| 摘要: |
| 为挖掘普通小麦(Triticum aestivum)抗逆关键基因,该研究结合生物信息学方法,对普通小麦DUF292基因家族进行全基因组鉴定及功能分析。结果表明:(1)在普通小麦中共鉴定出33个TaDUF292成员,命名为TaDUF292-01-TaDUF292-33,非均匀分布于15条染色体。(2)该家族蛋白均为亲水蛋白,主要定位于叶绿体、线粒体及细胞核。(3)系统发育分析将其分为3个进化分支,分支内基因结构与保守基序具有一致性。(4)启动子顺式作用元件以光响应及激素调控元件为主,提示该基因家族参与环境信号整合。(5)miRNA靶向预测显示,16类切割抑制型和5类翻译抑制型miRNA构成调控网络。(6)表达模式分析显示,TaDUF292家族成员呈现组织特异性表达,且对干旱、盐、高温及病原菌侵染的响应存在差异,部分成员在复合胁迫下显著上调。该研究系统揭示了小麦TaDUF292家族的分子特征与潜在功能,为普通小麦抗逆分子育种提供了基因资源和理论依据。 |
| 关键词: 小麦, DUF292, 基因家族, 表达模式, 生物信息学 |
| DOI：10.11931/guihaia.gxzw202601021 |
| 分类号:Q943 |
| 文章编号:1000-3142(2026)08-1339-14 |
| 基金项目:中国成人教育协会一般课题(2024-SJYB-098S)。 |
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| Identification and expression pattern analysis of the DUF292 gene family in wheat |
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MA Xiaoru1, LIU Jie2, LIANG Jing1*
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1. School of Economics and Management, Open University of Xinjiang, Urumqi 830001, China;2. Shihezi University, Shihezi 832003, Xinjiang, China
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| Abstract: |
| To mine key stress-resistant genes in common wheat(Triticum aestivum), this study performed genome-wide identification and functional analysis of TaDUF292 gene family using bioinformatics approaches. The results were as follows:(1)A total of 33 TaDUF292 members were identified in common wheat and designated TaDUF292-01 to TaDUF292-33, which distributed unevenly on 15 chromosomes.(2)All proteins encoded by the TaDUF292 family were hydrophilic proteins and were mainly localized in chloroplasts, mitochondria and nuclei.(3)Phylogenetic analysis divided the TaDUF292 family into three evolutionary clades, and genes in the same clade exhibited consistent gene structures and conserved motifs.(4)Cis-acting elements in promoters were dominated by light-responsive and hormone regulatory elements, which indicated that this gene family participated in the integration of plant environmental signals.(5)miRNA target prediction revealed that 16 types of cleavage-inhibiting miRNAs and 5 types of translation-inhibiting miRNAs jointly constituted a regulatory network.(6)Expression pattern analysis showed that TaDUF292 family genes exhibited obvious tissue-specific expression and differential response patterns to drought, salt, heat and pathogen infection stresses, and several members were significantly upregulated under combined stress conditions. This study systematically clarifies the molecular characteristics and potential function of the TaDUF292 family in common wheat, providing genetic resources and a theoretical basis for stress-resistant molecular breeding of common wheat. |
| Key words: wheat, DUF292, gene family, expression patterns, bioinformatics |
