大花蕙兰‘红酒'× 莲瓣兰‘边草素花'杂交种组培快繁技术

Tissue culture and rapid propagation techniques in Cymbidium hybridum ‘hongjiu'× C. tortisepalum ‘biancaosuhua'

关键词:  兰花杂交种, 根状茎, 原球茎, 增殖与分化

Key words:  Orchid hybrid, rhizome, protocorm, proliferation and differentiation

摘要:

该研究以大花蕙兰‘红酒'(Cymbidium hybridum ‘hongjiu')×莲瓣兰‘边草素花'(C. tortisepalum ‘biancaosuhua')F1代杂交种原球茎和根状茎为材料,比较了不同激素配比增殖分化、生根的培养基,建立了适用杂交兰组培快繁体系。结果表明:1/2MS+6-BA 1.0 mg·L^-1+NAA 1.0 mg·L^-1+AC 0.05%+香蕉80 g·L^-1对原球茎增殖效果最佳,增殖率达到307%; 1/2MS+6-BA 1.5 mg·L^-1+NAA 1.0 mg·L^-1 +AC 0.05%+香蕉80 g·L^-1有利于原球茎分化,分化率为82%; 1/2MS+TDZ 2.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.05%+香蕉80 g·L^-1对根状茎增殖分化效果最佳,增殖率为293%,分化率为79%; 1/2MS+IBA 0.5 mg·L^-1+NAA 0.3 mg·L^-1+AC 0.05%+香蕉80 g·L^-1为最佳生根培养基,生根率达到84.7%,且根粗苗壮,叶色浓绿。此体系为杂交兰种苗的规模化生产提供了技术支持。

Abstract:

A rapid propagation technique system of hybrid orchid was established by comparing the effects of different hormone ratios on proliferation and rooting, with the protocorms and rhizomes of F1 hybrids of Cymbidium hybridum ‘hongjiu' and C. tortisepalum ‘biancaosuhua' were used as materials. The results were as follows: 1/2MS+6-BA 1.0 mg·L^-1+NAA1.0 mg·L^-1+AC 0.05%+banana 80 g·L^-1 had the best conductive effects on proliferation of protocorm, and the proliferative rate reached to 307%; 1/2MS+6-BA 1.5 mg·L^-1+NAA 1.0 mg·L^-1+AC 0.05%+banana 80 g·L^-1 medium was beneficial to differential protocorm culture, and the differential rate was 82%; 1/2MS+TDZ 2.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.05%+banana 80 g·L^-1 medium was conductive to proliferation and differentiation of rhizome, and the proliferative rate reached 293%, the differential rate was 79%. Medium of 1/2MS+IBA 0.5 mg·L^-1+NAA 0.3 mg·L^-1+AC 0.05%+banana 80 g·L^-1 was the best for rooting, the rooting rate was 84.7%, and the roots were healthy with strong seedlings, and the leaves were dark green. This system provides technical support for large-scale production of hybrid orchid seedlings.