冷饭团不同部位挥发性成分及抗氧化活性分析

Volatile components and their antioxidant activities in different parts of Kadsura coccinea

关键词:  冷饭团植株, GC-MS, SPME, 抗氧化活性, 挥发性成分

Key words:  Kadsura coccinea, GC-MS, SPME, antioxidant activity, volatile component

摘要:

为较全面地认识冷饭团(Kadsura coccinea)植株挥发性成分及其抗氧化活性,该研究以其根、茎、叶为材料,采用水蒸气蒸馏提取法(方法a)和顶空-固相微萃取法(SPME)(方法b)结合气相色谱-质谱联用技术(GC-MS)与计算机检索技术,对冷饭团植株各部位挥发性成分进行了提取分析,并采用DPPH法对其抗氧化活性进行测试。结果表明:(1)两种提取方法检测出的挥发性成分种类及含量存在一定差异,用方法a和方法b分别在根、茎、叶中共鉴定出挥发性化合物98和117种; 其中根中分别有59与68种(占总挥发性物质的83.5%, 93.8%)、 茎中54和56种(占总挥发性物质的88.9%, 94.9%)、叶中36和42种(占总挥发性物质的89.6%, 97.7%)。(2)根、茎、叶中两种方法检出共同化合物种类及含量基本相同,主要有石竹烯、蒎烯、榄香烯、毕澄茄油烯、古巴烯、毕澄茄烯、d-苦橙花醇等,这些成分也是各部分挥发物的主要成分(含量超过70%)。(3)根、茎、叶中挥发性物质质量浓度达到20 mg·mL^-1时,清除DPPH自由基效率均超过97%,表现出很好的抗氧化活性。

Abstract:

Volatile components antioxidant activities of Kadsura coccinea and were comprehensively studied. The volatile compounds from three different parts which were roots, stems and leaves of K. coccinea were extracted by steam distillations(Method a)and headspace solid phase micro-extraction(SPME)(Method b). Volatile components of K. coccinea were analyzed by combining gas chromate-graphy-mass spectrometry(GC-MS)and the technology of computer retrieva. Antioxidant activities of volatile components were tested by DPPH free radical scavengng activity. The results were as follows:(1)There were some differences between species and contents of volatile components. And 98 and 117 volatile compounds were identified from three parts of K. coccinea through methods a and b. Among them, there were 59 and 68 kinds volatile components(accounting for 83.5%, 93.8% of root total content)in root, 54 and 56 species volatile components(accounting for 88.9%, 94.9% of stem total content)in the stem and 36 and 42 species in the leaf(accounting for 89.6%, 97.7% of leaf total content).(2)Species and contents of volatile components of three parts of K. coccinea through methods a and b were basically the same. The main volatile components were caryophyllene, pinene, elemene, cubebene, copaene, cadinene and d-Nerolidol(contents ≥70%).(3)Scavenging efficiency for DPPH free radical was more than 97% when the concentration of volatile components of root, stem and leaf of K. coccinea reached 20 mg·mL^-1, and volatile components of K. coccinea showed good antioxidant activity.