| 引用本文: | 杨 敏, 谭 晶, 杨孙缘, 熊荣琴, 曾艳荣, 张 振, 路 浩, 谭承建.黄花棘豆中1个新的黄酮苷化合物及生物活性研究[J].广西植物,2026,46(8):1385-1393.[点击复制] |
| YANG Min, TAN Jing, YANG Sunyuan, XIONG Rongqin, ZENG Yanrong,
ZHANG Zhen, LU Hao, TAN Chengjian.A new flavonoid glycoside from Oxytropis ochrocephala and the biological activities[J].Guihaia,2026,46(8):1385-1393.[点击复制] |
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| 黄花棘豆中1个新的黄酮苷化合物及生物活性研究 |
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杨 敏1, 谭 晶1, 杨孙缘1, 熊荣琴1, 曾艳荣1, 张 振2, 路 浩3, 谭承建1*
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1. 贵州民族大学 民族医药学院, 贵阳 550025;2. 贵州科学院, 贵阳 550025;3. 西北农林科技大学 动物医学院, 陕西 杨凌 712100
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| 摘要: |
| 为研究黄花棘豆的化学成分及其抗氧化活性,该研究采用硅胶色谱柱、Sephadex LH-20色谱柱及半制备高效液相色谱等色谱技术对黄花棘豆醇提物进行分离纯化,并综合运用UV、IR、HR-MS、NMR等波谱学方法鉴定其化合物结构,以及通过1,1-二苯基-2-三硝基苯肼(DPPH)和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)(ABTS)的自由基清除实验评价化合物的抗氧化活性。结果表明:(1)从黄花棘豆中分离鉴定出12个黄酮类成分,分别为ochrocephalamine Q(1)、(6aR,11aR)-10-hydroxy-3,9-dimethoxypterocarpan(2)、3',7-二羟基-2',4'-二甲氧基异黄烷(3)、bosenegaloside A(4)、5-hydroxy-2-(4-hydroxyphenyl)-7-methoxy-3- [6-O-(1-oxohexadecyl)-α-D-altropyranosyl]-4H-1-benzopyran-4-one(5)、鼠李柠檬素(6)、3,5,7-三羟基-4'-甲氧基黄酮醇(7)、3-O-鼠李柠檬素-6-O-苯甲酰基-β-D-吡喃葡萄糖苷(8)、鼠李柠檬素-3-O-β-D-半乳糖苷(9)、异槲皮苷(10)、鼠李素-3-O-β-D-半乳糖苷(11)及鼠李柠檬素-3-O-β-D-葡萄糖苷(12)。其中,化合物1为新化合物,化合物2、4、5为首次从黄花棘豆中分离得到。(2)化合物6、10、11在DPPH自由基清除实验中表现出较强的抗氧化活性,其IC50值分别为(4.90±0.35)、(0.80±0.06)、(1.07±0.02)μg·mL-1,强于阳性对照维生素C [IC50值为(5.72±0.52)μg·mL-1]。同时,在ABTS自由基清除实验中,化合物11亦表现出较强的抗氧化活性,其IC50值为(0.32±0.05)μg·mL-1。该研究结果进一步丰富了黄花棘豆的化学成分,为其资源化利用提供了研究资料。 |
| 关键词: 黄花棘豆, 黄酮, 化学成分, 自由基清除, 抗氧化 |
| DOI：10.11931/guihaia.gxzw202511014 |
| 分类号:Q946 |
| 文章编号:1000-3142(2026)08-1385-09 |
| 基金项目:国家自然科学基金项目(32160110,31660103); 贵州省研究生教育创新计划项目(2024YJSKYJJ225)。 |
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| A new flavonoid glycoside from Oxytropis ochrocephala and the biological activities |
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YANG Min1, TAN Jing1, YANG Sunyuan1, XIONG Rongqin1, ZENG Yanrong1,
ZHANG Zhen2, LU Hao3, TAN Chengjian1*
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1. School of Ethnic-Minority Medicine, Guizhou Minzu University, Guiyang 550025, China;2. Guizhou Academy of Sciences, Guiyang
550025, China;3. College of Veterinary Medicine, Northwest A &4.F University, Yangling 712100, Shaanxi, China
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| Abstract: |
| To investigate the chemical constituents of Oxytropis ochrocephala and their antioxidant activities, the ethanol extract of O. ochrocephala was separated and purified using chromatographic techniques including silica gel column chromatography, Sephadex LH-20 chromatography and semi-preparative high-performance liquid chromatography. The structures of the isolated compounds were elucidated by comprehensive spectroscopic analyses, including UV, IR, HR-MS and NMR. The antioxidant activities of the isolated compounds were evaluated using 1, 1-diphenyl-2-picrylhydrazyl(DPPH)and 2, 2-azinobis-(3-ethyl-benzothiazole-6-sulfonic acid)(ABTS)radical scavenging assays. The results were as follows:(1)Twelve compounds were isolated and identified as ochrocephalamine Q(1),(6aR, 11aR)-10-hydroxy-3, 9-dimethoxypterocarpan(2), 3', 7-dihydroxy-2', 4'-dimethoxyisoflavan(3), bosenegaloside A(4), 5-hydroxy-2-(4-hydroxyphenyl)-7-methoxy-3-[6-O-(1-oxohexadecyl)-α-D-altropyranosyl]-4H-1-benzopyran-4-one(5), rhamnocitrin(6), 3, 5, 7-trihydroxy-4'-methoxyflavonol(7), 3-O-rhamnocitrin-6-O-benzoyl-β-D-glucopyranoside(8), rhamnocitrin-3-O-β-D-galactoside(9), isoquercitrin(10), rhamnetin 3-O-β-D-galactoside(11)and rhamnocitrin 3-O-β-D-glucoside(12). Among these, Compound 1 was a new compound, while compounds 2, 4 and 5 were reported in this genus for the first time.(2)Compounds 6, 10 and 11 exhibited notable antioxidant activities in the DPPH radical scavenging assay, with IC50 values of(4.90±0.35),(0.80±0.06)and(1.07±0.02)μg·mL-1, respectively, which were stronger than that of the positive control vitamin C [IC50 value of(5.72±0.52)μg·mL-1]. In addition, in the ABTS radical scavenging assay, Compound 11 also showed strong antioxidant activity, with IC50 values of(0.32±0.05)μg·mL-1. These findings further enrich the chemical constituents of O. ochrocephala and provide valuable information for its potential resource development and utilization. |
| Key words: Oxytropis ochrocephala, flavonoids, chemical constituents, free radical scavenging, antioxidant |
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