| 摘要: |
| 春性甘蓝型油菜杂交种由于发育期较长,极大地限制了该油菜在高寒地区的推广。为了从春性特早熟甘蓝型油菜中筛选出苗期和蕾期差异表达的基因,该研究以春性特早熟甘蓝型油菜为材料,利用cDNA-AFLP技术筛选出1个春性特早熟甘蓝型油菜苗期特异表达的基因片段,并采用RACE技术成功分离克隆了该基因,命名为BnFY34。通过对该基因的测序和生物信息学分析,发现该基因序列大小为455 bp,包含完整的开放阅读框(ORF),编码一个由71个氨基酸残基组成的蛋白,推测的蛋白质分子量为8.04 kD,理论等电点为10.25,其三级结构中含有3个α螺旋,不含β折叠。同时,将BnFY34基因与甘蓝型油菜基因组序列进行比对,结果显示BnFY34基因位于C4染色体上,由3个外显子和2个内含子组成。另外,通过对该基因与其他植物同源基因的亲缘关系进行了分析,结果表明BnFY34基因与芸薹属植物属于同一亚族,并且与甘蓝型油菜中已报到的未诠释基因XM_013837282.1的相似度达100%,其功能有待进一步研究。该研究结果对春油菜产量和品质的早熟转基因育种具有重要意义。 |
| 关键词: 甘蓝型油菜, 发育, 基因表达, cDNA-AFLP |
| DOI：10.11931/guihaia.gxzw201702004 |
| 分类号:Q943.2 |
| 文章编号:1000-3142(2018)01-0084-06 |
| Fund project:国家自然科学基金(31472135,31672475); 青海省海南州科技合作项目(2015-HZ-01); 三江源生态与高原农牧业国家重点实验室开放课题(2017-KF-02)和自主课题(2017-ZZ-09); 青海省重点实验室发展专项(2014-Z-Y01); 青海省创新平台建设专项(2017-ZJ-Y20); 青海省自然科学基金(2017-ZJ-940Q,2016-ZJ-910,2016-ZJ-957Q)[Supported by the National Science Foundation of China(31472135,31672475); Science and Technology Cooperation Program of Hainan Prefecture of Qinghai Province(2015-HZ-01); Open Program(2017-KF-02)and Independent Program(2017-ZZ-09)of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University; Special Fund for the Development of Key Laboratory Program of Qinghai Province(2014-Z-Y01); Special Fund for Innovation Platform Program, Qinghai Province(2017-ZJ-Y20); National Science Foundation of Qinghai Province(2017-ZJ-940Q,2016-ZJ-910,2016-ZJ-957Q)]。 |
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| Clone and sequence analysis on gene BnFY34 from spring rapeseed(Brassica napus) |
|
ZHAO Hongkui1,2, YIN Hengxia3*, HU Guilian1, LUO Yuxiu4,
ZHOU Huakun2, SHI Guoxi2, YAO Buqing2
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1. Hainan State Ethnic Minority Science and Information Center, Qiabuqia 813099, Qinghai, China;2. Northwest Plateau Institute of Biology,
Chinese Academy of Sciences, Key laboratory of restoration ecology of cold area in Qinghai Province, Xining 810008, China;3. State Key
Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China;4. College of Ecological
Environment and Engineering, Qinghai University, Xining 810016, China
|
| Abstract: |
| Rape, belonging to Brassica(Cruciferae), is an important oil crop widely cultivated in China due to its wide adaptability. Spring rapeseed(Brassica napus)is widely cultivated in Qinghai Province due to its high yield and quality, however, it has been greatly restricted the promotion of rape in alpine region because of its long development period. Therefore, screening differentially expressed genes in seedling and bud stages plays an important role in early seedling breeding of spring rape with high yield and quality. In this study, a gene, named BnFY34, specifically expressed gene under seedling stage, was screened out with cDNA-AFLP, and successfully cloned in full-length 455 bp with RACE, including complete Open Reading Frame(ORF)and coding a protein with 71 amino acid residues. The protein has a molecular weight of 8.04 kD and a theoretical isoelectric point of 10.25. Furthermore, three α-helix and no β folding structures existed in the tertiary structure of protein BnFY34. The BnFY34 gene was compared with the genome sequence of Brassica napus. The results showed that the BnFY34 gene located in the C4 chromosome of B. napus and consisted of three exons and two introns. In addition, the genetic relationship between the gene BnFY34 and homologous genes in other plants showed that the gene BnFY34 was under the same subfamily as the Brassica species, and was similar to that of the untranslated gene XM_013837282.1 reported in B. napus up to 100%, but its function needs to be further studied. |
| Key words: Brassica napus, development, gene expression, cDNA-AFLP |
