桃花芽休眠期PpPP2C基因鉴定与表达分析

李海炎<sup>1</sup>; 朱鹏翔<sup>1</sup>; 漓 君<sup>2</sup>; 陆泰良<sup>1</sup>; 许京华<sup>2</sup>; 万保雄<sup>1*</sup>

引用本文:	李海炎, 朱鹏翔, 漓君, 陆泰良, 许京华, 万保雄.桃花芽休眠期PpPP2C基因鉴定与表达分析[J].广西植物,2026,46(8):1294-1302.[点击复制]
	LI Haiyan, ZHU Pengxiang, LI Jun, LU Tailiang, XU Jinghua, WAN Baoxiong.Identification and expression analysis of PpPP2C genes in the dormant period of peach flower buds[J].Guihaia,2026,46(8):1294-1302.[点击复制]

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*桃花芽休眠期PpPP2C基因鉴定与表达分析*
李海炎¹, 朱鹏翔¹, 漓君², 陆泰良¹, 许京华², 万保雄^1*
1. 广西桂北特色经济作物种质创新与利用实验室/广西特色作物研究院, 广西桂林 541004;2. 灵川县水果生产技术服务中心, 广西桂林 541200

摘要:

为探明桃(Prunus persica)PP2C基因家族成员在桃花芽休眠期间的表达特性,该研究从休眠期桃花芽转录组数据中鉴定出7个PpPP2C基因,利用生物信息学方法分析其理化性质、系统进化关系、保守基序及启动子顺式作用元件等,并采用qRT-PCR方法检测不同需冷量(chilling requirement,CR)桃花芽PpPP2C基因从休眠前至休眠解除的表达情况。结果表明:(1)7个PpPP2C基因编码氨基酸序列的理化性质和保守基序结构具有较高相似性。7个PpPP2C均属于A亚族,而A亚族是脱落酸(ABA)信号通路的关键因子,说明在桃花芽休眠过程中PpPP2C是通过ABA信号转导过程参与休眠调控。(2)PpPP2C启动子区域存在丰富的脱落酸响应元件、光响应元件和茉莉酸甲酯响应元件,推测这3类元件能够调控PpPP2C基因转录进而调控休眠。(3)qRT-PCR结果显示,PpPP2C基因在进入休眠前表达量较高,进入休眠后表达量降低,说明休眠会抑制PpPP2C的表达; 而CR≥400 h品种PpPP2C基因表达量比CR≤200 h品种更早开始降低,这种差别可能是由需冷量不同导致进入休眠的时期不一致,CR≤200 h品种需要更短的日照或低温从而延迟进入休眠。该研究初步阐明了PP2C 基因在ABA调控休眠中的作用,为后续深入解析其调控网络提供了重要依据。

关键词: 桃, 休眠, PpPP2C, 生物信息学, 表达特性

DOI：10.11931/guihaia.gxzw202509030

分类号:Q945

文章编号:1000-3142(2026)08-1294-09

基金项目:广西科技计划项目(桂科AD23026003); 广西自然科学基金面上项目(2025GXNSFAA069802); 广西农业和乡村振兴人才培养支持专项(GXQNTJ242130); 广西特色作物试验站项目(桂TS202108)。

Identification and expression analysis of PpPP2C genes in the dormant period of peach flower buds

LI Haiyan¹, ZHU Pengxiang¹, LI Jun², LU Tailiang¹, XU Jinghua², WAN Baoxiong^1*

1. Guangxi Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi /Guangxi Academy of Specialty Crops, Guilin 541004, Guangxi, China;2. Lingchuan County Fruit Production Technology Service Center, Guilin 541200, Guangxi, China

Abstract:

To characterize the expression of PP2C gene family members during peach flower bud dormancy, seven PpPP2C genes were identified from transcriptome data of dormant peach flower buds. Bioinformatics methods were used to analyze the physicochemical properties, phylogenetic relationships, conserved motifs, and promoter cis-acting elements, and qRT-PCR was employed to measure PpPP2C expression levels in varieties with differing chilling requirements from pre-dormancy to dormancy release. The results were as follows:(1)The amino acid sequences encoded by the seven PpPP2C genes exhibited highly similar physicochemical properties and conserved motif structures. All seven PpPP2C genes belonged to Subfamily A, a key component of the abscisic acid(ABA)signaling pathway, suggesting their involvement in dormancy regulation through ABA signal transduction.(2)The promoter regions of PpPP2C genes contained abundant abscisic acid, light, and methyl jasminate response elements, which are thought to regulate PP2C transcription during dormancy.(3)qRT-PCR results showed higher PpPP2C expression before dormancy, with levels declining after dormancy onset, indicating that dormancy suppressed PpPP2C expression. However, in varieties with CR≥400 h, this decrease began earlier than in those with CR≤200 h. This discrepancy might be attributed to different timings of dormancy entry resulting from varying chilling requirements, where varieties with CR≤200 h required shorter day lengths or lower temperature and consequently entered dormancy later. This study preliminarily elucidated the role of PP2C genes in ABA-mediated dormancy regulation, providing an important basis for further analysis of its regulatory network.

Key words: peach, dormant, PpPP2C, bioinformatics, expression characteristics