蛇含委陵菜的三萜类成分及其细胞毒活性研究

Triterpenoids from Potentilla kleiniana and their cytotoxicity

关键词:  蔷薇科，蛇含委陵菜，化学成分，结构鉴定，三萜，细胞毒

Key words:  Rosaceae, Potentilla kleiniana, chemical constituents, structural identification, triterpenoids, cytotoxicity

摘要:

为研究蛇含委陵菜（Potentilla kleiniana）的化学成分及其肿瘤细胞毒活性，该研究采用D-101大孔树脂、硅胶、ODS、Toyopearl HW-40、Sephadex LH-20等柱层析方法对蛇含委陵菜60%乙醇提取物进行分离纯化，结合理化性质和波谱数据鉴定化合物的结构，并运用MTT法测试各化合物对人宫颈癌细胞株Hela的细胞毒活性。结果表明：（1）从蛇含委陵菜中分离鉴定了15个三萜类化合物，分别为乌苏酸（1）、3β-O-乙酰基坡模醇酸（2）、2-氧代坡模醇酸（3）、坡模醇酸（4）、覆盆子酸（5）、野椿酸（6）、委陵菜酸（7）、 2α-羟基乌苏酸（8）、野蔷薇苷（9）、积雪草苷A（10）、常春藤皂苷元（11）、山楂酸（12）、阿江榄仁酸（13）、甘草次酸（14）、甘草酸（15）。其中，化合物 10、11、13-15为首次从委陵菜属植物中分离得到，化合物 2-5、9、12为首次从蛇含委陵菜中分离得到。（2）细胞毒试验结果显示，化合物1、2、4、6、12对人宫颈癌细胞株Hela具有一定的抑制作用，其半数抑制浓度（half maximal inhibitory concentration, IC50）分别为34.14、86.43、14.05、34.45、28.76 μmol·L-1。该研究进一步丰富了蛇含委陵菜的化学成分，为抗宫颈癌药物的研发提供了实验依据。

Abstract:

Potentilla kleiniana belongs to the family Rosaceae, which distributes in Central Asia, East Asia and Southeast Asia. In China, this plant is mainly found in east, south and southwest provinces. P. kleiniana has been prescribed for the treatment of various diseases in the field of traditional Chinese medicine, such as cough, fever, tuberculosis, mastitis, rheumatoid arthritis. Our previous study found that P. kleiniana had a certain cytotoxicity on tumor cells, and 28 compounds were isolated and identified from P. kleiniana, some of which were cytotoxic to tumor cells. In order to further study the chemical constituents of Potentilla kleiniana and their cytotoxicity, the 60% ethanol extract of P. kleiniana were isolated by D-101 macroporous adsorptive resins, silica gel, ODS, Toyopearl HW-40, Sephadex LH-20 and other methods. Their chemical structures were elucidated on the basis of physicochemical properties and spectroscopic data. Meanwhile, all these compounds were evaluated for cytotoxic activities against human cervical cancer cell line Hela. The results were as follows: (1) Fifteen triterpenoids were isolated and identified as ursolic acid (1), 3β-O-acetyl pomolic acid (2), 2-oxopomolic acid (3), pomolic acid (4), fupenzic acid (5), euscaphic acid (6), tormentic acid (7), 2α-hydroxyursolic acid (8), rosamultin (9), asiaticoside A (10), hederagenin (11), maslinic acid (12), arjunic acid (13), glycyrrhetinic acid (14), glycyrrhizic acid (15). Among them, compounds 10, 11, 13-15 were isolated from genus Potentilla for the first time, and compounds 2-5, 9 and 12 were isolated from P. kleiniana for the first time. (2) Cytotoxic activity studies showed that compounds 1, 2, 4, 6, 12 display certain inhibitory activities against Hela cells with half maximal inhibitory concentration (IC50) values of 34.14, 86.43, 14.05, 34.45, 28.76 μmol·L-1, respectively. Thus, the study enriches the chemical constituents of P. kleiniana, and provides experimental basis for the development of anti-cervical cancer drugs.