Page 16 - 《广西植物》2022年第10期
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1 6 3 4                                广  西  植  物                                         42 卷
                    Table 1  Species namesꎬ localitiesꎬ vocher information and GenBank accession
                                 numbers of DNA sequences used in this study

    Taxon                                     Locality                 Voucher specimen       rbcL

    Isoetes flaccida Shuttlew. ex A. Braun      —                    Abbott 20265 (FLAS)    KJ773600
    Selaginella braunii Baker               Cultꎬ Thailand             Tiew 12 (CDBI)       KT161421
    S. bryopteris (L.) Baker                   Nepal              C. R. Fraser ̄Jenkins 4370 (L)  KY022983
    S. ciliaris Spring                     Yunnanꎬ China            X. C. Zhang 7780 (PE)  MH814892
    S. convoluta (Arn.) Spring              Bahiaꎬ Brazil           R. M. Harley 16181 (U)  KY023003
    S. digitata Spring                       Madagascar           N. Wikström et al. 110319 ̄2 (S)  KY023013
    S. digitata Spring                       Madagascar              P. Phillipson 1826 (L)  KY023012
    S. exaltata (Kunze) Spring                Ecuador                 Korall 1996 ̄1 (S)     AJ010849
    S. helicoclada Alston                       —                   Rakotondrainibe 3262 (P)  AJ295896C
    S. helvetica (L.) Spring                  France                 J. C. Bertier 9161 (PE)  MW407303
    S. heterostachys Baker                 Guizhouꎬ China           X. C. Zhang 7088 (PE)  MH814896
    S. imbricata (Forssk.) Spring       Dhofar Governorateꎬ Oman   Rothfels et al. 4275 (DUKE)  KT161486
    S. kraussiana (Kunze) A. Braun      Democratic Republic of the   M. Mokoso 3098 (BR)   KY023057
                                          Congo (South Kivu)
    S. lepidophylla (Hook. & Grev.) Spring      —                           —               AF419051
    S. lutchuensis Koidz.                      Japan                  TNS 759343 (TNS)     AB574648
    S. moellendorffii Hieron.              Sichuanꎬ China         Ju & Deng HGX 12295 (CDBI)  KT161531
    S. nipponica Franch. et Sav.           Guizhouꎬ China         X. C. Zhang et al. 7066 (PE)  MW407367
    S. nothohybrida Valdespino           San Luis Potosíꎬ Mexico   C. J. Rothfels 3069 (DUKE)  KY023096
    S. novoleonensis Hieron. & Sadeb       Sonoraꎬ Mexico        F. Drouet and D. Richards 3942 (S)  KY023097
    S. nubigena J. P. Roux                  South Africa            A. Larsson AL810 (UPS)  KY023098
    S. pallescens (C. Presl) Spring          Unknown                        —               AJ295859
    S. pilifera A. Braun                        —                     Pringle 13959 (S)     AJ295862
    S. pseudotamariscina X. C. Zhang & C. W.  Vietnam              C. W. Chen Wade 5314 (PE)  MZ159980∗
      Chenꎬ sp. nov.
    S. pulvinata (Hook. & Grev.) Maxim     Sichuanꎬ China         D. E. Boufford et al. 37879 (A)  KY023124
    S. pulvinata (Hook. & Grev.) Maxim     Yunnanꎬ China          D. E. Boufford et al. 35254 (A)  KY023125
    S. remotifolia Spring                  Yunnanꎬ China            Gaoligong Shan Biodiversity  KY023130
                                                                      Survey 21081 (GH)
    S. selaginoides (L.) P. Beauv. ex Schrank & Mart.  Sweden       S. Weststrand 104 (UPS)  KY023148
    S. sibirica (Milde) Hieron.               Alaska             L. A. Viereck and K. Jones 5667 (S)  KY023153
    S. stauntoniana Spring                  Beijingꎬ China            Zhao 169 (CDBI)       KT161614
    S. tamariscina (P. Beauv.) Spring          Japan                  TNS759348 (TNS)      AB574655
    S. uliginosa (Labill.) Spring               —                 Holmgren and Wanntorp 253 (S)  AJ010843
    S. uncinata (Desv.) Spring             Sichuanꎬ China        Zhang and Zhou DJY04101 (CDBI)  KT161626
    S. vardei H. Lév.                       Tibetꎬ China          D. E. Boufford et al. 32425 (A)  KY023169
     Note: — indicates missing dataꎻ ∗ indicates newly generated sequences.

   trimethylammonium bromide ( CTAB) method ( Li et      Sequences were aligned using MUSCLE (Edgarꎬ
   al.ꎬ 2013). Library construction was performed with the  2004)ꎬ followed by manual adjustment in PhyDE
   NEBNext DNA Library Prep Kit (New England Biolabsꎬ  v0.9971 ( Muller et al.ꎬ 2010). Maximum likelihood
   Ipswichꎬ Massachusettsꎬ USA). Paired ̄end reads of 2 ×  (ML) analyses were performed using RAxML 7. 2. 6

   150 bp were generated on an Illumina HiSeq 2500   (Stamatakisꎬ 2006)ꎬ with 1 000 bootstrap replicates
   instrumentꎬ and rbcL gene was extracted.          under the GTRGAMMA model. We performed Bayesian
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