鸭儿芹的化学成分及对Hep G2细胞毒性

Chemical constituents of Cryptotaenia japonica and toxicity to Hep G2

关键词:  鸭儿芹, 化合物, 抗肿瘤活性

Key words:  Cryptotaenia japonica, compond, anti-tumor activity

摘要:

该研究采用大孔树脂(D101)、硅胶、羟丙基葡聚糖凝胶(Sephadex LH-20)和十八烷基硅烷键合硅胶(ODS)等色谱方法,对鸭儿芹的化学成分进行了分离纯化,根据理化性质、质谱和核磁共振波谱数据,并参考相关文献综合分析化合物结构,进而采用噻唑蓝(MTT)法,对鸭儿芹化合物抗Hep G2细胞活性进行筛选。结果表明:共从鸭儿芹中分离鉴定了7个化合物,分别为p-(acetylamino)phenol(1),辛酸甲酯(2),丁酸异戊酯(3),N,N-二甲基-苯并咪唑-2胺(4),5-羟基-1-(4-羟基-3-甲氧苯基)庚3酮(5),3,5二丁基六氢吡咯里嗪(6),(S)-4-(1-hydroxyallyl)phenyl acetate(7)。其中,化合物6对细胞具有抑制作用,抑制率达到89.1%。该研究结果表明化合物1-7均为首次从鸭儿芹中分离得到,其中化合物6对Hep G2细胞的生长具有抑制作用,且具有剂量依赖性。

Abstract:

Chemical constituents of Cryptotaenia japonica were separated and purified by macroporous resin D101, silica gel chromatography, Sephadex LH-20 and octadecylisilyl(ODS). Component structures were identified by physical and chemical properties, using spectral analysis included MS and NMR. MTT method was used to evaluate anti-tumor activities of chemical components. The results were as follows: Seven constituents were obtained from C. japonica. The structures were identified as p-(acetylamino)phenol(1), methyl caprylate(2),isopentyl butyrate(3), N,N-dimethyl-1H-benzo [d]imidazol-2-amine(4),5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)heptan-3-one(5),(3R*,5S*,7aR)-3,5-dibutylhexahydro-1H-pyrrolizine(6),(S)-4-(1-hydroxy-allyl)phenyl acetate(7). Componds 1-7 were obtained for the first time from C. japonic. Component 6 showed significant inhibitory effect against Hep G2 with the inhibition ratio 89.1% and had dose-dependent effect.