| 摘要: |
| 该研究以墨兰‘绿墨素'(Cymbidium sinense ‘Lv mosu')和大花蕙兰‘世界和平'(Cymbidium hybridum ‘Shijieheping')杂交兰F1代原球茎为材料,采用浸泡法研究不同浓度秋水仙素和不同处理时间诱导植株加倍的效果。结果表明:0.03%秋水仙素处理72 h,诱导变异率为36%,死亡率为36%,诱导效果最佳; 多倍体植株与二倍体植株相比,表现为植株根部木质化加剧,植株矮壮,叶色深绿,叶片变厚、变宽,叶面粗糙,少部分有双叶脉、叶尖开裂、叶片扭曲,生长缓慢等; 且多倍体植株气孔大,气孔密度减小; 经流式细胞仪分析,二倍体墨兰 × 大花蕙兰F1代植株的荧光通道值为88,多倍体植株荧光通道值是176,多倍体植株为四倍体。该研究结果为培育兰花新品种奠定了基础。 |
| 关键词: 墨兰‘绿墨素' × 大花蕙兰‘世界和平'F1代, 原球茎, 秋水仙素, 多倍体诱导 |
| DOI：10.11931/guihaia.gxzw201706020 |
| 分类号:Q949.9, Q321 |
| 文章编号:1000-3142(2018)02-0188-07 |
| Fund project:云南省重点新产品开发项目(2012BB008); 云南省昆明市科学技术局重点项目(2015-1-N-00984)[Supported by the Key New Product Development of Yunnan Province, China(2012BB008); the Key Program of Kunming Bureau of Science and Technology of Yunnan Province, China(2015-1-N-00984)] 。 |
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| Polyploid induction in Cymbidium sinenthese ‘Lv mosu'× Cymbidium hybridum‘Shijieheping' F1 generation |
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SONG Lian, Yang Junxu, LIU Dan, LI Zhilin, WANG Yuying*
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Institute of Landscape Plants, Yunnan Agricultural University, Kunming 650201, China
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| Abstract: |
| Technique of mutation breeding in protocorm of Cymbidium sinense ‘Lv mosu'× C.hybridum ‘Shijieheping' F1 generation of hybrids was studied by means of tissue culture and chemical mutagenesis. The results showed that 0.03% colchicine treatment for 72 h, induced mutation rate was 36%, the mortality rate of 36%, the induced effect was the best; the polyploidy plant had more lignified roots than the diploid plants, the plants were dwarfed, the veins, leaves were dark green, the leaves were thickened and broadened, rough and double leaf twist, slow growth and so on, while the stomata and guard cells were larger and the stomatal density was decreased. DNA ploidy was analyzed by flow cytometry and the values of fluorescence channel were 88 in diploid plants and 176 in polyploid plants, the polyploid plants were tetraploid. |
| Key words: Cymbidium sinense ‘Lv mosu'× Cymbidium hybridum‘Shijieheping' F1 generation, protocorm, colchicine, polyploidy induction |
