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| 湿地松×洪都拉斯加勒比松遗传多样性的ISSR分析(英文) |
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李义良1,2*, 赵奋成1,2, 李福明3, 钟岁英3, 谭志强3, 吴惠姗1,2, 郭文冰1,2, 廖仿炎1,2
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1. 广东省森林培育与保护利用重点实验室, 广州 510520;2. 广东省林业科学研究院,
广州 510520;3. 台山市红岭种子园, 广东 台山 529200
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| 摘要: |
| 杂交育种是产生遗传变异、表型变异及选择新变异的重要方法。然而系统发育不清晰,选择较近的亲缘关系亲本用于杂交子代往往表现出较低的遗传多样性。为探究湿地松、洪都拉斯加勒比松种间杂交后代遗传多样性水平,对8个湿地松×洪都拉斯加勒比松家系进行ISSR分析。利用10条引物共产生60个表达清晰可用于分析的标记,其中48个标记表现为多态性,占总标记数的80%; 湿加松各个家系多态位点百分率在5%～23.33%之间; 各个家系基因多样性指数在 0.015 2～0.087 2之间,Shannon指数的范围在 0.021 6～0.129 4之间(家系水平为 0.293 4)。8个家系间的基因分化系数Gst为0.743 5,即总的遗传变异中有74.35%的变异存在于家系间,家系内的遗传变异占总遗传变异的25.65%。采用UPGMA法对湿加松的8个家系进行了聚类分析,确定了各个家系之间的遗传亲缘关系。8个家系间的基因流Nm为 0.172 5,表明基因流处于较低水平。 |
| 关键词: 湿加松, 家系, 遗传多样性, ISSR标记 |
| DOI：10.11931/guihaia.gxzw201704009 |
| 分类号: |
| 文章编号:1000-3142(2018)06-0812-06 |
| Fund project:广东省林业科技创新专项项目(2016KJCX021)[Supported by the Specific Programs of Forest Science and Technology Innovation in Guangdong(2016KJCX021)]。 |
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| Genetic diversity analysis of Pinus elliottii × P. caribaea var. hondurensis using ISSR markers |
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LI Yiliang1, 2*, ZHAO Fencheng1, 2, LI Fuming3, ZHONG Suiying3, TAN Zhiqiang3,
WU Huishan1, 2, GUO Wenbing1, 2, LIAO Fangyan1, 2
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1. Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangzhou 510520, China;2. Guangdong Academy
of Forestry, Guangzhou 510520, China;3. Taishan Hongling Seed Orchard, Taishan 529200, Guangdong, China
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| Abstract: |
| Cross-breeding is an important method for generating genetic and phenotypic variation for selecting new varieties. However, because of an uncertainty of phylogenetic relationships, the parents selected for crosses may have a close genetic relationship resulting in hybrid progeny that shows low genetic diversity. Analysis of inter-family genetic diversity was undertaken among eight Pinus elliottii × P. caribaea var. hondurensis full-sib families using inter-sequence simple repeat(ISSR)markers. A total of 480 individuals were analyzed using 10 ISSR primers. Nei's unbiased gene diversity in the families ranged from 0.015 2 to 0.087 2. Shannon genetic diversity index values ranged from 0.021 6 to 0.129 4. Only a small proportion(25.65%)of genetic variation resided within families, whereas the majority of genetic variation(74.35%)accounted for the inter-family genetic differentiation index of Gst=0.743 5. On the basis of estimated genetic distance and UPGMA clustering analysis, the genetic differentiation among the eight families was indicated to be relatively high with low gene flow(Nm=0.172 5). The low inter-family gene flow may be related to the high genetic heterozygosity of slash pine and Caribbean pine. These findings are expected to provide a foundation for genetic breeding of Pinus elliottii × P. caribaea var. hondurensis hybrids. |
| Key words: Pinus elliottii × P. caribaea var. hondurensis, ｆａｍｉｌｉｅｓ, ｇｅｎｅｔｉｃ ｄｉｖｅｒｓｉｔｙ, ＩＳＳＲ ｍａｒｋｅｒ |
