ARF gene family members and their roles in response to high temperature and drought stress, the phylogenetic and expression patterns of the ARF gene family members in Huperzia serrata were analyzed using full-length transcriptome and RNA-seq data. Based on bioinformatics analysis, the physicochemical properties, domains, conserved motifs, phylogeny, tissue expression patterns, and expression patterns of the ARF gene family under high temperature and drought stress were analyzed. The results were as follows:(1)A total of 24 ARF family members were screened in the full-length transcriptome of H. serrata, all of which were acidic proteins and hydrophilic proteins.(2)Subcellular localization prediction revealed that 24 HsARF were all localized in nucleus.(3)Phylogenetic analysis revealed that HsARF had a distant genetic relationship with angiosperms Arabidopsis and rice, and only share two common ARF ancestors with higher flowering plants.(4)Domain analysis revealed that, except for HsARF18/23/24, most HsARF had B3 domains. Analysis of secondary structure found that the highest proportion of HsARF protein was random curling, followed by elongated chains and α-helix. Three-dimensional(3D)protein structure prediction model used in the 24 HsARF proteins was only four.(5)RNA-seq analysis showed that the expression levels of 7 HsARF were high in all detected organs. The expression levels of 10 HsARF in stems were higher than those in roots and leaves. Otherwise, the expression levels of HsARF13 and HsARF14 in leaves were lower than those in roots and stems.(6)The expression levels of HsARF undergo significant changes under high temperature and drought stress, with 18 HsARF being induced to varying degrees by high temperature stress, 7 HsARF responded to drought stress, with 3 HsARF induced by drought, while 4 HsARF inhibited by drought. The results provide theoretical references for the functional and biological breeding study of ARF gene family in H. serrata.]]> 2024/7/4 22:07:05 1, SHI Jidong², ZHANG Kai¹, XIE Libo¹, HUA Yangguang³, CAO Yu³, LI Junyi³, WANG Dekai^3*]]> LI Haibo¹, SHI Jidong², ZHANG Kai¹, XIE Libo¹, HUA Yangguang³, CAO Yu³, LI Junyi³, WANG Dekai^3* 2true COBRA gene family members of Huperzia serrata, the physicochemical properties, domains, conserved motifs, cis-acting elements, and genes expression of the family members(HsCOBRAs)were analyzed by bioinformatics techniques, based on the full-length transcriptome data of the H. serrata. The results were as follows:(1)A total of 24 HsCOBRAs family members were screened in the full-length transcriptome of H. serrata, including 9 acidic proteins, 11 stabilizing proteins, 5 hydrophobic proteins, 7 proteins with transmembrane structures, and 3 proteins with signal peptides.(2) Subcellular localization was found in the cell wall, chloroplast, nucleus, and cell membrane.(3)Structural analysis revealed that HsCOBRAs had 7 domains and 6 conserved motifs, and partial members had a highly conserved CCVS structure.(4)HsCOBRAs had 45 cis-acting elements such as CAAT-box and TATA-box.(5)HsCOBRA2 had the highest expressions in leaves, spores, stems and gemma. The study results can provide theoretical basis for further research and biological function verification of HsCOBRAs.]]> 2024/7/4 22:07:05 *, LIANG Liuguan]]> HUANG Yumei, TENG Jianbei, TU Dongping^*, LIANG Liuguan 1true