利用ISSR分子标记检测空间诱导罗汉果DNA突变

Effects of space mutation on DNA of Siraitia grosvenorii detecting by ISSR molecular markers

关键词:  罗汉果, 空间诱变育种, ISSR, 分子标记辅助选择

Key words:  Siraitia grosvenorii, space mutation breeding, ISSR, molecular marker-assisted selection

摘要:

为探究太空环境对罗汉果造成的诱变效应,筛选罗汉果新品种培育优异种质,该研究运用ISSR分子标记技术,对28个航天诱变罗汉果及主栽品种进行了全基因组多态性检测和聚类分析。结果表明:从100个ISSR引物中筛选得到17个引物,共扩增出157个条带,其中83条具有多态性,多态条带百分率为52.87%,遗传相似系数范围为0.707～0.987。根据UPGMA聚类图,28个罗汉果样本可以分为3类:聚类Ⅰ为航天种质B6 和B3♀; 聚类Ⅱ为航天种质A1♀、A14、A18 与主栽品种; 聚类Ⅲ中都为航天罗汉果种质。上述结果暗示A1♀、A14、A18 与其他航天种质已经产生了一定的遗传分化,具有与主栽品种相似的遗传背景,可能获得了有益突变。该研究结果为罗汉果新品种培育和杂交亲本选配提供了科学依据。

Abstract:

In order to investigate genetic variation of Siraitia grosvenorii after space flight, and to create elite germplasms, ISSR(inter-simple sequence repeats)molecular markers were used to detect the genome-wide DNA polymorphism and to make cluster analysis of 28 S. grosvenorii samples including space-flight accessions and main cultivars. Se-venteen primers which had clear polymorphic bands were selected from 100 primers. A total of 157 bands were detected, of which 83 bands were polymorphic with a polymorphic rate of 52.87%. Genetic similarity coefficient ranged from 0.707 to 0.987. UPGMA cluster analysis revealed that 28 samples formed three primary distinct clusters: Cluster I included two space mutation germplasms B6 and B3♀; Cluster Ⅱ contained space mutation germplasm A1♀, A14, A18 and all the main cultivars; The remaining space mutation germplasm belonged to cluster Ⅲ. These results were as follows:(1)Space mutation could result in genetic variation of S. grosvenorii.(2)A1♀, A14 and A18 might gain positive mutation because their genetic backgrounds were similar with the main cultivars, which could provide useful information for creating new elite germplasms in S. grosvenorii.