(10), p-hydroxybenzoic acid ethyl ester(11), avenalumic acid methyl ester(12), 5, 3'-dihydroxy-3,7, 4'-trimethoxyflavone(13), spathulenol(14), 2-methyl-5-acetonyl-7-hydroxychromone(15), 7, 4'-dihydroxyflavone(16), annphenone(17), 3-hydroxy-4-methoxybenzoic acid(18), 5, 3',4'-trihydroxy-7-methoxyflavanone(19), dibutyl phthalate(20), 4-O-methylgallic acid(21), dioctyl terephthalate(22). All compounds were isolated from the root bark of A. altissima for the first time. The antibacterial activity tests showed that Compound 2 had inhibitory effect on Pseudomonas aeruginosa and Bacillus subtilis. Compound 3 had inhibitory effects on Bacillus subtilis. Compound 8 had inhibitory effects on Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Compound 17 had inhibitory effects on Pseudomonas aeruginosa and Staphylococcus aureus. The inhibitory effect of Compound 2 on Bacillus subtilis was not significantly different from kanamycin(P>0.05). This paper aims to clarify the antibacterial substance basis of the root bark of A. altissima, and provide a certain theoretical basis for the development and utilization of the root of A. altissima resources and the research and development of drugs with antibacterial activity.]]> 2023/3/6 22:38:49 *, WANG Fengxia, MING Hubin, HE Peina, WANG Jihong]]> YUAN Yamin, ZHOU Xiaohuan^*, WANG Fengxia, MING Hubin, HE Peina, WANG Jihong 1true