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| 翠柏二萜类成分及其抗炎活性研究 |
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丁林芬1, 吴兴德2, 王双燕3, 涂文超1, 潘争红4, 郭亚东1, 宋流东1*
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1. 昆明医科大学 药学院暨云南省天然药物药理重点实验室, 昆明 650500;2. 中国科学院昆明植物研究所 植物化学与
西部植物资源持续利用国家重点实验室, 昆明 650201;3. 云南经济管理学院 康复与护理学院, 昆明 650106;4. 广西壮族自治区
中国科学院 广西植物研究所, 广西植物功能物质研究与利用重点实验室, 广西 桂林 541006
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| 摘要: |
| 采用正相硅胶、MCI、反相RP-18和半制备液相等色谱技术, 对柏科翠柏属植物翠柏(Calocedrus macrolepis)中的二萜类成分及其抗炎活性进行了研究。结果表明:从中共分离得到8个二萜类化合物,分别鉴定为8-hydroxy-labda-13(16),14-dien-19-yl trans-coumarate(1)、trans-communal(2)、trans-communic acid(3)、pinusolidic acid(4)、isocupressic acid(5)、fokihodgin F(6)、acetylisocupressic acid(7)、15,16-dihydroxy-labda-8(17),13(E)-dien-19-oic acid(8)。化合物1～8均为首次从该植物中分离得到。化合物7对LPS诱导的RAW264.7巨噬细胞释放NO具有显著的抑制作用,其IC50值为9.31 μmol·L-1。 |
| 关键词: 柏科, 翠柏属, 翠柏, 二萜, 成分, 抗炎活性 |
| DOI：10.11931/guihaia.gxzw201604013 |
| 分类号:Q946, R284.2 |
| 文章编号:1000-3142(2017)05-0642-06 |
| Fund project:云南省天然药物药理重点实验室开放研究基金(2015G004)[Supported by Open Research Foundation of Yunnan Key Laboratory of Pharmacology for Natural Products(2015G004)]。 |
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| Diterpenoids from Calocedrus macrolepis and their anti-inflammatory activities |
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DING Lin-Fen1, WU Xing-De2, WANG Shuang-Yan3, TU Wen-Chao1,
PAN Zheng-Hong4, GUO Ya-Dong1, SONG Liu-Dong
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1. School of Pharmaceutial Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming
650500, China;2. State Key Laboratroy of Phytochemistry and Plant Resources in Western China, Kunming Institute of Botany, Chinese
Academy of Sciences, Kunming 650201, China;3. College of Rehabilitation and Nursing, Yunnan Business Management College,
Kunming 650106, China;4. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute
of Botany Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China
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| Abstract: |
| To study the diterpenoids from Calocedrus macrolepis and their anti-inflammatory activities, the constituents were separated by silica gel, MCI, RP-18, semi-preparative HPLC and eight compounds were isolated. Their structures were characterized as: 8-hydroxy-labda-13(16),14-dien-19-yl trans-coumarate(1), trans-communal(2), trans-communic acid(3), pinusolidic acid(4), isocupressic acid(5), fokihodgin F(6), acetylisocupressic acid(7), 15,16-dihydroxy-labda-8(17),13(E)-dien-19-oic acid(8). Compounds 1-8 were obtained from this plant for the first time. Among them, Compound 7 exhibited potent inhibitory effect on NO production in LPS-induced macrophages with IC50 values of 9.31 μmol·L-1. |
| Key words: Cupressaceae, Calocedrus, C. macrolepis, diterpenoids, constituents, anti-inflammatory activity |
