红根草的化学成分及其抗氧化活性研究

Chemical constituents from Salvia prionitis and their antioxidant activities

关键词:  红根草, 化学成分, 分离鉴定, 萜类化合物, 抗氧化活性

Key words:  Salvia prionitis, chemical constituents, separation and identification, terpenoids, antioxidant activity

摘要:

为探究红根草(Salvia prionitis)的化学成分及其抗氧化活性,该研究采用MCI、硅胶、ODS、HW-40F及高效液相色谱(HPLC)对红根草乙酸乙酯部位进行分离纯化,并且综合运用波谱学方法(1D/2D-NMR、HR-MS、CD)及化学计算鉴定化合物结构,通过1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除法评价化合物的抗氧化能力。结果表明:(1)从红根草中分离得到17个化合物,分别鉴定为3-hydroxysalvinolone(1)、丹参酚酮(2)、salviadesertin J(3)、5α,11,12-trihydroxy-6-oxaabieta-8,11,13-trien-7-one(4)、桧醇(5)、perovskin C(6)、4-羟基红根草对醌(7)、prineoparaquinone(8)、miltiolactone(9)、2α,3α-dihydroxyursolic acid 28-O-β-D-glucopyranosyl ester(10)、19α-羟基积雪草酸(11)、齐墩果酸(12)、橙皮苷(13)、异槲皮苷(14)、芦丁(15)、山奈酚-3-O-β-D-葡萄糖苷(16)和山奈酚-3-O-芸香糖苷(17)。其中,化合物1为新化合物,化合物4、6、10、11、13和17为首次从鼠尾草属植物中分离得到,化合物3和9为首次从该植物中分离得到。(2)DPPH自由基清除测试结果表明,化合物14[IC₅₀ =(0.009 2±0.000 06)mmol·L^-1]和15[IC₅₀=(0.013 2±0.000 06)mmol·L^-1]的活性强于阳性对照[抗坏血酸,IC₅₀ =(0.044 2±0.000 46)mmol·L^-1]。该研究结果可为红根草抗氧化相关产品的开发提供理论依据。

Abstract:

To study the constituents from Salvia prionitis and their antioxidant activities. The ethyl acetate fraction of S. prionitis was separated and purified by a combination of column using MCI, silica gel, ODS, HW-40F, and high performance liquid chromatography(HPLC). The structures of obtained compounds were determined on the basis of spectroscopic data(1D-/2D-NMR, HR-MS, CD)and chemical calculations. The antioxidant capacities were evaluated using the1, 1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging assay. The results were as follows:(1)Seventeen compounds identified as 3-hydroxysalvinolone(1), salvinolone(2), salviadesertin J(3), 5α,11,12-trihydroxy-6-oxaabieta-8,11,13-trien-7-one(4), hinokiol(5), perovskin C(6), 4-hydroxysapriparaquinone(7), prineoparaquinone(8), miltiolactone(9), 2α,3α-dihydroxyursolic acid 28-O-β-D-glucopyranosyl ester(10), 19α-hydroxyasiatic acid(11), oleanolic acid(12), hesperidin(13), isoquercitrin(14), rutin(15), kaempferol-3-O-β-D-glucoside(16), and kaempferol-3-O-rutinoside(17). Compound 1 was identified as a new compound. The compounds 4, 6, 10, 11, 13, and 17 were the first time isolated from the genus Salvia plants, while compounds 3 and 9 were the first time obtained from S. prionitis.(2)The results of the DPPH free radical scavenging assay showed that compounds 14 [IC₅₀=(0.009 2±0.000 06)mmol·L^-1] and 15 [IC₅₀=(0.013 2±0.000 06)mmol·L^-1)exhibited stronger antioxidant activities than the positive control [ascorbic acid, IC₅₀=(0.044 2±0.000 46)mmol·L^-1]. This study provides a theoretical basis for developing novel derivatives from S. prionitis with enhanced antioxidant activity, guiding future product discovery efforts.