摘要: |
TCP是植物特有的一类转录因子,在植物生长发育过程中发挥着重要作用。该研究利用生物信息学方法对苦荞TCP家族进行全基因组鉴定,并通过实时荧光定量PCR(qRT-PCR)技术分析苦荞TCP基因在干旱胁迫和盐胁迫下的表达特征,为苦荞TCP家族的功能研究和利用奠定基础。结果表明:(1)在苦荞的基因组中鉴定出了28个TCP家族成员,不均匀地分布在苦荞的8条染色体上。(2)多数的苦荞TCP基因包含1-5个外显子。(3)系统发育分析将苦荞TCP家族分为了5个亚家族,种内TCP蛋白多聚集在同一分支上。(4)共线性分析表明,有5个苦荞TCP基因来自于全基因组复制事件。(5)顺式元件分析显示,苦荞TCP基因的启动子区域的顺式响应元件主要包含胁迫响应元件和激素响应元件两大类。(6)转录组数据分析结果显示,所有苦荞TCP基因在检测组织中均有表达(7)qPCR结果显示,FtTCP3、FtTCP6、FtTCP12和FtTCP13基因在干旱和盐胁迫下表达量发生变化,其中FtTCP3在6 h干旱处理和盐处理时表达量均达到峰值,说明FtTCP3基因在苦荞应对干旱胁迫和盐胁迫中起正向调控的作用。该研究为理解TCP基因家族的进化和功能提供了新的见解。 |
关键词: 苦荞 TCP 全基因组鉴定 非生物胁迫;实时荧光定量PCR |
DOI: |
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Fund project:国家自然科学(31671755) |
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Genome-Wide identification and abiotic stress response analysis of TCP transcription factor gene family in Tartary buckwheat(Fagopyrum tataricum) |
YANG Lanfeng1, ZHU Xudong1, ZHOU Binhan1, LUO Yirou1, LI Minghui2, FANG Zhengwu1
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1.YANGTZE UNIVERSITY;2.Agricultural Industry Service Center for Tujia Autonomous County
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Abstract: |
TCP transcription factors are a plant-specific group of transcription factors that play crucial roles in plant growth and development. In this study, bioinformatics methods were used to identified the complete genome of the Tartary buckwheat TCP gene family, and real-time quantitative PCR (qRT-PCR) analysis was conducted to investigate the expression characteristics of TCP genes under drought and salt stress. These finding provides a foundation for the functional exploration and utilization of the Tartary buckwheat TCP gene family. The results were as follows: (1) A total of 28 TCP genes were identified in the Tartary buckwheat genome, unevenly distributed across its eight chromosomes. (2) Most buckwheat TCP genes contained 1-5 exons. (3) Phylogenetic analysis classified the buckwheat TCP gene family into five clades, with intraspecific TCP proteins mainly clustering together. (4) Collinearity analysis indicated that, the five Tartary buckwheat TCP genes originated from genome-wide replication events. (5) Cis-element analysis revealed that the promoter regions of Tartary buckwheat TCP genes predominantly contained two types of cis-response elements: stress response elements and hormone response elements. (6) Transcriptomic data analysis demonstrated that all Tartary buckwheat TCP genes were expressed in the examined tissues. (7) qRT-PCR results indicated that the expression levels of FtTCP3, FtTCP6, FtTCP12, and FtTCP13 changed under drought and salt stress conditions, with FtTCP3 peaking at 6 hours of drought and salt treatments, suggesting that it play a positive regulatory role in Tartary buckwheat's response to drought and salt stress. This study provides new insights into the evolution and function of the TCP gene family. |
Key words: Tartary buckwheat, TCP transcription factors, Genome-wide identification, Abiotic stress, real-time PCR |