| 引用本文: | 罗明楚, 石小翠, 孙 朋, 鲁建美, 宋兴震,
贾慧珍, 吴 敏, 许又凯.拔毒散的化学成分及其抗炎活性研究[J].广西植物,2022,42(9):1522-1530.[点击复制] |
| LUO Mingchu, SHI Xiaocui, SUN Peng, LU Jianmei, SONG Xingzhen,
JIA Hiuzhen, WU Min, XU Youkai.Chemical constituents of Sida szechuensis and their anti-inflammatory activities[J].Guihaia,2022,42(9):1522-1530.[点击复制] |
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| 拔毒散的化学成分及其抗炎活性研究 |
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罗明楚1,2, 石小翠1,2, 孙 朋1,2, 鲁建美1,2, 宋兴震1,2,
贾慧珍1,2, 吴 敏1,2, 许又凯1*
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1. 热带植物资源可持续利用重点实验室 中国科学院西双版纳热带植物园,
云南 勐腊 666303;2. 中国科学院大学, 北京 100049
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| 摘要: |
| 拔毒散(Sida szechuensis)为一种傣族传统的药用植物,常用于治疗诸疮肿毒、皮肤瘙痒、跌打损伤、刀枪伤等疾病。为研究拔毒散的化学成分及其体外抗炎活性,该研究采用硅胶柱色谱、Sephadex LH-20柱色谱、半制备HPLC等方法对其地上部分的化学成分进行分离纯化,通过理化性质和NMR等波谱数据鉴定化合物的结构,并用脂多糖(LPS)诱导的巨噬细胞(RAW 264.7)作为炎症模型,评价所分离化合物的抗炎活性。结果表明:从拔毒散乙醇提取物中共分离得到16个化合物,分别鉴定为kaempferol-3-O-β-D-glucopyranoside(1)、 kaempferol-3-O-rutinoside(2)、槲皮素(3)、20-hydroxyecdysone(4)、α-ecdysone(5)、22-deoxyecdysterone(6)、abutasterone(7)、pterosterone(8)、icariside E5(9)、icariside E3(10)、(+)-syringaresinol(11)、pinoresinol(12)、balanophonin B(13)、N-trans-feruloyl tyramine(14)、(-)-loliolide(15)、棕榈酸(16),其中化合物1-3、9-13、16首次从该植物中分离得到。抗炎活性测试结果表明,在50 μmol·L-1的浓度下,化合物1、4、5无明显抑制NO生成作用,而化合物2、3、6-16均能在不同程度上抑制NO生成,其中化合物2、3、11-14具有较强的抗炎活性,其IC50值分别为18.63、40.76、21.46、14.32、16.82、42.31 μmol·L-1。该研究结果丰富了拔毒散的化学成分,明确了其具有抗炎效果的物质基础,验证了其传统用途的科学性,为其进一步在医药领域的开发利用提供了新思路及科学依据。 |
| 关键词: 傣药, 拔毒散, 化学成分, 分离鉴定, 抗炎活性 |
| DOI：10.11931/guihaia.gxzw202103077 |
| 分类号:Q946 |
| 文章编号:1000-3142(2022)09-1522-09 |
| 基金项目:中国科学院西双版纳热带植物园“十三五”项目(XTBG-F02); 中国科学院专项中科院合作局一带一路民族药研究与产业合作( Y6ZK131B01)[Supported by the 13th Five-Year Project of Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences(XTBG-F02); One Belt and One Road Ethnic Medicine Research and Industry Cooperation of the Cooperation Bureau of Chinese Academy of Sciences(Y6ZK131B01)]。 |
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| Chemical constituents of Sida szechuensis and their anti-inflammatory activities |
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LUO Mingchu1,2, SHI Xiaocui1,2, SUN Peng1,2, LU Jianmei1,2, SONG Xingzhen1,2,
JIA Hiuzhen1,2, WU Min1,2, XU Youkai1*
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1. Key Laboratory of Tropical Plant Resources Sciences and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy
of Sciences, Mengla 666303, Yunnan, China;2. University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| Sida szechuensis is a traditional medicine used by Dai people to treat various diseases such as furuncle, pruritus, bruise and wounds, in Xishuangbanna, Southwest, China. The chemical constituents from the aerial parts of S. szechuensis and their anti-inflammatory activities in vitro was investigated in this study. Multiple techniques, such as column chromatography on silica gel, Sephadex LH-20, semi-preparative HPLC, were used to isolate and purify compounds. Their structures were identified by physicochemical properties and NMR spectral data. All isolated compounds were evaluated for their nitric oxide(NO)inhibitory effects in lipopolysaccharide(LPS)-induced RAW 264.7 cells. The results were as follows: A total of 16 compounds were isolated from the ethanol extract and identified as kaempferol-3-O-β-D-glucopyranoside(1), kaempferol-3-O-rutinoside(2), quercetin(3), 20-hydroxyecdysone(4), α-ecdysone(5), 22-deoxyecdysterone(6), abutasterone(7), pterosterone(8), icariside E5 (9), icariside E3 (10),(+)-syringaresinol(11), pinringaresinol(12), balanophonin B(13), N-trans-feruloyl tyramine(14),(-)-loliolide(15), and palmitic acid(16). Compounds 1-3, 9-13, and 16 were isolated from this plant for the first time. The anti-inflammatory results showed that except for compounds 1, 4 and 5, all other tested compounds had NO inhibitory activities at the concentration of 50 μmol·L-1. Especially, compounds 2, 3, 11-14 demonstrated significant anti-inflammatory activities, with fifty percent NO inhibition concentration(IC50)at 18.63, 40.76, 21.46, 14.32, 16.82, 42.31 μmol·L-1, respectively. The results enrich the chemical constituents of S. szechuensis, clary the material basis of its anti-inflammatory effect, verify the scientificity of its traditional usage, and provide new insight and scientific evidence for its further development and utilization in medicine industry. |
| Key words: Dai medicine, Sida szechuensis, chemical constituents, isolation and identification, anti-inflammatory activity |
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