| 摘要: |
| 花色是观赏植物重要的观赏性状之一,而类黄酮是其主要的呈色物质。该研究以蓝亚麻花瓣为研究对象,将蓝亚麻开花过程分为5个阶段,并用高效液相色谱—光电二极管阵列检测技术(HPLC-PAD)和高效液相色谱—电喷雾离子化—质谱连用技术(HPLC-ESI-MS)分析不同开花阶段花瓣中类黄酮化合物的成分和含量。结果表明:蓝亚麻花瓣中积累飞燕草素苷、矢车菊素苷和锦葵素苷,未检测到天竺葵素苷,其中以酰基化的飞燕草素苷为主要呈色物质; 而总花青素苷含量在第2阶段达到最高。根据花青素苷终产物和类黄酮中间代谢产物推定了蓝亚麻花瓣中类黄酮代谢途径,其中以F3'5'H所引导的分支途径占优势,其主要原因可能是F3'5'H酶活高于F3'H。 |
| 关键词: 蓝亚麻, 花青素苷, 类黄酮, 花色, 代谢途径 |
| DOI：10.11931/guihaia.gxzw201612020 |
| 分类号:Q946.8 |
| 文章编号:1000-3142(2017)11-1368-10 |
| Fund project:国家自然科学基金(31560568),云南省人才培养基金(KKSY201323131)[Supported by the National Natural Science Foundation of China(31560568); Program Training Talents in Yunnan Province(KKSY201323131)]。 |
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| Flavonoid related compounds and their biosynthesis pathways in Linum pernne |
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SONG Yang2, LEI Ting2, JIN Xue-Hua1*, FU Deng-Xiang1
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1. Faculty of Architecture and City Planning, Kunming University of Science and Technology, Kunming 650500, China;2. Faculty
of Modern Agricultural Engineering, Kunming University of Science and Technology, Kunming 650500, China
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| Abstract: |
| Flower colour is one of the most ornamental quality in ornamental plant, and flavonoid is the main pigment. In this study, the flowering stages of Linum pernne was divided into five stages. We used high performance liquid chromatography with a photodiode array detector(HPLC-PAD)and HPLC-electrospray ionization-mass spectrometry(HPLC-ESI-MS)method for qualitative and quantitative analyses of anthocyanins and the key flavonoid intermediate product in the different flower development stages. The results were as follows: L. pernne petals contained delphinidin, cyanidin and malvidin, but pelargonidin was no detected, and the main color substance was acylation of delphinidin; The highest total contents of the anthocyanins was at Stage 2. According to the end product and the intermediate product, we speculate the flavonoid biosynthesis pathways in L. pernne, in which F3'5'H leading branch pathway is dominant, and the main reason for that is F3'5'H enzyme activity is higher than that of F3'H. |
| Key words: Linum pernne, anthocyanin, flavonoid, flower colour, biosynthesis pathways |
