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| 广西沿海四种红树植物抗肿瘤活性的筛选 |
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谭德超1,2, 罗 花1, 邓家刚1,3, 郝二伟1,3, 易湘茜1,2,3, 冯小慧1,2,
韦林垚1,2, 夏中尚1,2, 徐炜杰1,2, 谢金玲1,3, 侯小涛1,2,3*
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1. 广西中医药大学 中药药效研究重点实验室, 南宁 530200;2. 广西中医药大学 药学院, 南宁 530200;3. 广西中医药大学 农作物废弃物功能成分研究协同创新中心, 南宁 530200
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| 摘要: |
| 该研究采用超声提取法,得到红海榄的叶、秋茄的茎和叶、水黄皮的叶和桐花树的茎的95%乙醇提取物,并用MTS法检测提取物对前列腺癌DU145、PC3细胞增殖抑制效果,结果桐花树茎95%乙醇提取物对DU145细胞增殖抑制作用最强,对DU145干预24、48、72 h的IC50分别为75.23、88.81、76.53 μg·mL-1。在此基础上,依次采用石油醚、乙酸乙酯、正丁醇对桐花树茎95%乙醇提取物进行梯度萃取,得到不同极性部位后选择桐花树茎乙酸乙酯部位(EACS)对19种肿瘤细胞(HT-29,SW480,DLD-1,COLO205,PC3,DU145,SKOV3-S,A2780,SGC-7901,Tca-8113,MDA-MB-231,HepG2,SMMC-7721,Bel-7402,MHCC-97H,Hela,PANC-1,EJ,A549)和3种正常细胞(HUVEC,EC304,RWPE-1)进行MTS实验检测其抗增殖作用,用集落形成进一步检测EACS对细胞HT-29、DLD-1、SW480、DU145、PC3、SKOV3-S的增殖影响。结果表明:EACS对16个肿瘤细胞和3个正常细胞均具有不同程度的增殖抑制作用,其中对RWPE-1的增殖抑制作用最强,药物作用72 h后的IC50为22.78 μg·mL-1; EACS对细胞HT-29、DLD-1、SW480、DU145、PC3、SKOV3-S的集落形成均具有抑制作用,抑制作用强度与浓度成正相关关系。 |
| 关键词: 红树植物, 抗肿瘤活性, 筛选, MTS, 集落形成 |
| DOI：10.11931/guihaia.gxzw201805022 |
| 分类号:R932 |
| 文章编号:1000-3142(2018)10-1267-10 |
| Fund project:国家自然科学基金(21662006); 广西科技计划项目(桂科AD17195025); 广西科学研究与技术开发计划项目(桂科合15104001-11); 广西教育厅中药学优势学科建设项目(ZYXZ2015001)[Supported by the National Natural Science Foundation of China(21662006); Guangxi Science and Technology Program(AD17195025); Guangxi Science and Technology Construction Planning(15104001-11); Key Program for Special Subject Construction of Chinese Materia Medica in Guangxi Education Department(ZYXZ2015001)]。 |
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| Screening antitumor activity of four mangrove plants in Guangxi coastal area |
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TAN Dechao1,2, LUO Hua1, DENG Jiagang1,3, HAO Erwei1,3, YI Xiangxi1,2,3, FENG Xiaohui1,2,
WEI Linyao1,2, XIA Zhongshang1,2, XU Weijie1,2, XIE Jinling1,3, HOU Xiaotao1,2,3*
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1. Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning 530200, China;2. Faculty of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China;3. Collaborative Innovation Center of Study
on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning 530200, China
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| Abstract: |
| Ninety-five percent of ethanol extracts from the leaves of Rhizophora stylosa, stems and leaves of Kandelia candel, leaves of Pongamia pinnata and stems of Aegiceras corniculatum were extracted by ultrasonic extraction method. The cytotoxicity of 95% ethanol extracts from these four mangrove plants on prostate cancer cells DU145 with 24, 48 and 72 h and PC3 were tested by MTS assay. The results showed that the anti-proliferation on prostate cancer cell DU145 of 95% ethanol extract of A. corniculatum stems was the strongest among the tested samples with IC50 value of 75.23, 88.81 and 76.53 μg·mL-1 to DU145, respectively. On this basis, the ethanol extract of A. corniculatum stems was successively treated with petroleum ether, ethyl acetate and n-butanol to yield four fractions, then the ethyl acetate fraction of A. corniculatum stems(EACS)were tested for anticancer activities against nineteen tumor cells(HT-29, SW480, DLD-1, COLO205, PC3, DU145, SKOV3-S, A2780, SGC-7901, Tca-8113, MDA-MB-231, HepG2, SMMC-7721, Bel-7402, MHCC-97H, Hela, PANC-1, EJ, A549)and antiproliferative effects of three normal cells(HUVEC, EC304, RWPE-1)by MTS assay, the effects of EACS on proliferation of HT-29, DLD-1, SW480, DU145, PC3 and SKOV3-S cells were detected by the colony formation assay. The results showed that EACS exhibited different degrees of inhibitory effect on proliferation of sixteen tumor cells and three normal cells, and had the stronger inhibitory effect on the proliferation of RWPE-1 with IC50 value of 22.78 μg·mL-1 after 72 h drug treatment. EACS inhibited the colony formations of HT-29, DLD-1, SW480, DU145, PC3 and SKOV3-S cells in a dose-dependent manner. |
| Key words: mangrove plants, antitumor activity, screening, MTS, colony formation |
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