| 摘要: |
| 金铁锁是 “云南白药”等多种中成药的重要组成,其有效成分为三萜皂苷,MYC类转录因子在调节植物三萜类次生代谢积累中有重要作用。为研究ptMYC2 基因在金铁锁三萜皂苷合成代谢途径的调控机制,该研究基于金铁锁转录组测序数据,克隆得到ptMYC2转录因子的两个全长基因; 通过生物信息学软件对两条转录因子的同源性、理化性质、疏水性、跨膜结构、亚细胞定位、结构域、靶基因结合位点等进行初步预测分析。结果表明:两条转录因子所编码的蛋白属于亲水性蛋白,不存在跨膜区域,均是非分泌蛋白质,且不存在信号肽; 两条转录因子的亚细胞定位于细胞核; 结构域分析显示,两个基因都含有bHLH家族结构域; 预测得到金铁锁三萜皂苷合成途径中HMGR、FPS、SE、β-AS等基因的启动子可能存在与MYC2结合的E-box特异性结合位点。该研究结果将为进一步研究ptMYC2基因在金铁锁三萜皂苷合成代谢途径的调节机制奠定基础。 |
| 关键词: 金铁锁, 转录因子, MYC2, 三萜皂苷, 生物合成途径, 生物信息学分析 |
| DOI：10.11931/guihaia.gxzw201810005 |
| 分类号:Q943 |
| 文章编号:1000-3142(2019)10-1350-09 |
| Fund project:云南省科学技术厅-云南中医学院应用基础联合专项项目(2017FF117-001); 云南省科学技术厅-云南中医学院应用基础联合专项项目(2017FF117-030); 云南省应用基础青年项目(2015FD036)[Supported by Yunnan Provincial Department of Science and Technology-Yunnan University of Traditional Chinese Medicine Applied Foundation Joint Special Program(2017FF117-001); Yunnan Provincial Department of Science and Technology-Yunnan University of Traditional Chinese Medicine Applied Foundation Joint Special Program(2017FF117-030); Yunnan Applied Basic Youth Program(2015FD036)]。 |
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| Cloning and bioinformatics analysis of Psammosilene tunicoides transcription factor ptMYC2 |
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MENG Wenjun, ZHANG Aili, JIANG Lexiao, ZHU Wenjie, DUAN Li, QIAN Zigang*
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Certer for Reproducing Fine Varieties of Chinese Medicinal Plants, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China
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| Abstract: |
| Psammosilene tunicoides is an important component of various Chinese patent medicines such as “Yunnan Baiyao”, and its active ingredient is triterpenoid saponin. MYC2 is an important transcription factor, which plays an important role in regulating the secondary metabolic accumulation of triterpenoids in plants. In this study, we cloned two transcription factors( ptMYC2-1 and ptMYC2-2 )of Psammosilene tunicoides based on transcriptome sequencing. Meanwhile, the homology, physical and chemical parameters, hydrophobicity, transmembrane helices, subcellular location, domain, target gene binding site were predicted through bioinformatics software. The experimental results showed that the proteins encoded by the two transcription factors belonged to the hydrophilic protein and did not exist the transmembrane region, and both of them were non-secreted proteins, and without signal peptide. The subcellular of two transcription factors localizes to the nucleus. Domain analysis revealed that both genes contained the bHLH family domain. We predicted that the promoter of these genes( HMGR, FPS, SE and β-AS), which involved the triterpenoid saponin synthesis pathway of Psammosilene tunicoides, contained the E-box binding domain. This study provides the basic information for stu-dying the regulatory mechanisms of the ptMYC2 gene in the metabolic pathway of triterpenoid saponins. |
| Key words: Psammosilene tunicoides, transcription factor, MYC2, triterpenoid saponin, biosynthetic pathway, bioinformatics analysis |
