Nervilia fordii as well as in the root-associated soil, we collected the terrestrial orchids grown in Daxin(DX)County and Longzhou(LZ)County of the karst areas in Guangxi Zhuang Autonomous Region, China as well as the root-associated soil, respectively. We analyzed the composition of fungal communities in the roots and the root-associated soil by taking the Illumina MiSeq high-throughput sequencing technology. The results were as follows:(1)The fungal diversity in the roots and root-associated soil of DX and LZ were very rich. In addition, the diversity of fungi in root-associated soil was higher than that in roots, and the fungal diversity in the taproot was higher than that in walking stem.(2)A total of 118 040 valid sequences and 207 operational taxonomic unites(OTUs)were obtained. The BLAST results showed that these OUTs involved 8 phyla, 19 classes, 42 orders, 86 families and 123 genera.(3)The dominant fungal communities in roots of two areas(DX and LZ)both were Basidiomycota including Tulasnellaceae, Trimorphomycetaceae, Ceratobasidiaceae and Malasseziaceae as well as Marasmiaceae. Among them, Tulasnellaceae(75%)and Epulorhiza(Sexual type: Tulasnella)(56%)were the dominant family and genus, respectively. However, Fusarium was the dominant fungal genus in the root-associated soil. In conclusion, there is a significant difference in fungal communities between roots and root-associated soil, but there is some common OTUs. More importantly, our results also suggest that these fungi species belonging to Epulorhiza may play an important effect on the seed germination and seedling growth of Nervilia fordii grown in the karst area of Guangxi.]]> 2023/4/17 9:57:58 1, YANG Xinfeng¹, SUN Xueping¹, ZHOU Yaqin^1,2*, HU Shiyi¹, YUAN Changge¹, SHI Zhongheng¹]]> TAN Xiaoming¹, YANG Xinfeng¹, SUN Xueping¹, ZHOU Yaqin^1,2*, HU Shiyi¹, YUAN Changge¹, SHI Zhongheng¹ 17true 2023/4/17 9:57:58 1,2,3, WANG Bin^2,3, WEI Yulian^1,2,3, HE Feng^2,3,4, LU Fang^2,3, LI Dongxing^2,3, HUANG Fuzhao^2,3, GUO Yili^2,3, XIANG Wusheng^2,3, LI Xiankun^1,2,3]]> DONG Yanping^1,2,3, WANG Bin^2,3, WEI Yulian^1,2,3, HE Feng^2,3,4, LU Fang^2,3, LI Dongxing^2,3, HUANG Fuzhao^2,3, GUO Yili^2,3, XIANG Wusheng^2,3, LI Xiankun^1,2,3 16true Excentrodendron tonkinense is a constructive species of karst seasonal rainforest and a karst obligate plant, which is also one of the national secondary key protected wild plant and an IUCN endangered plant, with high ecological economic value. In order to explore how the potential suitable areas of E. tonkinense change in the context of global change and its key driving factors, we used the maximum-entropy(MaxEnt)model to analyze the potential geographical distribution changes in China under future climate scenarios(SSP1-2.6 and SSP5-8.5), and tested the influence of the karst geological background distribution on predicting the suitable areas of karst obligate plants. The results were as follows:(1)In the case of adding karst geological background data, the average AUC value of the prediction model for the suitable area was 0.997, which had a good prediction effect. And the model prediction results were strictly limited to the karst region, consistent with the characteristics of karst obligate plant E. tonkinense.(2)According to the fitting results of the model, the karst geological background, the precipitation of the warmest quarter(800-950 mm), and the minimum temperature of the coldest month(7-11 ℃)were the key factors limiting the distribution of E. tonkinense.(3)With the increase of temperature in the future, the potential suitable areas for E. tonkinense would continue to expand in higher latitude karst areas; large areas of stable habitats existed in parts of southwestern Guangxi and southeastern Yunnan. In conclusion, the karst geological distribution is essential as predicting the potential geographic distribution of karst obligate plants such as E. tonkinense; if the temperature continues to rise in the future, its potential suitable areas will expand to high latitudes, and the degree of endangerment may be affected by climate, which means that it is not obvious under the influence of climate change; parts of Southwest Guangxi and Southeast Yunnan are suitable areas for the conservation and utilization of E. tonkinense under the climate change scenarios in the future. The results provide some scientific reference for the introduction, cultivation, sustainable management, protection and utilization of E. tonkinense.]]> 2023/4/17 9:57:58 1,2,3, WANG Bin^2,3, LI Dongxing^2,3, LU Fang^2,3, HUANG Fuzhao^2,3, LI Jianxing^2,3, HE Feng^2,4, XIANG Wusheng^2,3*, CHEN Ting^2,3, LI Xiankun^2,3]]> WEI Yulian^1,2,3, WANG Bin^2,3, LI Dongxing^2,3, LU Fang^2,3, HUANG Fuzhao^2,3, LI Jianxing^2,3, HE Feng^2,4, XIANG Wusheng^2,3*, CHEN Ting^2,3, LI Xiankun^2,3 15true Camellia species under different habitats, 10 species of golden Camellia from calcareous soil habitats and four species from acidic soil habitats were used as the research objects. The calcium content(Soil-Ca)and pH(Soil-pH)of habitat soil, and the contents of calcium nitrate and calcium chloride(AIC-Ca), water soluble organic acid calcium(H₂O-Ca), calcium pectate(NaCl-Ca), calcium phosphate and calcium carbonate(HAC-Ca), calcium oxalate(HCl-Ca), calcium silicate(Res-Ca), and total calcium(Tot-Ca)of leaves were measured. The results were as follows:(1)the Soil-Ca and Soil-pH of calcareous soil were extremely significantly(P<0.01)higher than those of acidic soil.(2)The leaf calcium speciation of golden Camellia species from calcareous soil habitats was primarily HCl-Ca(41.17%), while species from acidic soil habitats was primarily NaCl-Ca(43.10%), and all calcium speciation and total calcium content in leaves of species from calcareous soil were extremely significantly(P<0.01)higher than that from acidic soil except for AIC-Ca and NaCl-Ca.(3)Correlation analysis showed that most of the content of calcium speciation in leaves was extremely significantly(P<0.01)and positively correlated with Soil-Ca and Soil-pH, indicating that soil environment had an important influence on the leaf calcium speciation characteristics of golden Camellia species.(4)According to one-way ANOVA, the content of each leaf calcium speciation was extremely significant(P<0.01)difference among the species in golden Camellia revealing that the calcium speciation characteristics had a considerable variation during the species differentiation.(5)Cluster analysis based on calcium speciation characteristics showed that the 14 species of golden Camellia could be grouped into three categories. In summary, the differences in leaf calcium speciation of plants of golden Camellia species in different habitats may be the consequence of a combination of soil environmental and genetic factors. The results of this study will help to understand the adaptation mechanism of golden Camellia species to soil calcium and provide a reference for the formulation of conservation measures.]]> 2023/4/17 9:57:58 1, TANG Jianmin¹, TAO Ying², QIN Huizhen¹, LIU Kehui^1,2, WEI Xiao¹, CHAI Shengfeng^1*]]> ZHU Xianliang¹, TANG Jianmin¹, TAO Ying², QIN Huizhen¹, LIU Kehui^1,2, WEI Xiao¹, CHAI Shengfeng^1* 14true Prunus salicina economic forest(22 493 grain·m^-2)> Cyclobalanopsis glauca secondary forest(1 033 grain·m^-2)> Phyllostachys sulphurea shrub(793 grain·m^-2).(2)As for the plant life type of the soil seed bank, the soil seed bank life type of Prunus salicina economic forest was mainly annual malignant weeds, the plant life type of the soil seed banks in the Cyclobalanopsis glauca secondary forest and Phyllostachys sulphurea shrub was dominated by perennial herbs, while woody plants account for a relatively small proportion; the species diversity and similarity of the soil seed banks of different vegetation types were generally low, while the species composition of the soil seed banks also had low similarity with the aboveground communities.(3)The soil elements in the study area had higher nitrogen content and lower phosphorus content, and phosphorus was a limiting factor for plant growth in Phyllostachys sulphurea shrub and Quercus glauca secondary forest. In conclusion, the plant community in the stone desertification area of Gongcheng has some native germplasm resources, but overall shows a low species diversity, and it is urgent to introduce native tree species artificially to accelerate the positive vegetation succession on the basis of natural sequestration, and pay attention to nutrient management, especially the regulation of phosphorus elements. The results provide a theoretical reference for karst stone desertification management and vegetation restoration in Gongcheng.]]> 2023/4/17 0:00:00 1,2,3, LI Yufei^1,2,3, WANG Xiaojing⁴, LI Jianxing^2,3, WEN Shujun^2,3, HUANG Fuzhao^2,3, TAN Yanfang^2,3, LU Shuhua^2,3, LU Fang^2,3, CHEN Ting^2,3, GUO Yili^2,3*]]> LI Jiaqi^1,2,3, LI Yufei^1,2,3, WANG Xiaojing⁴, LI Jianxing^2,3, WEN Shujun^2,3, HUANG Fuzhao^2,3, TAN Yanfang^2,3, LU Shuhua^2,3, LU Fang^2,3, CHEN Ting^2,3, GUO Yili^2,3* 13true 2023/4/17 9:57:58 *, XIONG Ling, LI Juan, LIU Qi]]> WU Taohong, LONG Cuiling^*, XIONG Ling, LI Juan, LIU Qi 12true P<0.05), while the topographic factor had no significant effect on the α-diversity of plants in shrub layer.(2)The spatial distribution of plant community species around FAST was most influenced by the altitude, followed by the slope(P<0.05).(3)The Jaccard similarity index above 70% was extremely dissimilar and moderately dissimilar at altitude and slope gradients. The Jaccard similarity index of the three plant communities tended to increase with the increase of altitude and then decrease along the increase of the slope around FAST. In summary, species differed in their habitat selection, and altitude and slope are key factors affecting the spatial distribution of plant communities in the karst depressions around FAST. Species diversity can better reflect the differences in plant communities in terms of species composition. Species diversity is not only influenced by topographic factors, but also related to biotic and abiotic factors, such as temperature, precipitation, human activities, and functional plant traits. Therefore, corresponding environmental factors need to be added in future studies to further investigate the intrinsic mechanisms of plant species diversity and species spatial distribution at the regional scale and to strengthen the ecological protection of the surrounding area, improve the forest composition, and provide a good natural environment around FAST and in the karst peak-cluster depression.]]> 2023/4/17 9:57:58 1, ZHANG Jianli ^1*, YANG Tao¹, ZHANG Chen¹, PU Lihua^1,2, ZHAO Weiquan³]]> ZHANG Ting¹, ZHANG Jianli ^1*, YANG Tao¹, ZHANG Chen¹, PU Lihua^1,2, ZHAO Weiquan³ 11true -2, and the aboveground biomass of dominant species accounted for 91.77% of the whole sample plot. The aboveground biomass of evergreen plants was higher than that of deciduous plants. The aboveground biomass of Lithocarpus confinis and Platycarya strobilacea accounted for the highest proportion in the community, 34.23% and 34.37% respectively.(2)The spatial distribution of rock exposure rate showed obvious upper and lower gradient difference, the upper slope was significantly greater than the lower slope, and the spatial distribution of slope and soil thickness was discontinuous and had no obvious law.(3)There was a significant positive correlation between aboveground biomass and soil thickness, and their spatial distribution tended to be consistent. Soil thickness was the main influencing factor of aboveground biomass of the community, and the influence of rock exposure rate and slope on aboveground biomass of the community was low.(4)For different life forms, the influence of rock exposure rate on aboveground biomass was the highest, and the influence of soil thickness and slope on evergreen plants was greater than that of defoliation.(5)For different species, the correlation between environmental factors and aboveground biomass was complex. Most species were positively correlated with soil thickness. Platycarya strobilacea and Zanthoxylum dimorphophyllum var. spinifolium were positively correlated with rock exposure rate and slope respectively. In conclusion, the vegetation restoration of secondary forest in the study area is slow, and soil thickness is the main influencing factor of aboveground biomass of the community. However, for different life forms and species, the response of aboveground biomass to the environment will be affected by biological factors such as life form differences, species habitat preferences and interspecific relationships.]]> 2023/4/17 9:57:59 1,2, RONG Li^1,2*, WANG Mengjie^1,2, LI Xuan^1,2, YANG Wensong^1,2, WANG Qi^1,2]]> YE Tianmu^1,2, RONG Li^1,2*, WANG Mengjie^1,2, LI Xuan^1,2, YANG Wensong^1,2, WANG Qi^1,2 10true P>0.05)for woody lianas in three different microhabitats. The leaf tissue density for trees was significantly higher at the edge and waist compared to the bottom(P<0.05), while the leaf potassium contents for trees and shrubs at the bottom were higher than that at the edge(P<0.05).(3)There were some differences in the correlation of leaf functional traits in different microhabitats. Leaf thickness was extremely significantly positively correlated with leaf area(P<0.01)at the edge of Tiankeng. The results of principal component analysis suggested that leaf tissue density(-0.833), leaf potassium content(0.782), and leaf dry matter content(-0.647)were the critical indicators for leaf functional traits of woody plants in different microhabitats of Shenmu Tiankeng. In conclusion, there is a general correlation between leaf functional traits, and this study demonstrate that different growth types of woody plants can adapt to the three microhabitats of tiankeng via the trade-off of leaf functional traits. This study is expected to provide theoretical guidance for understanding the adaptation mechanism of plants to special habitats and the protection of tiankeng vegetation.]]> 2023/4/17 9:57:59 1,2,3, LUO Yixing^1,3, XUE Yuegui^1,2,3*, AN Xiaofei^1,2,3, HUANG Gui^1,2,3, LIU Xinyu^1,2,3, CHEN Ming^1,2,3]]> YU Linlan^1,2,3, LUO Yixing^1,3, XUE Yuegui^1,2,3*, AN Xiaofei^1,2,3, HUANG Gui^1,2,3, LIU Xinyu^1,2,3, CHEN Ming^1,2,3 9true 1)There were 21 species, 31 genera and 20 families of woody plants in the inside area of tiankeng, 91 species, 58 genera and 58 families of woody plants in the top area of tiankeng, and 47 species, 30 genera and 30 families of woody plants in the outside area of tiankeng. The Margalef index and Patrick index in the inside areas of tiankeng were significantly lower than those in the top and outside areas of tiankeng, indicating that the individuals of species increased, but the species distribution was steady.(2)The total soil nitrogen content in the outside areas of tiankeng was significantly higher than those in the top and outside areas of tiankeng. The soil total magnesium content, soil phosphorus content and soil available phosphorus content in the inside area of tiankeng were significantly higher than those in the top and outside area of tiankeng. The soil alkaline phosphatase content and soil urease activities in the outside area of tiankeng were significantly higher than those in the top and inside area of tiankeng. The soil phosphorus content in the tiankeng was higher, but the soil enzyme activities were lower.(3)The species diversity was strongly correlated with soil nutrients and soil enzyme activities. In conclusion, it can be seen that the habitats of the tiankeng have a great influence on the species diversity, soil enzyme activity and soil nutrient content distribution characteristics, and the soil available phosphorus content, soil alkaline phosphatase activity, soil moisture content, soil urease activity, soil total magnesium content and soil total phosphorus content are the key factors affecting the species diversity of woody plants in the tiankeng forest. The results provide a theoretical basis for the protection and reconstruction of plant resources in karst areas.]]> 2023/4/17 9:57:59 1,2,3, YU Linlan^1,2,3, CHEN Ming^1,2,3, LIU Xinyu^1,2,3, HUANG Gui^1,2,3, HUANG Linjuan^1,2,3, XUE Yuegui^1,2,3*]]> AN Xiaofei^1,2,3, YU Linlan^1,2,3, CHEN Ming^1,2,3, LIU Xinyu^1,2,3, HUANG Gui^1,2,3, HUANG Linjuan^1,2,3, XUE Yuegui^1,2,3* 8true Malania oleifera,, the mature fruits, leaf, branch bark and bark of M. oleifera collected from Jiaole Tiankeng, Bama County, Hechi City, Guangxi were used as materials in this study. The types and contents of essential nutrients, amino acids and minerals in different parts of M. oleifera were determined by the national standard analysis method of food nutrients in China, and the nutritional values of amino acids in different parts were evaluated. The results were as follows:(1)In terms of essential nutrients, except for naphtha, there were extremely significant differences in the contents of the remaining six essential nutrients among five parts(P<0.01). The ash content of peel was higher than those in other parts, which was 5.7 g·(100 g)^-1. The contents of crude fat and protein in kernel were higher than those in other parts, which were 36.0 and 14.0 g·(100 g)^-1 respectively. The content of vitamin C in leaf was higher than those in other parts, which was 33.9 mg·(100 g)^-1. The content of crude fiber in branch bark was higher than those in other parts, which was 40.5 g·(100 g)^-1. The content of carbohydrate in bark was higher than those in other parts, which was 78.6 g·(100 g)^-1. Naphtha was detected only in kernel and leaf, with the contents of 0.26 and 0.15 mL·(100 g)^-1 respectively.(2)A total of 16 amino acids were detected in the five parts of M. oleifera, including seven essential amino acids. The total numbers of amino acids and essential amino acids of kernel were much higher than those of other parts. In terms of amino acid nutritional value, the nutritional value of kernel and peel were higher than other parts, followed by leaf, branch bark and bark, and the essential amino acid ratio coefficient score(SRC)was 62.98, 59.40, 57.31, 52.25, 48.17, respectively.(3)In terms of macroelements, the highest content of major elements in kernel, peel and leaf was K, the highest contents of major elements in branch bark and bark were Ca, and the contents of Na in five parts of M. oleifera were the lowest. In terms of microelements, the contents of Mn and Fe in five parts of M. oleifera were higher than others. The content of Mn in kernel was the highest, and the content of Fe in branch bark was the highest. In conclusion, the kernel has the highest nutritional value, and other parts also have unique development and utilization potential, which can be effectively and accurately developed and utilized according to the different value characteristics of different parts. The results of this study provide scientific basis and theoretical support for the development and utilization of M. oleifera products in the future.]]> 2023/4/17 9:57:59 1,2, TANG Jianmin¹, SUN Feifei^1,2, QIN Huizhen¹, JIANG Yunsheng¹, WEI Xiao¹, ZOU Rong^1*]]> YANG Yishan^1,2, TANG Jianmin¹, SUN Feifei^1,2, QIN Huizhen¹, JIANG Yunsheng¹, WEI Xiao¹, ZOU Rong^1* 7true Cyclobalanopsis glauca is a constructive population of climax community in karst ecosystems, in order to further understand the spatial distribution pattern of its populations, according to the sample plot investigation specification, a sample plot of 60 m × 60 m was set up, and the woody plants in the plot were spatially located and investigated for each tree. The C. glauca population was divided into three different diameter classes in the study, belonging to seedling(DBH<2 cm), sapling(2 cm≤DBH<10 cm), adult tree(10 cm≤DBH). Using the point pattern analysis method, g(r)and L₁₂ (r)functions based on Ripley's K function were used for the analysis of the spatial distribution pattern and correlation analysis of C. glauca population in different diameter classes. The results were as follows:(1)The diameter class structure of C. glauca population was in an inverted “J” type distribution.(2)On the small scale, the stage of seedlings, saplings and adult trees, the spatial distribution pattern tended to cluster distribution,and the saplings, adult trees tended to random distribution on the medium and large scales.(3)The performances of spatial correlation between different diameter classes were different, the seedlings and saplings were positively related on the scale of 0-15 m; saplings and adult trees reflected on the scale of 0-10 m were no correlation, and there was a positive correlation on the scale of 10-15 m; seedling and adult trees reflected on the scale of 0-2 m were no correlation, and there was a positive correlation on the scale of 2-15 m. Therefore, the spatial distribution pattern and correlation of C. glauca population in the karst area of Guilin change with the spatial scale and development stage, which was the result of the combined effects of the population's biological characteristics, intraspecific and interspecific relationships, and environmental conditions. The results of the study can provide basic information for the formation and maintenance mechanism of plant community and resource utilization research of karst hills.]]> 2023/4/17 9:57:59 2, ZHUO Wenhua^1,3, JIANG Yong^1,3, LIANG Shichu^1,3, LI Lixiang^1,3*]]> PAN Yuanfang², ZHUO Wenhua^1,3, JIANG Yong^1,3, LIANG Shichu^1,3, LI Lixiang^1,3* 6true P_n), stomatal conductivity(G_s), intercellular CO₂ concentration(C_i), transpiration rate(T_r), water use efficiency(WUE)and stomatal limitation value(L_s), by using Li-6400XT portable photosynthesis system. Statistical methods, such as Pearson correlation analysis, principal component analysis(PCA), and permutational multivariate analysis of variance(PERMANOVA)were also used in this study. The results were as follows:(1)Six photosynthetic parameters had different variations within and between species, and all the intraspecific variations were greater than the interspecific.(2)Change of G_s and T_r mainly originated from interspecific variation(46.72% - 49.76%), while that of P_n, C_i, WUE and L_s mainly from intraspecific variation(48.66% - 64.50%). At the life form level, the intraspecific variations of P_n, G_s and T_r of evergreen plants were less than those of deciduous plants, but the intraspecific variation of C_i, WUE and L_s of evergreen plants was higher.(3)Interspecific variations of all the parameters of deciduous plants were greater than those of evergreen plants.(4)G_s variation was the greatest both at the intraspecific and the interspecific levels, followed by T_r and P_n, then L_s and WUE, and C_i variation were the least. There were significant positive correlations among P_n, G_s and T_r(P<0.01). L_s was significantly positively correlated with WUE(P<0.05), but negatively with G_s and C_i(P<0.05). The correlations among these photosynthetic parameters are basically consistent with the global scale, which reflects the diverse trade-off strategies of plants to environment resources. The results also verified the leaf economics spectrum(LES)of karst plant.(5)Evergreen plants were located at the slow investment-return end of the LES with high values of L_s, WUE and low values of G_s, T_r, C_i and P_n. On the contrary, deciduous plants were located at the quick investment-return end of the LES with low value of WUE and high value of P_n and T_r. The results reflect karst plant adapt to changing environment with different adaptation strategies by trade-offs or co-ordinations among traits, and provide scientific basis for selecting adaptive tree species and accelerating the succession process of vegetation restoration in the karst area.]]> 2023/4/17 9:57:59 1, OU Zhiyang^1*, YE Sijin², WU Ziyuan², SHEN Wenhui¹, HE Feng¹, LU Guodao¹]]> PANG Shilong¹, OU Zhiyang^1*, YE Sijin², WU Ziyuan², SHEN Wenhui¹, HE Feng¹, LU Guodao¹ 5true Vitex negundo, Alchornea trewioides and Pyracantha fortuneana)were collected from three positions(upper, middle and lower)along three slopes. The frequency, intensity and abundance of AMF colonization were detected and calculated by the Trypan blue-microscope method. The results were as follows:(1)The colonization frequency, colonization intensity and abundance of AMF for Vitex negundo and Alchornea trewioides were higher than those for Pyracantha fortuneana in the same slope. The colonization intensity for Vitex negundo and Pyracantha fortuneana in the lower slope was higher than that in the upper and middle slopes. The abundance of AMF for Alchornea trewioides in the middle slope was significantly higher than that in the upper and lower slopes(P<0.05).(2)Shrub species had significant effect on the colonization frequency, intensity and abundance of AMF, and slope position only had significant effect on the colonization intensity of AMF. Slope position and shrub species had significant interactive effect on the abundance of AMF(P<0.05).(3)Soil depth and total potassium content were identified as the two strongest explanatory factors on AMF colonization rate according to the redundancy analysis(P<0.05). Therefore, the rational use of soil AMF to promote vegetation restoration in karst areas needs to consider the choice of terrain and species, and should pay attention to the water and soil conservation. The results of this study provide a scientific basis for rational utilization of the symbiotic relationship between soil AMF and plants and promoting ecological restoration of karst peak cluster depression.]]> 2023/4/17 9:57:59 1,2,3, WANG Zhongcheng¹, JIANG Nannan¹, ZHENG Shengmeng^2,3,4, HU Yajun², CHEN Xiangbi², HE Xunyang^2,3*]]> CHEN Meifeng^1,2,3, WANG Zhongcheng¹, JIANG Nannan¹, ZHENG Shengmeng^2,3,4, HU Yajun², CHEN Xiangbi², HE Xunyang^2,3* 4true Wuodendron praecox (Hook. f. & Thomson)B. Xue, Y. H. Tan & X. L. Hou, Phoebe macrocarpa C. Y. Wu, Clematis fengii W. T. Wang, Trichosanthes tetragonosperma C. Y. Cheng & Yueh, Sorbus yunnanensis L. T. Lu, Elatostema longibracteatum W. T. Wang, Acer paihengii Fang, Cornus eydeana Q. Y. Xiang & Y. M. Shui, Rhododendron longipedicellatum Lei Cai & Y. P. Ma, Styrax roseus Dunn, Symplocos hookeri C. B. Clarke, Dicercoclados triplinervis C. Jeffrey & Y. L. Chen, Lysimachia pittosporoides C. Y. Wu, Lycianthes marlipoensis C. Y. Wu & S. C. Huang, Justicia multinodis Benoist, Scutellaria sichourensis C. Y. Wu & H. W. Li, Liparis bistriata C. S. P. Parish & Rchb. f., Peristylus tenuicallus Ormerod, Pinalia yunnanensis(S. C. Chen & Z. H. Tsi)S. C. Chen & J. J. Wood and Zeuxine integrilabella C. S. Leou. Among them, Wuodendron B. Xue, Y. H. Tan & Chaowasku and Dicercoclados C. Jeffrey & Y. L. Chen were new recorded genera of Guangxi. All species were annotated by morphological and distribution description, voucher specimens and pictures. In addition, based on collected materials, several key taxonomic characteristics of Trichosanthes tetragonosperma C. Y. Cheng & Yueh and Dicercoclados triplinervis C. Jeffrey & Y. L. Chen were supplemented and revised respectively. Lastly, the biogeographic significance of these newly records as well as protection effectiveness of the reserves in limestone region of southwestern Guangxi were discussed.]]> 2023/4/17 9:57:59 1,2, LI Man^1,2, WU Wanghui^1,2*, QIN Yonghua¹, LUO Kaiwen¹, WANG Shuangling³]]> LI Xiong^1,2, LI Man^1,2, WU Wanghui^1,2*, QIN Yonghua¹, LUO Kaiwen¹, WANG Shuangling³ 3true 2023/4/17 9:57:59 1, XIAO Zibo^1,2,3, DENG Wei^1,2,3, ZHANG Chao^1,2, TANG Xiaoxin^2,3, JIN Yi^1,2*]]> MAO Yanjiao¹, XIAO Zibo^1,2,3, DENG Wei^1,2,3, ZHANG Chao^1,2, TANG Xiaoxin^2,3, JIN Yi^1,2* 2true Corydalis saxicola is a perennial herbaceous plant, which mainly distributed in the karst areas of southwest China. Due to its important ecological and medicinal value, Corydalis saxicola has great prospects for development and utilization. With fragile living environmental condition and anthropogenic harvesting pressure, the wild resource of Corydalis saxicola is on the verge of exhaustion, so it has been included in the list of national key protected wild plants in 2021. In order to explore the causes for the endangerment of Corydalis saxicola and provide reference for its conservation and seedling breeding, this paper investigated the influencing factors on seed germination that including seed preservation methods, chemical pre-treatments, temperature, light, drought, pH, mix with wet sand and refrigerate treatment. The results were as follows:(1)Low-temperature storage can prolonged the seed activity, germination rate of 2-years stored seed could still reached 30%.(2)Germination rates of seed pre-treatments by HCl and NaClO solutions can reached to 50%～60%; germination rate was 50% at 20 ℃, while closed to zero at 30 ℃; darkness was more favorable for seed germination than periodic light condition; the seed germination rate kept decreasing with deepening drought; the seed germination rate did not changed significantly under the conditions of pH 3.0～8.0.(3)Mix with wet sand and refrigerate treatment can significantly promoted the seed germination potential and germination rate, and the germination rate was twofold of the control. In summary, the seed inactivation at room temperature and germination inability at high temperatures above 30 ℃ are more related to its endangerment; efficient seed germination methods can provide an effective way for conservation and industrial application of Corydalis saxicola. The results provide a theoretical and technical guarantee for the conservation of wild Corydalis saxicola, and technically support is cultivation.]]> 2023/4/17 9:57:59 1,2, GUO Lunfa^1,2*, WANG Xingui¹, ZHANG Yibin¹, QIN Hongbo¹]]> PAN Yanlin^1,2, GUO Lunfa^1,2*, WANG Xingui¹, ZHANG Yibin¹, QIN Hongbo¹ 1true