外源激素对马尾松花芽分化及内源物质的影响

Effects of exogenous hormones on flower bud differentiation and endogenous substances of Pinus massoniana

关键词:  马尾松, 外源激素, 雌雄球花分化, 内源物质

Key words:  Pinus manssoniana, exogenous hormones, male and female cones differentiation, endogenous substances

摘要:

为探讨马尾松球花形成与植物激素水平的关系,该研究对贵州省都匀无性系种子园11年生马尾松进行不同浓度的 IAA、IBA、GA₃、BAP等植物激素处理,采用考马斯亮蓝G-250染色法、蒽酮法分别对不同浓度不同激素处理后的枝条上针叶中的可溶性蛋白质和可溶性糖含量变化进行测定,并在第二年开花时对试验枝条的开花情况进行了调查。结果表明:在8-11月份,进行500 mg·L^-1 的BAP处理有利于马尾松雌球花和雄球花的形成,100 mg·L^-1 的GA₃ 处理有利于马尾松雌球花的形成; 而GA₃ 250 mg·L^-1 和GA₃ 500 mg·L^-1 处理有利于马尾松雄球花的形成,IAA 250 mg·L^-1 对马尾松雌雄球花同枝的数量有提高作用。在10-11月份,对马尾松进行500 mg·L^-1 的BAP、IAA、GA₃ 处理后,马尾松针叶内蛋白质含量变化有显著影响; 在10月份时,进行BAP 100 mg·L^-1 处理后,其可溶性糖含量及可溶性蛋白含量均可达到极显著水平。而在8月份与10月份时,分别进行IBA 100 mg·L^-1 与IBA 250 mg·L^-1 处理后,其可溶性蛋白含量与其对照差异处于极显著水平; 在11月份时,进行GA₃ 100 mg·L^-1 处理后,其可溶性蛋白含量与其对照差异处于极显著水平; 而在11月份时,进行IBA 500 mg·L^-1 处理后,其可溶性蛋白含量与对照差异处于显著水平。

Abstract:

In order to investigate the relationship between the formation of Pinus massoniana cones plant hormone levels,This paper carried out a series of hormone treatments by means of injecting IAA, IBA, GA₃, BAP, etc. to the 11 years old P. massoniana in Duyun P. massoniana Clonal Seed Orchard, and then the changes of soluble protein and the content of soluble sugar were measured under different concentrations of different hormones using Coomassie Brilliant Blue G-250 staining and anthrone method respectively. And the bloom of the test branches was investigated in the second year. The experimental results were as follows: Firstly, in August to November, the BAP with 500 mg·L^-1 was conducive to the formation of male and female cones of P. massoniana. Secondly, the GA₃ with 100 mg·L^-1 was conducive to the formation of female cones of P. massoniana. Thirdly, both GA₃ with 250 mg·L^-1 and GA₃ with 500 mg·L^-1 were conducive to the formation of male cones of P. massoniana. Fourthly, the IAA with 250 mg·L^-1 was conducive to increase the number of male and female cone with the same branch. Finally, treating the masson by injecting BAP, IAA and GA₃ with 500 mg·L^-1 respectively in October and November could have a significant impact on the changes of protein content in needles of P. massoniana. Finally, In October, using the concentration of BAP 100 mg·L^-1 of the branches to process their soluble sugar content and soluble protein content could reach a significant level. Respectively in August and October, with a concentration of 100 mg·L^-1 IBA and concentration of IBA 250 mg·L^-1 processed soluble protein content and its control was in a very significant difference, In November, using the concentration of 100 mg·L^-1 GA₃ processed soluble protein content and its control was in a very significant difference. While in November using the concentration of IBA 500 mg·L^-1, soluble protein content and the control difference were significant.