| 摘要: |
| 油质蛋白基因对种子中油体的形成至关重要,该研究通过实时荧光定量PCR,对麻疯树的两个油质蛋白基因JcOle14.3和JcOle16.6在种子不同发育时期的表达模式进行了分析。结果表明:两个基因在种子发育初期(10～30 d)表达量逐渐升高,但表达水平均较低; 40 d时表达量急剧增加并达到最高,而种子发育后期(50～55 d)两个基因表达水平均逐渐降低。由此可初步推测,JcOle14.3和JcOle16.6基因的表达量可能与种子油脂积累量存在正相关。该研究结果为麻疯树油体形成机理和油质蛋白的深入研究提供了理论基础。 |
| 关键词: 麻疯树, 种子, 油质蛋白, 荧光定量, 表达分析 |
| DOI：10.11931/guihaia.gxzw201612013 |
| 分类号:Q943.2, |
| 文章编号:1000-3142(2017)09-1096-05 |
| Fund project:云南省生物学优势特色重点学科建设项目(50097505); 云南省高校林下生物资源保护及利用科技创新团队项目(51400605)[Supported by the Key Discipline Construction Project of Biology in Yunnan Province, China( 50097505); Science and Technology Innovation Team for Forest Biological Resources Protection and Utilization of Yunnan Province Colleges and Universities(51400605)]。 |
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| Expression patterns of JcOle14.3 and JcOle16.6 in different developmental stages of Jatropha curcas seeds |
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YU Jin-De, XIONG Hong, SONG Jian, CHEN Hai-Tao, LIU Xiao-Zhu, DING Yong*
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College of Life Sciences, Southwest Forestry University, Kunming 650224, China
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| Abstract: |
| The oleosin gene is very important to the formation of oil body in seeds, present study analyzed the expression patterns of oleosin genes JcOle14.3 and JcOle16.6 in different development stages of Jatropha curcas seeds by quantity RT-PCR, and the results showed that the expression levels of JcOle14.3 and JcOle16.6 were increased gradually at the early development stage(10-30 d)of seeds, but the levels were low. However, on the 40th day after pollinated, the expression levels of these two genes increased dramatically and arrived the highest, and the levels were reduced at the late development stage(50-55 d)of seeds. The results could be presumed that the expression 1evels of JcOle14.3 and JcOle16.6 may have the positively correlation with the lipid accumulation in the seeds. |
| Key words: Jatropha curcas, seeds, oleosin, quantity RT-PCR, expression analysis |
