| 摘要: |
| 为了从酸枣中筛选出内生菌并分析其代谢产物中的活性成分,用于开发和生产药物,该研究通过组织块分离法和划线分离法,从陕北野生酸枣植株体内分离得到内生菌,采用平板对峙法测定其对7株供试指示菌的抗菌活性,以心神宁片提取液为对照,对各拮抗菌株的发酵液进行薄层层析和高效液相色谱分析。结果表明:从野生酸枣中共分离得到121株内生菌,其中内生细菌49 株,内生放线菌6株,内生真菌66株; 通过抗菌试验,发现54株内生菌(细菌33株,真菌21株)对1～7种指示菌具有抗菌活性,占分离菌株总菌数的44.63%,其中A-04、A-05、B-03、C-03、C-06和D-04共6株菌株的抗菌谱较广,对7种供试指示菌均具有抑菌活性; 薄层层析检测结果显示菌株B-03 发酵产物在Rf值为0.46处有与酸枣提取液层析带迁移率相同的显色带,液相色谱分析结果显示其属于黄酮类物质; 通过16S rRNA基因序列分析结果显示菌株B-03与Bacillus axarquiensis的相似性为99%。菌株B-03能发酵产生黄酮类或产生与黄酮类类似的化合物,表明酸枣内生菌具有合成黄酮类药物的潜力。 |
| 关键词: 酸枣, 内生菌, 代谢产物, 黄酮类物质 |
| DOI：10.11931/guihaia.gxzw201712030 |
| 分类号:Q946 |
| 文章编号:1000-3142(2018)11-1486-07 |
| Fund project:陕西省科技统筹创新工程项目(2016TTC-N-3-1); 延安市科技局重大专项项目(2014CGZH-06); 陕西省教育厅服务地方专项计划项目(16JF029); 延安市菌草工程研究中心专项基金; 延安市生物资源开发与利用科技创新团队专项基金; 延安大学“陕北微生物资源与利用研究中心”科研机构专项基金; 延安大学产学研合作研发项目 [Supported by Science and Technology Coordinating Innovation Program of Shaanxi(2016TTC-N-3-1); Major Special Program of Yan'an City(2014CGZH-06); Service Local Special Plan Program of the Department of Education of Shaanxi Province(16JF029); Special Fund of Juncao Engineering Research Center of Yan'an City; Special Fund of Technology Innovation Team for the Development and Utilization of Biological Resources of Yan'an City; Special Fund Scientific Research Institutions “North of Shaanxi Microbial Resources and Utilization Research Center”of Yan'an University; Fund for Research Cooperative and Development Program in Yan'an University]。 |
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| Identification of an endophytic strain from Ziziphus jujube and preliminary separation of its antimicrobial active substances |
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DENG Zhenshan*, GAO Fei, LIU Yuzhen, WEI Tingting, LI Jing, Li Zhengting
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College of Life Sciences, Yan'an University, Yan'an 716000, Shaanxi, China
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| Abstract: |
| The purpose of this study was to identify endophytic strains from Ziziphus jujube and analyze the active components of their metabolites for the development and production of drugs. Wild Ziziphus jujube plants from North Shaanxi were adopted as experimental materials, and endophyte strains were isolated by approaches of tissue separation and streak plate, then they were screened and tested on the bacteriostatic activity by adopting indicators of seven strains from face-to-face cultivation in PDA media. The fermentation liquor of antagonistic strain was detected using thin layer, chromatography(TLC)and high performance liquid chromatography(HPLC). The experimental results showed that a total number of 121 strains of endophytes were isolated from Ziziphus jujube, including 49 strains of endophytic bacteria, 6 strains of endophytic actinomycetes, and 66 strains of endophytic fungi. Among of them, 54 strains(33 bacteria and 21 fungi)had obvious antagonistic activity against 1-7 indicative bacteria, accounting for 44.63% of the total, and six strains(A-04, A-05, B-03, C-03, C-06 and D-04)had a broad spectrum of antibacterial and antibacterial activity against seven strains of indicator microbes. Results from the analysis of TLC and HPLC showed that liquor in B-03 strain has the same color band with the migration rate of Ziziphus jujube extract in Rf value of 0.46, and its belonged to flavonoids. According to 16S rRNA gene sequences analysis. The similarity between strain B-03 and Bacillus axarquiensis was 99%. The strain of B-03 could produce flavonoids or similar compounds, which showed that the endophytes from Ziziphus jujube had the potential ability to produce new drugs of flavonoids. |
| Key words: Ziziphus jujube var. spinosa, endophyte, product of metabolism, flavonoids |
