瑶药冷骨风质量控制方法研究

Quality control method for Yao medicine Nuphar pumilum

关键词:  瑶药, 冷骨风, 性状, 显微鉴别, 薄层色谱, 含量测定

Key words:  Yao medicine, Nuphar pumilum, traits, microscopic identification, TLC, content detemination

摘要:

瑶药冷骨风来源于睡莲科植物萍蓬草(Nuphar pumilum)的干燥根茎,为瑶族人习用药材,具有止咳补虚、除蒸止汗、祛瘀调经、止血的作用,目前历版《中国药典》和地方标准并未有收载其质量控制方法。该研究收集了10批冷骨风药材,采用生药鉴别方法对冷骨风药材性状进行显微鉴别,用TLC(薄层色谱)法对药材进行定性鉴别,用高效液相色谱法对没食子酸含量进行测定。结果表明:(1)色谱柱为Welch Ultimate XB-C₁₈柱(4.6 mm × 250 mm,5 μm),流动相为甲醇-0.05%磷酸溶液(5:95),流速为1.0 mL·min^-1,检测波长为272 nm,柱温为室温。(2)建立了冷骨风药材性状、显微、TLC鉴别方法。(3)含量测定中没食子酸在0.072 1～0.360 5 μg范围时,进样量与峰面积呈良好的线性关系,平均回收率为103.66%,RSD为2.03%(n=6)。(4)10 批样品没食子酸含量为0.82%～1.23%,平均含量为0.94%。因此,建立的瑶药冷骨风质量控制方法具有较强的科学性,可用于冷骨风药材的质量控制。

Abstract:

Yao medicine Lenggufeng is the dried rhizome of Nuphar pumilum, which was commonly used by Yao people. The herb medicine can be widely used for relieving cough, reinforcing deficiency, eliminating steam, hidroschesis, removing blood stasis, regulating menstruation and hemostasis. But the method for quality control of N. pumilum is not recorded in different versions of Chinese Pharmacopoeias or local quality standard of herbal medicine. Ten batches of herb medicine were collected in this study. The traits and microscopes of Nuphar pumilum were identified by crude drug identification method, the qualitative determination were performed by TLC, and the contents of gallic acid in these samples were determined by HPLC. The results were as follows:(1)The column was Welch Ultimate XB-C₁₈(4.6 mm × 250 mm, 5 μm)with mobile phase of methanol-0.05% phosphoric acid(5:95)at a flow rate of 1.0 mL·min^-1, detection wavelength was 272 nm, column temperature was room temperature.(2)The identification method for the traits, microscopes and TLC were established.(3)The gallic acid showed a good linear relationship within the range of 0.072 1-0.360 5 μg, whose average recovery(n=6)was 103.66%, with the RSD of 2.03%.(4)Gallic acid contents for the ten batches of samples were 0.82%-1.23%, and the average content was 0.94%. This established quality control method has strong scientificity and can be used for the quality control of N. pumilum.