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| 黔产昆明山海棠化学成分及其抑菌活性研究 |
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李 江1,2,3, 李 2,3, 穆淑珍2,3, 张仕林4, 王莉云2,3, 郝小江2,3, 邓璐璐2,3*
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1. 贵州大学 药学院, 贵阳 550025;2. 贵州医科大学省部共建药用植物功效与利用国家重点实验室, 贵阳 550014;3. 贵州省中国科学院天然产物化学重点实验室, 贵阳 550014;4. 贵州汉方药业有限公司, 贵阳 550025
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| 摘要: |
| 该研究采用硅胶柱色谱、Sephadex LH-20 凝胶柱色谱、半制备型高效液相色谱和重结晶等方法分离纯化,从黔产昆明山海棠乙醇提取物中分离得到11个化合物,并采用96孔板微量稀释法对化合物进行抑菌活性测定。结果表明:利用 NMR,MS 等现代波谱技术以及化合物的理化性质并结合
参考文献分别鉴定为3-O-乙酰基齐墩果酸(1),雷酚萜(2),3-氧代齐墩果酸(3),β-谷甾醇(4),木栓酮(5),β-谷甾醇棕榈酸酯(6),雷公藤红素(7),大黄素(8),雷公藤内酯甲(9),雷藤二萜醌 B(10),ent-kauran-16 β,19-diol(11)。抑菌活性结果显示,化合物3、7和8具有较好的抑菌作用,MIC值为2～16 μg·mL-1。其中,化合物6、11为首次从该植物中分离得到,化合物11为首次从雷公藤属植物中分离得到,且首次发现了化合物3、7对绿脓杆菌和青枯菌具有明显的抑制作用。 |
| 关键词: 昆明山海棠, 雷公藤属, 雷公藤红素, 雷藤二萜醌 B, 抑菌作用 |
| DOI：10.11931/guihaia.gxzw201812038 |
| 分类号:Q946.91 |
| 文章编号:1000-3142(2019)11-1505-07 |
| Fund project:国家自然科学基金(81460530); 贵阳市科技计划项目(筑科合同 [20151001]药14号)[Supported by the National Natural Science Foundation of China(81460530); Guiyang Science and Technology Plan Program([20151001]14)]。 |
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| Chemical components of Tripterygium hypoglaucum from Guizhou Province and their antibacterial activites |
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LI Jiang1,2,3, LI Yan2,3, MU Shuzhen2,3, ZHANG Shilin4,
WANG Liyun2,3, HAO Xiaojiang2,3, DENG Lulu2,3*
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1. College of Pharmaceutical Science, Guizhou University, Guiyang 550025, China;2. State Key Laboratory of Functions and
Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China;3. Key Laboratory of Chemistry for
Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China;4. Guizhou Hanfang Pharmaceuticals Company, Guiyang 550025, China
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| Abstract: |
| Eleven compounds were isolated and purified from ethanol extract of Tripterygium hypoglaucum from Guizhou Province by silica gel column chromatography, Sephadex LH-20 gel column chromatography, semi-prepared HPLC and recrystallization. Their antibacterial activity were determined by 96-well plate microdilution method. The results were as follows: The structures were identified as 3-O-acetyloleanolicacetic anhydride(1), triptonoterpene(2), 3-Oxo-olean-12-en-28-oic acid(3), β-sitosterol(4), friedelin(5), β-sitosteryl palmitate(6), celastrol(7), emodin(8), wilforlide A(9), triptoquinone B(10), and ent-kauran-16 β, 19-diol(11)by NMR, MS, physicochemical properties and some reported data. Based on the results of antibacterial activity, compounds 3, 7 and 8 showed potential activity, and their MIC values were 2-16 μg·mL-1. Compounds 6 and 11 were isolated from this plant for the first time, and meanwhile compound 11 was also isolated from the genus of Tripterygium for the first time. Furthermore it was found firstly that compounds 3 and 7 had significant inhibitory effects on Pseudomonas aeruginosa and Rastonia solanacearum. |
| Key words: Tripterygium hypoglaucum, Tripterygium, celastrol, triptoquinone B, antibacterial activity |
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