| 摘要: |
| 该研究以沙柑(Citrus nobilis)以及其他的柑橘类共22个品种的花粉为材料,通过FEI Quanta 200场发射环境扫描电镜观察花粉的赤道面、极面、外壁纹饰等形态特征,并对沙柑的分类地位及亲缘关系进行了分析。结果表明:沙柑花粉为中等型、形状为长球形,存在4条孔沟,极轴长为(29.98 ± 1.18)μm,赤道轴长为(22.32 ± 1.03)μm; 外壁纹饰为网状纹饰,网眼为圆形或者近圆形,网眼直径大小为(0.89 ± 0.10)μm,网脊平宽光滑不隆起,网脊宽度为(0.62 ± 0.07)μm。经过花粉形态比较并进行聚类分析,结果显示沙柑应为杂种起源,且与扁柑、年橘、广西红皮酸橘的花粉特征较为接近,在遗传距离0.442处聚为一组,推测四者具有一定的亲缘关系,且沙柑与后三者相比可能较为原始; 沙柑与岑溪酸橘、印度酸橘、姑婆山野生元橘、贺州野橘等可能存在一定的亲缘关系但亲缘关系较远,可结合分子标记等技术进行更深层次的研究。该研究结果为沙柑遗传演化、分类地位以及亲缘关系的鉴定提供了依据。 |
| 关键词: 沙柑, 花粉, 形态, 亲缘关系, 演化 |
| DOI：10.11931/guihaia.gxzw201710029 |
| 分类号:Q944.58 |
| 文章编号:1000-3142(2018)02-0250-10 |
| Fund project:国家自然科学基金(31160388); 广西自然科学基金重点项目(2013GXNSFDA019014); 广西科技研究与技术开发计划项目(桂科合1599005-2-15,桂科转1599004-6,桂科AD16380046); 广西柑橘创新团队(nycytxgxcxtd-05-01); 广西特聘专家专项; 国家现代农业(柑橘)产业技术体系项目(CARS-27); 桂林市科学研究与技术开发计划项目(20110307)[Supported by National Natural Science Foundation of China(31160388); Guangxi Natural Science Foundation(2013GXNSFDA019014); Scientific Research and Technology Development Program of Guangxi(1599005-2-15, 1599004-6, AD 16380046); Guangxi Citrus Innovation Team(nycytxgxcxtd-05-01); Guangxi Distinguished Experts Program; National Modern Agriculture(Citrus)Industrial Technology System Program(CARS-27); Scientific Research and Technology Development Program of Guilin(20110307)]。 |
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| Preliminary observation on pollen morphology of Shagan(Citrus nobilis) |
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TANG Yan1, WU Xiaoxiao1,2, DENG Chongling1*, LIU Binghao1, CHEN Chuanwu1, NIU Ying1
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1. Guangxi Key Laboratory of Citrus Biology, Guangxi Academy of Specialty Crops, Guilin 541004, Guangxi, China;2. College of Life Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China
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| Abstract: |
| Pollen morphology has a certain conservatism, so it is often used as a classification basis for plant resources. The observation of citrus pollen morphology can provide some references for citrus classification. The pollen of Shagan(Citrus nobilis)and other citrus material was observed by FEI Quanta 200 field emission scanning electron microscopy. The morphological characteristics of pollen equatorial view, polar view and exine ornamentation were analyzed by the SPSS and NTsys software. And their genetic relationship was analyzed. The results showed that the pollen of C. nobilis is 4-colpate type and it had round or roughly round pollen mesh. The pollen of equatorial axis of C. nobilis was(29.98 ± 1.18)μm, the polar axis was(22.32 ± 1.03)μm, the mesh ridge was(0.62 ± 0.07)μm; and the mesh size was(0.89 ± 0.10)μm. The pollen morphology was compared with the results of cluster analysis. The results showed that the C. nobilis should be the origin of hybrids. The pollen characteristics of Biangan, Nianju, Guangxihongpisuanju and C. nobilis were similar, and the genetic distance was 0.442, which suggested that the four had a certain genetic relationship, and C. nobilis may be more primitive. There are some phylogenetic relationships in the world, such as Cenxisuanju, Yindusuanju, Guposhanyeshengyuanju and Hezhouyeju, which can be further studied with molecular markers. This study provides information for genetic evolution, taxonomic status and relative relationship of C. nobilis. |
| Key words: Shagan(Citrus nobilis), pollen, morphology, relative relationships, evolution |
