摘要: |
糯高粱是白酒酿造的重要原料,其生长过程需要大量的化学肥料,为开发对糯高粱有促进生长功能的微生物菌剂,本研究从糯高粱叶片中分离筛选具有植物生长激素IAA(吲哚乙酸)产生能力的微生物菌株,并分析其对糯高粱种子发芽和高粱幼苗生长的影响。结果表明:(1)从糯高粱叶片中分离筛选得到4株具有产IAA能力的微生物菌株,HY1-1、HY1-2、HY1-3和HY1-4。其中IAA单位浓度产生量最高的是菌株HY1-1,为2.552 μmol/mL。(2)运用贝叶斯推断树对菌株16S rDNA进行系统发育分析,这4株菌都属于枯草芽孢杆菌Bacillus subtilis。(3)HY1-1、HY1-2、HY1-3和HY1-4都能促进糯高粱种子萌发,与对照组相比,经菌悬液浸泡的糯高粱种子发芽率显著提高了40.04%~165.52%,其中促进效果最明显的是HY1-1,种子发芽率提高了165.52%。(4)选取HY1-1菌株做盆栽实验,在糯高粱幼苗根部接种B. subtilis HY1-1 30天后,糯高粱幼苗的株高显著增加了29.17%、全磷含量显著增加了5.12%;糯高粱根际基质中速效氮显著增加了31.7%,有效磷显著增加了28.88%。糯高粱叶内生菌B. subtilis HY1-1能够通过分泌植物生长激素IAA及为植物提供营养元素等方式促进糯高粱植株的生长,该研究为进一步开发促糯高粱生长菌剂提供种质资源。 |
关键词: 植物内生菌,分离筛选,植物生长素IAA,种子萌发,促高粱生长 |
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基金项目:贵州省科技厅基础研究计划项目(黔科合基础【2020】1Y076),贵州省教育厅科技拔尖才项目(黔教合KY字【2020】035),贵州省教育厅青年科技人才成长项目(黔教合KY字【2020】232),遵义市科学技术局——茅台学院市校联合科技研发资金项目(遵市科合HZ字【2020】317号、遵市科合HZ字【2021】326号、遵市科合HZ字【2021】312号、遵市科合HZ字【2021】307号),茅台学院高层次人才科研启动项目(mygccrc【2022】023、mygccrc【2022】027) |
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Isolation and screening of IAA-producing bacteria from glutinous sorghum leaves and its growth-promoting function |
王新叶, Zhao Liang
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Abstract: |
Glinous sorghum is an important raw material for the production of white wine. Its growth process requires a substantial amount of chemical fertilizers. In order to develop microbial agents that promote the growth of glutinous sorghum, this study isolated and screened microbial strains with the capability to produce the plant growth hormone IAA from glutinous sorghum leaves. The effects of these microbial strains on glutinous sorghum seed germination and seedling growth were analyzed. The results were as follows: (1) Four microbial strains capable of producing IAA were isolated and screened from glutinous sorghum leaves, designated as HY1-1, HY1-2, HY1-3, and HY1-4. Among them, HY1-1 exhibited the highest IAA production per unit concentration which was 2.552 μmol/mL. (2) Bayesian inference tree analysis based on the 16S rDNA sequences revealed that all four strains belonged to Bacillus subtilis. (3) HY1-1, HY1-2, HY1-3, and HY1-4 could promote the glutinous sorghum seed germination rates. Compared to the control groups, the germination rates of glutinous sorghum seeds soaked in bacterial suspension significantly increased by 40.04% to 165.52%. Among them, HY1-1 demonstrated the most prominent promotion effect, with a germination rate increase of 165.52%. (4) HY1-1 strain was selected for a pot experiment. After 30 days of inoculation on the roots of glutinous sorghum seedlings, the stem heights of the seedlings significantly increased by 29.17%, and total phosphorus content increased significantly by 5.12%. The rhizosphere substrate of glutinous sorghum exhibited a significant increase in available nitrogen content by 31.7% and available phosphorus content by 28.88%. B. subtilis HY1-1, an endophytic bacterium in glutinous sorghum leaves, can promote the growth of glutinous sorghum plants by secreting the plant growth hormone IAA and providing necessary nutrients. This study provides genetic resources for the further development of microbial agents targeting the growth promotion of glutinous sorghum. |
Key words: plant endophyte bacteria plant hormone IAA production seed germination glutinous sorghum growth promoting |