植物凝胶和蔗糖对橡胶树体胚植株再生的影响

Effects of phytagel and sucrose on regeneration efficiency of somatic embryos and growth of regenerated plants in Hevea brasiliensis

关键词:  植物凝胶, 蔗糖, 胚状体, 橡胶树, 植株再生

Key words:  phytagel, sucrose, embryos, rubber tree, plant regeneration

摘要:

为了筛选巴西橡胶体胚植株最为适合的植物凝胶和蔗糖用量,该研究以巴西橡胶树无性系热研7-33-97成熟体细胞双子叶次生胚状体为材料,以添加0.23 μmol·L^-1 KT,0.11 μmol·L^-1 IAA和8.7 μmol·L^-1 GA3的MS培养基为植株再生培养基,研究了添加不同用量的植物凝胶和蔗糖,对巴西橡胶树体胚植株再生和生长的影响。结果表明:在橡胶树体细胞胚植株再生培养基中,不同用量的植物凝胶对植株再生频率和植株生长状况有显著影响,较低浓度(0～1 g·L^-1)时,随着用量增加,植株再生频率提高,但较高浓度(1～4 g·L^-1)时,随着用量增加,植株生长受到抑制。植物凝胶添加1 g·L^-1时植株生长最好,植株再生率为(86.4±5.7)%,株高5 cm以上的占(53±9.4)%,带叶植株为(81.7±3)%; 而蔗糖对植株再生频率影响不显著,但对再生植株生长的影响显著,低蔗糖(20～30 g·L^-1)时促进植株抽叶但抑制茎干伸长,高蔗糖(70～80 g·L^-1)时显著抑制抽叶但促进茎干伸长。蔗糖添加50 g·L^-1时植株生长最好,株高5 cm以上的占(57.6±5.4)%,株高5 cm以上带叶植株占(46.3±12.3)%,均为最高且从外观来看,在50 g·L^-1时植株茎干和根都较为粗壮。因此,在橡胶树体细胞胚植株再生培养基中,植物凝胶和蔗糖最佳用量分别为1 g·L^-1和50 g·L^-1。

Abstract:

Phytagel and sucrose have great influence on the regeneration efficiency of somatic embryos and growth of regenerated plants. In this study, effects of phytagel and sucrose on the regeneration of somatic embryos and growth of regenerated plants were carried out on mature cotyledonary embryos from Hevea brasiliensis clone Reyan7-33-97. MS me-dium supplemented with 0.23 μmol·L^-1 KT, 0.11 μmol·L^-1 IAA and 8.7 μmol·L^-1 GA3 was used as the plant regeneration medium. The results showed that appropriate phytagel was helpful for the regeneration efficiency of somatic embryos and growth of regenerated plants. At lower concentrations(0-1 g·L^-1), the frequency of plant regeneration increased with the increase of the amount of phytagel, but at higher concentrations(1-4 g·L^-1), plant growth was inhibited along with the increasing amounts of phytagel. When the phytagel was supplemented in somatic embryo regeneration medium with 1 g·L^-1, the plant regeneration rate, the plants(height >5 cm)and the plants with leaves reached(86.4 ± 5.7)%,(53 ± 9.4)% and(81.7 ± 3)%, respectively, higher than other treatments; For sucrose, no effect on the regeneration efficiency of somatic embryos was observed when sucrose was supplemented in somatic embryo regeneration medium, however, significant effects on growth of regenerated plant were observed. When the sucrose was supplemented in somatic embryo regeneration medium with 50 g·L^-1, the plants(height >5 cm)and the plants with leaves(height >5 cm)reached(57.6±5.4)% and(46.3±12.3)%, respectively, higher than other treatments, and the stems and roots were very strong from the outside. Moreover, lower sucrose(20-30 g·L^-1)promoted the growth of leaves as plant sprouting, but the elongation of stem was supressed, in contrast, higher sucrose(70-80 g·L^-1)suppressed the growth of leaves but promoted the elongation of the stem. Overall, the somatic embryo regenaration medium supplimented with 1 g·L^-1 phytagel and 50 g·L^-1 sucrose was the most approciate for somatic embryo regenaration in H. brasiliensis.