Pimpinella candolleana is known as Miao ethnic herbal medicine in Guizhou for the treatment of icteric hepatitis, acute cholecystitis and other diseases. To investigate the chemical constituents of P. candolleana and their anti-inflammatory activities, the chemical constituents from the 70% ethanol extract of P. candolleana were separated by silica gel, Sephadex LH-20, Toyopearl HW-40F, Toyopearl HW-40C, ODS and other column chromatography technologies, and their structures were elucidated by extensive spectroscopic analysis such as nuclear magnetic resonance(NMR)and mass spectrum(MS). The inflammatory cell model, built by LPS-induced RAW264.7 macrophage cells, was used to evaluate the anti-inflammatory activity. The results were as follows:(1)Twenty compounds from P. candolleana were isolated and identified of including vanillin(1), sesamin(2), 2-methyl-2-hydroxy-5-methoxy berz(d)hydrofuran-3-one(3), procatechin(4), 1,5-dihydroxy-2,3-dimethoxyxanthone(5), isorhamnetin(6), kaempferol(7), 8-hydroxy-2-methylchromone(8), luteolin(9), quercetin(10), 1-O-β-D-glucopyranosyl-(2S,3S,4R,8E)-2-[(2'R)-2'-hydroxypalmitoylamino]-8-octadecene-1,3,4-triol(11), isorhamnetin-3-O-β-D-galactopyranoside(12), isoquercitrin(13), norswertianolin(14), luteolin-6-C-α-L-arabinoside(15), kaempferol-3-O-β-D-galactopyranoside(16), kaempferol-7-O-β-D-glucopyranoside(17), luteolin-7-O-β-D-glucopyranoside(18), isovitexin(19), rutin(20). Compounds 1, 3, 4, 6, 7, 10, 13, 16, 18, and 20 were obtained from this plant for the first time.(2)The anti-inflammatory results showed that compounds 2-10, 12, 18 and 19 could significantly inhibit the LPS-induced NO content in RAW264.7 cells(P<0.05, P<0.01), and the inhibition rates of compounds 4, 7, 10, and 18 at a concentration of 25 μmol·L^-1 were 57.37%, 83.60%, 68.16%, 81.14%, respectively. Overall, this study enriches the chemical constituents of P. candolleana, and clarifies that flavonoids are the active ingredients in the course of anti-inflammatory, which provides a theoretical reference for further research and exploitation of P. candolleana.]]> 2023/6/27 16:37:33 1,3, LEI Yan^1,3, WANG Yang¹, MA Xue¹, LU Yuan², LIU Chunhua², WANG Yonglin^1,2*]]> LI Li^1,3, LEI Yan^1,3, WANG Yang¹, MA Xue¹, LU Yuan², LIU Chunhua², WANG Yonglin^1,2* 6true Vitex negundo var. cannabifolia, the fingerprints of 18 batches of V. negundo var. cannabifolia leaves were established by high performance liquid chromatography-electrochemical detection(HPLC-ECD), and the cluster analysis of medicinal materials from different sources was performed simultaneously. The main phenolic compounds from V. negundo var. cannabifolia leaves were identified and determined. The contents of total phenolics and total flavonoids in V. negundo var. cannabifolia leaves were analyzed, and the antioxidant activities in vitro were evaluated by the methods of DPPH radical scavenging capacity, ABTS radical scavenging capacity, oxygen radical absorbance capacity and ferric ion reducing ability power. The spectrum-effect relationships of V. negundo var. cannabifolia leaves were analyzed by Pearson correlation analysis, gray relational analysis and partial least square regression analysis. The results were as follows:(1)The fingerprints of V. negundo var. cannabifolia leaves were established with 21 common peaks and a total of 10 peaks were identified. The order of content was chlorogenic acid > isoorientin > luteoloside > isovitexin > isochlorogenic acid A > isochlorogenic acid C > protocatechuic acid > orientin > isochlorogenic acid B > neochlorogenic acid. The similarity among the samples from different producing areas was high, and the values were ranged from 0.816 to 0.983.(2)The results of the cluster analysis showed that the content of the compounds had a certain influence on the classification and the samples from different sources were divided into three categories, among which the samples from the south and the north were different.(3)The contents of total phenolics and total flavonoids in V. negundo var. cannabifolia leaves were 15.82 to 61.83 mg·g^-1 and 27.85 to 157.65 mg·g^-1, respectively, and the antioxidant activity of all samples from V. negundo var. cannabifolia leaves existed differences.(4)The spectrum-effect relationship indicated that the antioxidant activity of V. negundo var. cannabifolia leaves was the result of the synergistic effect of many compounds, and compounds such as peak 9(isoorientin), peak 4 and peak 5(chlorogenic acid)made the greatest contribution to the antioxidant activity of V. negundo var. cannabifolia leaves. V. negundo var. cannabifolia has good antioxidant activity, and the main activity indexes are isoorientin and chlorogenic acid. This study can provide a reference basis for the screening and quality control of antioxidant components in V. negundo var. cannabifolia.]]> 2023/6/27 16:37:33 1, LI Ying¹, LI Zhirong², LIU Mingrong¹, MA Jimin², YU Niannian², CHEN Rongxiang^2,3, ZHOU Yaping^1*]]> WU Qimei¹, LI Ying¹, LI Zhirong², LIU Mingrong¹, MA Jimin², YU Niannian², CHEN Rongxiang^2,3, ZHOU Yaping^1* 5true Callicarpa integerrima has very good effect in emoving blood stasis and resolving static blood and treatment of rheumatism evil. However, there are few reports on its chemical constituents. In order to explore the chemical constituents from the roots and stems of C. integerrima, the 95% ethanol extracts of C. integerrima roots and stems were isolated and purified by diverse column chromatography, such as silica gel, Sephadex LH-20 gel column, ODS column chromatography, and preparative HPLC. In addition, these compounds were identified on the basis of NMR, ESI-MS as well as other modern spectral techniques. The results were as follows: A total of 15 compounds were isolated from the 95% ethanol extracts of the roots and stems of C. integerrima, which were identified as stigmast-4-en-3-one(1),(24R)-5α-stigmastane-3,6-dione(2), 2'-hydroxy-4'-methoxydihydrochalcone(3), α-amyrin(4), β-sitosterol(5), ursolic acid(6), 4-hydroxy-3-methoxy-benzoic acid(7), 4-pyridinol(8), p-hydroxybenzoic acid(9), forsythoside B(10), nepetifosides D(11), isoacteoside(12), acteoside(13), pedicularioside M(14), β-methoxy forsythoside B(15). All compounds, except for compounds 4-6, 12 and 13, were isolated from C. integerrima for the first time. Furthermore, compounds 1, 2, 3, 8, 11 and 14 were isolated from the genus of Callicarpa for the first time. Thus, the results of this research has enriched the compound library and afford a scientific foundation for the further rational use of C. integerrima.]]> 2023/6/27 16:37:33 1,2, SU Zhiwei^2*, MA Zhonghui¹]]> MA Wenjie^1,2, SU Zhiwei^2*, MA Zhonghui¹ 4true α-glucosidase and α-amylase inhibition as indicators, and Pearson correlation analysis was performed. The results were as follows:(1)there were significant differences in the contents of water extract, total polyphenols, total flavonoids and tea polysaccharides among the seven varieties, while the highest contents were found in Golden tea(53.42%±0.14%), Guihong No.4(40.87%±1.09%), Yunnan big leaf species(27.17%±0.26%)and Fuyun No.6(2.70%±0.02%), respectively.(2)There were also significant differences in DPPH· scavenging ability and ORAC values among the seven varieties, while the varieties showing better antioxidant effects in both evaluation methods were the Liupao group species, Guihong No.4 and Wantian species.(3)The inhibition of α-glucosidase and α-amylase by the water extracts of seven varieties was significantly stronger than those of acarbose positive control. The varieties showing better hypoglycemic effects in both evaluation methods were Liupao group species, Guihong No.4 and Guiqing species.(4)The antioxidant and hypoglycemic activities were all strongly and positively correlated with the contents of total polyphenols and flavonoids. In summary, raw tea quality of Liupao group species, Guihong No.4, Wantian species and Guiqing species are better, among which Liupao group species and Guihong No.4 have the prospect of developing antioxidant and hypoglycemic functional food; Wantian species and Guiqing species have the potential of developing antioxidant and hypoglycemic functional food respectively. Total polyphenols and total flavonoids have a great contribution to the in vitro antioxidant and hypoglycemic activities of raw tea, so that attention should be paid to the protection of such components during the further processing and utilization of raw tea. The results of this study provide a scientific basis for the development of Liupao tea products with better antioxidant and hypoglycemic activities in the selection of raw materials and processing methods of raw tea.]]> 2023/6/27 16:37:33 1, CAI Aihua^1*, GUO Chunyu², XIE Yunchang¹, LI Dianpeng¹]]> HUO Huazhen¹, CAI Aihua^1*, GUO Chunyu², XIE Yunchang¹, LI Dianpeng¹ 3true Menispermum dauricum from the Menispermaceae family, is an important Chinese medicinal material. In order to clarify the pharmacodynamic substance basis, the methanol extract of the rhizome in M. dauricum was systematically isolated and purified using various chromatographic methods and the structures of isolated compounds were identified. The potential anti-inflammatory activity of obtained compounds from M. dauricum was measured in vitro. In this study, the chemical constituents were separated via silica gel column chromatography, macroporous adsorption resin and preparative HPLC, and their structures were determined on the basis of MS, ¹H-NMR, ¹³C-NMR and other spectroscopic data analysis, as well as comparison with relevant literatures. Meanwhile, the anti-inflammatory activities of against NO and IL-6 production from LPS-induced RAW264.7 cells of the chemical components were evaluated in vitro. The results were as follows:(1)Fifteen compounds were isolated from the rhizome of M. dauricum, and identified as p-hydroxybenzaldehyde(1), vanillic acid(2), syringaldehyde(3), 2-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(4), methyl 4-hydroxyphenylacetate(5), 2-(4-hydroxyphenyl)-1-nitroethane(6), 4-hydroxyphenylacetonitrile(7), dibutyl phthalate(8), fragransin B₂ (9), 7-hydroxy-3,6-dimethoxy-1,4-phenanthraquinone(10), palmitic acid(11), arachidic acid(12), β-sitosterol(13), β-stigmasterol(14)and daucosterol(15). Among them, compounds 4-7, 9 and 12 were isolated from Menispermaceae for the first time, while compounds 1, 3-11 and 14 were first reported from Menispermum genus. The above compounds were all non-alkaloids components, including phenols, lignans, phenanthraquinones, fatty acids and sterols.(2)Anti-inflammatory assay in vitro showed that Compound 12 could significantly inhibited releases of NO and IL-6 induced by LPS from RAW264.7 cells at the concentrations of 25 and 50 μg·mL^-1, indicating potential anti-inflammatory activity. The results of this study enriches the chemical diversity of the rhizome of M. dauricum and clarifies the active ingredients of its anti-inflammatory activity. It provides an effective reference for the rational utilization of medicinal plant resources.]]> 2023/6/27 16:37:33 1,2, MA Yan¹, CAI Meichao¹, WANG Fengshan³, LI Li⁴, LIU Yuhong^1,2,4*]]> REN Wenjing^1,2, MA Yan¹, CAI Meichao¹, WANG Fengshan³, LI Li⁴, LIU Yuhong^1,2,4* 2true Citrus aurantium var. amara, the essential oils from leaves, flowers and young fruits were extracted respectively by steam distillation(SD)and their chemical compositions were identified by GC-MS. Moreover, their antioxidant and antibacterial activities were evaluated and compared using in vitro methods. The results were as follows:(1)A total of 94 volatile components were isolated and identified from the essential oils of leaves, flowers and young fruits, there were some differences between three parts, of which 13 were common components. A total of 34 components were identified from leaves, and the major compounds were linalool(30.51%)and α-terpineol(14.78%); a total of 32 components were identified from flowers, the main components were linalool(57.59%)and d-limonene(16.15%); a total of 69 components were identified from young fruits, mainly containing d-limonene(25.55%)and γ-terpene(10.48%).(2)The essential oils from different parts showed different antioxidant activities. The IC₅₀ values of essential oils from young fruits, leaves and flowers to ABTS free radicals were 2.6, 5.1 and 8.2 mg·mL^-1, respectively, and the IC₅₀ values to DPPH free radicals were 2.7, 4.3 and 5.0 mg·mL^-1, respectively. The antioxidant activity of young fruits was better than that of leaves and flowers.(3)The essential oil from young fruits showed certain antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, which was better than the leaves and flowers. The results can provide a theoretical basis for the extraction and utilization of the essential oils from different parts of Citrus aurantium var. amara.]]> 2023/6/27 16:37:33 1, WEN Yali², CHEN Xiawei¹, LIN Jiaxun¹, XU Yourui^1*]]> LIU Yanan¹, WEN Yali², CHEN Xiawei¹, LIN Jiaxun¹, XU Yourui^1* 1true